Adequacy of the ELISA Test for Screening Corneal Transplant Donors

SummaryOur previous prospective study of post-infarction patients described a strong and significant association of increased plasma D-dimer concentrations in those who experienced a subsequent coronary death or non-fatal myocardial infarction. In the present study, we compare results on stored plasma obtained two months after the index myocardial infarction from 1,038 patients of this trial, using a simple automated latex agglutination (LA) assay in parallel with the standard ELISA test. Results show a somewhat higher mean value for the LA assay (702 ± 1092 vs. 638 ± 986 ng/ml, p = 0.0002), a strong linear correlation of the two assays (r = 0.86) and 88% agreement for values below 500 ng/ml by the ELISA test. D-dimer concentrations determined by each assay were highly correlated in patients with subsequent coronary artery events (p = 0.93) and quartile values for both the LA and ELISA were equally predictive of such events (p = 0.003 and p = 0.001, respectively). This is the first demonstration that a latex agglutination assay for D-dimer can be used to assess the prognostic risk of recurrent coronary thrombotic disease after myocardial infarction

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Immunodetection of Ostertagia ostertagi in Cattle Milk Samples in Wasit Province

Journal of Education College Wasit University ◽

10.31185/eduj.vol1.iss28.29 ◽

2017 ◽

Vol 1 (28) ◽

pp. 637-648

Author(s):

Abbas Hassan Khlaty Al-Sray

Keyword(s):

Milk Production ◽

Herd Size ◽

Elisa Test ◽

Production Factor ◽

Size Factor ◽

Ostertagia Ostertagi ◽

Milk Samples ◽

Management Group ◽

Breed Group ◽

Age Factor

The aim of present study was to detect the seroprevalence of Ostertagia ostertagi specific antibodies in cattle milk samples in Wasit province, by using the indirect ELISA test for first time in Iraq. For this purpose, an overall 368 dairies cow was submitted for study and the results were revealed that 51 (13.86 %) of tested cows were positive, and the mean optic density ratios (ODRs) of ELISA test values in seropositive cattle were 0.58. Also, this study aimed to investigate an association of seropositive results with some epidemiological risk factors. Hence, the positive results, according to these factors, were as follow: in milk production factor, 6.32 % for ³18 liters/day group, 14.29 % for ³10-18 liters/day group, and 25 % for < 10 liters/day group; in age factor, 13.41% for ³3-6 years group, and 14.75% for >6 years group; in breed factor, 18.27% for local breed group, 12.17% for cross-breed group, and 12% for pure breed group; in farm management factor, 21.35% for bad management group, and 5.68% for good management group; and in herd size factor, 11.59% for <25 (cow/herd) group, and 17.78% for ³ 25 (cow/herd) group. Statistically, the significant differences (P£ 0.05) were observed among related groups of milk production, breed, husbandry management, and herd size factors; while it’s not reported among groups of age factor.

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A case of successful using a stromal corneal transplant for therapeutic keratoplasty in a patient with herpetic keratitis

Oftalmologicheskii Zhurnal ◽

10.31288/oftalmolzh/2018/2/5659 ◽

2018 ◽

Vol 73 (2) ◽

pp. 56-59

Author(s):

E. Sereda ◽

◽

G. Drozhzhyna ◽

T. Gaidamaka ◽

◽

...

Keyword(s):

Herpetic Keratitis ◽

Corneal Transplant

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MONITORING STUDIES OF ARBOVIRUS INFECTIONS TRANSMITTED BY MOSQUITOES ON THE TERRITORY OF THE VOLGOGRAD REGION

ЗДОРОВЬЕ НАСЕЛЕНИЯ И СРЕДА ОБИТАНИЯ - ЗНиСО / PUBLIC HEALTH AND LIFE ENVIRONMENT ◽

10.35627/2219-5238/2019-315-6-60-66 ◽

2019 ◽

pp. 60-66

Author(s):

E.V. Molchanova ◽

D.N. Luchinin ◽

A.O. Negodenko ◽

D.R. Prilepskaya ◽

N.V. Boroday ◽

...

