A reagent to facilitate protein recovery from cells and tissues

AbstractEconomic growth and the rapid increase in the world population has led to a greater need for natural resources, which in turn, has put pressure on said resources along with the environment. Water, food, and energy, among other resources, pose a huge challenge. Numerous essential resources, including organic substances and valuable nutrients, can be found in wastewater, and these could be recovered with efficient technologies. Protein recovery from waste streams can provide an alternative resource that could be utilized as animal feed. Membrane separation, adsorption, and microbe-assisted protein recovery have been proposed as technologies that could be used for the aforementioned protein recovery. This present study focuses on the applicability of different technologies for protein recovery from different wastewaters. Membrane technology has been proven to be efficient for the effective concentration of proteins from waste sources. The main emphasis of the present short communication is to explore the possible strategies that could be utilized to recover or restore proteins from different wastewater sources. The presented study emphasizes the applicability of the recovery of proteins from various waste sources using membranes and the combination of the membrane process. Future research should focus on novel technologies that can help in the efficient extraction of these high-value compounds from wastes. Lastly, this short communication will evaluate the possibility of integrating membrane technology. This study will discuss the important proteins present in different industrial waste streams, such as those of potatoes, poultry, dairy, seafood and alfalfa, and the possible state of the art technologies for the recovery of these valuable proteins from the wastewater. Graphical abstract

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Protein Recovery after Concentration of Bronchoalveolar Lavage Fluid by Ultrafiltrations

Clinical Science ◽

10.1042/cs068026pa ◽

1985 ◽

Vol 68 (s11) ◽

pp. 26P-26P

Author(s):

S.C. Afford ◽

D. Burnett ◽

J. Kramps ◽

R.A. Stockley

Keyword(s):

Bronchoalveolar Lavage ◽

Bronchoalveolar Lavage Fluid ◽

Lavage Fluid ◽

Protein Recovery

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Generic application of an aqueous two-phase process for protein recovery from animal blood

Process Biochemistry ◽

10.1016/s0032-9592(99)00119-3 ◽

2000 ◽

Vol 35 (7) ◽

pp. 665-673 ◽

Cited By ~ 27

Author(s):

Marco Rito-Palomares ◽

Christopher Dale ◽

Andrew Lyddiatt

Keyword(s):

Protein Recovery ◽

Two Phase ◽

Animal Blood ◽

Phase Process

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Effects of Enzyme Types and Extraction Conditions on Protein Recovery and Antioxidant Properties of Hydrolysed Proteins Derived from Defatted Lemna minor

Applied Science and Engineering Progress ◽

10.14416/j.asep.2021.05.003 ◽

2021 ◽

Author(s):

Hai Chi Tran ◽

Hong Anh Thi Le ◽

Thanh Thanh Le ◽

Van Man Phan

Keyword(s):

Enzymatic Hydrolysis ◽

Antioxidant Activities ◽

Lemna Minor ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Good Method ◽

Alkaline Treatment ◽

Protein Recovery ◽

Hydrolysis Time ◽

Hydrolysis Degree

Lemna minor (L. minor), the common duckweed, contains a high protein substance and is considered as a good source of potential bioactive peptides. The objective of this study is to investigate the effect of enzymatic hydrolysis times (60–180 min) and enzyme concentrations (0.5–3.5%v/w) with Alcalase and Flavourzyme on the recovery, hydrolysis degree (DH), and antioxidant properties of peptides derived from defatted L. minor. The protein recovery, hydrolysis degree (DH), and antioxidant activities obtained by enzymatic were compared with the alkaline treatment method. The results showed that the protein recovery, DH values, and antioxidant activities were enhanced by increasing the enzyme concentration and hydrolysis time. Specifically, the recovery of protein and DH values reached the highest level after the enzymatic hydrolysis by Flavourzyme or Alcalase at 1.5 v/w enzyme for 120 min. At the same enzymatic hydrolysis condition, the samples hydrolyzed by Flavourzyme had a higher inhibitory effect on the ABTS•+ and DPPH•+ radical scavenging than those hydrolyzed by Alcalase and the alkaline treatment. Further study also showed that the DH values, amino acid contents, and antioxidant activities of the protein extracts were positively correlated. Thus, the extractions with Flavourzyme and Alcalse were a good method to produce a significant amount of amino acids and smaller peptides.

