Antioxidant status, chemical composition and fermentation profile of alfalfa silage ensiled at two dry matter contents with a novel Lactobacillus plantarum strain with high-antioxidant activity

Author(s):  
Y.X. Zhang ◽  
Z.P. Huang ◽  
D. Vyas ◽  
A.T. Adesogan ◽  
M. Franco ◽  
...  
Molecules ◽  
2021 ◽  
Vol 26 (12) ◽  
pp. 3741
Author(s):  
Wioleta Pietrzak ◽  
Renata Nowak

The content of plant secondary metabolites is not stable, and factors such as the region/location effect and seasonal variations have an impact on their chemical composition, especially in parasitic plants. Research in this area is an important step in the development of quality parameter standards of medicinal plants and their finished products. The effects of the time and place of harvest and the host tree species on the chemical composition and antioxidant activity of mistletoe extracts were investigated. Statistical tools were used to evaluate the results of the spectrophotometric and LC-ESI-MS/MS studies of the phenolic composition and antioxidant activity. The investigations indicate that the qualitative and quantitative composition, influencing the biological activity of mistletoe extracts, largely depends on the origin of the plant. The mistletoe extracts exhibited a rich phenol profile and high antioxidant activity. The chemometric analysis indicated that mistletoe collected from conifers (Viscum abietis and Viscum austriacum) had the most advantageous chemical composition and antioxidant activity. Moreover, the chemical profile and biological activity of the plant material were closely related to the climatic conditions and location of the harvested plant. Higher levels of phenolic compounds and high antioxidant activity were found in extracts obtained from plant material collected in cold weather with the presence of snow and less sunshine (autumn–winter period).


2019 ◽  
Vol 11 (4) ◽  
pp. 197
Author(s):  
Diogo Mendes da Silva ◽  
Suzan Kelly Vilela Bertolucci ◽  
Smail Aazza ◽  
Alexandre Alves de Carvalho ◽  
Simony Carvalho Mendonça ◽  
...  

The purpose of the present work was to evaluate the vegetative growth of Mentha piperita L. cultivated under different water availability, as well its influence in content, chemical composition and in vitro antioxidant activity of its essential oil. Plants were propagated by mother plants microcutting and scions were transplanted to 5 L pots with soil and cattle manure. Afterward, were kept at field capacity for 30 days and under treatment for 40 days. It was treated with different levels of water deficit treatments: (T1): 100 of field capacity (FC); (T2): 80 of FC; (T3): 60 of FC; (T4) 40 of FC with 5 blocks. Vegetative growth was evaluated by dry matter contents of all part of plants and by root/aerial rate. The essential oil of the leaves was extracted by hydrodistillation, analyzed by GC-FID and GC-MS and in vitro antioxidant potential was evaluated. A significant decrease in the dry matter of leaves and stems accompanied with a decrease in the roots dry matter was observed with an increase in the water stress. Quantitative chemical differences were observed in the chemical composition of the essential oil, according water availability. Total antioxidant activity showed a gradual increase as water stress progressed.


2021 ◽  
Vol 51 (3) ◽  
pp. 191-198
Author(s):  
Juliana Schuch PITIRINI ◽  
Rosana Ingrid Ribeiro dos SANTOS ◽  
Francy Manoely da Silva LIMA ◽  
Ilano Silva Braga do NASCIMENTO ◽  
Jehmison de Oliveira BARRADAS ◽  
...  

ABSTRACT The use of cassava root silage for animal feeding is a suitable option for farmers who grow cassava as an alternative product and for cattle ranchers who have to deal with high prices of corn. Our objective was to determine the effects of cassava genotypes and the correction of soil acidity on the microbial population, fermentation characteristics, chemical composition, aerobic stability and losses of cassava root silage. We used a 2 × 3 factorial design in completely randomized blocks, with four replications. We evaluated two cassava genotypes (Caeté and Manteiguinha) and three methods of soil acidity correction (lime, gypsum, and lime+gypsum). The roots were harvested 11 months after planting, ensiled in PVC silos, and stored for 45 days. No interaction was observed between genotypes and soil acidity correction for any of the evaluated parameters. The silage of Caeté genotype showed the highest concentration of dry matter (421 g kg-1 fresh matter) and non-fibrous carbohydrates (893 g kg-1 dry matter), and the lowest concentrations of neutral detergent fiber (37.1 g kg-1 dry matter) . No significant differences were observed among treatments for lactic acid bacteria, yeast and mold counts in silages. Both genotypes resulted in silages with an adequate fermentation profile and considerably high aerobic stability, but with high effluent loss. The Caeté genotype showed to be potentially better for silage production due to its higher dry matter recovery. Due to the high level of effluent loss, it is recommended to test the effect of a moisture-absorbing additive during the ensiling process of these cassava roots.