Keyword(s):

Virus Antigen ◽

Elisa Test ◽

Serum Samples ◽

Blood Sucking ◽

Tick Borne Encephalitis ◽

Volgograd Region ◽

Two Samples ◽

Probable Presence ◽

California Serogroup ◽

Tick Borne Encephalitis Virus

The paper presents data from the monitoring studies’ results of arbovirus infections transmitted by mosquitoes in the Volgograd region. West Nile virus antigen (WNV) in 9 samples, Tahyna virus in one sample, Batai virus in two samples were detected in the study of 110 samples of field material (blood-sucking mosquitoes) by ELISA test. Antibodies to WNV in 16.58 percent of the samples, to tick-borne encephalitis virus in 1.08 percent, to viruses of the California serogroup and Ukuniemi in 1.09 percent, to the virus Sindbis in 2.17 percent were detected as a result of the study of blood serum samples from donors in the Volgograd region. Thus, we obtained data on the probable presence of the Batai, Sindbis, Ukuniemi and Californian serogroup viruses along with the circulation of WNV on the territory of the Volgograd region.

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Statistical Quality Control of HIV-1 ELISA Test Performance

Journal of the American Statistical Association ◽

10.1080/01621459.1994.10476861 ◽

1994 ◽

Vol 89 (428) ◽

pp. 1200-1208 ◽

Cited By ~ 3

Author(s):

R. C. Gentleman ◽

M. S. Hamada ◽

D. E. Matthews ◽

A. R. Wilson

Keyword(s):

Quality Control ◽

Test Performance ◽

Elisa Test ◽

Statistical Quality Control ◽

Statistical Quality ◽

Hiv 1

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Molecular characteristics of HBV infection among blood donors tested HBsAg reactive in a single ELISA test in southern China

BMC Infectious Diseases ◽

10.1186/s12879-020-05747-4 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Xianlin Ye ◽

Tong Li ◽

Ran Li ◽

Heng Liu ◽

Junpeng Zhao ◽

...

Keyword(s):

Viral Load ◽

Hepatitis B ◽

Nested Pcr ◽

Blood Donors ◽

Southern China ◽

Core Promoter ◽

Elisa Test ◽

Hbv Infection ◽

Molecular Characteristics ◽

Blood Samples

Abstract Background Hepatitis B virus (HBV) infection is a major concern for blood safety in high-prevalence HBV countries such as China. In Shenzhen, dual hepatitis B surface antigen (HBsAg) enzyme-linked immunosorbent assays (ELISAs) have been adopted in parallel with nucleic acid testing (NAT) for donors for over a decade. A small proportion of blood donors test reactive (R) for HBsAg but negative through routine NAT, which can lead to HBV infection with an extremely low viral load. Objectives We aimed to investigate and analyze the molecular characteristics of HBV among blood donors that tested HBsAg R in a single ELISA test. Methods Blood donations were evaluated in this study if confirmed HBsAg R through one of two ELISA kits. Samples with non-reactive (NR) results by NAT were collected and tested for HBsAg by chemiluminescent microparticle immunoassay (CLIA) with a neutralization test. The level of HBsAg was further assessed by electrochemiluminescence immunoassay (ECLIA). The viral basic core promoter (BCP) and pre-core (PC) and S regions were amplified by nested PCR. Quantitative real-time PCR (qPCR) for viral load determination and individual donation (ID)-NAT were adopted simultaneously. HBsAg was confirmed with CLIA, ECLIA, nested PCR, qPCR, and ID-NAT. Results Of the 100,252 donations, 38 and 41 were identified as HBsAg R with Wantai and DiaSorin ELISA kits, respectively. Seventy-nine (0.077%, 79/100,252) blood samples with ELISA R-NR and NAT NR results were enrolled in the study. Of these, 17 (21.5%,17/79) were confirmed as HBsAg-positive. Of the 14 genotyped cases, 78.6% (11/14) were genotype B, and C and D were observed in two and one sample, respectively. Mutations were found in the S gene, including Y100C, Y103I, G145R, and L175S, which can affect the detection of HBsAg. A high-frequency mutation, T1719G (93.3%), was detected in the BCP/PC region, which reduced the viral replication. Conclusion A small number of blood samples with HBsAg ELISA R-NR and NAT NR results were confirmed as HBV infection, viral nucleic acids were found in most of the samples through routine NAT methods. It is necessary to employ more sensitive and specific assays for the detection of HBV infection among blood donors.

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First report of human infection caused by Colletotrichum chlorophyti occurring in a post-corneal transplant patient with endophthalmitis

Medical Mycology Case Reports ◽

10.1016/j.mmcr.2021.04.002 ◽

2021 ◽

Vol 32 ◽

pp. 73-76

Author(s):

Alberto E. Paniz-Mondolfi ◽

Steven Agemy ◽

Connie Cañete-Gibas ◽

Melissa R. Gitman ◽

Codrin E. Iacob ◽

...

