Algae-derived β-glucan enhanced gut health and immune responses of weaned pigs experimentally infected with a pathogenic E. coli

2019 ◽  
Vol 248 ◽  
pp. 114-125 ◽  
Author(s):  
Kwangwook Kim ◽  
Amy Ehrlich ◽  
Vivian Perng ◽  
Jennifer A. Chase ◽  
Helen Raybould ◽  
...  
Keyword(s):  
Immune Responses ◽  
Gut Health ◽  
Weaned Pigs ◽  
E Coli

scholarly journals Growth performance and gut health of Escherichia coli-challenged weaned pigs fed diets supplemented with a Bacillus subtilis direct-fed microbial

2020 ◽  
Vol 4 (3) ◽  
Author(s):  
Sangwoo Park ◽  
Jung Wook Lee ◽  
Kevin Jerez Bogota ◽  
David Francis ◽  
Jolie Caroline González-Vega ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Bacillus Subtilis ◽  
Growth Performance ◽  
Feed Efficiency ◽  
Basal Diet ◽  
Feed Additives ◽  
Growth Promoter ◽  
Gut Health ◽  
Weaned Pigs ◽  
E Coli

Abstract This study was conducted to investigate the effects of a direct-fed microbial (DFM) product (Bacillus subtilis strain DSM 32540) in weaned pigs challenged with K88 strain of Escherichia coli on growth performance and indicators of gut health. A total of 21 weaned pigs [initial body weight (BW) = 8.19 kg] were housed individually in pens and fed three diets (seven replicates per diet) for 21 d in a completely randomized design. The three diets were a corn-soybean meal-based basal diet without feed additives, a basal diet with 0.25% antibiotics (neo-Oxy 10-10; neomycin + oxytetracycline), or a basal diet with 0.05% DFM. All pigs were orally challenged with a subclinical dose (6.7 × 108 CFU/mL) of K88 strain of E. coli on day 3 of the study (3 d after weaning). Feed intake and BW data were collected on days 0, 3, 7, 14, and 21. Fecal scores were recorded daily. On day 21, pigs were sacrificed to determine various indicators of gut health. Supplementation of the basal diet with antibiotics or DFM did not affect the overall (days 0–21) growth performance of pigs. However, antibiotics or DFM supplementation increased (P = 0.010) gain:feed (G:F) of pigs during the post-E. coli challenge period (days 3–21) by 23% and 24%, respectively. The G:F for the DFM-supplemented diet did not differ from that for the antibiotics-supplemented diet. The frequency of diarrhea for pigs fed a diet with antibiotics or DFM tended to be lower (P = 0.071) than that of pigs fed the basal diet. The jejunal villous height (VH) and the VH to crypt depth ratio (VH:CD) were increased (P < 0.001) by 33% and 35%, respectively, due to the inclusion of antibiotics in the basal diet and by 43% and 41%, respectively due to the inclusion of DFM in the basal diet. The VH and VH:CD for the DFM-supplemented diet were greater (P < 0.05) than those for the antibiotics-supplemented diet. Ileal VH was increased (P < 0.05) by 46% due to the inclusion of DFM in the basal diet. The empty weight of small intestine, cecum, or colon relative to live BW was unaffected by dietary antibiotics or DFM supplementation. In conclusion, the addition of DFM to the basal diet improved the feed efficiency of E. coli-challenged weaned pigs to a value similar to that of the antibiotics-supplemented diet and increased jejunal VH and VH:CD ratio to values greater than those for the antibiotics-supplemented diet. Thus, under E. coli challenge, the test DFM product may replace the use of antibiotics as a growth promoter in diets for weaned pigs to improve feed efficiency and gut integrity.