2019 ◽  
Vol 59 (4) ◽  
pp. 721
Author(s):  
Lucas Ladeira Cardoso ◽  
Karina Guimarães Ribeiro ◽  
Marcos Inácio Marcondes ◽  
Odilon Gomes Pereira ◽  
Kirsten Weiß

Sugarcane silage can be used in animal production systems; however, it is important to apply additives to improve its chemical composition and fermentative quality. We evaluated the effect of chemical (urea and calcium oxide (CaO)) and microbial (Lactobacillus buchneri (LB), Lactobacillus plantarum, Pediococcus pentosaceus, and Propionibacterium acidipropionici) additives on chemical composition, fermentation profile, microorganism population, and production of ethanol and other volatile organic compounds in sugarcane silage. Treatments studied were silage without inoculant (SS), SS with LB, SS with Lactobacillus plantarum and Pediococcus pentosaceus, SS with Lactobacillus plantarum and Propionibacterium acidipropionici, SS with 5 g CaO/kg fresh material (FM) (5CaO), SS with 10 g CaO/kg FM (10CaO), SS with 5 g urea/kg FM (5urea), and SS with 10 g urea/kg FM (10urea). The highest crude protein content (P = 0.001) and the lowest N-linked to fibre content (P = 0.001) occurred when applying urea. None of the treatments reduced the presence of yeast (P = 0.054), but a trend was detected of treatments based on CaO as promising in this Control. The silages treated with CaO had lower ethyl ester and ethanol (average for CaO-based treatments of 0.012 g/kg dry matter and 0.695 g/kg dry matter, respectively), and silages treated with 10urea had less acetone (P = 0.001) and methanol (P = 0.001). The sugarcane silages treated with chemical additive CaO reduced ethanol production and ester formation. There was a high correlation (r = 0.984) between ethyl acetate + ethyl lactate and ethanol contents.


Molecules ◽  
2018 ◽  
Vol 24 (1) ◽  
pp. 51 ◽  
Author(s):  
Zhongxi Li ◽  
Jing Teng ◽  
Yilu Lyu ◽  
Xiaoqian Hu ◽  
Yueliang Zhao ◽  
...  

The present study examined the effect of Lactobacillus plantarum ATCC14917 fermentation on the chemical composition and antioxidant activity of apple juice. Apple juice was fermented and examined of its antioxidant activity using chemical models and cellular antioxidant assay. Furthermore, the chemical composition of fermented apple juice was characterized by LC-MS/MS. Lactobacillus plantarum ATCC14917 fermentation showed an increase in DPPH and ABTS radical scavenging activity as well as cellular antioxidant activity of apple juice. However, fermentation decreased the total phenolic and flavonoid content. Subsequent LC-MS/MS analysis of the phenolic profile indicated that the content of 5-O-caffeoylquinic acid (5-CQA), quercetin, and phloretin with strong antioxidant activity was increased significantly after fermentation. The modified phenolic composition may contribute to the increased antioxidant activity of fermented apple juice. Our findings showed that Lactobacillus plantarum ATCC14917 fermentation may be an efficient way to enhance the bioavailability of phenolic compounds and to protect cells from oxidative stress.


