Effect of abiotic stress conditions on expression of the Lactobacillus brevis IOEB 9809 tyrosine decarboxylase and agmatine deiminase genes

Cytoplasmic streaming is one among the vital activities of the living cells. In plants cytolplasmic streaming could clearly be seen in hypocotyls of growing seedlings. To observe cytoplsmic streaming and its correlated intracellular trafficking an investigation was conducted in legumes in comparison with GFP-AtRab75 and 35S::GFP:δTIP tonoplast fusion protein expressing arabidopsis lines. These seedlings were observed under confocal microscopy with different buffer incubation treatments and under different stress conditions. GFP expressing 35S::GFP:δTIP tonoplast lines were looking similar to the control lines and differ under stress conditions. Movement of cytoplasmic invaginations within the tonoplast and cytoplasmic sub vesicle or bulb budding during cytoplasmic streaming was observed in hypocotyls of At-GFP tonoplast plants. We found the cytoplasmic bulbs/ vesicles or sub vesicle formation from the plasma membrane. The streaming speed also depends on the incubation medium in which the specimen was incubated, indicating that the external stimuli as well as internal stimuli can alter the speed of streaming

Download Full-text

Citric Acid-Mediated Abiotic Stress Tolerance in Plants

International Journal of Molecular Sciences ◽

10.3390/ijms22137235 ◽

2021 ◽

Vol 22 (13) ◽

pp. 7235

Author(s):

Md. Tahjib-Ul-Arif ◽

Mst. Ishrat Zahan ◽

Md. Masudul Karim ◽

Shahin Imran ◽

Charles T. Hunter ◽

...

Keyword(s):

Abiotic Stress ◽

Citric Acid ◽

Stress Tolerance ◽

Metabolic Networks ◽

Abiotic Stress Tolerance ◽

Heavy Metal Stress ◽

Stress Conditions ◽

Growth And Yield ◽

Antioxidant Defense Systems ◽

Physiological Outcomes

Several recent studies have shown that citric acid/citrate (CA) can confer abiotic stress tolerance to plants. Exogenous CA application leads to improved growth and yield in crop plants under various abiotic stress conditions. Improved physiological outcomes are associated with higher photosynthetic rates, reduced reactive oxygen species, and better osmoregulation. Application of CA also induces antioxidant defense systems, promotes increased chlorophyll content, and affects secondary metabolism to limit plant growth restrictions under stress. In particular, CA has a major impact on relieving heavy metal stress by promoting precipitation, chelation, and sequestration of metal ions. This review summarizes the mechanisms that mediate CA-regulated changes in plants, primarily CA’s involvement in the control of physiological and molecular processes in plants under abiotic stress conditions. We also review genetic engineering strategies for CA-mediated abiotic stress tolerance. Finally, we propose a model to explain how CA’s position in complex metabolic networks involving the biosynthesis of phytohormones, amino acids, signaling molecules, and other secondary metabolites could explain some of its abiotic stress-ameliorating properties. This review summarizes our current understanding of CA-mediated abiotic stress tolerance and highlights areas where additional research is needed.

Download Full-text

Expression and Co-expression Analyses of WRKY, MYB, bHLH and bZIP Transcription Factor Genes in Potato (Solanum tuberosum) Under Abiotic Stress Conditions: RNA-seq Data Analysis

Potato Research ◽

10.1007/s11540-021-09502-3 ◽

2021 ◽

Author(s):

Ertugrul Filiz ◽

Firat Kurt

Keyword(s):

Transcription Factor ◽

Abiotic Stress ◽

Solanum Tuberosum ◽

Data Analysis ◽

Stress Conditions ◽

Bzip Transcription Factor ◽

Rna Seq ◽

Transcription Factor Genes

Download Full-text

Genome-wide identification and expression analysis of the GA2ox gene family in maize (Zea mays L.) under various abiotic stress conditions

Plant Physiology and Biochemistry ◽

10.1016/j.plaphy.2021.06.043 ◽

2021 ◽

Author(s):

Yidan Li ◽

Xiaohui Shan ◽

Zhilei Jiang ◽

Lei Zhao ◽

Fengxue Jin

Keyword(s):

