Comparison of antioxidant activity and tyrosinase inhibition in fresh and processed white radish, garlic and ginger

2015 ◽  
Vol 9 (3) ◽  
pp. 369-374 ◽  
Author(s):  
Apisit Somman ◽  
Napa Siwarungson
Keyword(s):  
Antioxidant Activity ◽  
Tyrosinase Inhibition ◽  
White Radish

scholarly journals Tyrosinase Inhibition, Antiglycation, and Antioxidant Activity of Xylocarpus granatum

2020 ◽  
Vol 12 (1) ◽  
pp. 70-75
Author(s):  
Irmanida Batubara ◽  
Maily Mustofa ◽  
Wulan Tri Wahyuni ◽  
Kilala Tilaar ◽  
Waras Nurcholis ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethanolic Extract ◽  
Stem Bark ◽  
Tyrosinase Inhibitor ◽  
Tyrosinase Inhibition ◽  
Fruit Peel ◽  
Vitro Assay ◽  
Fruit Flesh ◽  
Xylocarpus Granatum ◽  
Stem Extract

Xylocarpus granatum is mangrove plant that traditionally used as face powder in Central Sulawesi, Indonesia which related to antioxidant, antiglycation and tyrosinase inhibition activities. This study aimed to evaluate the potency of X. granatum as a tyrosinase inhibitor, antiglycation, and antioxidant. The leaves, stem, stem bark, fruit flesh, fruit peel, and kernel of X. granatum were extracted using ethanol then their tyrosinase inhibition, antiglycation, and antioxidant were evaluated. Tyrosinase inhibition activity was evaluated using in vitro assay with L-tyrosine and L-DOPA as the substrate of monophenolase and diphenolase. Antiglycation activity was studied by measuring the excitation and emission fluorescence from glucose and fructose reaction with Bovine Serum Albumin. Antioxidant activity was measured using the DPPH radical scavenging assay. The result showed that the ethanolic extract of fruit flesh has higher potency as tyrosinase inhibitor (IC50 of 393.8 mg/L and IC50 of 448 mg/L, respectively for monophenolase and diphenolase). Antiglycation assay showed that the ethanolic extract of stem bark provides the strongest antiglycation activity with an IC50 of 118.1 mg/L. Meanwhile, fruit peel provides the strongest antioxidant activity with an IC50 of 5.5 mg/L. Fractionation of ethanolic extracts of each part of X. granatum tree yield fractions with lower bioactivity compared to the crude extract. Moreover, stem extract and fractions from two different locations (Tarakan and Kendari) tend to have different bioactivities strengths.  The stem part of X granatum could be developed as new raw material of cosmetic product in Indonesia, while ethanol as the solvent for extraction, and the different bioactivity of stem extract from different location can be the consideration for the industry to standardize the extract prior to production of final product.

scholarly journals Screening and Bioguided Fractionation of Mimosa pigra L. Bee Pollen with Antioxidant and Anti-Tyrosinase Activities

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Phanthiwa Khongkarat ◽  
Rico Ramadhan ◽  
Preecha Phuwapraisirisan ◽  
Chanpen Chanchao
Keyword(s):  
Antioxidant Activity ◽  
Silica Gel ◽  
Column Chromatography ◽  
Size Exclusion ◽  
Tyrosinase Inhibition ◽  
Chiang Mai ◽  
Bee Pollen ◽  
Mimosa Pigra ◽  
Exclusion Chromatography ◽  
Chiang Mai Province

AbstractBee pollen, one of the economic bee products, is widely known as a nutritional food with many bioactivities that depend on many such factors as bee species, plant source and biogeography. For this study, bee pollen was collected from Apis mellifera, harvested from the flowers of mimosa (Mimosa pigra L.) in the Chiang Mai province, Thailand. The sample was extracted in methanol (MeOH) and then sequentially partitioned with hexane, dichloromethane (DCM) and MeOH in order to isolate compounds depending on their polarities. The obtained extracts were then tested for their antioxidant and anti-tyrosinase activities through 1,1-diphyenyl-2-picrylhydrazyl (DPPH) assay and for/through inhibition of mushroom tyrosinase extract, respectively. The DCM partitioned extract of mimosa flower bee pollen (DCMMBP) provided the highest antioxidant activity, with an effective concentration at 50% (EC50) of 192.1 μg/mL, and was further fractionated by silica gel 60 column chromatography and Sephadex LH20 size exclusion chromatography. All fractions were tested for their antioxidant activity and analyzed for the chemical structure through nuclear magnetic resonance (NMR). The most active fraction (EC50 of 121.3 μg/mL) was a mixture of compounds, but the isolated flavonoid, naringenin, had a negligible antioxidant activity. In contrast, all three partitioned extracts and pooled fractions after silica gel 60 column chromatography provided no anti-tyrosinase activity (IC50 of > 500 μg/mL) and a very low percentage of tyrosinase inhibition (0–2.99 ± 3.18%), compared to kojic acid (IC50 of 8.58 μg/mL and tyrosinase inhibition of 74.2 ± 1.25%).

