Accelerated Mice Skin Acute Wound Healing In Vivo by Combined Treatment of Argon and Helium Plasma Needle

2013 ◽  
Vol 44 (3) ◽  
pp. 169-177 ◽  
Author(s):  
Elizabeth García-Alcantara ◽  
Régulo López-Callejas ◽  
Pedro R. Morales-Ramírez ◽  
Rosendo Peña-Eguiluz ◽  
Raúl Fajardo-Muñoz ◽  
...  
Keyword(s):  
Wound Healing ◽  
Combined Treatment ◽  
Helium Plasma ◽  
Plasma Needle ◽  
Acute Wound

scholarly journals Wound Healing: In Vitro and In Vivo Evaluation of a Bio-Functionalized Scaffold Based on Hyaluronic Acid and Platelet-Rich Plasma in Chronic Ulcers

2019 ◽  
Vol 8 (9) ◽  
pp. 1486 ◽  
Author(s):  
Barbara De Angelis ◽  
Margarida Fernandes Lopes Morais D’Autilio ◽  
Fabrizio Orlandi ◽  
Giampiero Pepe ◽  
Simone Garcovich ◽  
...  
Keyword(s):  
Wound Healing ◽  
Hyaluronic Acid ◽  
Platelet Rich Plasma ◽  
Combined Treatment ◽  
Skin Grafting ◽  
Control Group ◽  
In Vivo Evaluation ◽  
Chronic Ulcers

Chronic ulcers are characterized by loss of substance without a normal tendency towards spontaneous healing. The Wound Bed Preparation Guideline advises that after diagnosis, the expert should correct the biological state of the ulcer micro-environment based on TIME principles (Tissue, Infection, Moisture balance, Epidermal). There are many ways to treat such ulcers, for example through use of advanced dressings, negative pressure, surgical toilets, dermal substitutes, autologous skin grafting, and free or local flaps. In vitro and in vivo pre-clinical models hold widely acknowledged potential yet complex limitations. Tissue bioengineering could be an ideal approach to foster innovative strategies in wound healing. Our observational study reports on an in vitro and in vivo evaluation of a bio-functionalized scaffold composed of platelet-rich plasma (PRP) and hyaluronic acid (HA) used in 182 patients affected by chronic ulcers (diabetic and vascular), comparing the results with a control group of 182 patients treated with traditional dressings (HA alone). After 30 days the patients who had undergone the combined treatment (PRP + HA), showed 96.8% ± 1.5% re-epithelialization, as compared to 78.4% ± 4.4% in the control group (HA only). Within 80 days, they had 98.4% ± 1.3% re-epithelialization as compared to 87.8% ± 4.1% in the control group (HA only; p < 0.05). No local recurrence was observed during the follow-up period. PRP + HA treatment showed stronger regenerative potential in terms of epidermal proliferation and dermal renewal compared with HA alone.

scholarly journals Platelets and Pro-Angiogenic Circulating Cells Accelerate Skin Wound Healing

2021 ◽  
Author(s):  
Silvia Erratico ◽  
Marzia Belicchi ◽  
Mirella Meregalli ◽  
Dario Di Silvestre ◽  
Luana Tripodi ◽  
...  
Keyword(s):  
Wound Healing ◽  
Molecular Mechanism ◽  
Platelet Rich Plasma ◽  
Combined Treatment ◽  
Skin Lesions ◽  
Matrix Remodeling ◽  
Skin Wound Healing ◽  
Major Health Problem

Abstract BackgroundDelayed wound healing and chronic skin lesions represent a major health problem. Over the past years, growth factors mediated by platelet-rich plasma (PRP) and cell-based therapies were developed as effective and affordable treatment able to improve wound healing capacity. However, the precise molecular mechanism through which PRP exhibits its beneficial effects remains unrevealed. Herein we show that a combination of PRP and pro-angiogenic cells (AngioPRP) could exert a synergistic positive effect on keratinocyte proliferation and angiogenesis accelerating wound healing.MethodWe designed a sterile and closed class IIa device (NovySep) for single use only to collect blood-derived mononucleated cells and the plasma phase after centrifugation without opening the system. We performed in vitro and in vivo wound healing experiments to assess the angiogenic potential of AngioPRP; we evaluated the extracellular matrix remodeling and the physical properties of treated wounds through mechanical (stress-strain test) and proteomics analysis.ResultAngioPRP enhanced wound healing by promoting uniform regeneration of the basal and granular layers and vessel remodeling. We coupled this effect with normalization of mechanical properties of rescued mouse wounds, which is sustained by a correct arrangement of elastin and collagen fibers. The network analysis-based protein–protein interactions of AngioPRP-treated wounds demonstrated a fingerprint of AngioPRP-related proteins that may provide the signal for a faster wound healing response which include Caveolin and EGFR/TGFβ/β-catenin pathways.ConclusionA combined treatment composed of PRP and a pool of pro-angiogenic/keratogenic cells (AngioPRP) may provide a more integrated method to supports wound healing, by promoting a cascade of events leading to the reduction of TGFβ1/β-catenin up-downstream signaling pathways. The molecular mechanism undergoing the support of AngioPRP to wound healing opens new perspectives in the treatment of skin injuries.