Zea Mays ◽

Abiotic Stress ◽

Gene Family ◽

Expression Analysis ◽

Zea Mays L ◽

Stress Conditions ◽

Genome Wide

Download Full-text

Citrus plants exude proline and phytohormones under abiotic stress conditions

Plant Cell Reports ◽

10.1007/s00299-017-2214-0 ◽

2017 ◽

Vol 36 (12) ◽

pp. 1971-1984 ◽

Cited By ~ 20

Author(s):

Vicente Vives-Peris ◽

Aurelio Gómez-Cadenas ◽

Rosa María Pérez-Clemente

Keyword(s):

Abiotic Stress ◽

Stress Conditions

Download Full-text

MES7 Modulates Seed Germination via Regulating Salicylic Acid Content in Arabidopsis

Plants ◽

10.3390/plants10050903 ◽

2021 ◽

Vol 10 (5) ◽

pp. 903

Author(s):

Wenrui Gao ◽

Yan Liu ◽

Juan Huang ◽

Yaqiu Chen ◽

Chen Chen ◽

...

Keyword(s):

Salicylic Acid ◽

Salt Stress ◽

Abiotic Stress ◽

Seed Germination ◽

Environmental Conditions ◽

Acid Content ◽

Hydrolytic Enzyme ◽

Stress Conditions ◽

Transitional Period ◽

Highly Sensitive

Seed germination is an important phase transitional period of angiosperm plants during which seeds are highly sensitive to different environmental conditions. Although seed germination is under the regulation of salicylic acid (SA) and other hormones, the molecular mechanism underlying these regulations remains mysterious. In this study, we determined the expression of SA methyl esterase (MES) family genes during seed germination. We found that MES7 expression decreases significantly in imbibed seeds, and the dysfunction of MES7 decreases SA content. Furthermore, MES7 reduces and promotes seed germination under normal and salt stress conditions, respectively. The application of SA restores the seed germination deficiencies of mes7 mutants under different conditions. Taking together, our observations uncover a MeSA hydrolytic enzyme, MES7, regulates seed germination via altering SA titer under normal and abiotic stress conditions.

Download Full-text

The Mechanism of the Tyrosine Transporter TyrP Supports a Proton Motive Tyrosine Decarboxylation Pathway in Lactobacillus brevis

Journal of Bacteriology ◽

10.1128/jb.188.6.2198-2206.2006 ◽

2006 ◽

Vol 188 (6) ◽

pp. 2198-2206 ◽

Cited By ~ 51

Author(s):

Wout A. M. Wolken ◽

Patrick M. Lucas ◽

Aline Lonvaud-Funel ◽

Juke S. Lolkema

Keyword(s):

Phenyl Ring ◽

Lactobacillus Brevis ◽

Proton Motive Force ◽

Proton Pumping ◽

Hydroxyl Group ◽

Transporter Gene ◽

Tyrosine Decarboxylase ◽

Rate Analysis ◽

Substrate Analogues ◽

Decarboxylation Reaction

ABSTRACT The tyrosine decarboxylase operon of Lactobacillus brevis IOEB9809 contains, adjacent to the tyrosine decarboxylase gene, a gene for TyrP, a putative tyrosine transporter. The two genes potentially form a proton motive tyrosine decarboxylation pathway. The putative tyrosine transporter gene of L. brevis was expressed in Lactococcus lactis and functionally characterized using right-side-out membranes. The transporter very efficiently catalyzes homologous tyrosine-tyrosine exchange and heterologous exchange between tyrosine and its decarboxylation product tyramine. Tyrosine-tyramine exchange was shown to be electrogenic. In addition to the exchange mode, the transporter catalyzes tyrosine uniport but at a much lower rate. Analysis of the substrate specificity of the transporter by use of a set of 19 different tyrosine substrate analogues showed that the main interactions between the protein and the substrates involve the amino group and the phenyl ring with the para hydroxyl group. The carboxylate group that is removed in the decarboxylation reaction does not seem to contribute to the affinity of the protein for the substrates significantly. The properties of the TyrP protein are those typical for precursor-product exchangers that operate in proton motive decarboxylation pathways. It is proposed that tyrosine decarboxylation in L. brevis results in proton motive force generation by an indirect proton pumping mechanism.

Download Full-text