Antioxidant activity of exopolysaccharide from probiotic strain Enterococcus faecium (BDU7) from Ngari

Abstract The effect of Enterococcus faecium CCM7420 (EF) - enterocin-producing and probiotic strain of rabbit origin, Eleutherococcus senticosus extract (ES) and their combination (ES+EF) was determined on selected bacteria in faeces and caecum content, leukocytes phagocytosis, blood biochemistry and growth performance. Ninety-six weaned rabbits were divided into 3 experimental (ES, EF, ES+EF) and control group (CG). The rabbits in the groups ES and EF+ES were fed commercial diet enriched with E. senticosus extract (30 g/100 kg feed), rabbits in groups EF and CG were fed untreated diet. The rabbits in the EF and ES+EF groups were administered with an overnight culture of E. faecium CCM7420 strain (500 μl/animal/day into water, 109 CFU/ml). The treatment period lasted 21 days. The microbiological examinations in faecal samples confirmed the presence of E. faecium CCM7420 strain. In groups EF and ES+EF, the reduction of faecal coliforms, Pseudomonas-like sp., Clostridium-like sp. and S. aureus was recorded. Leucocyte phagocytosis significantly increased in all experimental groups (P<0.0001) compared to CG. The lowest GPx values were measured in the ES+EF group. Higher total protein, triglycerides and calcium concentrations were detected in experimental groups compared to CG. The cholesterol concentration decreased in the ES group. The highest average daily gain was recorded in EF group; in ES+EF the better feed conversion ratio and no mortality was recorded. These results indicated that the dietary supplementation with the E. faecium CCM7420 and E. senticosus extract stimulate the leukocytes phagocytosis and reduces the potential pathogens in rabbits digestive tract without oxidative stress and improve the growth performance.

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Anti-adhesion of probiotic Enterococcus faecium WEFA23 against five pathogens and the beneficial effect of its S-layer proteins against Listeria monocytogenes

Canadian Journal of Microbiology ◽

10.1139/cjm-2018-0031 ◽

2019 ◽

Vol 65 (3) ◽

pp. 175-184

Author(s):

Yao He ◽

Xiongpeng Xu ◽

Fen Zhang ◽

Di Xu ◽

Zhengqi Liu ◽

...

Keyword(s):

Listeria Monocytogenes ◽

Enterococcus Faecium ◽

Probiotic Strain ◽

Antagonistic Activity ◽

Bacterial Surface ◽

Colony Forming Units ◽

Infant Feces ◽

Adhesion Activity ◽

Displacement Assays ◽

Different Levels

Enterococcus faecium WEFA23 is a potential probiotic strain isolated from Chinese infant feces. In this study, the antagonistic activity of E. faecium WEFA23 on adhesion to pathogens was investigated. Enterococcus faecium WEFA23 was able to compete, exclude, and displace the adhesion of Escherichia coli O157:H7, Salmonella Typhimurium ATCC 13311, Listeria monocytogenes CMCC54007, Staphylococcus aureus CMCC26003, and Shigella sonnei ATCC 25931 to Caco-2 cells. Among them, L. monocytogenes achieved the strongest inhibition rate in both competition and displacement assays. Those anti-adhesion capacities were related to the bacterial physicochemical properties (hydrophobicity, auto-aggregation, and co-aggregation) of the bacterial surface. For L. monocytogenes, the anti-adhesion capacity was affected by the heat treatment, cell density, and growth phase of E. faecium WEFA23; 108 colony-forming units of viable cells per millilitre at the stationary phase exhibited the strongest anti-adhesion activity. In addition, removal of S-layer proteins of E. faecium WEFA23 by treatment with 5 mol/L LiCl significantly decreased its adhesion capacity, and those S-layer proteins were able to compete, displace, and exclude L. monocytogenes at different levels. Both cells and S-layer proteins of E. faecium WEFA23 significantly reduced the apoptosis of Caco-2 cells induced by L. monocytogenes, which was mediated by caspase-3 activation. This study might be helpful in understanding the anti-adhesion mechanism of probiotics against pathogens.

