scholarly journals Mapping CRISPR-Cas9 public and commercial innovation using The Lens institutional toolkit

2021 ◽  
Author(s):  
Osmat Azzam Jefferson ◽  
Simon Lang ◽  
Kenny Williams ◽  
Deniz Koellhofer ◽  
Aaron Ballagh ◽  
...  
Keyword(s):  
Public Investment ◽  
Open Data ◽  
Regulatory Elements ◽  
Policy Makers ◽  
Base Editing ◽  
The Public ◽  
Homology Directed Repair ◽  
A Genome ◽  
Institutional Capability ◽  
Cellular Dna

AbstractCRISPR-Cas9 is a revolutionary technology because it is precise, fast and easy to implement, cheap and components are readily accessible. This versatility means that the technology can deliver a timely end product and can be used by many stakeholders. In plant cells, the technology can be applied to knockout genes by using CRISPR–Cas nucleases that can alter coding gene regions or regulatory elements, alter precisely a genome by base editing to delete or regulate gene expression, edit precisely a genome by homology-directed repair mechanism (cellular DNA), or regulate transcriptional machinery by using dead Cas proteins to recruit regulators to the promoter region of a gene. All these applications can be for: 1) Research use (Non commercial), 2) Uses related product components for the technology itself (reagents, equipment, toolkits, vectors etc), and 3) Uses related to the development and sale of derived end products based on this technology. In this contribution, we present a prototype report that can engage the community in open, inclusive and collaborative innovation mapping. Using the open data at the Lens.org platform and other relevant sources, we tracked, analyzed, organized, and assembled contextual and bridged patent and scholarly knowledge about CRISPR-Cas9 and with the assistance of a new Lens institutional capability, The Lens Report Builder, currently in beta release, mapped the public and commercial innovation pathways of the technology. When scaled, this capability will also enable coordinated editing and curation by credentialed experts to inform policy makers, businesses and private or public investment.

Transparency in the Open Government Era

2013 ◽  
pp. 1-9
Author(s):  
Evika Karamagioli
Keyword(s):  
Information And Communication Technologies ◽  
Open Data ◽  
Open Government ◽  
Public Servants ◽  
Policy Makers ◽  
Innovative Strategy ◽  
The Public ◽  
Information And Communication ◽  
Governmental Institutions ◽  
The Public Sphere

The decline in citizen engagement in the public sphere has long been one of the main challenges of modern government. Issues of trust, openness, and transparency are being frequently and intensely discussed as the public manifests lack of confidence in public servants and governmental institutions. The advent and development of Information and Communication Technologies (ICTs) is gradually revolutionizing this situation. Open government is an innovative strategy for changing how government works, helping to increase government transparency and accountability at every level. By using network technology to connect the public to government and to one another informed by open data, governments ask for help with solving problems. The end result is more effective institutions and more robust democracy. The chapter has as an objective to discuss on a theoretical level the role of open government mechanisms in introducing a new relation between citizens and policy makers.

scholarly journals Reprogramming acetogenic bacteria with CRISPR-targeted base editing via deamination

2020 ◽  
Author(s):  
Peng-Fei Xia ◽  
Isabella Casini ◽  
Sarah Schulz ◽  
Christian-Marco Klask ◽  
Largus T. Angenent ◽  
...  
Keyword(s):  
Genome Editing ◽  
Dna Cleavage ◽  
Genetically Modified Organisms ◽  
Cytidine Deaminase ◽  
Base Editing ◽  
Homology Directed Repair ◽  
Clostridium Ljungdahlii ◽  
Acetogenic Bacteria ◽  
A Genome ◽  
Nucleotide Resolution

