Antimicrobial resistance among Campylobacter jejuni isolated from raw poultry meat at retail level in Denmark

2006 ◽  
Vol 107 (3) ◽  
pp. 250-255 ◽  
Author(s):  
S ANDERSEN ◽  
P SAADBYE ◽  
N SHUKRI ◽  
H ROSENQUIST ◽  
N NIELSEN ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Campylobacter Jejuni ◽  
Poultry Meat ◽  
Raw Poultry

Metronidazole Resistance in Campylobacter jejuni from Poultry Meat

2006 ◽  
Vol 69 (4) ◽  
pp. 932-934 ◽  
Author(s):  
SIGRID RITA ANDERSEN ◽  
NASEER M. SHUKRI ◽  
JEPPE BOEL ◽  
PETER SAADBYE
Keyword(s):  
Campylobacter Jejuni ◽  
Significant Proportion ◽  
Bimodal Distribution ◽  
Poultry Meat ◽  
First Report ◽  
Turkey Meat ◽  
Metronidazole Resistance ◽  
Agar Dilution ◽  
Raw Poultry ◽  
Testing Range

The occurrence of metronidazole resistance was investigated among Campylobacter jejuni in raw poultry meat collected from supermarkets. MICs were determined by the agar dilution procedure in the testing range of 3 to 60 μg/ml metronidazole. The MICs showed a bimodal distribution with a significant proportion of metronidazole-resistant isolates among C. jejuni from raw broiler and turkey meat. Metronidazole resistance occurred most frequently among turkey meat isolates (P < 0.005). This is the first report of foodborne bacteria carrying metronidazole resistance.

scholarly journals Differences in antimicrobial resistance of Salmonella spp. isolated from humans, animals and food products in Kazakhstan

2021 ◽  
Vol 72 (3) ◽  
pp. 3091
Author(s):  
R RYCHSHANOVA ◽  
M RUZAUSKAS ◽  
G CHUZHEBAYEVA ◽  
R MOCKELIUNAS ◽  
N MAMIYEV ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Horizontal Gene Transfer ◽  
Animal Species ◽  
Food Products ◽  
Poultry Meat ◽  
Genetic Determinants ◽  
Salmonella Spp ◽  
Genes Encoding ◽  
Raw Poultry ◽  
Different Sources

The aims of this study were twofold: 1. to isolate Salmonella spp. from different sources in Kazakhstan, to determine their serovars and resistance profiles and 2. to evaluate similarities in antimicrobial susceptibility of Salmonella spp. isolated from humans, animals and food products. From the10 212 samples tested Salmonella spp. Were isolated in 47 cases. The predominant serovar isolated from humans and food products was S. Enteritidis. Although different animal species were tested the Salmonella spp. were isolated mainly from chickens and ducks. S. Enteritidis and S. Typhimurium were the most prevalent serovars in raw poultry meat. The most frequent resistances were those to nalidixic acid, ampicillin and tetracycline. Human isolates demonstrated lower resistance compared to animal and food isolates. The genes encoding antimicrobial resistance in human isolates in most cases were absent except for some isolates which harboured tet, sul, stre and bla TEM genes encoding resistance to the oldest antimicrobial classes. The situation in animals and food products was less beneficial as besides different genes, integrons associated with the horizontal gene transfer were detected. The findings suggest that antimicrobials in animal sector should be used more strictly, paying attention to critically important antimicrobials for humans and possible horizontal transfer of pathogens and their genetic determinants.

Prevalence, abundance, serovars and antimicrobial resistance of Salmonella isolated from retail raw poultry meat in China

2020 ◽  
Vol 713 ◽  
pp. 136385 ◽  
Author(s):  
Xiaojuan Yang ◽  
Jiahui Huang ◽  
Youxiong Zhang ◽  
Shengrong Liu ◽  
Ling Chen ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Poultry Meat ◽  
Raw Poultry

scholarly journals Antibiotic susceptibility and prevalence of foodborne pathogens in poultry meat in Romania

2015 ◽  
Vol 9 (01) ◽  
pp. 035-041 ◽  
Author(s):  
Sorin Daniel Dan ◽  
Alexandra Tabaran ◽  
Liora Mihaiu ◽  
Marian Mihaiu
Keyword(s):  
Antimicrobial Resistance ◽  
Campylobacter Jejuni ◽  
Resistance Genes ◽  
Foodborne Pathogens ◽  
Pathogenic Bacteria ◽  
Multidrug Resistant ◽  
Poultry Meat ◽  
Continuous Increase ◽  
E Coli ◽  
Microbiological Methods

