Variability of qualitative and quantitative secondary metabolites traits among wild genetic resources of Lavandula stoechas L.

2021 ◽  
Vol 98 ◽  
pp. 104327
Author(s):  
Hnia Chograni ◽  
Leila Riahi ◽  
Chokri Messaoud
Keyword(s):  
Secondary Metabolites ◽  
Genetic Resources ◽  
Qualitative And Quantitative ◽  
Lavandula Stoechas

scholarly journals PHYTOCHEMICAL CONSTITUENTS OF THE ROOT OF BERBERIS MICROPHYLLA

2017 ◽  
Vol 10 (6) ◽  
pp. 225 ◽  
Author(s):  
María Cristina Furrianca ◽  
Marysol Alvear ◽  
Tomás Zambrano ◽  
Víctor Fajardo ◽  
Luis Salazar
Keyword(s):  
Secondary Metabolites ◽  
Cardiac Glycosides ◽  
Methanolic Extract ◽  
Ethanolic Extract ◽  
Phytochemical Screening ◽  
Qualitative And Quantitative ◽  
Medicinal Properties ◽  
Phytochemical Constituents ◽  
First Time

Objective: To objective of this work was to perform phytochemical qualitative and quantitative analyzes of the main secondary metabolites in the root of Berberis microphylla.Methods: The extracts of B. microphylla root were tested through phytochemical screening and the quantification of the most important constituents was carried out using spectrophotometric and gravimetric techniques.Results: Phytochemical screening of both extracts showed the presence of alkaloids, flavonoids, glycosides, cardiac glycosides, saponins, terpenes,and tannins, which are pharmacologically important. Quantification of the major phytochemicals groups showed that the ethanolic extract contains 3.9% alkaloids, 0.46% flavonoids, 9.53% tannins, and 3.60% saponins. Similarly, the methanolic extract contains 6.61% alkaloids, 0.41% flavonoids, 7.40% tannins, and 1.43% saponins.Conclusion: This is the first time that the presence of tannins, flavonoids, and saponins in this plant has been reported. The medicinal properties of the root of B. microphylla may exist due to the presence secondary metabolites.

scholarly journals Qualitative and quantitative evaluation of the phytochemical constituents of three wood species in Ogun state, Nigeria

2020 ◽  
Vol 7 (3) ◽  
pp. 627-633
Author(s):  
K. M. Ogunjobi ◽  
◽  
S. O. Abdulwahab ◽  
O. F. Gakenou ◽  
O. E. Thompson ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Plant Protection ◽  
Research Work ◽  
Tectona Grandis ◽  
Gmelina Arborea ◽  
Stem Wood ◽  
Qualitative And Quantitative ◽  
Ogun State ◽  
Phytochemical Constituents ◽  
Anogeissus Leiocarpus

Studies on the phytochemicals of the stem wood of tropical trees are scarce, despite its importance to plant protection and preservation as most researches focused on their leaves and fruits. This research work aimed to qualitatively and quantitatively analyze the phytochemicals present in the stem wood of Gmelina arborea, Tectona grandis and Anogeissus leiocarpus. Freshly sawn timbers were collected from a local sawmill and then grounded into finely powdered wood samples. The powdered wood samples and its extracts were screened for the presence or absence of phytochemicals using standard methodologies. The qualitative screening revealed the presence of various secondary metabolites such as tannin, saponin, steroids, flavonoid, alkaoids and terpene in all the three species. The result also showed that Tectona grandis had the highest percentage of Alkaloid (7.5%), Tannin (4.95%), and Flavonoid (4.67%) while Anogeissus leiocarpus had the highest percentage of Saponin (3.06%) and Terpene (1.45%). This study established the fact that the three selected species studied have potentials in the industries for medicinal and anti-pathogenic usages.