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Individual responses of mother sows to a probiotic Enterococcus faecium strain lead to different microbiota composition in their offspring

Beneficial Microbes ◽

10.3920/bm2013.0021 ◽

2013 ◽

Vol 4 (4) ◽

pp. 345-356 ◽

Cited By ~ 36

Author(s):

I.C. Starke ◽

R. Pieper ◽

K. Neumann ◽

J. Zentek ◽

W. Vahjen

Keyword(s):

Enterococcus Faecium ◽

Denaturing Gradient Gel Electrophoresis ◽

Probiotic Strain ◽

Quantitative Composition ◽

Pronounced Increase ◽

Gradient Gel Electrophoresis ◽

Suckling Piglets ◽

Weaning Piglets ◽

The Impact ◽

Faecium Strain

Pregnant gilts were fed the probiotic Enterococcus faecium NCIMB10415 (SF68) one month before birth of piglets. DNA extracts of sow faeces taken in weekly intervals as well as extracts from the intestine of their offspring during the suckling period at 12 and 26 days of life were analysed by denaturing gradient gel electrophoresis (DGGE) and quantitative PCR. DGGE profiles of faecal bacterial communities from three out of six probiotic-fed sows were distinctly different from the control and other probiotic-fed sows at all time points after probiotic supplementation. The probiotic-fed sows and their offspring were therefore divided into non-responder (n=3) and responder (n=3) groups. The probiotic strain significantly increased faecal lactobacilli cell numbers in mother sows, which could be assigned to a significant increase of Lactobacillus amylovorus and Lactobacillus acidophilus. Responding sows showed a more pronounced increase than non-responding sows. Similarly, suckling piglets from non-responding and responding sows showed numeric and significant differences for different bacterial groups and species. DGGE profiles of suckling piglets from responding sows also grouped more closely than profiles from control animals. Non-metric multiscaling of suckling piglets showed the same tendency for suckling piglets, but not for post-weaning piglets. This study showed that the probiotic E. faecium strain modified the faecal microbiota of sows. This modification is carried over to their offspring, but leads to changes that do not mirror the quantitative composition in the mother sow. Individual variations in the bacterial composition of mother sows before probiotic feed intake may influence the impact of a probiotic in sows and their offspring.

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Pilot experiment in chickens challenged with Campylobacter jejuni CCM6191 administered enterocin M-producing probiotic strain Enterococcus faecium CCM8558 to check its protective effect

Czech Journal of Animal Science ◽

10.17221/12/2017-cjas ◽

2017 ◽

Vol 62 (No. 11) ◽

pp. 491-500 ◽

Cited By ~ 3

Author(s):

A. Laukova ◽

M. Pogany Simonova ◽

I. Kubasova ◽

S. Gancarcikova ◽

I. Placha ◽

...

Keyword(s):

Campylobacter Jejuni ◽

Post Infection ◽

Enterococcus Faecium ◽

Probiotic Strain ◽

Ringer Solution ◽

Culture Collection ◽

Free Access ◽

Food Borne ◽

Enterocin M ◽

Campylobacter Spp

Campylobacter jejuni is one of the most common food-borne pathogens and chickens are the main source of these bacteria. Enterococcus faecium AL41, enterocin M-producing strain (deponed to the Czech Culture Collection of Microorganisms, Brno, Czech Republic – CCM8558) is our isolate previously applied e.g. in broiler rabbits with beneficial effect. In this study it was used in a 11-day experiment with chickens (1-day-old, breed Cobb 500, n = 40) challenged with C. jejuni. Birds had free access to feed and water; they were randomly divided into four groups per 10 chicks each: control (CG), E. faecium CCM8558 (EG1), CCM8558 + C. jejuni CCM6191 (EG2), CCM6191 (EG3). E. faecium CCM8558 (109 CFU/ml, 200 µl) in Ringer solution was administered daily per os to EG1 and EG2 for 7 days (from day 0–1). EG3 and EG2 were infected individually per os (day 4, CCM6191, 108 CFU/ml in Ringer solution, 200 µl). For microbiota evaluation, faecal mixtures (n = 5) were sampled on day 0–1 (1st sampling), on day 7 (2nd sampling), and on day 11 (3rd sampling) (day 7 of CCM8558 application, day 4 post-infection; day 4 of CCM8558 cessation, day 7 post-infection). Five birds from each group were sacrificed. CCM8558 sufficiently colonized chickens. In faeces of EG2 a tendency to reduce Campylobacter spp. (day 7 of application, day 4 of infection) was noted compared to EG2 (day 11, day 4 of cessation, day 7 post-infection; difference 1.21 log cycles), while in EG3 CCM6191 strain was not reduced. Phagocytic activity (PA) values were significantly higher in infected groups compared to CG and EG1. A significant increase in PA was also noted in EG2 and EG3 at the end of experiment compared to CG or EG1. The strain additive did not evoke oxidative stress. Biochemical parameters were influenced to the reference levels.

