Evaluation of intraepithelial lymphocytes, goblet cells and immunoglobulin genes in the intestinal mucosal tissue of Pelodiscus sinensis after challenge with Aeromonas veronii bv. sobria and lipopolysaccharide

2018 ◽  
Vol 85 (1) ◽  
pp. 177-185 ◽  
Author(s):  
Jiehao Xu ◽  
Hanxiang Chen ◽  
Wanrong Zhang ◽  
Haisheng Xu
Keyword(s):  
Goblet Cells ◽  
Intraepithelial Lymphocytes ◽  
Immunoglobulin Genes ◽  
Mucosal Tissue ◽  
Pelodiscus Sinensis ◽  
Aeromonas Veronii

Effect of fermented whey with a probiotic bacterium on gut immune system

2020 ◽  
Vol 87 (1) ◽  
pp. 134-137 ◽  
Author(s):  
Gisela García ◽  
María Emilia Agosto ◽  
Lilia Cavaglieri ◽  
Cecilia Dogi
Keyword(s):  
Immune System ◽  
Lactobacillus Rhamnosus ◽  
Goblet Cells ◽  
Intraepithelial Lymphocytes ◽  
Probiotic Bacterium ◽  
Control Group ◽  
Mice Model ◽  
Gut Immune System ◽  
Phosphate Buffered Saline

AbstractThe aim of the work presented in this Research Communication was to evaluate the effect of fermented whey (FW) with Lactobacillus rhamnosus RC007 in a mice model. BALB/c mice were divided into three groups: control group: animals received orally 0.1 ml of phosphate buffered saline (PBS); FW group: animals received orally 0.1 ml of FW; whey (W) group: animals received orally 0.1 ml of W without fermentation with probiotic bacterium. After 10 d mice were sacrificed. Small intestines were collected for determination of IL-10; IL-6, TNFα, goblet cells and intraepithelial lymphocytes. Increases of all the cytokines assayed were observed in mice that received FW compared to control and W group. The ratio between the anti and pro-inflammatory cytokines (IL-10/TNFα) increased in the group of mice that received FW. The number of goblet cells and intraepithelial lymphocytes were also increased in animals that received FW. The results showed that FW with L. rhamnosus RC007 was able to stimulate and to modulate mouse immune system. Whey fermented by this probiotic bacterium is an interesting alternative for development of a new food additive for pig production, taking advantage of the beneficial properties of probiotic bacterium and the nutritional properties of whey.

Mucosal lesions in the rat associated with the attachment ofMoniliformis(Acanthocephala)

1983 ◽  
Vol 57 (2) ◽  
pp. 85-90 ◽  
Author(s):  
Jean Martin ◽  
D. W. T. Crompton ◽  
Aruna Singhvi
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Goblet Cells ◽  
Intestinal Wall ◽  
Anterior Region ◽  
Mucosal Tissue ◽  
Mucosal Lesions ◽  
Scanning Electron

ABSTRACTBy means of scanning electron microscopy, aspects of the attachment ofMoniliformis dubius(Acanthocephala) to the intestinal wall of the rat are described together with various changes in the structure and form of the mucosa. Attachment of the parasite results in the formation of a distinct pit around the everted proboscis and a crater around the neck. Contact between the anterior region of the trunk of the worm and the intestine of the host appears to lead to villous fusion so that characteristic grooves are formed in the mucosal tissue. Evidence was also obtained to suggest that host goblet cells may become exhausted during the course of an infection withMoniliformis.

scholarly journals Morpho-histochemistry of the distal intestines and rectum of African catfish (Clarias gariepinus)

2014 ◽  
Vol 59 (3) ◽  
pp. 287-300
Author(s):  
Ekele Ikpegbu ◽  
Uchenna Nlebedum ◽  
Chikera Ibe
Keyword(s):  
Clarias Gariepinus ◽  
Smooth Muscles ◽  
Goblet Cells ◽  
Intraepithelial Lymphocytes ◽  
Muscle Fibres ◽  
African Catfish ◽  
Tissue Slices ◽  
Tunica Muscularis ◽  
Acid Mucins ◽  
Columnar Cells

