Antioxidant activity of ethanolic extract of heteropterys aphrodisiaca, an endemic Brazilian plant - trolox equivalent antioxidant capacity assay (TEAC)

Sudarshanapowder (SP) is one of the most effective Ayurveda powder preparations for paediatric febrile conditions. The objective of the present study was to evaluate thein vitroandin vivoantioxidant potentials of SP. Thein vitroantioxidant effects were evaluated using ABTS radical cation decolourization assay where the TROLOX equivalent antioxidant capacity (TEAC) was determined. Thein vivoantioxidant activity of SP was determined in Wistar rats using the Lipid Peroxidation (LPO) assay in serum. Thein vitroassay was referred to as the TROLOX equivalent antioxidant capacity (TEAC) assay. For thein vivoassay, animals were dosed for 21 consecutive days and blood was drawn to evaluate the MDA level. Thein vitroantioxidant activity of 0.5 μg of SP was equivalent to 14.45 μg of standard TROLOX. The percentage inhibition against the radical formation was50.93±0.53%. The SP showed a statistically significant (p<0.01) decrease in the serum level of thiobarbituric acid-reactive substance in the test rats when compared with the control group. These findings suggest that the SP possesses potent antioxidant activity which may be responsible for some of its reported bioactivities.

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Potential of Baccharis alnifolia Meyen & Walpan (Chilka) from northern Chile used as a medicinal infusion

Ciência Rural ◽

10.1590/0103-8478cr20190428 ◽

2019 ◽

Vol 49 (10) ◽

Cited By ~ 2

Author(s):

Emilio Soto ◽

Luis Bustos ◽

Mario Juan Simirgiotis ◽

Claudio Parra

Keyword(s):

Antioxidant Capacity ◽

Ethanolic Extract ◽

Ethanol Extract ◽

Dry Weight ◽

Trolox Equivalent Antioxidant Capacity ◽

Antioxidant Power ◽

Trolox Equivalent ◽

Ferric Reducing Antioxidant Power ◽

Chilean Altiplano ◽

First Time

ABSTRACT: Baccharis alnifolia Meyen & Walp. is a native and medicinal, Chilean Altiplano herb that is used as a poultice and also consumed as an infusion to relieve inflammation. This study evaluated the antioxidant and cytotoxic effect of the ethanolic extract of B. alnifolia and their infusion. It was reported that the ethanol extract contains 4.42 mg GAE/g of dry weight and the infusion contains 35.86 mg GAE/L of total polyphenols. Also, it determined the antioxidant capacity using Ferric reducing antioxidant power (FRAP), Oxygen Radical Absorbance Capacity (ORAC) and Trolox equivalent antioxidant capacity (TEAC) assay. Moreover, seven metabolites including between phenolic acids and flavonoids were identified for the first time in both extracts of the medicinal plant Baccharis alnifolia. The UHPLC-DAD chromatograms revealed the majority presence of ferulic acid and luteolin, both could be responsible for the antioxidant and cytotoxic activity. Furthermore, we realized a preliminary screening of cytotoxicity in different tumor cell lines, finding that these extracts have cytotoxic potential on kidney cells.

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Quantification of the Antioxidant Activity of Plant Extracts: Analysis of Sensitivity and Hierarchization Based on the Method Used

Antioxidants ◽

10.3390/antiox9010076 ◽

2020 ◽

Vol 9 (1) ◽

pp. 76 ◽

Cited By ~ 3

Author(s):

Natividad Chaves ◽

Antonio Santiago ◽

Juan Carlos Alías

Keyword(s):