scholarly journals USING CELLULASE TO IMPROVE THE EXTRACTION EFFICIENCY OF THE ANTIOXIDANT COMPOUNDS FROM WHITE RADISH (Raphanus sativus L.) POWDER

2018 ◽  
Vol 54 (4A) ◽  
pp. 48
Author(s):  
Pham Thi Kim Ngoc
Keyword(s):  
Antioxidant Activity ◽  
Raphanus Sativus ◽  
Control Sample ◽  
Enzyme Concentration ◽  
Antioxidant Compounds ◽  
Extraction Temperature ◽  
Assisted Extraction ◽  
Raphanus Sativus L ◽  
Electron Microscopy Analysis ◽  
White Radish

White radish (Raphanus sativus L.) is an edible root vegetable of the Brassicaceae family. It is cultivated and consumed all over the world. Nowadays, radishes are used and eaten by many people in their daily meals. Some studies showed that it has a high content of antioxidant compounds. In this study, we used enzyme - assisted extraction (EAE) to produce an antioxidant - rich extract. The antioxidant activity of the extract was determined by the ABTS radical cation scavenging capacity. The optimal extraction conditions of the antioxidant compounds from white radish were: enzyme concentration of 1.75 % (v/w), extraction time of 81 minutes, pH 4.56 and extraction temperature of 48 oC. As a result, the antioxidant activity of the extract was 0.1594 ± 0.033 m Moltrolox equivalent antioxidant capacity (TEAC)/g and 161 % higher than that of the control sample(CE).Furthermore, SEM (scanning electron microscopy) analysis result showed that there was a difference in the surface structure of white radish powder after being extracted by CE and EAE.

scholarly journals TOTAL PHENOLIC AND FLAVONOID CONTENT, FREE RADICAL SCAVENGING ACTIVITY AND TYROSINASE INHIBITION OF CORN COB (ZEA MAYS) EXTRACT

2021 ◽  
pp. 99-102
Author(s):  
INDRI KUSUMA DEWI ◽  
SUHENDRIYO ◽  
INDARTO ◽  
SUWIJIYO PRAMONO ◽  
ABDUL ROHMAN ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Zea Mays ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Total Phenolic ◽  
Corn Cob ◽  
Tyrosinase Inhibition ◽  
Flavonoid Content ◽  
Phenolic And Flavonoid

Objective: Corn cob is a part of plant Zea mays that have a phenolic and flavonoid compounds. This research aims to evaluate total phenolic and flavonoid content, free radical scavenging activity and tyrosinase inhibiton of corn cob (Zea mays) extract. Methods: Corn cob were determined in Poltekkes Kemenkes Surakarta. Meanwhile, corn cob were extracted using maceration method applying 70% ethanol solvent. This extract were analyzed through the total fenolic and flavonoids content tests apply spectrofotometric UV Vis, antioxidant activity using radical scavenging test 2,2-difenill-1-pikrilhidrazil (DPPH) and tyrosinase inhibition test applying in vitro tyrosinase enzyme inhibition. Results: Total phenolic content of the corn cob extract were 1.76 %b/b EAG, while the total of flavonoids content were 0.42%b/b EK. The antioxidant activity using DPPH method test of corn cob extract were values IC50 38.57 µg/ml. Tyrosinase inhibition of corn cob extract were values IC50 919.78µg/ml. Conclusion: Corn cob extract had antioxidant activity and tyrosinase inhibition.

scholarly journals ANTIOXIDANT ACTIVITY OF NUTRI-PEPPER ENHANCER

2021 ◽  
Vol 3 (1) ◽  
pp. 1-9
Author(s):  
Yap Ann ◽  
Sang Jam ◽  
Lawrance Tuah
Keyword(s):  
Antioxidant Activity ◽  
Xanthine Oxidase ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Piper Nigrum ◽  
Scavenging Activity ◽  
Inhibition Activity ◽  
Tyrosinase Inhibition ◽  
Superoxide Scavenging Activity

Introduction: Kacangma (Leonurus sibiricus), turmeric (Curcuma longa) and black pepper (Piper nigrum) are the oldest and most important spice crops used for culinary purposes in Malaysia for centuries. Although many biological activities including antioxidant properties (AP) have been reported, there are no confirmed reports on the activities of this cocktail, nutri-Pepper enhancer. Purpose: The study evaluated the AP of the Nutri-Pepper enhancer in vitro. Methodology: The AP were evaluated using in vitro antioxidant assays where the total polyphenolic content (TPC), DPPH radical scavenging activity, tyrosinase inhibition activity, xanthine oxidase superoxide scavenging activity (XOD), and oxygen radical absorbance capacity (ORAC) were measured.  Results: Based on the antioxidant activity range, extract of Nutri pepper enhancer was high in tyrosinase inhibition activity, 87.16%, moderate in xanthine oxidase superoxide scavenging activity (XOD) 69.72% and low in 1,2-diphenyl-2- picrylhydrazyl radical scavenging activity (DPPH) 46.78%. On the other hand, the extract showed high ORAC and total phenolic content with the value of 236,00 µmol TE/100g and 282 mg/100 GAE. Conclusion: This finding proven that the consumption of this product can reduce or even eliminate the harmful effects on humans from contaminants in foods and from the environment; this is even more important for people living in polluted cities.