Biodiversity of carrot genetic resources – variation in secondary metabolites

Planta Medica ◽  
2011 ◽  
Vol 77 (12) ◽  
Author(s):  
R Baranski ◽  
C Allender ◽  
I Kaminska ◽  
M Jemiola Rzeminska
Keyword(s):  
Secondary Metabolites ◽  
Genetic Resources

scholarly journals The Slovak gene pool of German chamomile (Matricaria recutita L.) and comparison in its parameters

2011 ◽  
Vol 31 (No. 2) ◽  
pp. 70-75 ◽  
Author(s):  
I. Šalamon
Keyword(s):  
Secondary Metabolites ◽  
Genetic Variability ◽  
Essential Oil ◽  
Gene Pool ◽  
Qualitative And Quantitative ◽  
Quantitative Characteristics ◽  
Environmental Variations ◽  
Exogenous Factors ◽  
Plant Habit ◽  
Qualitative And Quantitative Characteristics

Plant habit and production of secondary metabolites in chamomile plants depend on endogenous and exogenous factors that can be divided into two groups (Franz 1982): a) morpho-ontogenetic variability, b) genetic variability or genetic determinateness. The activity of these factors is reflected in biomass production, flower drug production, content and composition of essential oil and some other characteristics of chamomile stands. This study presents genetic and environmental variations of essential oil and its sesquiterpene composition – (-)-B of chamomile population (Lawrence 1986). Qualitative and quantitative characteristics of the chamomile essential oil of selected cultivars indicate (-)- compounds belong to the chemotype C group (Lawrence 1986). Parameters of the composition of essential oil from cultivated chamomile varieties,

scholarly journals QUALITATIVE AND QUANTITATIVE PHYTOCHEMICALANALYSIS AND DPPH RADICAL SCAVENGING ACTIVITY OF DIFFERENT EXTRACTSOF FLOWER OF BAUHINIA ACUMINATA LINN

2017 ◽  
Vol 9 (6) ◽  
pp. 23
Author(s):  
Sreeja Sanjeev ◽  
Regi Raphael K.
Keyword(s):  
Secondary Metabolites ◽  
Skin Diseases ◽  
Quantitative Estimation ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Dependent Manner ◽  
Scavenging Activity ◽  
Primary And Secondary Metabolites ◽  
Qualitative And Quantitative

Objective: Bauhinia acuminate L (Fabaceae) is a species of flowering shrub native to tropical south eastern Asia. The flowerof B. acuminataare used against various skin diseases,worms, tumours and diabetes. The Indian vaiydas recommended as a remedy to treat severe biliousness.The qualitative and quantitative estimation of phytochemical constituents in various extracts and the antioxidant potential of flower of Bauhinia acuminata were evaluated.Methods: All the analysis was done based on standard protocols.Results: The phytochemical screening reveals the presence of various primary and secondary metabolites like sugar, carbohydrate, amino acid, fat, quinone, steroids, phenol, saponin, alkaloids, and acid content. In quantitative analysis the important secondary metabolites such as alkaloids and phenolic compounds were estimated in all the extracts.The ethanol and chloroformextract produced significant antioxidant properties in a dose-dependent manner. DPPH free radical scavenging assay of ethanol and chloroform extracts of flower exhibited IC 50 values of 24.44±1.201μg/ml and 196.68±0.456μg/ml respectively. At 1000 μg/ml concentration both extracts shows maximum radical scavenging activity (98.97% and 85.67 %).Conclusion: The findings of this study indicate that this plant is medicinal with prominent antioxidant property.

scholarly journals Selection of an optimal method for screening the collection of narrow-leaved lupine held by the Vavilov Institute for the qualitative and quantitative composition of seed alkaloids

2020 ◽  
Vol 24 (8) ◽  
pp. 829-835
Author(s):  
A. V. Kushnareva ◽  
Т. V. Shelengа ◽  
I. N. Perchuk ◽  
G. P. Egorova ◽  
L. L. Malyshev ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Genetic Resources ◽  
Gene Pool ◽  
Plant Genetic Resources ◽  
Alkaloid Content ◽  
Gas Chromatography Mass Spectrometry ◽  
Quantitative Composition ◽  
Qualitative And Quantitative ◽  
Vavilov Institute ◽  
Selection Of