Antioxidant Activity ◽

Antioxidant Capacity ◽

Plant Species ◽

Radical Scavenging ◽

Reducing Power ◽

Trolox Equivalent Antioxidant Capacity ◽

Antioxidant Compounds ◽

Frap Assay ◽

Trolox Equivalent

Plants have a large number of bioactive compounds with high antioxidant activity. Studies for the determination of the antioxidant activity of different plant species could contribute to revealing the value of these species as a source of new antioxidant compounds. There is a large variety of in vitro methods to quantify antioxidant activity, and it is important to select the proper method to determine which species have the highest antioxidant activity. The aim of this work was to verify whether different methods show the same sensitivity and/or capacity to discriminate the antioxidant activity of the extract of different plant species. To that end, we selected 12 species with different content of phenolic compounds. Their extracts were analyzed using the following methods: 2,2-di-phenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity assay, ferric reducing (FRAP) assay, Trolox equivalent antioxidant capacity (ABTS) assay, and reducing power (RP) assay. The four methods selected could quantify the antioxidant capacity of the 12 study species, although there were differences between them. The antioxidant activity values quantified through DPPH and RP were higher than the ones obtained by ABTS and FRAP, and these values varied among species. Thus, the hierarchization or categorization of these species was different depending on the method used. Another difference established between these methods was the sensitivity obtained with each of them. A cluster revealed that RP established the largest number of groups at the shortest distance from the root. Therefore, as it showed the best discrimination of differences and/or similarities between species, RP is considered in this study as the one with the highest sensitivity among the four studied methods. On the other hand, ABTS showed the lowest sensitivity. These results show the importance of selecting the proper antioxidant activity quantification method for establishing a ranking of species based on this parameter.

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Impact of Bovine Lipocalin-2 Gene on the Antioxidant Activity of Milk from Polish Holstein-Friesian Cows

Animals ◽

10.3390/ani9110992 ◽

2019 ◽

Vol 9 (11) ◽

pp. 992 ◽

Cited By ~ 1

Author(s):

Joanna Pokorska ◽

Dominika Kułaj ◽

Agata Piestrzyńska-Kajtoch ◽

Anna Radko

Keyword(s):

Antioxidant Activity ◽

Antioxidant Capacity ◽

Antioxidant Properties ◽

Trolox Equivalent Antioxidant Capacity ◽

Lipocalin 2 ◽

Holstein Friesian ◽

Total Antioxidant ◽

Trolox Equivalent ◽

Daily Milk Yield ◽

Friesian Cows

In the recent years, antioxidant properties of food products have become an important aspect for consumers. Milk is a very good source of easily absorbable proteins and minerals, as well as a valuable source of antioxidants. Lipocalin-2 (LCN2), given that, inter alia, it is produced in large quantities by various types of cells in response to oxidative stress caused by physical or chemical factors, it can be considered a protein that determines the total antioxidant capacity of milk. The main objective of this study was to analyze polymorphisms within the lipocalin-2 gene and to determine their impact on antioxidant activity of milk from Holstein-Friesian cows. The genotyping was carried out by sequencing of PCR products. To determine the antioxidant activity of milk, the Trolox equivalent antioxidant capacity (TEAC) method was used. A total of four polymorphic sites were identified in the examined segment of the bovine lipocalin-2 gene. It was shown that cows of the CC genotype at the locus g.98793763G>C produced milk of significantly higher antioxidant capacity. The antioxidant capacity of milk also varied according to the age of cows, their daily milk yield, and SCC in milk.

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Effect of flavonoids content on antioxidant activity of commercial cosmetic plant extracts

Herba Polonica ◽

10.2478/hepo-2013-0017 ◽

2013 ◽

Vol 59 (3) ◽

pp. 63-75 ◽

Cited By ~ 3

Author(s):

Paulina Malinowska

Keyword(s):

Antioxidant Activity ◽

Antioxidant Capacity ◽

Plant Extracts ◽

Natural Antioxidants ◽

Trolox Equivalent Antioxidant Capacity ◽

Antioxidant Power ◽

Trolox Equivalent ◽

Ferric Reducing Antioxidant Power ◽

The Relationship ◽

Herb Extracts

Abstract The aim of this work was to evaluate the antioxidant capacity and flavonoids content in 10 commercial cosmetic plant extracts used in cosmetics industry. Antioxidant activity of plant extracts were measured using two methods: FRAP (Ferric Reducing Antioxidant Power) and TEAC (Trolox Equivalent Antioxidant Capacity). The relationship between flavonoids content and antioxidant capacity of plant extracts were checked. As a result of this research it was found that FRAP and TEAC values of plant extracts significantly depend on the flavonoids content. The highest antioxidant activity, both in FRAP and TEAC assays, was observed for arnica flowers, hawthorn flowers and lungwort herb extracts. These extracts can be used as source of natural antioxidants for the prolongation of the oxidative stability of cosmetic products. Additionally, they can replace synthetic antioxidants