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Large Scale Protein Recovery using Aqueous Phase Systems

Separations Using Aqueous Phase Systems ◽

10.1007/978-1-4684-5667-7_48 ◽

1989 ◽

pp. 295-297 ◽

Cited By ~ 1

Author(s):

Maria-Regina Kula

Keyword(s):

Aqueous Phase ◽

Large Scale ◽

Protein Recovery ◽

Phase Systems

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Optimisation of Protein Recovery from Arthrospira platensis by Ultrasound-Assisted Isoelectric Solubilisation/Precipitation

Processes ◽

10.3390/pr8121586 ◽

2020 ◽

Vol 8 (12) ◽

pp. 1586

Author(s):

Ana Sánchez-Zurano ◽

Ainoa Morillas-España ◽

Cynthia Victoria González-López ◽

Tomás Lafarga

Keyword(s):

Food Industry ◽

Functional Foods ◽

Osmotic Shock ◽

Arthrospira Platensis ◽

Extraction Time ◽

Protein Recovery ◽

Solvent Ratio ◽

Total Proteins ◽

Cell Wall Disruption ◽

Pre Treatment

A response surface methodology was used to optimise the solubilisation and precipitation of proteins from the cyanobacterium Arthrospira platensis. Two separate experiments were designed and conducted in a sequential manner. Protein solubilisation was affected by pH, extraction time, and biomass to solvent ratio (p < 0.001). Although spray-drying and the osmotic shock suffered when resuspending the dried biomass into distilled water led to a certain degree of cell wall disruption, the amount of protein that could be solubilised without an additional disruption step was in the range 30–60%. Sequential extractions improved protein solubilisation by less than 5%. For this reason, a pre-treatment based on sonication (400 W, 24 kHz, 2 min) had to be used, allowing the solubilisation of 96.2% of total proteins. Protein precipitation was affected by both pH and extraction time (p < 0.001). The optimised precipitation conditions, which were pH 3.89 over 45 min, led to a protein recovery of 75.2%. The protein content of the extract was close to 80%, which could be further increased by using different purification steps. The proteins extracted could be used in the food industry as technofunctional ingredients or as a source of bioactive hydrolysates and peptides for functional foods and nutraceuticals.

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Oxidative Stress Impairs the Heat Stress Response and Delays Unfolded Protein Recovery

PLoS ONE ◽

10.1371/journal.pone.0007719 ◽

2009 ◽

Vol 4 (11) ◽

pp. e7719 ◽

Cited By ~ 46

Author(s):

Masaaki Adachi ◽

Yaohua Liu ◽

Kyoko Fujii ◽

Stuart K. Calderwood ◽

Akira Nakai ◽

...

Keyword(s):

Oxidative Stress ◽

Heat Stress ◽

Stress Response ◽

Protein Recovery ◽

Heat Stress Response ◽

Unfolded Protein

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Module performance of anion-exchange porous hollow-fiber membranes for high-speed protein recovery

Journal of Chromatography A ◽

10.1016/s0021-9673(97)00687-0 ◽

1997 ◽

Vol 782 (2) ◽

pp. 159-165 ◽

Cited By ~ 24

Author(s):

Noboru Kubota ◽

Yoshitaka Konno ◽

Kyoichi Saito ◽

Kazuyuki Sugita ◽

Kohei Watanabe ◽

...

Keyword(s):

Anion Exchange ◽

Hollow Fiber ◽

High Speed ◽

Protein Recovery ◽

Hollow Fiber Membranes ◽

Fiber Membranes ◽

Module Performance

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High throughput method development and optimised production of leaf protein concentrates with potential to support the agri-industry

Journal of Food Measurement & Characterization ◽

10.1007/s11694-021-01136-w ◽

2021 ◽

Author(s):

Ajay Iyer ◽

Lisa Guerrier ◽

Salomé Leveque ◽

Charles S. Bestwick ◽

Sylvia H. Duncan ◽

...

Keyword(s):

Amino Acid ◽

High Throughput ◽

High Throughput Screening ◽

Protein Extraction ◽

Leaf Protein ◽

Protein Recovery ◽

Screening Methods ◽

Extraction Technology ◽

Protein Concentrates ◽

Ethanol Precipitation

AbstractInvasive plants offer an interesting and unconventional source of protein and the considerable investment made towards their eradication can potentially be salvaged through their revalorisation. To identify viable sources, effective and high-throughput screening methods are required, as well as efficient procedures to isolate these components. Rigorous assessment of low-cost, high-throughput screening assays for total sugar, phenolics and protein was performed, and ninhydrin, Lever and Fast Blue assays were found to be most suitable owing to high reliability scores and false positive errors less than 1%. These assays were used to characterise invasive Scottish plants such as Gorse (Ulex europeans), Broom (Cystisus scoparius) and Fireweed (Chamaenerion angustifolium). Protein extraction (alkali-, heat- and enzyme assisted) were tested on these plants, and further purification (acid and ethanol precipitation, as well as ultrafiltration) procedures were tested on Gorse, based on protein recovery values. Cellulase treatment and ethanol precipitation gave the highest protein recovery (64.0 ± 0.5%) and purity (96.8 ± 0.1%) with Gorse. The amino acid profile of the purified protein revealed high levels of essential amino acids (34.8 ± 0.0%). Comparison of results with preceding literature revealed a strong association between amino acid profiles and overall protein recovery with the extraction method employed. The final purity of the protein concentrates was closely associated to the protein content of the initial plant mass. Leaf protein extraction technology can effectively raise crop harvest indices, revalorise underutilised plants and waste streams.

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