Wide range detection of C-Reactive protein with a homogeneous immunofluorimetric assay based on cooperative fluorescence quenching assisted by gold nanoparticles

A series of preparations of gold nanoparticles with diameters from 13 to 60 nm and their conjugates with antibodies (murine immunoglobulins of class G) of different composition were obtained. The composition of the conjugates and the amount of antibodies that retain their reactivity in an immobilized form are characterized. Using the example of immunochromatographic test systems for the detection of D-dimer and C-reactive protein, the effectiveness of conjugates as analytical reagents is compared.

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The Biological Context of C-Reactive Protein as a Prognostic Marker in Renal Cell Carcinoma: Studies on the Acute Phase Cytokine Profile

Cancers ◽

10.3390/cancers12071961 ◽

2020 ◽

Vol 12 (7) ◽

pp. 1961 ◽

Cited By ~ 1

Author(s):

Helene Hersvik Aarstad ◽

Gigja Guðbrandsdottir ◽

Karin M. Hjelle ◽

Leif Bostad ◽

Øystein Bruserud ◽

...

Keyword(s):

Cancer Patients ◽

Renal Cancer ◽

Acute Phase ◽

High Serum ◽

Cytokine Profile ◽

Phase Reaction ◽

C Reactive Protein ◽

Reactive Protein ◽

Wide Range ◽

The Impact

High serum levels of the acute phase protein C-reactive protein (CRP) are associated with an adverse prognosis in renal cancer. The acute phase reaction is cytokine-driven and includes a wide range of inflammatory mediators. This overall profile of the response depends on the inducing event and can also differ between patients. We investigated an extended acute phase cytokine profile for 97 renal cancer patients. Initial studies showed that the serum CRP levels had an expected prognostic association together with tumor size, stage, nuclear grading, and Leibovich score. Interleukin (IL)6 family cytokines, IL1 subfamily mediators, and tumor necrosis factor (TNF)α can all be drivers of the acute phase response. Initial studies suggested that serum IL33Rα (the soluble IL33 receptor α chain) levels were also associated with prognosis, although the impact of IL33Rα is dependent on the overall cytokine profile, including seven IL6 family members (IL6, IL6Rα, gp130, IL27, IL31, CNTF, and OSM), two IL1 subfamily members (IL1RA and IL33Rα), and TNFα. We identified a patient subset characterized by particularly high levels of IL6, IL33Rα, and TNFα alongside an adverse prognosis. Thus, the acute phase cytokine reaction differs between renal cancer patients, and differences in the acute phase cytokine profile are associated with prognosis.

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A comparison study between wide‐range and high‐sensitivity C‐reactive protein assays (Roche Cobas c702) for low C‐reactive protein concentration in patients with cardiovascular risk

Journal of Clinical Laboratory Analysis ◽

10.1002/jcla.22957 ◽

2019 ◽

Vol 33 (8) ◽

Author(s):

Runqing Li ◽

Yajun Xue ◽

Tengjiao Wang ◽

Lijun Gong ◽

Peng Peng ◽

...

Keyword(s):

Cardiovascular Risk ◽

Protein Concentration ◽

High Sensitivity ◽

Comparison Study ◽

C Reactive Protein ◽

Reactive Protein ◽

Wide Range ◽

Protein Assays ◽

Roche Cobas

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Introducing the wide range C-reactive protein (wr-CRP) into clinical use for the detection of microinflammation

Clinica Chimica Acta ◽

10.1016/j.cccn.2005.02.024 ◽

2005 ◽

Vol 358 (1-2) ◽

pp. 151-158 ◽

Cited By ~ 35

Author(s):

Ori Rogowski ◽

Yaffa Vered ◽

Itzhak Shapira ◽

Meirav Hirsh ◽

Vera Zakut ◽

...

Keyword(s):

Clinical Use ◽

C Reactive Protein ◽

Reactive Protein ◽

Wide Range

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Clinical evaluation of point-of-care testing for wide-range C-reactive protein (wr-CRP) in neonates with suspected sepsis

Journal of Laboratory Medicine ◽

10.1515/labmed-2019-0008 ◽

2019 ◽

Vol 43 (3) ◽

pp. 135-140

Author(s):

Amiel Billetop ◽

Kerry Grant ◽

Jennifer Beasmore ◽

Francesca Mills ◽

David Odd ◽

...