Narrow-leaved lupine (Lupinus аngustifolius L.) is a widely cultivated leguminous forage and green manure crop with a potential for human nutrition. However, the presence of secondary metabolites – alkaloids – in lupine seeds considerably affects the quality of raw produce, reducing its nutritive value; in addition, high concentrations of alkaloids are toxic to humans and animals. Therefore, plant breeders working with lupine need to gain knowledge about the variability of alkaloid content in seeds of different genotypes and search for the sources of their low concentrations in the crop’s gene pool. The collection of narrow-leaved lupine genetic resources held by the N.I. Vavilov Institute of Plant Genetic Resources (VIR) offers wide opportunities for such search by means of mass screening. For its part, largescale gene pool screening requires the selection of an optimal technique to measure alkaloid content in seeds, so that it would be easily reproducible and as little labor-, time- and fund-consuming as possible. The results of the search for such method are presented. Qualitative and quantitative indices were compared when target compounds had been extracted with multicomponent mixtures and individual reagents (chloroform, methanol, etc.) and the extracts analyzed using gas chromatography-mass spectrometry. High-performance liquid chromatography combined with mass spectrometry was also employed. Five major alkaloids were found to be present in all types of extracts: lupanine, 13-hydroxylupanine (dominant ones), angustifoline, sparteine, and isolupanine. The fullest extraction of alkaloids was observed when the extractant with an added alkaline agent was used (425 mg/100 g). The lowest level of extraction was registered with chloroform (216 mg/100 g). The significance of the differences was confirmed statistically.

scholarly journals COMPARATIVE ANALYSIS OF QUALITATIVE AND QUANTITATIVE COMPOSITION IN FLAVONOIDS PHYSCIA STELLARIS (L.) NYL WHO GREW UP IN PLACES OF DIFFERENT ANTHROPOGENIC LOAD

2017 ◽  
pp. 153-158
Author(s):  
Рашит (Rashit) Габдулхаевич (Gabdulhaevich) Фархутдинов (Farkhutdinov) ◽  
Аркадий (Arkadij) Владимирович (Vladimirovich) Щербаков (Shcherbakov) ◽  
Зиля (Zilja) Равиловна (Ravilovna) Саитова (Saitova)
Keyword(s):  
Comparative Analysis ◽  
Secondary Metabolites ◽  
Antioxidant Status ◽  
Growth Response ◽  
Anthropogenic Load ◽  
Quantitative Composition ◽  
Flavonoid Content ◽  
Qualitative And Quantitative ◽  
Phenolic Substances ◽  
Different Levels

The studies was defined qualitative and quantitative composition of flavonoids in lichen species Physcia stellaris (L.) Nyl., Collected in habitats with different levels of anthropogenic load. Changes in the composition of flavonoids is considered in the literature as an indicator of the direction of the synthesis of secondary metabolites by plants to protect against adverse environmental influences. It was found that the most diverse number of members (128 compounds), phenolic substances found in the least contaminated RB Ishimbai area and the fewest (58 compounds) was detected in Sterlitamak. Analysis of qualitative and quantitative composition of flavonoids showed that in the area of Ishimbaisky samples were found to accumulate naringin, dihydroquercetin, quercetin. The samples obtained in Alsheyevsky District noted in content highs routine, but samples Sterlitamak – fizetina and naringenin. The calculation of the potential antioxidant status showed that the samples of Ishimbaisky district formed the highest level of protection phenolic substances. It showed no direct correlation between the growth response to environmental pollution and the level of flavonoid content in the thallus of lichens.

scholarly journals Spatial separation of Streptomyces aerial mycelium during fermentation enhances secondary metabolite production

2020 ◽  
Author(s):  
Balaji Muralikrishnan ◽  
Retnakumar R J ◽  
Ranjit Ramachandran ◽  
Vinodh J S ◽  
Nijisha M ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Secondary Metabolite ◽  
Aerial Mycelium ◽  
Spatial Separation ◽  
Bioactive Molecules ◽  
Secondary Metabolite Production ◽  
Effective Technique ◽  
Batch Cultures ◽  
Metabolite Production ◽  
Qualitative And Quantitative

AbstractConditioning of morphology is an effective technique to enhance secondary metabolite production by Streptomyces. Here we report a novel conditioning method employing glass marbles in batch cultures to enhance secondary metabolite production by Streptomyces sp. The marbles seem to spatially separate aerial and submerged mycelia in the flask which was necessary for the qualitative and quantitative enhancement of metabolite production of secondary metabolites. The method also offers shorter incubation period compared to conventional methods for effective production. Further, using a combination of this method and response surface methodology we could enhance the production of antimycobacterial molecules chrysomycin A and B significantly.ImportanceRediscovery of existing molecules and lack of techniques to induce production of secondary metabolites are the major bottlenecks associated with drug discovery of novel bioactive molecules from Streptomyces, the major source of marketable drugs today. We found a new method to increase the diversity and quantity of secondary metabolites in two Streptomyces species. This method thus enhances the chance of finding novel active principles from Streptomyces.

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