scholarly journals Evaluation of Antioxidant Activity and Inhibition of Tyrosinase Activity of Raphanus sativus var. caudatus Alef Extract

2020 ◽  
Vol 17 (8) ◽  
pp. 838-850
Author(s):  
Panadda YONGPRADOEM ◽  
Natthida WEERAPREEYAKUL
Keyword(s):  
Antioxidant Activity ◽  
Caffeic Acid ◽  
Aqueous Extract ◽  
Raphanus Sativus ◽  
Protocatechuic Acid ◽  
Chemical Constituents ◽  
Radical Scavenging ◽  
Positive Control ◽  
Hydroxybenzoic Acid ◽  
Tyrosinase Inhibition

This study was aimed to determine antioxidant and tyrosinase inhibition effects of the pod of Raphanus sativus L. var. caudatus Alef extract. The compounds consisted in the extracts were identified by HPLC from standard peak comparison. Pod was extracted by using 2 different solvents-dichloromethane (DCM) and water. The antioxidant activity was evaluated based on free radical scavenging (DPPH) activity and ferric reducing ability (FRAP) assay. The lightening effect was determined from the inhibition of mushroom tyrosinase in vitro. The results showed that DCM extract contained sulforaphene, protocatechuic acid, p-hydroxybenzoic acid, caffeic acid, and ferulic acid.  The aqueous extract contained sulforaphene, protocatechuic acid, p-hydroxybenzoic acid, caffeic acid, vanillic acid, and p-coumaric acid.  The DPPH· scavenging effect expressed as the IC50 values for DCM and aqueous extracts were 883.38 ± 22.9 and 1,160.49 ± 22.30 µg/mL, respectively. The reducing power of aqueous extract (218.27 ± 0.010 µM FeSO4 equivalent) was greater than DCM extract (166.34 ± 0.018 µM FeSO4 equivalent) at the same concentration (2,000 µg/mL).  However, both extracts showed lesser antioxidant activity than gallic acid, a positive control. DCM extract showed higher tyrosinase inhibitory effect than aqueous extract but lesser than kojic acid, a positive control. Both extracts at 2,000 µg/mL concentration exerted 42 and 19 % tyrosinase inhibition, respectively. In conclusion, the extraction solvent yielded different chemical constituents and thus the activities. The DCM extract exerted greater antioxidant activity and tyrosinase inhibition activity than the aqueous extract. The extract fractionation is required to get higher yields of bioactive compounds prior to further study and for cosmetics product development.

scholarly journals Food-Derived Bioactive Peptides with Antioxidative Capacity, Xanthine Oxidase and Tyrosinase Inhibitory Activity

Processes ◽  
2021 ◽  
Vol 9 (5) ◽  
pp. 747
Author(s):  
Anthony Thaha ◽  
Bor-Sen Wang ◽  
Yu-Wei Chang ◽  
Shih-Min Hsia ◽  
Tsui-Chin Huang ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Xanthine Oxidase ◽  
Milk Fat ◽  
Bioactive Peptides ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Egg White ◽  
Food Protein ◽  
Antioxidative Capacity ◽  
Tyrosinase Inhibition

Bioactive peptides (BPs) released by proteases from different food protein sources are often served as antioxidants in food applications. This study aims to investigate 11 BPs derived from fish and egg white as potential natural antioxidants by antioxidant activity assays. The kinetic activity of the BPs against xanthine oxidase (XOD) and tyrosinase was also analyzed. The antioxidative capacity of the BPs indicated that VWWW (VW4, mackerel meat), followed by IRW (IW3, egg white) and VKAGFAWTANQQLS (VS14, tuna backbone protein), possessed the highest antioxidant activity in 1,1-diphenyl-2-picrylhydrazyl radical (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) and reducing power (RP) assays. Both the free-radical scavenging score predicted from the AnOxPePred algorithm and the DPPH, ABTS and RP results indicated that VW4 was the best antioxidant. Furthermore, the XOD and tyrosinase inhibition by three selected peptides exhibited competitive patterns of effective inhibition. The half maximal inhibitory concentrations (IC50) of the peptides for XOD inhibition were 5.310, 3.935, and 1.804 mM for VW4, IW3, and VS14, respectively, and they could serve as competitive natural XOD inhibitors. The IC50 of the peptides for tyrosinase inhibition were 1.254, 2.895, and 0.595 mM for VW4, IW3, and VS14, respectively. Overall, VW4, IW3, and VS14 are potential antioxidants and natural XOD inhibitors for preventing milk-fat oxidation, and anti-browning sources for inhibiting food-derived tyrosinase oxidation.

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