Novel hexahydrocannabinol analogs as potential anti-cancer agents inhibit cell proliferation and tumor angiogenesis

2011 ◽  
Vol 650 (1) ◽  
pp. 64-71 ◽  
Author(s):  
Dinesh Thapa ◽  
Jong Suk Lee ◽  
Se-Woong Heo ◽  
Yong Rok Lee ◽  
Keon Wook Kang ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Tumor Angiogenesis ◽  
Anti Cancer ◽  
Inhibit Cell Proliferation

scholarly journals Prostate Cancer-Specific of DD3-driven oncolytic virus-harboring mK5 gene

Open Medicine ◽  
2018 ◽  
Vol 14 (1) ◽  
pp. 1-9
Author(s):  
Jiali Hao ◽  
Wenjie Xie ◽  
Hui Li ◽  
Runsheng Li
Keyword(s):  
Prostate Cancer ◽  
Cell Proliferation ◽  
Differential Display ◽  
Tumor Angiogenesis ◽  
Oncolytic Virus ◽  
Antitumor Effect ◽  
Oncolytic Adenovirus ◽  
Male Population ◽  
Human Plasminogen ◽  
Inhibit Cell Proliferation

AbstractProstate cancer (PCa) is the second most diagnosed cancer in Western male population. In this study, we insert mK5 (the mutational kringle5 of human plasminogen) into a DD3-promoted (differential display code 3) oncolytic adenovirus to construct OncoAd.mK5.DD3. E1A.dE1B, briefly, OAd.DD3.mK5. DD3 is one of the most prostate cancer specific promoters which can transcriptionally control adenoviral replication. mK5 has been proved to be able to inhibit the tumor angiogenesis and inhibit cell proliferation. Our results suggested that targeting PCa with OAd.DD3.mK5 elicited strong antitumor effect.

Adhesion barriers inhibit cell proliferation, adhesion and invasion of primary endometriosis cells

2014 ◽  
Vol 74 (S 01) ◽  
Author(s):  
SP Renner ◽  
MW Beckmann ◽  
S Burghaus ◽  
R Strick ◽  
PL Strissel ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Adhesion And Invasion ◽  
Inhibit Cell Proliferation

Polyamide Amine Dendrimer Nanoparticles Conjugated with Cis-Diamminedichloroplatinum (CDDP) and miRNA-200c Inhibitor Suppresses Lung Cancer Cell Proliferation

2021 ◽  
Vol 11 (9) ◽  
pp. 1760-1768
Author(s):  
Fang Zhang ◽  
Jili Zou ◽  
Dandan Huang
Keyword(s):  
Lung Cancer ◽  
Cell Proliferation ◽  
Cancer Cell Proliferation ◽  
Lung Cancer Cell ◽  
Targeting Delivery ◽  
Apoptosis Related Protein ◽  
Related Proteins ◽  
Exclusion Method ◽  
Inhibit Cell Proliferation ◽  
Excellent Stability

Our study elucidates the effect of folate polyamide amine dendrimer nanoparticles targeting delivery of miRNA-200c inhibitor and CDDP on lung cancer cells proliferation. We established polyamide amine dendrimer nanoparticles binding with CDDP and miRNA-200c inhibitor (Den-PEI-CDDP-siRNA-FA), TEM was employed to detect the morphology of nanoparticles. Agarose gel assay was selected for stabilization test. Cell proliferation were detected by trypanosoma blue exclusion method. The expression of miRNA-200c targeted APKPA12 and apoptosis-related proteins were detected by Western blot and PCR. Finally, apoptosis was analyzed by flow cytometry. Den-PEI-CDDP-siRNA-FA nanoparticles showed excellent stability and drug encapsulation ability. Nanoparticles targeting for FRA to co-deliver siRNA and CDDP could significantly promote cell apoptosis, increase apoptosis-related protein expression, and inhibit cell proliferation. Besides, nanoparticles exerted less venomous effect than untargeted nanoparticles in MRC9 lung fibroblast. Den nanoparticle targeting FRA might be used as the carrier for joint applications with siRNA and CDDP for treating lung cancer.

scholarly journals Sulforaphane suppresses autophagy during the malignant progression of gastric carcinoma via activating miR-4521/PIK3R3 pathway

2021 ◽  
pp. 096032712110544
Author(s):  
Zi-tan Peng ◽  
Pei Gu
Keyword(s):  
Cell Proliferation ◽  
Gastric Carcinoma ◽  
Carcinoma Cells ◽  
Malignant Progression ◽  
Gastric Carcinoma Cell ◽  
Over Expression ◽  
Anti Cancer ◽  
Underlying Mechanisms ◽  
Cruciferous Plants