Levamisole stimulates proliferation of circulating and intestinal immune cell subsets, gut health and performance in weaned pigs

2014 ◽  
Vol 94 (1) ◽  
pp. 43-53 ◽  
Author(s):  
Hrvoje Valpotić ◽  
Marcela Šperanda ◽  
Ana Kovšca-Janjatović ◽  
Mislav Ðidara ◽  
Gordana Lacković ◽  
...  
Keyword(s):  
Peripheral Blood ◽  
Immune Cell ◽  
Lymphoid Cells ◽  
Immune Functions ◽  
Gut Health ◽  
Weaned Pigs ◽  
E Coli ◽  
Immune Cell Subsets ◽  
Improved Performance ◽  
And Performance

Valpotić, H., Šperanda, M., Kovšca-Janjatović, A., Ðidara, M., Lacković, G., Božić, F., Habrun, B., Srečec, S., Mataušić-Pišl, M. and Valpotić, I. 2014. Levamisole stimulates proliferation of circulating and intestinal immune cell subsets, gut health and performance in weaned pigs. Can. J. Anim. Sci. 94: 43–53. With the growing knowledge of the porcine immune system and its endogenous modulation, it has been clearly stated that exogenous modulation through the use of substances able to modulate immune functions represents an important prophylactic/therapeutic approach in prevention/treatment of both stress- and F4+ and F18+ enterotoxigenic E. coli (ETEC)-induced infections accompanied weaning. The aim of this study was to evaluate the effectiveness of levamisole (LEVA; 2.5 mg kg−1 BW in 10 mL) applied per os to weaned pigs in proliferation of circulating and intestinal immune cell subsets throughout a period of 5 wk. Changes in proportion or number of peripheral blood and ileal mucosal leukocytes tested were studied either weekly by flow cytometry or at the end of the experiment (day 35) by immunohistology/histomorphometry, respectively. Pigs treated with LEVA had increased proportions of peripheral blood CD45+ lymphoid cells, CD4+ and CD8+ T cells, and CD21+ B cells (P<0.01) between days 14 and 35 following the treatment. Also, LEVA stimulated the proliferation of CD45RA+ naïve lymphoid cells in interfollicular (P<0.001) and follicular areas (P<0.05) of ileal Peyer’s patches at day 35 of the experiment. These pigs had a significantly higher (P<0.05) average body weight (19.7 vs. 17.1 kg) and weight gain at the end of experiment compared with the control pigs (for 15%). We conclude that LEVA stimulated the proliferation of circulating and intestinal lymphoid cell subsets tested and improved performance in weaned pigs, and thus, the drug may nonspecifically enhance their immunity/resistance to F4+ and F18+ ETEC strains.

scholarly journals 175 Effects of dietary supplementation with xylanase and probiotics on growth performance and gut health of newly weaned pigs challenged with enterotoxigenic E. coli on d 7 post weaned

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 78-78
Author(s):  
Marcos E Duarte ◽  
James Tyus ◽  
Sung Woo Kim
Keyword(s):  
Growth Performance ◽  
Dietary Supplementation ◽  
Protein Carbonyl ◽  
Gut Health ◽  
Weaned Pigs ◽  
Ki 67 ◽  
Nursery Pigs ◽  
E Coli ◽  
Crypt Depth ◽  
Fecal Score

Abstract This study was to investigate the effect of dietary supplementation with xylanase and probiotic on growth performance, and gut health of nursery pigs challenged with ETEC. Sixty-four weaned pigs (7.9 ± 0.4 kg) were allotted in a RCBD (2 x 2 factorial). ETEC (0, and ETEC, 6 x 109 CFU/mL) and synbiotic (0, and xylanase 10,000 XU/kg combined with Bacillus sp. 6 x 108 CFU/kg) were the factors. ETEC was orally inoculated on d 7. Growth performance were measured on d 7, 10, 15, and 21. On d 21, 48 pigs were euthanized for sampling to measure gut health parameters. Synbiotic increased (P &lt; 0.05) ADG in P1 (53.5 to 96.0 g). Overall, ETEC reduced (P &lt; 0.05) ADG (387.5 to 322.5 g) and G:F (0.810 to 0.706). ETEC increased (P &lt; 0.05) fecal score from d 7 to 13, whereas synbiotic reduced (P &lt; 0.05) it at d 9 and 11 in challenged pigs. ETEC increased (P &lt; 0.05) MDA (0.259 to 0.818 μmol/mg), IL6 (2.96 to 4.30 pg/mg), ki-67+ (29.1 to 33.9%), and crypt depth (260 to 290 μm), whereas synbiotic tended to reduce TNF-α (1.05 to 0.87 pg/mg), protein carbonyl (3.13 to 2.51 nmol/mg), and IL6 (4.07 to 3.19 pg/mg); reduced (P &lt; 0.05) crypt depth (290 to 260 μm), and ki-67+ (32.7 to 30.3%) and increased (P &lt; 0.05) villus height (368.5 to 421.4 μm). ETEC reduced (P &lt; 0.05) abundance of Veillonellaceae (7.11 to 3.02%), tended to reduce (P = 0.067) Clostridiaceae (1.1 to 0.55%), and Prevotellaceae (38.0 to 27.2%) and tended (P = 0.063) to increase Helicobacteraceae (34.5 to 49.5%). Collectively, ETEC reduced growth performance by affecting the microbiome, oxidative stress, and immune response. Synbiotic improve growth performance by enhancing gut health regardless of the challenge, whereas it reduced fecal score in challenged pigs.