Keyword(s):

Transplant Patient ◽

Human Infection ◽

Corneal Transplant ◽

First Report

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Analysis of a Routinely Used Commercial Anti-Chikungunya IgM ELISA Reveals Cross-Reactivities with Dengue in Brazil: A New Challenge for Differential Diagnosis?

Diagnostics ◽

10.3390/diagnostics11050819 ◽

2021 ◽

Vol 11 (5) ◽

pp. 819

Author(s):

Monique da Rocha Queiroz Lima ◽

Raquel Curtinhas de Lima ◽

Elzinandes Leal de Azeredo ◽

Flavia Barreto dos Santos

Keyword(s):

Disease Surveillance ◽

Endemic Area ◽

Chikungunya Virus ◽

Elisa Test ◽

Cross Reactivity ◽

Immunoglobulin M ◽

Diagnostic Tools ◽

Healthy Individuals ◽

Denv Serotypes ◽

Igm Elisa

In Brazil, chikungunya emerged in 2014, and by 2016, co-circulated with other arbovirosis, such as dengue and zika. ELISAs (Enzyme-Linked Immunosorbent Assays) are the most widely used approach for arboviruses diagnosis. However, some limitations include antibody cross reactivities when viruses belong to the same genus, and sensitivity variations in distinct epidemiological scenarios. As chikungunya virus (CHIKV) is an alphavirus, no serological cross reactivity with dengue virus (DENV) should be observed. Here, we evaluated a routinely used chikungunya commercial IgM (Immunoglobulin M) ELISA test (Anti-Chikungunya IgM ELISA, Euroimmun) to assess its performance in confirming chikungunya in a dengue endemic area. Samples (n = 340) representative of all four DENV serotypes, healthy individuals and controls were tested. The Anti-CHIKV IgM ELISA test had a sensitivity of 100% and a specificity of 25.3% due to the cross reactivities observed with dengue. In dengue acute cases, the chikungunya test showed an overall cross-reactivity of 31.6%, with a higher cross-reactivity with DENV-4. In dengue IgM positive cases, the assay showed a cross-reactivity of 46.7%. Serological diagnosis may be challenging and, despite the results observed here, more evaluations shall be performed. Because distinct arboviruses co-circulate in Brazil, reliable diagnostic tools are essential for disease surveillance and patient management.

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Validation of a Novel Commercial ELISA Test for the Detection of Antibodies against Coxiella burnetii

Pathogens ◽

10.3390/pathogens9121075 ◽

2020 ◽

Vol 9 (12) ◽

pp. 1075

Author(s):

Salvatore Ledda ◽

Cinzia Santucciu ◽

Valentina Chisu ◽

Giovanna Masala

Keyword(s):

Diagnostic Accuracy ◽

Phase Ii ◽

Coxiella Burnetii ◽

Q Fever ◽

Animal Health ◽

Elisa Test ◽

Clinical Diagnostics ◽

Complex Life Cycle ◽

Enzyme Linked Immunosorbent Assay ◽

Specificity And Sensitivity

Q fever is a zoonosis caused by Coxiella burnetii, a Gram-negative pathogen with a complex life cycle and a high impact on public and animal health all over the world. The symptoms are indistinguishable from those belonging to other diseases, and the disease could be symptomless. For these reasons, reliable laboratory tests are essential for an accurate diagnosis. The aim of this study was to validate a novel enzyme-linked immunosorbent assay (ELISA) test, named the Chorus Q Fever Phase II IgG and IgM Kit (DIESSE, Diagnostica Senese S.p.A), which is performed by an instrument named Chorus, a new device in medical diagnostics. This diagnostic test is employed for the detection of antibodies against C. burnetii Phase II antigens in acute disease. Our validation protocol was performed according to the Italian Accreditation Body (ACCREDIA) (Regulation UNI CEI EN ISO/IEC 17025:2018 and 17043:2010), OIE (World Organization for Animal Health), and Statement for Reporting Studies of Diagnostic Accuracy (STARD). Operator performance was evaluated along with the analytical specificity and sensitivity (ASp and ASe) and diagnostic accuracy of the kit, with parameters such as diagnostic specificity and sensitivity (DSp and DSe) and positive and negative predictive values (PPV and NPV), in addition to the repeatability. According to the evaluated parameters, the diagnostic ELISA test was shown to be suitable for validation and commercialization as a screening method in human sera and a valid support for clinical diagnostics.

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