Objective Sulforaphane, which exerts an effective anti-cancer ability, is a phytochemical converted from cruciferous plants. Here, we aimed to identify whether sulforaphane could suppress autophagy during the malignant progression of gastric carcinoma and to explore the underlying mechanisms. Methods SGC7901 cells were transfected with miR-4521 mimics, inhibitor, and pcDNA3.1- PIK3R3, and treated with sulforaphane or autophagy inhibitor. Cell proliferation, apoptosis, and miR-4521 or PIK3R3 expression were detected. Results MiR-4521 over-expression suppressed LC3-II/I ratio and Beclin-1 expression but induced p62 expression in SGC7901 cells. MiR-4521 also reduced gastric carcinoma cell proliferation and promoted apoptosis in vitro. In the mechanical observation, we identified that miR-4521 directly targeted PIK3R3 to repress its expression, and PIK3R3 up-regulation partly antagonized miR-4521-mediated autophagy, proliferation, and apoptosis in gastric carcinoma cells. In addition, sulforaphane exerted effective anti-cancer functions by repressing autophagy and growth in tumor cells at a concentration-dependent way. MiR-4521 inhibition or PIK3R3 over-expression weakened the anti-cancer functions of sulforaphane in gastric carcinoma cells. Conclusion Consequently, miR-4521 suppressed autophagy during the malignant progression of gastric carcinoma by targeting PIK3R3. Thus, miR-4521 may be applied as a therapeutic target for sulforaphane in gastric carcinoma.

scholarly journals Clinical significance of activated Wnt/β-catenin signaling in apoptosis inhibition of oral cancer

2021 ◽  
Vol 16 (1) ◽  
pp. 1045-1052
Author(s):  
Yufeng Wang ◽  
Zheng Cao ◽  
Fengjia Liu ◽  
Yuejian Ou
Keyword(s):  
Cell Proliferation ◽  
Signaling Pathway ◽  
Apoptotic Cell ◽  
Cancer Cell Proliferation ◽  
Rt Pcr ◽  
Sirna Transfection ◽  
Apoptosis Resistance ◽  
Apoptosis Inhibition ◽  
Anti Cancer ◽  
Evolutionarily Conserved

Abstract Wnt/β‐catenin signaling is an evolutionarily conserved pathway and plays a crucial role in regulating cancer cell proliferation and tumorigenesis. However, the molecular mechanism behind the Wnt/β‐catenin signaling-mediated carcinogenesis and apoptosis resistance in oral squamous cell carcinoma is not well characterized so far. In the present study, we have investigated the effect of β‐catenin depletion of the perversely activated Wnt/β-catenin signaling pathway on apoptosis resistance and tumorigenesis of the human OSCC cell line SCC-55. RT-PCR and western blot analysis demonstrated that the Wnt/β-catenin signaling pathway and its downstream targets such as DKK1 and AXIN2 are aberrantly activated in SCC-55 cells. Furthermore, upon silencing (RNA interference) of β‐catenin in SCC-55, cells became more sensitive toward the chemotherapeutic drugs and thus resulted in apoptotic cell death. Meanwhile, flow cytometry analysis confirmed the enhanced apoptosis and activation of caspases in β‐catenin RNAi cells. Besides ensuing β-catenin–siRNA transfection, the cell proliferation and cancer colony generating efficiencies are significantly impeded compared to the non-transfected cells. Furthermore, the tumorigenicity was inhibited by the downregulation of OCT-4 in β‐catenin-silenced SCC-55 cells. Altogether, Wnt/β‐catenin signaling could potentially target anti-cancer drugs to induce apoptosis and achieve a better clinical outcome.

scholarly journals Role of Antioxidant Vitamins in Neurogenesis

2021 ◽  
Vol 15 (1) ◽  
pp. 11
Author(s):  
Shreyashish Roy-Chowdhury ◽  
Daivat Bhavsar ◽  
Jasrita Singh ◽  
Austin Mardon
Keyword(s):  
Cell Proliferation ◽  
Neural Differentiation ◽  
Scientific Evidence ◽  
Antioxidant Vitamins ◽  
Antioxidant Vitamin ◽  
Regulatory Factor ◽  
Functional Aspects ◽  
Cycle Regulation ◽  
Inhibit Cell Proliferation

Neurogenesis is vital in the preservation of cognition. Previous studies have reported antioxidant vitamins as a key regulatory factor in neurogenesis. However, current research investigating their role is inconclusive due to the limited number of studies that have been conducted and conflicting results. This review evaluates the scientific evidence behind the potential roles of antioxidant vitamins in neurogenesis. Observations concerned with the mechanistic and functional aspects of how antioxidant vitamins modulate neurogenesis are both assessed. Vitamin A is evidently involved in cell cycle regulation and cell proliferation; vitamin C reportedly promotes neural differentiation and maturation while inhibiting neurite outgrowth; vitamin E is identified to inhibit cell proliferation while improving cell viability. Varying antioxidant vitamin concentrations have been implicated in facilitating cognition in terms of attention, memory, language, and executive function. Moreover, this review suggests a threshold antioxidant vitamin concentration that should be maintained to promote optimal levels of adult neurogenesis.

scholarly journals RNA-guided cell targeting with CRISPR/RfxCas13d collateral activity in human cells

2021 ◽  
Author(s):  
Peiguo Shi ◽  
Michael R Murphy ◽  
Alexis O Aparicio ◽  
Jordan S Kesner ◽  
Zhou Fang ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Single Cell ◽  
State Transition ◽  
Cell Types ◽  
Rapid Identification ◽  
Human Cells ◽  
Single Cell Sequencing ◽  
Functional Studies ◽  
Single Marker ◽  
Inhibit Cell Proliferation

While single-cell sequencing has allowed rapid identification of novel cell types or states and associated RNA markers, functional studies remain challenging due to the lack of tools that are able to target specific cells based on these markers. Here we show that targeting a single marker RNA with CRISPR/RfxCas13d led to collateral transcriptome destruction in human cells, which can be harnessed to inhibit cell proliferation or to suppress cell state transition.

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