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Effects of the probiotic Enterococcus faecium NCIMB 10415 on selected lactic acid bacteria and enterobacteria in co-culture

Beneficial Microbes ◽

10.3920/bm2014.0052 ◽

2015 ◽

Vol 6 (3) ◽

pp. 345-352 ◽

Cited By ~ 9

Author(s):

I.C. Starke ◽

J. Zentek ◽

W. Vahjen

Keyword(s):

Intestinal Microbiota ◽

Enterococcus Faecium ◽

Lactobacillus Reuteri ◽

Specific Growth ◽

Probiotic Strain ◽

Lactobacillus Mucosae ◽

Vitro Effect ◽

Reference Strains ◽

Faecium Strain

Enterococcus faecium NCIMB 10415 is used as a probiotic for piglets and has been shown to modify the porcine intestinal microbiota. However, the mode of action of this probiotic modification is still unclear. One possible explanation is the direct growth inhibiting or stimulating effect of the probiotic on other indigenous bacteria. Therefore, the aim of the present study was to examine the growth interactions of the probiotic with different indigenous porcine bacteria in vitro. Reference strains were cultivated with the probiotic E. faecium strain NCIMB10415 (SF68) in a checkerboard assay with 102 to 105 cells/ml inoculum per strain. Growth kinetics were recorded for 8 h and used to determine specific growth of the co-cultures. Additionally, total DNA was extracted from the co-cultures at the end of the incubation to verify which strain in the co-culture was affected. Co-cultivation with eight Enterococcus spp. tester strains showed strain-specific growth differences. Three of four E. faecium strains were not influenced by the probiotic strain. PCR results showed reduced growth of the probiotic strain in co-culture with E. faecium DSM 6177. Three of four Enterococcus faecalis strains showed reduced specific growth in co-culture with the probiotic strain. However, E. faecalis DSM 20478 impaired growth of the probiotic E. faecium strain. The growth of Lactobacillus johnsonii DSM 10533 and Lactobacillus reuteri DSM 20016 was enhanced in co-culture with the probiotic strain, but co-cultivations with Lactobacillus mucosae DSM13345 or Lactobacillus amylovorus DSM10533 showed no differences. Co-cultures with the probiotic E. faecium showed no impact on the growth rate of four different enterobacterial reference strains (2 strains of Salmonella enterica and 2 strains of Escherichia coli), but PCR results showed reduced cell numbers for a pathogenic E. coli isolate at higher concentration of the probiotic strain. As the in vitro effect of the probiotic E. faecium on enterococci was strain specific and the growth of certain Lactobacillus spp. was enhanced by the probiotic, these results indicate a direct effect of the probiotic on certain members of the porcine gastro intestinal microbiota.

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Beneficial effects of Enterococcus faecium EF9a administration in rabbit diet

World Rabbit Science ◽

10.4995/wrs.2020.11189 ◽

2020 ◽

Vol 28 (4) ◽

pp. 169

Author(s):

Monika Pogány Simonová ◽

Andrea Lauková ◽

Ľubica Chrastinová ◽

Iveta Plachá ◽

Renáta Szabóová ◽

...

Keyword(s):

Enterococcus Faecium ◽

Live Weight ◽

Probiotic Strain ◽

Good Health ◽

Health Condition ◽

Daily Weight Gain ◽

Commercial Diet ◽

Beneficial Effects ◽

Access To Water ◽

And Control

Forty-eight rabbits aged five weeks (Hycole breed, both sexes) were divided into experimental (EG) and control (CG) groups, 24 animals in each, and fed a commercial diet with access to water ad libitum. Rabbits in EG had Enterococcus faecium EF9a probiotic strain added to their drinking water (1.0×109 colony forming units/mL 500 μL/d/animal) for 28 d (between 35 and 63 d). The experiment lasted for 42 d. The animals remained in good health condition throughout the experiment, and no morbidity and mortality was noted. There was a higher live weight at 63 d of age (+34 g; P<0.0001), final live weight at 77 d of age (+158 g; P=0.0483), and average daily weight gain between 63 and 77 d of age in the EG group rabbits than in CG group rabbits (+8 g/d; P<0.0001). No significant changes in caecal lactic acid and total volatile fatty acid concentrations, jejunal morphological parameters and phagocytic activity were noted during the treatment. The tested serum parameters were within the range of the reference values. EF9a strain sufficiently established itself in the rabbit’s gastrointestinal tract. At 63 d of age, a significant decrease in coliforms (P<0.05), coagulase-positive staphylococci (P<0.01), pseudomonads (P<0.01) and coagulasenegative staphylococci (CoNS, P<0.001) was noted in the faeces of the EG group rabbits compared to the CG rabbits. Antimicrobial effects of EF9a strain in the caecum against coliforms (P<0.001), CoNS (P=0.0002) and pseudomonads (P=0.0603) and in the appendix (coliforms, P<0.05) were detected.