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Electrochemical Determination of Antioxidant Capacity of Traditional Homemade Fruit Vinegars Produced with Double Spontaneous Fermentation

Microorganisms ◽

10.3390/microorganisms9091946 ◽

2021 ◽

Vol 9 (9) ◽

pp. 1946

Author(s):

Maja Chochevska ◽

Elizabeta Jančovska Seniceva ◽

Sanja Kostadinović Veličkovska ◽

Galaba Naumova-Leţia ◽

Valentin Mirčeski ◽

...

Keyword(s):

Antioxidant Activity ◽

Antioxidant Capacity ◽

Physicochemical Characterization ◽

Acetic Acid Bacteria ◽

Electrochemical Determination ◽

Raw Material ◽

Trolox Equivalent Antioxidant Capacity ◽

Total Phenolic ◽

Spontaneous Fermentation ◽

Trolox Equivalent

In the current study, the antioxidant activity of traditional homemade fruit vinegars (HMV) was estimated by measuring the rate of homogeneous redox reaction with 2,2′-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid radical cation (ABTS•+) using cyclic voltammetry. The antioxidant capacity of six HMV produced using traditional methods and the physicochemical characterization were measured in different vinegar production steps throughout a double spontaneous fermentation process, i.e., without any addition of yeasts or acetic acid bacteria. Their antioxidant capacity was compared with seven fruit commercial vinegars (ComV). Furthermore, the antioxidant capacity was independently measured with the TEAC (Trolox equivalent antioxidant capacity) assay, aiming at correlating with the electrochemical experimental data. Obtained results from both methods, the electrochemical and TEAC assays, interestingly indicated that all HMV have at least 10 times higher antioxidant activity than ComV. Furthermore, the large range of values for antioxidant capacity in samples of commercial vinegars from apples attested the importance of the raw material quality and technological procedures. The positive correlation between total phenolic content and antioxidant capacity measured by the two type of assays indicated that rose hip homemade vinegar (HMV5) has the highest antioxidant capacity. In contrast, the lowest levels of phenolic compounds and antioxidant capacity were found in apple and persimmon homemade vinegars (HMV1 and HMV6, respectively) which indicated that the type of fruit is crucial towards the production of high-quality vinegars. In this way, the use of traditional processes for the production of fruit vinegars proved to be very promising in terms of producing differentiated vinegars and, concomitantly, reaching high levels of health-promoting antioxidant capacities.

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Salmon (Salmo salar) Side Streams as a Bioresource to Obtain Potential Antioxidant Peptides after Applying Pressurized Liquid Extraction (PLE)

Marine Drugs ◽

10.3390/md19060323 ◽

2021 ◽

Vol 19 (6) ◽

pp. 323

Author(s):

Beatriz de la Fuente ◽

Noelia Pallarés ◽

Houda Berrada ◽

Francisco J. Barba

Keyword(s):