The morphology of the farmed African catfish distal intestines and rectum was investigated. The samples were dissected out and the tissue slices were passed through graded ethanol, cleared in xylene, embedded in paraffin wax, sectioned and stained for light microscopy. Grossly, the intestine was subdivided into proximal, middle, distal and rectal regions based on palpable thickness and diameter of the regions. No intestino-rectal valve was observed; hence the rectum was not well defined. The histology revealed the presence of tunica mucosa of simple columnar cells, lamina propria of collagen fibres and muscularis mucosae of smooth muscle fibres; submucosa of loose connective tissues and blood vessels; tunica muscularis of smooth muscles in an inner circular and outer longitudinal arrangement; and a serosa in all regions except the rectum and anus that presented tunica adventitia. The mucosal folds were fingerlike to orange leaf shaped ingrowths into the lumen on a transverse section. They decreased in complexity and height but increased in width towards the rectum. The lining epithelium of the intestinal tract was comprised of the absorptive simple columnar cells containing goblet cells and intraepithelial lymphocytes. Some rectal simple columnar epithelium contained supranuclear vacuoles while others contained non-vacuolated cytoplasm. The goblet cells contained neutral and acid mucins, and increased in number progressively towards the rectum. The anal tunica muscularis contained mostly skeletal muscles.

scholarly journals Intraepithelial lymphocytes, goblet cells and VIP-IR submucosal neurons of jejunum rats infected with Toxoplasma gondii

2012 ◽  
Vol 93 (4) ◽  
pp. 279-286 ◽  
Author(s):  
Débora M. G. Sant’Ana ◽  
Marcelo B. Góis ◽  
Jacqueline N. Zanoni ◽  
Aristeu V. da Silva ◽  
Cleverton J. T. da Silva ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Goblet Cells ◽  
Intraepithelial Lymphocytes

Toxoplasma gondiipromotes changes in VIPergic submucosal neurons, mucosal intraepithelial lymphocytes, and goblet cells during acute infection in the ileum of rats

2017 ◽  
Vol 30 (5) ◽  
pp. e13264 ◽  
Author(s):  
L. C. L. Schneider ◽  
J. C. P. do Nascimento ◽  
A. R. Trevizan ◽  
M. B. Góis ◽  
S. C. Borges ◽  
...  
Keyword(s):  
Acute Infection ◽  
Goblet Cells ◽  
Intraepithelial Lymphocytes

scholarly journals FREQUENCY AND DISTRIBUTION OF IMMUNOGLOBULIN CONTAINING PLASMA CELLS IN ILEUM REPRESENTING GUT-ASSOCIATED-LYMPHATIC TISSUES IN THE BCRDV-VACCINATED BROILER

2013 ◽  
Vol 10 (1-2) ◽  
pp. 15-20
Author(s):  
M. A. Masum ◽  
M. Z. I. Khan ◽  
M. N. H. Siddiqi ◽  
M. Nasrin ◽  
N. Sultana
Keyword(s):  
Lamina Propria ◽  
Broiler Chickens ◽  
Plasma Cells ◽  
Goblet Cells ◽  
Intraepithelial Lymphocytes ◽  
Lymphatic Tissue ◽  
Histological Studies ◽  
The Core ◽  
And Control ◽  
Columnar Cells

Histological studies and development of immunoglobulin containing plasma cells in ileum representing gut-associated-lymphatic tissue of various group of broiler chickens vaccinated with Baby Chick Ranikhet Disease Vaccine (BCRDV) was carried out in the Department of Anatomy and Histology, Bangladesh Agricultural University, Mymensingh during January to October, 2011. A total of 24 day-old broiler chickens of both sexes were divided into two groups such as vaccinated and control. Vaccinated group of broiler chickens were vaccinated at third day through ocular route. It was observed that the villi of ileum were long and slender, lined by simple columnar cells with numerous goblet cells. Scattered lymphocytes and unorganized lymphatic nodules were present in the lamina propria and core of the villi. The frequency and distribution of lymphocytes in the lamina propria and intraepithelial lymphocytes were increased with concentration in vaccinated group. The length and width of mucosa were highest at D28. In the ileum of chickens, the IgA, IgG and IgM positive cells were found in the lamina propria, around the intestinal glands and in the core of the villi of both control and vaccinated group of chickens. The IgA positive cells were significantly (p<0.01) higher than IgG and IgM positive cells in all stages of study in each group of chickens.DOI: http://dx.doi.org/10.3329/bjvm.v10i1-2.15641

scholarly journals Cellular elements in the developing caecum of Japanese quail (Coturnix coturnix japonica): morphological, morphometrical, immunohistochemical and electron-microscopic studies