INFLUENCE OF GROWTH REGULATORS AND LED IRRADIATORS ON PINE STRAWBERRY ADAPTATION

1970 ◽  
pp. 12-15
Author(s):  
M. G. Markova ◽  
E. N. Somova
Keyword(s):  
Surface Area ◽  
Growth Regulators ◽  
Leaf Surface ◽  
Combined Treatment ◽  
Fragaria X Ananassa ◽  
Leaf Surface Area ◽  
External Conditions ◽  
Light Effects ◽  
Control Version

Work on going through the adaptation stage of rooted micro-stalks comes down to searching for new growth regulators and studying the influence of external conditions, which include, among other things, light effects. The data of 2018-2019 on the effect of growth regulators Siliplant, EcoFus and experimental LED phytoradiators on the adaptation of rooted micro-stalks of garden strawberries (Fragaria x ananassa duch) in vivo are presented. The object of research is rooted micro-stalks of garden strawberries of the Korona variety. It was revealed that, at the adaptation stage of rooted micro-stalks of strawberries, the most effective was the treatment of plants by spraying with Siliplant at a concentration of 1.0 ml/l and the combined treatment with Siliplant and EcoFus at concentrations of 0.5 ml/l: regardless of lighting, the survival rate averaged 99.4 - 99.7%, the leaf surface area increased significantly from 291.85 mm2 to 334.4 mm2. The number of normally developed leaves of strawberry microplants increased significantly after treatment with all preparations from 3.5 to 6.0, 5.8 and 6.5 pcs/plant, and a significant increase in the height of strawberry rosettes was facilitated by treatment with Siliplant and Siliplant together with EcoFus. Regardless of growth regulators, the most effective was the experimental LED phyto-irradiator with a changing spectrum, which contributed to an increase in leaf surface area, height of rosettes and the number of normally developed leaves in strawberry microplants. When illuminated with a flashing phytoradiator, these indicators are lower than in the control version, but not significantly. By the end of the rooting stage, all microplants of garden strawberries corresponded to GOST R 54051-2010.

In Vivo Wound Healing Activity of Spirulina platensis

2019 ◽  
Vol 18 (1) ◽  
pp. 06-16
Author(s):  
R. Seghiri ◽  
A. Essamri
Keyword(s):  
Wound Healing ◽  
Spirulina Platensis ◽  
Therapeutic Agent ◽  
Conventional Medicine ◽  
Folk Medicine ◽  
Chemical Burns ◽  
Amount Of Substance ◽  
Test Population ◽  
Wound Healing Activity

Spirulina is a microalga used in traditional folk medicine in Morocco for the treatment of various health disorders. The wound healing activity of Moroccan Spirulina is unknown. In the current study, aqueous extracts of Spirulina platensis were investigated for acute toxicity and wound healing activity in Swiss Albino mice and White New Zealand rabbits, respectively. The LD50 (amount of substance required to kill 50% of the test population) of the microalga was greater than 5,000 mg/kg. Healing after application of the same amount of ointment on differently induced (mechanical, chemical, and thermal) wounds was about the same, over five weeks. Aqueous extract had remarkable healing activity on rabbits’ skin, possessing significantly greater healing effect for mechanical and chemical burns than controls. Moreover, the hair growing time was faster in treated groups; Spirulina-treated groups did not show any contamination with microbes compared to others. This study affirms that Spirulina platensis can be considered as a potential therapeutic agent for wound healing not only as a complementary medicine but also in conventional medicine.

Healing effects of curcumin nanoparticles in deep tissue injury mouse model.