Keyword(s):

Operating Characteristic ◽

Point Of Care ◽

Attractive Alternative ◽

C Reactive Protein ◽

Suspected Sepsis ◽

Reactive Protein ◽

Point Of Care Test ◽

Wide Range ◽

The Mean ◽

Common Laboratory

Abstract Background The aim of this study was to validate a point-of-care C-reactive protein (CRP) test (QuikRead, wide-range [wr] CRP) against standard laboratory testing in neonates with suspected sepsis. Methods This was a single-centre prospective cohort study of neonates (n = 91). The main outcome measure was the paired evaluation of the wr-CRP point-of-care test and automated laboratory CRP tests in neonates with suspected sepsis. Results There were 126 measured CRP-sample pairs. The mean difference between the laboratory CRP and the wr-CRP point-of-care test values was 0.19 (95% confidence interval [CI]:‒1.0–0.65). Pearson’s correlation coefficient was 0.94. The area under the receiver operating characteristic (ROC) curve was 0.99 (95% CI: 0.98–1.00). At a QuikRead CRP cut-off of ≥6.2, the sensitivity and specificity were 77% and 100%, respectively. Conclusions Point-of-care wr-CRP testing can be used as a screening test in neonates with suspected sepsis. Rapid bed-side diagnostics and minimal blood volume requirements present an attractive alternative to common laboratory CRP testing.

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Serial Measurements of C-Reactive Protein and Interleukin-6 in the Immediate Postnatal Period: Reference Intervals and Analysis of Maternal and Perinatal Confounders

Clinical Chemistry ◽

10.1093/clinchem/47.6.1016 ◽

2001 ◽

Vol 47 (6) ◽

pp. 1016-1022 ◽

Cited By ~ 66

Author(s):

Claudio Chiesa ◽

Fabrizio Signore ◽

Marcello Assumma ◽

Elsa Buffone ◽

Paola Tramontozzi ◽

...

Keyword(s):

Interleukin 6 ◽

Early Onset ◽

Gestational Hypertension ◽

Neonatal Infection ◽

Reference Intervals ◽

Postnatal Period ◽

C Reactive Protein ◽

Reactive Protein ◽

Wide Range ◽

Near Term

Abstract Background: There is a wide range of reported sensitivities and specificities for C-reactive protein (CRP) and interleukin-6 (IL-6) in the detection of early-onset neonatal infection. This prompted us to assess reference intervals for CRP and IL-6 during the 48-h period immediately after birth and to identify maternal and perinatal factors that may affect them. Methods: CRP and IL-6 values were prospectively obtained for 148 healthy babies (113 term, 35 near-term) at birth and at 24 and 48 h of life, and from their mothers at delivery. Results: Upper reference limits for CRP at each neonatal age were established. At birth, CRP was significantly lower than at 24 and 48 h of life. Rupture of membranes ≥18 h, perinatal distress, and gestational hypertension significantly affected the neonatal CRP dynamics, but at specific ages. There was no correlation between CRP concentrations in mothers and their offspring at birth. The IL-6 values observed in the delivering mothers and in their babies at all three neonatal ages were negatively associated with gestational age. In the immediate postnatal period, IL-6 dynamics for term babies were significantly different from those for near-term babies. Maternal IL-6 concentrations correlated with babies’ IL-6 concentrations only for term deliveries. Apgar score had a significant effect on babies’ IL-6 values at birth. Conclusions: The patterns of CRP and IL-6 responses in the healthy neonate should be taken into account to optimize their use in the diagnosis of early-onset neonatal sepsis.