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Development of live vaccine based on the Enterococcus faecium L3 vector for prevention influenza infections

10.21203/rs.3.rs-749575/v1 ◽

2021 ◽

Author(s):

Yulia Desheva ◽

Galina Leontieva ◽

Tatiana Kramskaya ◽

Tatiana Gupalova ◽

Olga Kopteva ◽

...

Keyword(s):

Influenza Virus ◽

Enterococcus Faecium ◽

Influenza Infection ◽

Probiotic Strain ◽

Recombinant Vaccines ◽

Local Immunity ◽

Epidemic Season ◽

The Third ◽

Genes Encoding ◽

Influenza Virus Neuraminidase

Abstract Probiotic microorganisms are currently considered as a promising platform for the development of recombinant vaccines expressing foreign antigens. In this study we generated and evaluated the live mucosal recombinant vaccine by integrating genes encoding influenza virus neuraminidase (NA) of N2 subtype into the DNA of the probiotic strain Enterococcus faecium L3 (L3). We confirmed NA expression in the pili of L3 using immune electron microscopy. Mice were fed with a probiotic vaccine containing the NA gene (L3-NA) or pure L3. Oral administration of L3-NA caused detectable increase of virus-specific serum IgG and local IgA after the third feeding. A the same time, single spleen cell suspensions were stimulated with whole A(H1N1)pdm09 virus followed by flow cytometry. In mice received L3-NA, the content of cytotoxic T-lymphocytes was more pronounced compared to mice receiving pure L3. Immunization with L3-NA increased the survival rate by 34% when the mice were infected using A(H1N1)pdm09 influenza virus after the third feeding. After S. pneumonia post-influenza infection, the L3-NA immunized mice were 50% more protected from lethality in comparison with L3 fed mice. Thus, a live probiotic vaccine candidate based on L3 induced the formation of systemic and local immunity and provide protection against complicated influenza. The approach based on a probiotic vaccine expressing viral epitopes can allow repeated immunization during epidemic season.

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Probiotic properties of Enterococcus faecium CE5-1 producing a bacteriocin-like substance and its antagonistic effect against antibiotic-resistant enterococci in vitro

Czech Journal of Animal Science ◽

10.17221/6386-cjas ◽

2012 ◽

Vol 57 (No. 11) ◽

pp. 529-539 ◽

Cited By ~ 10

Author(s):

K. Saelim ◽

N. Sohsomboon ◽

S. Kaewsuwan ◽

S. Maneerat

Keyword(s):

Enterococcus Faecium ◽

Probiotic Strain ◽

Antagonistic Effect ◽

Penicillin G ◽

Probiotic Properties ◽

Antibiotic Resistant ◽

Good Tolerance ◽

Probiotic Potential ◽

Enterocin A

A bacteriocin-like substance (BLS) producing Enterococcus faecium CE5-1 was isolated from the gastrointestinal tract (GIT) of Thai indigenous chickens. Investigations of its probiotic potential were carried out. The competition between the BLS probiotic strain and antibiotic-resistant enterococci was also studied. Ent. faecium CE5-1 exhibited a good tolerance to pH 3.0 after 2 h and in 7% fresh chicken bile after 6 h, but the viability of Ent. faecium CE5-1 decreased by about 2–3 log CFU/ml after 2 h incubation in pH 2.5. It was susceptible to the antibiotics tested (tetracycline, erythromycin, penicillin G, and vancomycin). The maximum BLS production from Ent. faecium CE5-1 was observed at 15 h of cultivation. It showed activity against Listeria monocytogenes DMST17303, Pediococcus pentosaceus 3CE27, Lactobacillus sakei subsp. sakei JCM1157, and antibiotic-resistant enterococci. The detection by polymerase chain reaction (PCR) in the enterocin structural gene determined the presence of enterocin A gene in Ent. faecium CE5-1 only. Ent. faecium CE5-1 showed the highest inhibitory activity against two antibiotic-resistant Ent. faecalis VanB (from 6.68 to 4.29 log CFU/ml) and Ent. gallinarum VanC (from 6.76 to 4.31 log CFU/ml) after 12 h of co-cultivation. The results show the future possible use of Ent. faecium CE5-1 as a probiotic strain for livestock to control antibiotic-resistant enterococci.  

