scholarly journals Role of pH in the recovery of bovine milk oligosaccharides from colostrum whey permeate by nanofiltration

2017 ◽  
Vol 66 ◽  
pp. 68-75 ◽  
Author(s):  
Joshua L. Cohen ◽  
Daniela Barile ◽  
Yan Liu ◽  
Juliana M.L.N. de Moura Bell
Keyword(s):  
Bovine Milk ◽  
Milk Oligosaccharides ◽  
Whey Permeate

scholarly journals An integrated bioprocess to recover bovine milk oligosaccharides from colostrum whey permeate

2018 ◽  
Vol 216 ◽  
pp. 27-35 ◽  
Author(s):  
Juliana M.L.N. de Moura Bell ◽  
Joshua L. Cohen ◽  
Leticia F.M.C. de Aquino ◽  
Hyeyoung Lee ◽  
Vitor L. de Melo Silva ◽  
...  
Keyword(s):  
Bovine Milk ◽  
Milk Oligosaccharides ◽  
Whey Permeate ◽  
Integrated Bioprocess

PSVII-9 Influence of lactose and milk oligosaccharides in whey permeate on jejunal mucosa-associated microbiota in nursery pigs during 7 to 11 kg BW

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 407-407
Author(s):  
Ki Beom Jang ◽  
Sung Woo Kim
Keyword(s):  
Alpha Diversity ◽  
Jejunal Mucosa ◽  
16S Rdna Sequencing ◽  
Sequencing Data ◽  
Milk Oligosaccharides ◽  
Whey Permeate ◽  
Nursery Pigs ◽  
Rdna Sequencing ◽  
Acid Producing Bacteria ◽  
Diversity Estimates

Abstract This study aimed to evaluate supplemental effects of milk carbohydrates in whey permeate on jejunal mucosa-associated microbiota in nursery pigs during 7 to 11 kg BW. A total of 720 pigs at 7.5 kg BW were allotted to 6 treatments (6 pens/treatment and 20 pigs/pen). Treatments were 6 levels of whey permeate supplementation (0, 3.75, 7.50, 11.25, 15.00, and 18.75%) and fed to pigs for 11 d. On d 11, 36 pigs representing median BW of each pen were euthanized to collect the jejunal mucosa to evaluate microbiota in the jejunum by 16S rDNA sequencing. Data were analyzed using contrasts in MIXED procedure of SAS. Whey permeate contained 76.3% lactose and 0.4% milk oligosaccharides. Increasing whey permeate supplementation from 0 to 18.75% did not affect the alpha-diversity estimates of microbiota. Whey permeate supplementation tended to decrease (P = 0.073, 1.59 to 1.22) Firmicutes:Bacteroidetes compared with no addition of whey permeate. Increasing whey permeate supplementation tended to linearly increase Bifidobacteriaceae (P = 0.089, 0.73 to 1.11), decrease Enterobacteriaceae (P = 0.091, 1.04 to 0.52), decrease Stretococcaceae (P = 0.094, 1.50 to 0.71), and caused quadratic changes (P < 0.05) on Lactobacillaceae (maximum: 9.14% at 12.91% whey permeate). Increasing whey permeate supplementation caused a quadratic change (P < 0.05) on Lactobacillus_Salivarius (maximum: 0.92% at 7.35% whey permeate) and tended to cause quadratic changes on Lactobacillus_Rogosae (P = 0.083; maximum: 0.53% at 8.45% whey permeate) and Lactobacillus_Mucosae (P = 0.092; maximum: 0.70% at 6.98% whey permeate). In conclusion, supplementation of whey permeate as sources of lactose and milk oligosaccharides at a range from 7 to 13% seems to be beneficial to nursery pigs by increasing the abundance of lactic acid-producing bacteria in the jejunal mucosa.

scholarly journals Bovine Milk Oligosaccharides with Sialyllactose for Preterm Piglets

Nutrients ◽  
2018 ◽  
Vol 10 (10) ◽  
pp. 1489 ◽  
Author(s):  
Karina Obelitz-Ryom ◽  
Amalie Rendboe ◽  
Duc Nguyen ◽  
Silvia Rudloff ◽  
Anne Brandt ◽  
...  
Keyword(s):  
Bacterial Colonization ◽  
Bovine Milk ◽  
Protective Effects ◽  
Gut Development ◽  
Artificial Rearing ◽  
Milk Oligosaccharides ◽  
Term Infants ◽  
Brain Functions ◽  
Near Term ◽  
Dietary Treatments