AbstractAcetogenic bacteria are rising in popularity as chassis microbes in biotechnology due to their capability of converting inorganic one-carbon (C1) gases to organic chemicals. To fully uncover the potential of acetogenic bacteria, synthetic-biology tools are imperative to either engineer designed functions or to interrogate the physiology. Here, we report a genome-editing tool at a one-nucleotide resolution, namely base editing, for acetogenic bacteria based on CRISPR-targeted deamination. This tool combines nuclease deactivated Cas9 with activation-induced cytidine deaminase to enable cytosine-to-thymine substitution without DNA cleavage, homology-directed repair, and donor DNA, which are generally the bottlenecks for applying conventional CRISPR-Cas systems in bacteria. We designed and validated a modularized base-editing tool in the model acetogenic bacterium Clostridium ljungdahlii. The editing principles were investigated, and an in-silico analysis revealed the capability of base editing across the genome. Moreover, genes related to acetate and ethanol production were disrupted individually by installing premature STOP codons to reprogram carbon flux towards improved acetate production. This resulted in engineered C. ljungdahlii strains with the desired phenotypes and stable genotypes. Our base-editing tool promotes the application and research in acetogenic bacteria and provides a blueprint to upgrade CRISPR-Cas-based genome editing in bacteria in general.SignificanceAcetogenic bacteria metabolize one-carbon (C1) gases, such as industrial waste gases, to produce fuels and commodity chemicals. However, the lack of efficient gene-manipulation approaches hampers faster progress in the application of acetogenic bacteria in biotechnology. We developed a CRISPR-targeted base-editing tool at a one-nucleotide resolution for acetogenic bacteria. Our tool illustrates great potential in engineering other A-T-rich bacteria and links designed single-nucleotide variations with biotechnology. It provides unique advantages for engineering industrially relevant bacteria without creating genetically modified organisms (GMOs) under the legislation of many countries. This base-editing tool provides an example for adapting CRISPR-Cas systems in bacteria, especially those that are highly sensitive to heterologously expressed Cas proteins and have limited ability of receiving foreign DNA.

scholarly journals Genomic and phenotypic analyses of six offspring of a genome-edited hornless bull

2019 ◽  
Vol 38 (2) ◽  
pp. 225-232 ◽  
Author(s):  
Amy E. Young ◽  
Tamer A. Mansour ◽  
Bret R. McNabb ◽  
Joseph R. Owen ◽  
Josephine F. Trott ◽  
...  
Keyword(s):  
Donor Plasmid ◽  
Genomic Alterations ◽  
Bioinformatics Analyses ◽  
The Public ◽  
Homology Directed Repair ◽  
Naturally Occurring ◽  
A Genome ◽  
Dairy Bulls ◽  
Illumina Hiseq4000 ◽  
Dairy Bull

Abstract Genome editing followed by reproductive cloning was previously used to produce two hornless dairy bulls. We crossed one genome-edited dairy bull, homozygous for the dominant PC Celtic POLLED allele, with horned cows (pp) and obtained six heterozygous (PCp) polled calves. The calves had no horns and were otherwise healthy and phenotypically unremarkable. We conducted whole-genome sequencing of all animals using an Illumina HiSeq4000 to achieve ~20× coverage. Bioinformatics analyses revealed the bull was a compound heterozygote, carrying one naturally occurring PC Celtic POLLED allele and an allele containing an additional introgression of the homology-directed repair donor plasmid along with the PC Celtic allele. These alleles segregated in the offspring of this bull, and inheritance of either allele produced polled calves. No other unintended genomic alterations were observed. These data can be used to inform conversations in the scientific community, with regulatory authorities and with the public around ‘intentional genomic alterations’ and future regulatory actions regarding genome-edited animals.

scholarly journals Reimagining data responsibility: 10 new approaches toward a culture of trust in re-using data to address critical public needs

Data & Policy ◽  
2021 ◽  
Vol 3 ◽  
Author(s):  
Stefaan G. Verhulst
Keyword(s):  
Data Science ◽  
Open Data ◽  
The Internet ◽  
Policy Makers ◽  
The Public ◽  
The World ◽  
Comprehensive Survey ◽  
Data Stewardship ◽  
Using Data ◽  
The Internet Of Things