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Comparative Study of Serum Amyloid A Protein and C-Reactive Protein in Disease

Clinical Science ◽

10.1042/cs0680233 ◽

1985 ◽

Vol 68 (2) ◽

pp. 233-238 ◽

Cited By ~ 82

Author(s):

C. P. J. Maury

Keyword(s):

Serum Amyloid A ◽

Tissue Injury ◽

Serum Amyloid ◽

Response To Treatment ◽

Surgical Trauma ◽

C Reactive Protein ◽

Reactive Protein ◽

Amyloid A ◽

Wide Range ◽

Serum Amyloid A Protein

1. On the basis of results from 3000 parallel measurements of serum amyloid A protein (SAA) and C-reactive protein (CRP) in various clinical and experimental conditions, the relationship between these proteins was examined and the question of whether measurements of SAA can provide clinically useful information beyond that from CRP assays was evaluated. 2. The concentrations of SAA and CRP showed a close relationship in a wide range of clinical conditions and the general clinical impact of an elevated SAA or CRP level is similar. SAA was, however, more sensitive than CRP in reflecting inflammatory activity, and in some conditions characterized by normal or only slightly elevated CRP concentrations, measurements of SAA concentrations could be used for monitoring disease activity and response to treatment. 3. Marked variation in the ratios of SAA to CRP concentration occurred in response to different stimuli (e.g. surgical trauma/immunological tissue injury), suggesting the existence of independent, disease-specific pathways of regulation for the serum concentrations of SAA and CRP.

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Localized Surface Plasmon Resonance Nanosensing of C-Reactive Protein with Poly(2-methacryloyloxyethyl phosphorylcholine)-Grafted Gold Nanoparticles Prepared by Surface-Initiated Atom Transfer Radical Polymerization

Analytical Chemistry ◽

10.1021/ac501322x ◽

2014 ◽

Vol 86 (11) ◽

pp. 5587-5594 ◽

Cited By ~ 50

Author(s):

Yukiya Kitayama ◽

Toshifumi Takeuchi

Keyword(s):

Gold Nanoparticles ◽

Surface Plasmon Resonance ◽

Atom Transfer Radical Polymerization ◽

Radical Polymerization ◽

Surface Plasmon ◽

Plasmon Resonance ◽

Localized Surface Plasmon Resonance ◽

Localized Surface Plasmon ◽

C Reactive Protein ◽

Reactive Protein

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Insulin Resistance Is Associated With Enhanced Brain Glucose Uptake During Euglycemic Hyperinsulinemia: A Large-Scale PET Cohort

10.2337/figshare.13342142.v1 ◽

2021 ◽

Author(s):

Eleni Rebelos ◽

Marco Bucci ◽

Tomi Karjalainen ◽

Vesa Oikonen ◽

Alessandra Bertoldo ◽

...

Keyword(s):

Insulin Resistance ◽

Type 2 Diabetes ◽

Fatty Acids ◽

Insulin Sensitivity ◽

Glucose Uptake ◽

C Reactive Protein ◽

Brain Glucose ◽

Reactive Protein ◽

Wide Range

Objective Whereas insulin resistance is expressed as reduced glucose uptake in peripheral tissues, the relationship between insulin resistance and brain glucose metabolism remains controversial. Our aim was to examine the association of insulin resistance and brain glucose uptake (BGU) during a euglycemic hyperinsulinemic clamp in a large sample of subjects across a wide range of age and insulin sensitivity. Research Design and Methods [18F]-fluorodeoxyglucose positron emission tomography (PET) data from 194 subjects scanned under clamp conditions were compiled from a single-center cohort. BGU was quantified by the fractional uptake rate. We examined the association of age, sex, M value from the clamp, steady-state insulin and free fatty acids levels, C-reactive protein, HbA1c, and presence of type 2 diabetes with BGU using Bayesian hierarchical modeling. Results Insulin sensitivity, indexed by the M value, was associated negatively with BGU in all brain regions, confirming that in insulin resistant subjects BGU is enhanced during euglycemic hyperinsulinemia. In addition, the presence of type 2 diabetes was associated with a further increase in BGU. On the contrary, age was negatively related to BGU. Steady-state insulin levels, C-reactive protein, free fatty acids, sex, and HbA1c were not associated with BGU. Conclusions In this large cohort of subjects of either sex across a wide range of age and insulin sensitivity, insulin sensitivity is the best predictor of brain glucose uptake.

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