scholarly journals PSVII-42 Growth performance, gut health, and immune responses of Escherichia coli-challenged weaned pigs fed probiotic-supplemented diets

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 359-360
Author(s):  
Sangwoo Park ◽  
Jinsu Hong ◽  
David Francis ◽  
Caroline J González -Vega ◽  
John K Htoo ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Growth Performance ◽  
Immunoglobulin A ◽  
Dietary Treatment ◽  
Basal Diet ◽  
Gut Health ◽  
Weaned Pigs ◽  
E Coli ◽  
Randomized Design ◽  
Villous Height

Abstract To evaluate the effects of probiotics on performance and gut health of Escherichia coli-challenged weaned pigs, a total of 28 weaned pigs (initial BW = 8.25 kg) were housed individually in pens and fed 4 experimental diets for 21 d in a completely randomized design. The 4 diets were corn-soybean meal-based basal diet without or with 0.25% antibiotics (neomycin + oxytetracycline), 0.05% Bacillus subtilis DSM 32540, or 0.05% Bacillus pumilus DSM 32539. All pigs were orally challenged with K88 strain of E. coli on d 3. Feed intake and BW data, and blood samples were collected on d 3, 7, 14, and 21. Fecal scores were recorded daily. On d 21, pigs were sacrificed to determine gastrointestinal histomorphology, pH and organ weights. Supplementation of the basal diet with antibiotics or B. pumilus did not affect the overall (d 0 to 21) ADG and G:F. The B. subtilis supplementation did not affect the overall ADG, but tended to increase (P &lt; 0.1) the overall G:F by 15%. Fecal score was unaffected by dietary treatment. Supplementation of the basal diet with either probiotic, but not antibiotics increased (P &lt; 0.05) jejunal villous height and reduced (P &lt; 0.05) cecal digesta pH. Supplementation with B. pumilus tended to increase (P &lt; 0.1) colon weight relative to BW; however, antibiotics or B. subtilis supplementation did not affect colon weight. The addition of B. subtilis to basal diet tended to increase (P &lt; 0.1) serum immunoglobulin A concentration on d 21 of the study; however, such effect was not observed with the addition of antibiotics or B. pumilus. In conclusion, dietary B. subtilis improved G:F and jejunal villous height and reduced cecal digesta pH, indicating that dietary supplementation with B. subtilis at 0.05% could improve growth performance and gut health of weaned pigs.

scholarly journals Growth performance and gut health of Escherichia coli-challenged weaned pigs fed canola meal-containing diet

2021 ◽  
Author(s):  
Jinsu Hong ◽  
Samuel Ariyibi ◽  
Linto Antony ◽  
Joy Scaria ◽  
Steven Dilberger-Lawson ◽  
...  
Keyword(s):  
Blood Cells ◽  
White Blood Cells ◽  
Basal Diet ◽  
Liver Weight ◽  
Canola Meal ◽  
Gut Health ◽  
Weaned Pigs ◽  
E Coli ◽  
D 20 ◽  
Fecal Score