2019 ◽  
Vol 49 (7) ◽  
Author(s):  
Zhihao Dong ◽  
Junfeng Li ◽  
Lei Chen ◽  
Siran Wang ◽  
Tao Shao

ABSTRACT: This study was conducted to evaluate the effects of additives on the fermentation characteristics, chemical composition and in vitro digestibility of tetraploid black locust (TBL). The TBL leaves silage was either untreated (control) or treated with 1 × 106 cfu/g FM Lactobacillus plantarum (L), 1% glucose (G), 3% molasses (M), a combination of 1% glucose and Lactobacillus plantarum (L+G), or a combination of 3% molasses and Lactobacillus plantarum (L+M). Fermentation quality, chemical composition and nutrient digestibility were then analyzed. Ethanol and acetic acid concentrations were the dominant fermentation products in all silages except L+M silage. The L, G and L+G treatments failed to influence the fermentation. The M treatment increased (P<0.05) the lactic acid concentration and lowered (P<0.05) the pH when compared with control silage. The best fermentation properties were observed in L+M silage, as indicated by the dominance of lactic acid over ethanol in fermentation products. The M and L+M silages exhibited higher (P<0.05) dry matter, and M silage showed higher residual water-soluble carbohydrates than the control. Ensiling increased (P<0.05) the in vitro dry matter, neutral detergent fiber and acid detergent fiber degradability of TBL. Among the silages, M silage had the highest levels of dry matter, neutral detergent fiber and acid detergent fiber degradability. The obtained results suggested that application of lactic acid bacteria together with 3% molasses could be an effective strategy to prevent the occurrence of ethanol fermentation and improve fermentation quality of TBL silage; addition of fermentable sugars to TBL improves nutrient availability to ruminants.


2021 ◽  
pp. 1891-1908
Author(s):  
Mikael Neumann ◽  
◽  
Fernando Braga Cristo ◽  
Giovanna Bobato Pontarolo ◽  
Marlon Richard Hilario da Silva ◽  
...  

The objective of this study was to evaluate the efficiency of different types of sealing on the physical losses of dry matter, chemical and fermentation characteristics and in situ digestibility of the dry matter of corn silage stored in the feed out face (ramp) of trench silos. The experimental design used randomized blocks, composed of three treatments, namely: T1 - corn silage preserved with double-sided polyethylene of 110 μm thickness (conventional seal); T2 - corn silage preserved with double-sided polyethylene of 200 μm thickness (double-sided); and T3 - corn silage preserved with oxygen-impermeable film composed of double-sided polyethylene of 80 μm thickness on a translucent vacuum polyamide film of 20 μm thickness (double sealing), with four repetitions each. The use of double-sided polyethylene with 200 μm thickness (double-sided) and oxygen impermeable film composed of double-sided polyethylene and polyamide (double sealing), were effective in preserving the chemical composition, fermentation profile, raw protein composition and fiber quality, in addition, the use of these polymers resulted in greater ruminal digestibility of dry matter and reduced the pH, temperature, temperature gradient and physical losses of corn silage from the feed out face (ramp) of trench silos compared to the conventional sealing.


2018 ◽  
Vol 6 (3) ◽  
pp. 664-671 ◽  
Author(s):  
ERNIATI ERNIATI ◽  
FRANSISKA RUNGKAT ZAKARIA ◽  
ENDANG PRANGDIMURTI ◽  
DEDE ROBIATUL ADAWIYAH ◽  
BAMBANG PONTJO PRIOSOERYANTO ◽  
...  

Dry sheet food products or nori-like products can be produced from the mixture of Gelidium sp. and Ulva lactuca seaweeds, which is called geluring. Making geluring involves heat application that may influence the chemical composition of the product. The goal of this study was to evaluate the chemical characteristics of geluring by measuring proximate composition; dietary fiber, total phenolic, and flavonoid contents; and antioxidant activity(by DPPH analysis)of geluring and compare the values to those of the raw materials. Three types of geluring were prepared following commercial nori preparation procedures with some modifications: P1 (unseasoned), P2 (seasoned), and P3 (seasoned and roasted).The proximate composition of geluring products and raw materials differed significantly(P<0.05). Geluring fiber contents were not significantly different (P>0.05) among P1 (29.19±0.26%), P2 (29.42±0.66%), and P3 (29.83±0.11%), but these values differed significantly (P<0.05) from those of the raw materials. The total phenolic content, flavonoid content, and DPPH activity of P2geluringwere 1.38 mg GAE/g, 1.11 mg QE/g, and 61.23%, respectively, which were significantly (P<0.05) higher than those of P1 and P3 but lower than those of the raw materials. These results suggest that geluring processing might negatively impact the chemical composition of the products, but they still have high antioxidant activity and dietary fiber content and thus have potential for utilization as a functional food product.


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