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Estimation of the antioxidant properties of milk protein preparations hydrolyzed by Lactobacillus helveticus T80, T105 and B734

Czech Journal of Food Sciences ◽

10.17221/477/2017-cjfs ◽

2019 ◽

Vol 37 (No. 4) ◽

pp. 260-267 ◽

Cited By ~ 2

Author(s):

Katarzyna Skrzypczak ◽

Bartosz Sołowiej ◽

Adam Waśko ◽

Anna Kononiuk ◽

Waldemar Gustaw

Keyword(s):

Antioxidant Activity ◽

Milk Protein ◽

Antioxidant Properties ◽

Reducing Power ◽

Probiotic Strain ◽

Protein Isolate ◽

Lactobacillus Helveticus ◽

Protein Preparation ◽

Oxidation Processes ◽

Chelating Activity

The investigation determined the influence of Lactobacillus helveticus strains (T80, T105, B734 and DSMZ 20075) and Lactobacillus acidophilus La-05 (probiotic strain) on the antioxidant activity of whey and milk protein preparations. In order to indicate possible mechanism of antioxidant activity of the obtained hydrolysates, the reducing power (RP) assay, ability to neutralize free radicals and Fe2+ chelating activity analysis were employed. The results suggest that antioxidant activity of tested hydrolysates depends on the type of protein preparation (substrate) and the applied bacterial strain. The strongest antioxidant activity was exhibited by whey protein isolate hydrolysate obtained by using L. helveticus T80. A majority of fermented products obtained with the use of the probiotic strain were characterized by higher antioxidant properties than those obtained by application of L. helveticus. The solution of caseinoglicomacropeptide fermented by strain T105 exhibited the highest RP values, while α-lactalbumin hydrolysed by probiotic strain (La-5) was characterized by the strongest Fe2+ chelating activity. The analysed protein preparations and their hydrolysates obtained with using tested bacteria might potentially be applied in food products in order to inhibit oxidation processes.

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Production and characteristic quality of probiotic Labneh cheese supplemented with broccoli florets

British Food Journal ◽

10.1108/bfj-05-2021-0554 ◽

2021 ◽

Vol ahead-of-print (ahead-of-print) ◽

Author(s):

Hani S. Abd El-Montaleb ◽

Khaled Abd-Elhakam Abbas ◽

Mai Ali Mwaheb ◽

Shaimaa Mohamed Hamdy

Keyword(s):

Antioxidant Activity ◽

Lactobacillus Casei ◽

Health Benefits ◽

Streptococcus Thermophilus ◽

Probiotic Strain ◽

Soluble Nitrogen ◽

Content Type ◽

Lower Protein ◽

Different Levels

PurposeThe purpose of this study is to develop functional probiotic Labneh cheeses supplemented with broccoli florets.Design/methodology/approachProbiotic Labneh cheese was produced using broccoli florets paste at four different levels (0, 5, 10 and 15%), with Lactobacillus casei NRRL B-1922 as a probiotic strain, to evaluate its physicochemical, phenols, antioxidant activity, minerals, vitamins, textural, microbiological and sensory characteristics during storage for 15 days.FindingsThe results indicated that Labneh cheese with added broccoli paste exhibited significantly (p = 0.05) higher level of moisture, acidity, soluble nitrogen, phenols, antioxidant activity, minerals and B vitamins, and lower protein, fat, ash and pH values when compared to control Labneh cheese. Textural analysis of Labneh cheese indicated that Labneh with higher level of broccoli (15%) exhibited harder texture than others. Higher viable counts of Lactobacillus casei and Streptococcus thermophilus were detected in Labneh with broccoli paste, and the counts (107 cfu/g) were higher than the number should be present to achieve their health benefits. The most acceptable Labneh cheeses were those supplemented with 5 and 10% broccoli paste.Originality/valueThis study revealed broccoli florets could enhance the growth of Lactobacillus casei and Streptococcus thermophilus in the Labneh matrix, which resulted in a wider spectrum of health benefits of Labneh cheese to the consumers.

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