Abstract An experiment was conducted to evaluate the effects of including canola meal (CM) in diets for weaning pigs challenged with a F18 strain of E. coli on growth performance and gut health. A total of 36 individually housed weaned pigs [initial body weight (BW) = 6.22 kg] were randomly allotted to 1 of the 3 diets (12 pigs/diets). The 3 diets were corn-soybean meal (SBM)-based basal diet (control diet) and the basal diet with 0.3% zinc oxide, 0.2% chlortetracycline, and 0.2% tiamulin (antibiotic diet), or with 20% canola meal (CM diet). The diets were fed in 2 phases; Phase 1: d 0 to 7, and Phase 2: d 7 to 20. All pigs were given an oral dose of 2 × 10 9 CFU of F18 strain of E. coli on d 7. Fecal score was assessed daily throughout the trial. Dietary antibiotics increased (P &lt; 0.05) overall ADG and ADFI compared by 48% and 47%, respectively. Dietary CM increased (P &lt; 0.05) overall ADG and ADFI by 22% and 23%, respectively; but the ADG and ADFI values for CM-containing diet did not reach those for the antibiotics-containing diet. Dietary antibiotics reduced (P &lt; 0.05) fecal score; however, dietary CM unaffected fecal score. Dietary antibiotics decreased (P &lt; 0.05) liver weight per unit live BW by 16% at d 20; whereas dietary CM did not affect liver weight per unit live BW (29.2 vs. 28.6). Also, dietary antibiotics increased (P &lt; 0.05) serum triiodothyronine and tetraiodothyronine levels for d 14; whereas dietary CM did not affect the serum level of these hormones. Dietary antibiotics reduced (P &lt; 0.05) the number white blood cells and neutrophils by 38% and 43% at d 20, respectively; whereas dietary CM tended to reduce (P = 0.09) the number white blood cells by 19% at d 20. The number white blood cells for CM diet tended to be greater (P &lt; 0.10) than that for antibiotics diet. The dietary antibiotics decreased (P &lt; 0.05) the concentration of individual volatile fatty acids and hence of total volatile fatty acid in cecum by 61% at d 20; whereas dietary CM decreased (P &lt; 0.05) cecal butyric acid concentration by 61% and tended to reduce (P &lt; 0.10) total volatile fatty acid concentration by 30% at d 20. In conclusion, the dietary inclusion of 20% canola meal improved ADG and tended to reduce white blood cell counts. Thus, inclusion of CM in antibiotics-free corn-SBM-based diets for weaned pigs that are challenged with F18 strain of E. coli can result in their improved performance partly through a reduction of the inflammatory response.

scholarly journals PSIV-11 Effects of very low-dose antibiotics on gene expression profiles in ileal mucosa of weaned pigs infected with a pathogenic E. coli

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 286-286
Author(s):  
Kwangwook Kim ◽  
Sungbong Jang ◽  
Yanhong Liu
Keyword(s):  
Gene Expression ◽  
Systemic Inflammation ◽  
Low Dose ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Post Inoculation ◽  
Growth Promoter ◽  
Weaned Pigs ◽  
Ileal Mucosa ◽  
E Coli

Abstract Our previous studies have shown that supplementation of low-dose antibiotic growth promoter (AGP) exacerbated growth performance and systemic inflammation of weaned pigs infected with pathogenic Escherichia coli (E. coli). The objective of this experiment, which is extension of our previous report, was to investigate the effect of low-dose AGP on gene expression in ileal mucosa of weaned pigs experimentally infected with F18 E. coli. Thirty-four pigs (6.88 ± 1.03 kg BW) were individually housed in disease containment rooms and randomly allotted to one of three treatments (9 to 13 pigs/treatment). The three dietary treatments were control diet (control), and 2 additional diets supplemented with 0.5 or 50 mg/kg of AGP (carbadox), respectively. The experiment lasted 18 d [7 d before and 11 d after first inoculation (d 0)]. The F18 E. coli inoculum was orally provided to all pigs with the dose of 1010 cfu/3 mL for 3 consecutive days. Total RNA [4 to 6 pigs/treatment on d 5; 5 to 7 pigs/treatment on 11 post-inoculation (PI)] was extracted from ileal mucosa to analyze gene expression profiles by Batch-Tag-Seq. The modulated differential gene expression were defined by 1.5-fold difference and a cutoff of P &lt; 0.05 using limma-voom package. All processed data were statistically analyzed and evaluated by PANTHER classification system to determine the biological process function of genes in these lists. Compared to control, supplementation of recommended-dose AGP down-regulated genes related to inflammatory responses on d 5 and 11 PI; whereas, feeding low-dose AGP up-regulated genes associated with negative regulation of metabolic process on d 5, but down-regulated the genes related to immune responses on d 11 PI. The present observations support adverse effects of low-dose AGP in our previous study, indicated by exacerbated the detrimental effects of E. coli infection on pigs’ growth rate, diarrhea and systemic inflammation.