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Aalaa M. AbuAli ◽  
Doaa M. Mokhtar ◽  
Reda A. Ali ◽  
Ekbal T. Wassif ◽  
K. E. H Abdalla
Keyword(s):  
Japanese Quail ◽  
Immune Cells ◽  
Proximal Part ◽  
Goblet Cells ◽  
Intraepithelial Lymphocytes ◽  
Surface Epithelium ◽  
Electron Microscopic ◽  
Microscopic Studies ◽  
Immunological Surveillance ◽  
A Site

Abstract The present study aims to investigate the histological, histochemical and electron microscopic changes of the caecal proximal part of Japanese quail during both pre- and post-hatching periods starting from the 2nd embryonic day (ED) until four weeks post-hatching. On the 2nd and 3rd ED, the primordia of caeca appeared as bilateral swelling on the wall of the hindgut. On the 7th ED, the lamina propria/submucosa contained the primordia of glands. On the 8th ED, rodlet cells could be observed amongst the epithelial cells. On the 9th ED, the caeca began to divide into three parts with more developed layers. With age, the height and number of villi increased. On the 13th ED, immature microfold cells (M-cells) could be identified between the surface epithelium of the villi. The caecal tonsils (CTs) appeared in the form of aggregations of lymphocytes, macrophages, dendritic cells and different types of leukocytes. Telocytes and crypts of Lieberkuhn were observed at this age. On hatching day, the crypts of Lieberkuhn were well-defined and formed of low columnar epithelium, goblet cells, and enteroendocrine cells. Post-hatching, the lumen was filled with villi that exhibited two forms: (1) tongue-shaped villi with tonsils and (2) finger-shaped ones without tonsils. The villi lining epithelium contained simple columnar cells with microvilli that were dispersed with many goblet cells, in addition to the presence of a high number of intra-epithelial lymphocytes and basophils. Moreover, the submucosa was infiltrated by numerous immune cells. CD3 immunomarker was expressed in intraepithelial lymphocytes, while CD20 immunomarker showed focal positivity in CTs. In conclusion, the caecal immune structures of quails at post-hatching were more developed than those in pre-hatching life. The high frequency of immune cells suggests that this proximal part may be a site for immunological surveillance in the quail caecum. The cellular organisation of the caecum and its relation to the immunity was discussed.

scholarly journals Effect of Probiotics and Herbal Products on Intestinal Histomorphological and Immunological Development in Piglets

2020 ◽  
Vol 2020 ◽  
pp. 1-14
Author(s):  
Daiga Gāliņa ◽  
Līga Ansonska ◽  
Anda Valdovska
Keyword(s):  
Basal Diet ◽  
Goblet Cells ◽  
Inhibitory Effect ◽  
Intraepithelial Lymphocytes ◽  
Villus Height ◽  
Dual Effect ◽  
Ki 67 ◽  
Herbal Products ◽  
Colon Crypt ◽  
Experimental Group

The aim of the study was to evaluate the effect of probiotics and herbal products on the intestinal histomorphological and immunological development in piglets. Accordingly, 2-week-old piglets were allocated in 4 groups: C (basal diet), Pro (basal diet + probiotics), Pro+B (basal diet + probiotics + buckwheat bran), and H (powder of herbs). After 6 weeks of the experiment, 4 piglets from each experimental group were randomly selected and slaughtered at a slaughterhouse. Samples of tissue and digestive content from the jejunum and colon were collected for bacteriological, histological, and immunohistochemical examination. The results showed that probiotics increased the number of Lactobacillus spp. in the small p<0.05 and large intestines. The intestinal histomorphology was improved p<0.05 in all experimental groups by an increased villus height, VH : CD ration, colon crypt depth, and number of Ki-67+ epithelial cells. A higher number p<0.05 of goblet cells and their acidification were observed in group Pro, while the density of goblet cells was decreased by the herbs. Probiotics increased p<0.05 the number of intraepithelial lymphocytes (IELs), density of CD3+ cells in Peyer’s patches (PPs), and lamina propria (LP). In group H, a dual effect on the CD3+ cell distribution was observed. The herbs reduced p<0.05 the number of IELs and CD3+ in LP but increased the distribution of CD3+ cells in PPs. In the colon, herbs increased CD3+ cells in LP as well. It suggests that probiotics and herbs had influence on the intestinal histomorphology and the ability to modulate the mucosal immune system; however, the combination of probiotics and buckwheat bran was not so convincing, probably due to the inhibitory effect of the buckwheat bran on the probiotics used.

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