Oligosaccharides support gut development and bacterial colonization in term infants, but it is unknown if they benefit preterm infants. Using preterm pigs, we investigated effects of bovine milk supplements enriched with oligosaccharides to improve gut development and colonization. Caesarean-delivered preterm pigs (n = 57) were reared for 19 days. The pigs were fed bovine milk supplemented with an oligosaccharide-enriched whey containing sialyllactose, or a heterogeneous oligosaccharide ingredient. To evaluate the influence of artificial rearing, near-term, vaginally born pigs raised by their sow (n = 12) were compared with artificially reared, caesarean-delivered near-term pigs (n = 14). In preterm pigs, the clinical outcome, gut function, gut microbiota, and systemic immunity were similar among dietary treatments. Natural rearing increased growth rates, gut functions, colon short chain fatty acid concentrations and bacterial diversity, relative to artificial rearing. In conclusion, supplements with bovine milk oligosaccharides were well tolerated, but did not improve gut maturation or clinical outcomes in artificially reared preterm piglets. Immaturity at birth, coupled with artificial rearing, may render the neonate unresponsive to the gut-protective effects of milk oligosaccharides. Whether bovine milk oligosaccharides may affect other endpoints (e.g., brain functions) in conditions of immaturity remains to be investigated.

scholarly journals Tolerability and safety of the intake of bovine milk oligosaccharides extracted from cheese whey in healthy human adults

2017 ◽  
Vol 6 ◽  
Author(s):  
Jennifer T. Smilowitz ◽  
Danielle G. Lemay ◽  
Karen M. Kalanetra ◽  
Elizabeth L. Chin ◽  
Angela M. Zivkovic ◽  
...  
Keyword(s):  
Bovine Milk ◽  
Evolutionary Relationship ◽  
Microbial Metabolism ◽  
Stool Consistency ◽  
Placebo Control ◽  
Resonance Spectroscopy ◽  
Faecal Microbiota ◽  
Milk Oligosaccharides ◽  
Healthy Human ◽  
Bacterial Populations

AbstractMechanistic research suggests a unique evolutionary relationship between complex milk oligosaccharides and cognate bifidobacteria enriched in breast-fed infants. Bovine milk oligosaccharides (BMO) were recently identified as structurally and functionally similar to human milk oligosaccharides. The present single-blind three-way crossover study is the first to determine the safety and tolerability of BMO consumption by healthy human participants (n 12) and its effects on faecal microbiota and microbial metabolism. Participants consumed each supplement (placebo-control; low- and high-BMO doses) for eleven consecutive days, followed by a 2-week washout period prior to initiating the next supplement arm. Low and high BMO doses were consumed as 25 and 35 % of each individual's daily fibre intake, respectively. Safety and tolerability were measured using standardised questionnaires on gut and stomach discomfort and stool consistency. Faecal extracts were profiled for bacterial populations by next-generation sequencing (NGS) and bifidobacteria presence was confirmed using quantitative PCR. Urine was analysed for changes in microbial metabolism using nuclear magnetic resonance spectroscopy (1H-NMR). Consumption of both the low and high BMO doses was well tolerated and did not change stool consistency from baseline. Multivariate analysis of the NGS results demonstrated no change in faecal microbiota phyla among the placebo-control and BMO supplement groups. In conclusion, BMO supplementation was well tolerated in healthy adults and has the potential to shift faecal microbiota toward beneficial strains as part of a synbiotic treatment with probiotic cultures that selectively metabolise oligosaccharides.

Characterization of proteins with Siaα2-3/6Gal-linked glycans from bovine milk and role of their glycans against influenza A virus

2018 ◽  
Vol 9 (10) ◽  
pp. 5198-5208 ◽  
Author(s):  
Hanjie Yu ◽  
Yaogang Zhong ◽  
Zhiwei Zhang ◽  
Xiawei Liu ◽  
Kun Zhang ◽  
...  
Keyword(s):  
Influenza A Virus ◽  
Influenza A ◽  
Bovine Milk ◽  
Milk Proteins ◽  
Biological Functions ◽  
Wide Range ◽  
Range Of Functions ◽  
Bovine Milk Proteins

The bovine milk proteins have a wide range of functions, but the role of the attached glycans in their biological functions has not been fully understood yet.

scholarly journals Profiling of aminoxyTMT-labeled bovine milk oligosaccharides reveals substantial variation in oligosaccharide abundance between dairy cattle breeds

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Randall C. Robinson ◽  
Nina A. Poulsen ◽  
Emeline Colet ◽  
Chloe Duchene ◽  
Lotte Bach Larsen ◽  
...  
Keyword(s):  
Dairy Cattle ◽  
Bovine Milk ◽  
Substantial Variation ◽  
Milk Oligosaccharides ◽  
Cattle Breeds

scholarly journals Role of Milk-Derived Opioid Peptides and Proline Dipeptidyl Peptidase-4 in Autism Spectrum Disorders