2020 ◽  
Vol 18 ◽  
Author(s):  
Zirui Zhang ◽  
Shangcong Han ◽  
Panpan Liu ◽  
Xu Yang ◽  
Jing Han ◽  
...  
Keyword(s):  
Wound Healing ◽  
Mrna Expression ◽  
Tissue Injury ◽  
Inflammatory Cells ◽  
Pcr Analysis ◽  
Protective Effects ◽  
Deep Tissue ◽  
Deep Tissue Injury

Background: Chronic inflammation and lack of angiogenesis are the important pathological mechanisms in deep tissue injury (DTI). Curcumin is a well-known anti-inflammatory and antioxidant agent. However, curcumin is unstable under acidic and alkaline conditions, and can be rapidly metabolized and excreted in the bile, which shortens its bioactivity and efficacy. Objective: This study aimed to prepare curcumin-loaded poly (lactic-co-glycolic acid) nanoparticles (CPNPs) and to elucidate the protective effects and underlying mechanisms of wound healing in DTI models. Methods: CPNPs were evaluated for particle size, biocompatibility, in vitro drug release and their effect on in vivo wound healing. Results : The results of in vivo wound closure analysis revealed that CPNP treatments significantly improved wound contraction rates (p<0.01) at a faster rate than other three treatment groups. H&E staining revealed that CPNP treatments resulted in complete epithelialization and thick granulation tissue formation, whereas control groups resulted in a lack of compact epithelialization and persistence of inflammatory cells within the wound sites. Quantitative real-time PCR analysis showed that treatment with CPNPs suppressed IL-6 and TNF-α mRNA expression, and up-regulated TGF-β, VEGF-A and IL-10 mRNA expression. Western blot analysis showed up-regulated protein expression of TGF-β, VEGF-A and phosphorylatedSTAT3. Conclusion: Our results showed that CPNPs enhanced wound healing in DTI models, through modulation of the JAK2/STAT3 signalling pathway and subsequent upregulation of pro-healing factors.

The Anti-tumor Activity and Mechanisms of rLj-RGD3 on Human Laryngeal Squamous Carcinoma Hep2 Cells

2020 ◽  
Vol 19 (17) ◽  
pp. 2108-2119
Author(s):  
Yang Jin ◽  
Li Lv ◽  
Shu-Xiang Ning ◽  
Ji-Hong Wang ◽  
Rong Xiao
Keyword(s):  
Wound Healing ◽  
Western Blot ◽  
Morphological Changes ◽  
In Vivo Studies ◽  
Migration And Invasion ◽  
Tunel Staining ◽  
Dna Ladder ◽  
Western Blot Assay

Background: Laryngeal Squamous Cell Carcinoma (LSCC) is a malignant epithelial tumor with poor prognosis and its incidence rate increased recently. rLj-RGD3, a recombinant protein cloned from the buccal gland of Lampetra japonica, contains three RGD motifs that could bind to integrins on the tumor cells. Methods: MTT assay was used to detect the inhibitory rate of viability. Giemsa’s staining assay was used to observe the morphological changes of cells. Hoechst 33258 and TUNEL staining assay, DNA ladder assay were used to examine the apoptotic. Western blot assay was applied to detect the change of the integrin signal pathway. Wound-healing assay, migration, and invasion assay were used to detect the mobility of Hep2 cells. H&E staining assay was used to show the arrangement of the Hep2 cells in the solid tumor tissues. Results: In the present study, rLj-RGD3 was shown to inhibit the viability of LSCC Hep2 cells in vitro by inducing apoptosis with an IC50 of 1.23µM. Western blot showed that the apoptosis of Hep2 cells induced by rLj- RGD3 was dependent on the integrin-FAK-Akt pathway. Wound healing, transwells, and western blot assays in vitro showed that rLj-RGD3 suppressed the migration and invasion of Hep2 cells by integrin-FAKpaxillin/ PLC pathway which could also affect the cytoskeleton arrangement in Hep2 cells. In in vivo studies, rLj-RGD3 inhibited the growth, tumor volume, and weight, as well as disturbed the tissue structure of the solid tumors in xenograft models of BALB/c nude mice without reducing their body weights. Conclusion: hese results suggested that rLj-RGD3 is an effective and safe suppressor on the growth and metastasis of LSCC Hep2 cells from both in vitro and in vivo experiments. rLj-RGD3 might be expected to become a novel anti-tumor drug to treat LSCC patients in the near future.

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