PSIII-16 Growth Performance of Weaned Pigs Fed Barley Differing in Fermentable Starch and Fiber Profile

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 175-176
Author(s):  
Joaquin J Sanchez Zannatta ◽  
L F Wang ◽  
Eduardo Beltranena ◽  
Aaron D Beattie ◽  
Rex N Newkirk ◽  
...  
Keyword(s):  
Energy Source ◽  
Growth Performance ◽  
Steam Explosion ◽  
Phase 1 ◽  
Average Daily Gain ◽  
Barley Grain ◽  
Gut Health ◽  
Weaned Pigs ◽  
Cereal Grain ◽  
Hulled Barley

Abstract Barley grain containing more fermentable starch or fiber might be an attractive energy source in weaned pig diets due to benefits on gut health. Barley rapidly-fermentable carbohydrates may serve as prebiotic and slowly-fermentable fiber may decrease diarrhea in weaned pigs. Steam-explosion processing may disrupt the fiber matrix of hulls, increasing slowly-fermentable fiber of barley. To explore, 220 pigs were fed 1 of 5 diets containing 60% cereal grain: 1) low-fermentable hulled barley (LFB); 2) LFB steam-exploded (LFB-E; 1.2 MPa, 120 s); 3) high β-glucan (10% DM) hull-less barley (HFB); 4) high amylose (17% DM) hull-less barley (HFA); or 5) low-fermentable wheat (LFW). Diets were fed starting 1-week post-weaning and formulated to provide 2.4 and 2.3 Mcal net energy (NE)/kg, 5.5 and 5.1 g standardized ileal digestible lysine/Mcal NE for phase 1 (day 1–14) and phase 2 (day 15–35), respectively. For the entire trial (day 1–35), average daily feed intake (ADFI) and average daily gain (ADG) of pigs did not differ among diets. Gain:feed (G:F) did not differ between LFB and LFW diets, but steam-explosion of hulled barley reduced (P &lt; 0.05) G:F. Feces consistency did not differ between LFB and LFW diets, but was better (P &lt; 0.05) for LFB than HFB, HFA and LFB-E diets. For phase 1, G:F of pigs was lower (P &lt; 0.05) for LFB-E diet than LFW diet. For days 22–28, LFB-E diet had greater (P &lt; 0.01) ADFI than HFA diet and tended (P = 0.09) to have a greater ADG than HFB diet. In conclusion, hulled or hull-less barley grain replaced wheat grain without affecting growth performance in weaned pigs. Hulled barley increased feces consistency. Steam-explosion of hulled barley did not increase growth performance of weaned pigs. Barley grain is an attractive energy source for weaned pigs for managing growth and feces consistency.

Amplification of immune responses against a DNA-delivered idiotypic lymphoma antigen by fusion to the B subunit of E. coli heat labile toxin

Vaccine ◽  
2009 ◽  
Vol 27 (32) ◽  
pp. 4289-4296 ◽  
Author(s):  
Caleb G. Chen ◽  
Yen-Ta Lu ◽  
Marie Lin ◽  
Natalia Savelyeva ◽  
Freda K. Stevenson ◽  
...  
Keyword(s):  
Immune Responses ◽  
E Coli ◽  
B Subunit ◽  
Heat Labile
Sign in / Sign up

Export Citation Format

Share Document