Abstract Data and data science offer tremendous potential to address some of our most intractable public problems (including the Covid-19 pandemic). At the same time, recent years have shown some of the risks of existing and emerging technologies. An updated framework is required to balance potential and risk, and to ensure that data is used responsibly. Data responsibility is not itself a new concept. However, amid a rapidly changing technology landscape, it has become increasingly clear that the concept may need updating, in order to keep up with new trends such as big data, open data, the Internet of things, and artificial intelligence, and machine learning. This paper seeks to outline 10 approaches and innovations for data responsibility in the 21st century. The 10 emerging concepts we have identified include: End-to-end data responsibility Decision provenance Professionalizing data stewardship From data science to question science Contextual consent Responsibility by design Data asymmetries and data collaboratives Personally identifiable inference Group privacy Data assemblies Each of these is described at greater length in the paper, and illustrated with examples from around the world. Put together, they add up to a framework or outline for policy makers, scholars, and activists who seek to harness the potential of data to solve complex social problems and advance the public good. Needless to say, the 10 approaches outlined here represent just a start. We envision this paper more as an exercise in agenda-setting than a comprehensive survey.

Evaluation of the impact of public investment programs on social and economic performance of territories

2019 ◽  
pp. 109-123
Author(s):  
I. E. Limonov ◽  
M. V. Nesena
Keyword(s):  
Bayesian Modeling ◽  
Positive Impact ◽  
Public Investment ◽  
Difference In Differences ◽  
The Public ◽  
Export Activity ◽  
Regional Investment ◽  
The Difference ◽  
The Impact ◽  
Development Institution

The purpose of this study is to evaluate the impact of public investment programs on the socio-economic development of territories. As a case, the federal target programs for the development of regions and investment programs of the financial development institution — Vnesheconombank, designed to solve the problems of regional development are considered. The impact of the public interventions were evaluated by the “difference in differences” method using Bayesian modeling. The results of the evaluation suggest the positive impact of federal target programs on the total factor productivity of regions and on innovation; and that regional investment programs of Vnesheconombank are improving the export activity. All of the investments considered are likely to have contributed to the reduction of unemployment, but their implementation has been accompanied by an increase in social inequality.

The Impact of the Public Expenditure Cuts Policy on the Labor Market in Vietnam

2012 ◽  
pp. 22-46
Author(s):  
Huong Nguyen Thi Lan ◽  
Toan Pham Ngoc
Keyword(s):  
Labor Market ◽  
Computable General Equilibrium ◽  
Impact Evaluation ◽  
Public Expenditure ◽  
Computable General Equilibrium Model ◽  
Living Standard ◽  
Input Output ◽  
Policy Makers ◽  
The Public ◽  
The Impact

The purpose of this study is to evaluate the impact of public expenditure cuts on employment and income to support policies for the development of the labor mar- ket. Impact evaluation is of interest for policy makers as well as researchers. This paper presents a method – that is based on a Computable General Equilibrium model – to analyse the impact of the public expenditure cuts policy on employment and income in industries and occupations in Vietnam using macro data, the Input output table, 2006, 2008 and the 2010 Vietnam Household Living Standard Survey.

A mechanism to improve the allocation of public investment funds

2020 ◽  
Vol 26 (7) ◽  
pp. 1522-1533
Author(s):  
A.V. Larionov
Keyword(s):  
Panel Data ◽  
Random Effects ◽  
Public Investment ◽  
Allocative Efficiency ◽  
Investment Funds ◽  
The Public ◽  
Panel Data Regression ◽  
Data Regression ◽  
Efficiency And Effectiveness ◽  
Effective System

Subject. This article deals with the issue of improving the public investment allocative efficiency. Objectives. The article aims to develop an approach to improve the efficiency and effectiveness of public investment in the economy. Methods. The study is based on a panel data regression with random effects. Conclusions and Relevance. All sectors of the economy have different demand for investment resources attracted, determined by operational and technological aspects. The results of the study can be used to develop an effective system of public investment.