Nutrients ◽  
2019 ◽  
Vol 11 (1) ◽  
pp. 87 ◽  
Author(s):  
Beata Jarmołowska ◽  
Marta Bukało ◽  
Ewa Fiedorowicz ◽  
Anna Cieślińska ◽  
Natalia Karolina Kordulewska ◽  
...  
Keyword(s):  
Opioid Peptides ◽  
Mononuclear Cells ◽  
Bovine Milk ◽  
Dipeptidyl Peptidase ◽  
Autism Spectrum ◽  
Control Group ◽  
Healthy Children ◽  
Dipeptidyl Peptidase 4 ◽  
Significant Difference

Opioid peptides released during digestion of dietary proteins such as casein, were suggested to contribute to autism development, leading to the announcement of opioid excess hypothesis of autism. This paper examines role of enzyme proline dipeptidyl peptidase-4 (DPPIV; EC 3.4.14.5) and it is exogenous substrate, β-casomorphin-7 (BCM7) in autism etiology. Our study included measurements of DPPIV and BCM7 concentrations in serum and urine, which were analyzed with ELISA assays and activity of DPPIV was measured by colorimetric test. The effect of opioid peptides from hydrolysed bovine milk on DPPIV gene expression in peripheral blood mononuclear cells (PBMC) in autistic and healthy children was determined using the Real-Time PCR (Polymerase Chain Reaction) method. Our research included 51 healthy children and 86 children diagnosed with autism spectrum disorder (ASD, ICDF84). We determined that the concentration of BCM7 in serum was significantly, 1.6-fold, higher in the ASD group than in controls (p < 0.0001). Concentration of DPPIV was found to also be significantly higher in serum from ASD children compared to the control group (p < 0.01), while we did not notice significant difference in enzymatic activity of serum DPPIV between the two study groups. We confirmed correlation according to the gender between analyzed parameters. The inspiration for this study emanated from clinical experience of the daily diet role in relieving the symptoms of autism. Despite this, we have concluded that milk-derived opioid peptides and DPPIV are potentially factors in determining the pathogenesis of autism; conducted studies are still limited and require further research.

scholarly journals The Role of Human Milk Oligosaccharides and Probiotics on the Neonatal Microbiome and Risk of Necrotizing Enterocolitis: A Narrative Review

Nutrients ◽  
2020 ◽  
Vol 12 (10) ◽  
pp. 3052
Author(s):  
Lila S. Nolan ◽  
Jamie M. Rimer ◽  
Misty Good
Keyword(s):  
Human Milk ◽  
Preterm Infants ◽  
Necrotizing Enterocolitis ◽  
Mode Of Delivery ◽  
Intestinal Barrier ◽  
Vulnerable Population ◽  
Human Milk Oligosaccharides ◽  
Milk Oligosaccharides ◽  
Intestinal Homeostasis

Preterm infants are a vulnerable population at risk of intestinal dysbiosis. The newborn microbiome is dominated by Bifidobacterium species, though abnormal microbial colonization can occur by exogenous factors such as mode of delivery, formula feeding, and exposure to antibiotics. Therefore, preterm infants are predisposed to sepsis and necrotizing enterocolitis (NEC), a fatal gastrointestinal disorder, due to an impaired intestinal barrier, immature immunity, and a dysbiotic gut microbiome. Properties of human milk serve as protection in the prevention of NEC. Human milk oligosaccharides (HMOs) and the microbiome of breast milk are immunomodulatory components that provide intestinal homeostasis through regulation of the microbiome and protection of the intestinal barrier. Enteral probiotic supplements have been trialed to evaluate their impact on establishing intestinal homeostasis. Here, we review the protective role of HMOs, probiotics, and synbiotic combinations in protecting a vulnerable population from the pathogenic features associated with necrotizing enterocolitis.

scholarly journals The Role of Two Human Milk Oligosaccharides, 2′-Fucosyllactose and Lacto-N-Neotetraose, in Infant Nutrition

2019 ◽  
Vol 22 (4) ◽  
pp. 330 ◽  
Author(s):  
Badriul Hegar ◽  
Yulianti Wibowo ◽  
Ray Wagiu Basrowi ◽  
Reza Gunadi Ranuh ◽  
Subianto Marto Sudarmo ◽  
...  
Keyword(s):  
Human Milk ◽  
Infant Nutrition ◽  
Human Milk Oligosaccharides ◽  
Milk Oligosaccharides
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