Antioxidant Activity ◽

Antioxidant Capacity ◽

Liquid Extraction ◽

Amino Acid Sequences ◽

Pressurized Liquid Extraction ◽

Trolox Equivalent Antioxidant Capacity ◽

Human Consumption ◽

Antioxidant Peptides ◽

Farmed Salmon ◽

Trolox Equivalent

The pressurized liquid extraction (PLE) technique was used to obtain protein extracts with antioxidant capacity from salmon muscle remains, heads, viscera, skin, and tailfins. A protein recovery percentage ≈28% was obtained for all samples except for viscera, which was ≈92%. These values represented an increase of 1.5–4.8-fold compared to stirring extraction (control). Different SDS-PAGE profiles in control and PLE extracts revealed that extraction conditions affected the protein molecular weight distribution of the obtained extracts. Both TEAC (Trolox equivalent antioxidant capacity) and ORAC (oxygen radical antioxidant capacity) assays showed an outstanding antioxidant activity for viscera PLE extract. Through liquid chromatography coupled with electrospray ionization triple time-of-flight (nanoESI qQTOF) mass spectrometry, 137 and 67 peptides were identified in control and PLE extracts from salmon viscera, respectively None of these peptides was found among the antioxidant peptides inputted in the BIOPEP-UMP database. However, bioinformatics analysis showed several antioxidant small peptides encrypted in amino acid sequences of viscera extracts, especially GPP (glycine-proline-proline) and GAA (glycine-alanine-alanine) for PLE extracts. Further research on the relationship between antioxidant activity and specific peptides from salmon viscera PLE extracts is required. In addition, the salmon side streams studied presented non-toxic levels of As, Hg, Cd, and Pb, as well as the absence of mycotoxins or related metabolites. Overall, these results confirm the feasible use of farmed salmon processing side streams as alternative sources of protein and bioactive compounds for human consumption.

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Chemical Composition and Antioxidant Activity of Essential Oils from Eugenia patrisii Vahl, E. punicifolia (Kunth) DC., and Myrcia tomentosa (Aubl.) DC., Leaf of Family Myrtaceae

Molecules ◽

10.3390/molecules26113292 ◽

2021 ◽

Vol 26 (11) ◽

pp. 3292

Author(s):

Celeste de Jesus Pereira Franco ◽

Oberdan Oliveira Ferreira ◽

Ângelo Antônio Barbosa de Moraes ◽

Everton Luiz Pompeu Varela ◽

Lidiane Diniz do Nascimento ◽

...

Keyword(s):

Mass Spectrometry ◽

Antioxidant Activity ◽

Chemical Composition ◽

Essential Oils ◽

Antioxidant Capacity ◽

Trolox Equivalent Antioxidant Capacity ◽

Germacrene D ◽

Trolox Equivalent ◽

Collection Period ◽

High Inhibition

Essential oils (EOs) were extracted from Eugenia patrisii, E. punicifolia, and Myrcia tomentosa, specimens A and B, using hydrodistillation. Gas chromatography coupled with mass spectrometry (GC/MS) was used to identify the volatile constituents present, and the antioxidant capacity of EOs was determined using diphenylpicryl-hydrazyl (DPPH) and trolox equivalent antioxidant capacity (TEAC) assays. For E. patrisii, germacrene D (20.03%), bicyclogermacrene (11.82%), and (E)-caryophyllene (11.04%) were identified as the major constituents of the EOs extracted from specimen A, whereas specimen B primarily comprised γ-elemene (25.89%), germacrene B (8.11%), and (E)-caryophyllene (10.76%). The EOs of E. punicifolia specimen A contained β-Elemene (25.12%), (E)-caryophyllene (13.11%), and bicyclogermacrene (9.88%), while specimen B was composed of (E)-caryophyllene (11.47%), bicyclogermacrene (5.86%), β-pinene (5.86%), and γ-muurolene (5.55%). The specimen A of M. tomentosa was characterized by γ-elemene (12.52%), germacrene D (11.45%), and (E)-caryophyllene (10.22%), while specimen B contained spathulenol (40.70%), α-zingiberene (9.58%), and γ-elemene (6.89%). Additionally, the chemical composition of the EOs was qualitatively and quantitatively affected by the collection period. Furthermore, the EOs of the studied specimens, especially specimen A of E. punicifolia, showed a greater antioxidant activity in DPPH rather than TEAC, as represented by a significantly high inhibition percentage (408.0%).