Penguatan Wilayah Perbatasan: Studi Kasus Pulau Sebatik Kabupaten Nunukan Provinsi Kalimantan Timur

2015 ◽  
Vol 12 (2) ◽  
pp. 110
Author(s):  
Baskoro Wicaksono
Keyword(s):  
Central Government ◽  
Secondary Data ◽  
Well Being ◽  
Research Strategy ◽  
Management Policy ◽  
Infrastructure Development ◽  
Policy Makers ◽  
The Public ◽  
East Kalimantan ◽  
Border Policy

This study describes the border management policy conducted by the central government, provinceof East Kalimantan and Nunukan. Policies such as the establishment of regulatory, institutionalstrengthening, programs and infrastructure development. The policy is getting good responsefrom the elite and the masses. On the other hand policy makers have expectations of localcommunities border synergism Sebatik Island in order to build and develop the border areas so asto break the chain of dependence on Malaysia. The research was conducted in Sebatik Island,East Kalimantan province Nunukan with the formulation of the problem (a) what policies areoriented to maintain borders, (b) How is the public response to government policy, (c) What areyour expectations of policy makers in local communities to regional border. This study usedqualitative methods to phenomenological research strategy. Techniques of data collection in thisstudy using two ways, namely in-depth interviews and secondary data view Results indicate thatthe existing policy of both the central and provincial to district borders do not solve the problembecause it is made on the island of Sebatik with other border regions. The policy does not includelocal knowledge, where it is desperately needed by the people Sebatik. In addition to policies onprograms and infrastructure development of the center, the district adopted a policy of inactionagainst the illegal cross-border trade, which on the one hand against the rules but if enforced thenpeople can not perform economic activities that impact well-being. Policies like this gets a positiveresponse from the public. Expectations for the future border policy is to load local content orlocal knowledge.Keyword: border policy, local knowledge, dependent relationship

Partisipasi Masyarakat dalam Proses Kebijakan Daerah

2012 ◽  
Vol 10 (2) ◽  
pp. 163-177
Author(s):  
Abdullah Manshur
Keyword(s):  
Public Policy ◽  
Public Participation ◽  
Policy Evaluation ◽  
Policy Process ◽  
Policy Formulation ◽  
Problem Situation ◽  
Policy Cycle ◽  
Policy Makers ◽  
The Public ◽  
Policy Making Process

Public policy is a decision to deal with a particular problem situation, that identifies the objectives, principles, ways, and means to achieve them. The ability and understanding of policy makers in the policy-making process is very important for the realization of public policy of rapid, accurate and adequate. The product to suit the needs of the public policy, public participation in the policy process is needed in the policy cycle, from policy formulation to policy evaluation. This paper attempts to review the importance of community participation and other forms of public participation in the policy process, in particular, policy areas.

scholarly journals Anxiety Level of University Students During COVID-19 in Saudi Arabia

2020 ◽  
Vol 11 ◽  
Author(s):  
Heba Bakr Khoshaim ◽  
Areej Al-Sukayt ◽  
Karuthan Chinna ◽  
Mohammad Nurunnabi ◽  
Sheela Sundarasen ◽  
...  
Keyword(s):  
Saudi Arabia ◽  
University Students ◽  
Anxiety Level ◽  
Educational Systems ◽  
Policy Makers ◽  
Level Of Education ◽  
Future Studies ◽  
The Public ◽  
Public Enemy ◽  
Anxiety Levels

COVID-19 is the worst pandemic of this millennium, and it is considered to be the “public enemy number one.” This catastrophe has changed the way we live in the blink of an eye. Not only has it threatened our existence and health status, but the damage associated with it could equally affect our economic, social, and educational systems. The focus of this study was on the anxiety level of university students during the COVID-19 pandemic in Saudi Arabia. The study was conducted between March and June 2020. A questionnaire was administered online, and 400 completed questionnaires were returned. In this study, the Zung self-rating anxiety scale was used to determine the anxiety levels among the respondents. The results indicated that about 35% of the students experienced moderate to extreme levels of anxiety. Anxiety was highly associated with age, sex, and level of education. These findings can enlighten government agencies and policy makers on the importance of making prompt, effective decisions to address students' anxiety during the COVID-19 pandemic. Researchers are encouraged to focus their future studies on how to develop strategies to boost students' resilience and enhance their adaptability skills for similar disasters in the future.

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