Anticancer Activity of Aqueous Fenugreek Seed Extract Against Pancreatic Cancer, Histological Evidence

Objective: In recent years, herbal plants have been got more attention due to their diverse presence of phytochemicals and its biological properties. Hepatocellular carcinoma is one of the major worldwide problems primarily caused by hepatitis B and C virus infection. End-stage liver cancer treatment options are limited, thus requiring expensive liver transplantation which is not available in many countries.Methods: In the present study, the Mucuna pruriens seed extract was analyzed for phytochemicals, antioxidant activity, total phenols, and total flavonoids content. The seed extract was further analyzed for its anticancer activity by culturing liver cancer cell line. The above protocols were done by standard methods.Results: The seed extract of M. pruriens revealed more number of phytochemicals in different organic solvents. 1,1- Diphenyl- 2- picrylhydrozyl scavenging activity of plant extract was more in ethanol extract (98.051±0.547) among all other solvents. The total phenols and flavonoids content in ethanol extract were 46.442±0.353 mg gallic acid equivalent/g and 2.254±0.647 mg - quercetin equivalent /g, respectively. IC50 value of 3-(4, 5-dimethythiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) analysis of Hep-2 cell line was 150 (μg/ml).Conclusion: The present study revealed about the phytochemical contents and antioxidant potential of M. pruriens seeds. Further, the MTT analysis proved that the seed extract was effective against cancer cells and also used to treat many diseases.

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Comparison of Antibacterial Efficacy of Fenugreek Seed Extract Rinse and Nigella Sativa Seed Extract Rinse against Streptococcus Mutant Colonies

Journal of Pharmaceutical Research International ◽

10.9734/jpri/2021/v33i50b33430 ◽

2021 ◽

pp. 79-86

Author(s):

Nasima Iqbal ◽

ATA UR Rehman ◽

Syeda Amber Zaidi ◽

Kiran Khan ◽

Lubna Farooq ◽

...

Keyword(s):

Nigella Sativa ◽

Positive Control ◽

Seed Extract ◽

Control Group ◽

Distilled Water ◽

Post Hoc Analysis ◽

Fenugreek Seed ◽

Study Participants ◽

Post Hoc ◽

Nigella Sativa Seed

Background: Dental infections were caused by the bacterium overgrowth on the surface of the tooth, and treatment should always be set up to prevent this development.Antibiotics have long been used as a conventional antibacterial medication, but their overuse has resulted in microbes gaining resistance to many of the antibiotics, trying to make many commercialized therapeutic remedies ineffectual and resulting to infection recurrence. In this regard we aim to analyze the antibacterial activity of nigella sativa seeds’ and fenugreek seed extract rinses against S. mutans' colonies. Methodology: It was a preclinical experimental study conducted at Baqai Medical and Dental College Karachi., from January - June 2021. Calculated sample size was n = 80 Consecutive sampling technique was used. Plaque of study participants was collected on sterile strips that was transported to laboratory for culture in sterile containers.The extract of Fenugreek seed and nigella sativa seed was kept in an airtight bottle and stored in a refrigerator till usage. The extracts were diluted in distilled water in 1:4 (Extract: Distilled water). Study participants were instructed to not brush their teeth before sampling. Study participants were divided into four groups (negative control, positive control, fenugreek seed extract group and Nigella sativa group) each group had 20 participants. Diluted Fenugreek seed extract and Nigella sativa extract was given to experimental groups for rinses. Results: There was significant decrease in number of colonies in positive controls, fenugreek seed extract group and nigella sativa extract group and there was no change in number of colonies in control group. Furthermore, the analysis showed significant (p-value = 0.001) difference among the groups followed by post hoc analysis. Post hoc analysis showed no difference between positive control, fenugreek seed extract group and nigella sativa group. Conclusion: Fenugreek seed extract and Nigella Sativa seed extract showed comparable antibacterial properties. Also, the effect was found to be similar to commercially available mouth rinse.

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Fenugreek Seed Extract Enhanced the Growth of Vicia faba and Zea mays Seedlings

Egyptian Journal of Botany ◽

10.21608/ejbo.2017.777.1047 ◽

2017 ◽

Vol 57 (2) ◽

pp. 363-377 ◽

Cited By ~ 1

Author(s):

Mahmoud Madany ◽

Radwan Khalil

Keyword(s):

Zea Mays ◽

Vicia Faba ◽

Seed Extract ◽

Fenugreek Seed

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Development of Cookies Using Fenugreek Seed Extract as a Functional Ingredient

International Journal of Food Nutrition and Dietetics ◽

10.21088/ijfnd.2322.0775.3115.4 ◽

2015 ◽

Vol 3 (1) ◽

pp. 23-28

Author(s):

Sonu . ◽

◽

S. A. Wani ◽

Pradyuman Kumar ◽

◽

...

Keyword(s):

Seed Extract ◽

Functional Ingredient ◽

Fenugreek Seed

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Biosynthesis of Bonelike Apatite 2D Nanoplate Structures Using Fenugreek Seed Extract

Nanomaterials ◽

10.3390/nano10050919 ◽

2020 ◽

Vol 10 (5) ◽

pp. 919

Author(s):

Abdalla Abdal-hay ◽

H. Fouad ◽

Basheer A. ALshammari ◽

Khalil Abdelrazek Khalil

Keyword(s):

Inductively Coupled Plasma ◽

Control Sample ◽

Cost Effective ◽

Atomic Emission ◽

Chemical Precipitation ◽

Seed Extract ◽

Hydroxyl Groups ◽

Average Width ◽

Fenugreek Seed ◽

Bonelike Apatite

An innovative, biomimetic, green synthesis approach was exploited for the synthesis of humane and environmental friendly nanomaterials for biomedical applications. Ultrafine bonelike apatite (BAp) 2D plate-like structures were prepared using fenugreek seed extract during the biosynthesis wet-chemical precipitation route. The chemical analysis, morphology and structure of the prepared 2D nanoplates were characterized by inductively coupled plasma atomic emission spectroscopy (ICP-OES), electron microscopy (SEM and TEM), X-ray diffraction (XRD) and Fourier transform infrared (FTIR) spectroscopy. A 2D plate-like nanostructure of BAp with an average width (length) of 12.67 ± 2 nm and thickness of 3.8 ± 1.2 nm was obtained. BAp 2D crystals were tuned by interaction with the fenugreek organic molecules during the fabrication process. In addition to Ca and P ions, bone mineral sources such as K, Mg, Na, SO4 and CO3 ions were incorporated into BAp nanoplates using fenugreek seed extract. The overall organic molecule concentration in the reaction process increased the effectiveness of hydroxyl groups as nucleation sites for BAp crystals. Accordingly, the size of the biosynthesized BAp plate-like structure was reduced to its lowest value. Biosynthesis BAp 2D plate-like nanocrystals showed good viability and higher growth of MC3T3 osteoblast-like structures than that of the control sample. BAp 2D nanoplates prepared by a facile, ecofriendly and cost-effective approach could be considered a favorable osteoconductive inorganic biomaterial for bone regeneration applications.

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