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Evaluation of total phenolic content, antioxidant activity, germination power, and yield of pigeon pea (Cajanus cajan) sprouts elicited using various Naalginate levels with different elicitation duration

Food Research ◽

10.26656/fr.2017.5(s2).005 ◽

2021 ◽

Vol 5 (S2) ◽

pp. 91-97

Author(s):

S. Ariviani ◽

K.C. Hapsari ◽

G. Fauza ◽

D. Ishartani ◽

W. Atmaka ◽

...

Keyword(s):

Antioxidant Activity ◽

Antioxidant Capacity ◽

Total Phenolic Content ◽

Cajanus Cajan ◽

Phenolic Content ◽

Pigeon Pea ◽

Trolox Equivalent Antioxidant Capacity ◽

Total Phenolic ◽

Antioxidant Power ◽

Trolox Equivalent

This study was aimed to investigate the total phenolic content and antioxidant activity of elicited pigeon pea (Cajanus cajan) sprouts prepared using various Na-alginate concentrations at different elicitation durations. The germination power and yield of the sprouts were also examined. Elicitation proved capable to improve the antioxidant capacity of legumes sprouts but the yield and germination rates were decreased due to the elicitation stress. In this study, elicitation conducted by immersing pigeon pea seeds in Na -alginate solutions (250, 300, and 350 ppm) for various duration (16, 20, and 24 hrs). The seeds were then germinated for 48 hrs to produce Na-alginate elicited pigeon pea sprouts. The results showed that the total phenolic content (TPC) and antioxidant activity (Trolox equivalent antioxidant capacity, TEAC, and Ferric reducing antioxidant power, FRAP) of elicited pigeon pea sprouts were significantly increased along with the increasing elicitation duration in all Na-alginate levels. The higher Na-alginate levels produce a higher level of TPC, TEAC, and FRAP values. On the other hand, germination power and the yield of the sprouts were significantly decreased along with increasing Na-alginate levels and elicitation duration. Elicitation using 350 ppm Na-alginate with an elicitation duration of 24 hrs produces elicited pigeon pea sprouts with the highest TPC and antioxidant activity, but lowest germination power and yield. These results have an important consequence in developing an elicitation technique to improve the antioxidant capacity of leguminous.

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Evaluation of the Antioxidant and Antimicrobial Properties of in vitro Cultured Drosera intermedia Extracts

Natural Product Communications ◽

10.1177/1934578x0900400809 ◽

2009 ◽

Vol 4 (8) ◽

pp. 1934578X0900400 ◽

Cited By ~ 1

Author(s):

Tomás Grevenstuk ◽

Sandra Gonçalves ◽

Sara Almeida ◽

Natacha Coelho ◽

Célia Quintas ◽

...

Keyword(s):

Antioxidant Activity ◽

Antioxidant Capacity ◽

Antimicrobial Properties ◽

Hexane Extract ◽

Trolox Equivalent Antioxidant Capacity ◽

Trolox Equivalent ◽

Hexane Extracts ◽

Diffusion Assay

Evaluation of the antioxidant activity of the methanol, water and n-hexane extracts of Drosera intermedia, determined by the Folin-Ciocalteau (F-C), trolox equivalent antioxidant capacity (TEAC) and oxygen radical antioxidant capacity (ORAC) assays showed that the methanol extract had the highest antioxidant activity (F-C: 378.6 ± 31.5 μmolGAE/mgextract; TEAC: 332.2 ± 29.1 μmolTE/mgextract; ORAC: 64.7 ± 7.8 μmolTE/mgextract). Antimicrobial activity was tested against seven bacterial and eight yeast strains using the agar diffusion assay, followed by the determination of minimum inhibitory concentrations (MIC). All tested D. intermedia extracts demonstrated strong antimicrobial properties with a broad spectrum of activity. However, the n-hexane extract exhibited much greater activity than water and methanol extracts. The most susceptible microorganisms to the n-hexane extract were Staphylococcus epidermidis ATCC 12228 and Candida albicans YP0175, for which a MIC value of 13.0 μg/mL was scored.

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