Introduction: The occurrence of pathogenic strains in poultry meat is of growing concern in Romania. Another problem found on a global level is the continuous increase of antimicrobial resistance in bacteria isolated from food. This study aimed to evaluate the prevalence of pathogenic bacteria in poultry carcasses obtained in Romania in 2012–2013 and to reveal the most prevalent patterns of antimicrobial resistance in the isolated strains. Methodology: A total of 144 broiler chicken carcasses were evaluated according to classical microbiological methods. The DNA was extracted from the bacterial colonies and the resistance genes were identified by PCR. Results: In 2012, 47.2% of the samples revealed at least one of the following bacteria: Campylobacter jejuni (9.72%; n = 7), Salmonella enterica serotype Enteritidis (4.17%; n = 3), Listeria monocytogenes (15.28%; n = 11), and Escherichia coli (16.67%; n = 12). In 2013, the number of positive samples of pathogenic bacteria decreased, although Campylobacter jejuni was isolated in a higher percentage (20.8% vs. 9.72%). The percentage of multidrug-resistant (MDR) bacteria was high (23%); the most prevalent pattern included resistance to tetracycline, sulfonamides, and quinolones/fluoroquinolones. All the resistant Salmonella and E. coli strains were tested for the presence of characteristic resistance genes (Kn, blaTEM, tetA, tetB, tetG, DfrIa, aadA1a, Sul) and revealed that these isolates represent an important reservoir in the spread of this phenomenon. Conclusions: Our findings suggest that Romania urgently needs an integrated surveillance system within the entire chain, for drug-resistant pathogens isolated from poultry meat.

Molecular Characterization of Campylobacter jejuni in Raw Poultry Meat and Ready-to-Eat Poultry Meat Products in Northern India

2011 ◽  
Vol 1 (3) ◽  
pp. 135-140
Author(s):  
Gagan Sharma ◽  
R. Swaroop Rathore ◽  
U. Kumari Chauhan ◽  
R. Swaroop Chauhan Singh
Keyword(s):  
Campylobacter Jejuni ◽  
Control Point ◽  
Meat Product ◽  
Meat Products ◽  
Poultry Meat ◽  
Human Beings ◽  
Pcr Assay ◽  
Cultural Methods ◽  
Northern India ◽  
Raw Poultry

Campylobacter jejuni  Ã¢â‚¬Â to‐ eat poultry meat products have been foundimportant source of transmission of this organism on consumption. In thecurrent study,raw poultry and ready to eat poultry meat product samples atBareilly part of Northern India were tested for  C. jejuni. Total 660 samples ofpoultry meat and ready  Ã¢â‚¬Â to ‐eat poultry meat products were screened for thepresence of  C. jejuni using both cultural methods as well as PCR assay. Out ofthe total samples, 126 (19.09%) were confirmed positive by cultural methods,while 149 (22.50%) were confirmed positive by PCR assay. There wasalso variation in the prevalence of  C. jejuni amongst the various ready‐ toeatpoultry meat products. This study indicates the need for risk assessmentbased on microbiological criteria for  C .jejuni in poultry meat and ready‐ toeatpoultry meat products with strict implementation of provision of HazardAnalysis and Critical Control Point (HACCP) system. has been emerged as an important food borne zoonoticbacterial pathogen associated with gastric illness in human beings. Contaminatedpoultry and ready 

scholarly journals Whole genome-based characterisation of antimicrobial resistance and genetic diversity in Campylobacter jejuni and Campylobacter coli from ruminants

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Medelin Ocejo ◽  
Beatriz Oporto ◽  
José Luis Lavín ◽  
Ana Hurtado
Keyword(s):  
Genetic Diversity ◽  
Antimicrobial Resistance ◽  
Campylobacter Jejuni ◽  
Resistance Genes ◽  
Campylobacter Coli ◽  
Whole Genome ◽  
Northern Spain ◽  
Sequence Alignments ◽  
Phenotypic Data ◽  
Wide Range

AbstractCampylobacter, a leading cause of gastroenteritis in humans, asymptomatically colonises the intestinal tract of a wide range of animals.Although antimicrobial treatment is restricted to severe cases, the increase of antimicrobial resistance (AMR) is a concern. Considering the significant contribution of ruminants as reservoirs of resistant Campylobacter, Illumina whole-genome sequencing was used to characterise the mechanisms of AMR in Campylobacter jejuni and Campylobacter coli recovered from beef cattle, dairy cattle, and sheep in northern Spain. Genome analysis showed extensive genetic diversity that clearly separated both species. Resistance genotypes were identified by screening assembled sequences with BLASTn and ABRicate, and additional sequence alignments were performed to search for frameshift mutations and gene modifications. A high correlation was observed between phenotypic resistance to a given antimicrobial and the presence of the corresponding known resistance genes. Detailed sequence analysis allowed us to detect the recently described mosaic tet(O/M/O) gene in one C. coli, describe possible new alleles of blaOXA-61-like genes, and decipher the genetic context of aminoglycoside resistance genes, as well as the plasmid/chromosomal location of the different AMR genes and their implication for resistance spread. Updated resistance gene databases and detailed analysis of the matched open reading frames are needed to avoid errors when using WGS-based analysis pipelines for AMR detection in the absence of phenotypic data.

High Pressure Inactivation of Escherichia coli, Campylobacter jejuni, and Spoilage Microbiota on Poultry Meat

2012 ◽  
Vol 75 (3) ◽  
pp. 497-503 ◽  
Author(s):  
YANG LIU ◽  
MIRKO BETTI ◽  
MICHAEL G. GÄNZLE
Keyword(s):  
Escherichia Coli ◽  
High Pressure ◽  
Campylobacter Jejuni ◽  
Resistant Mutant ◽  
Poultry Meat ◽  
E Coli ◽  
Cell Counts ◽  
Meat Spoilage ◽  
Brochothrix Thermosphacta ◽  
Pressure Resistance

This study evaluated the high pressure inactivation of Campylobacter jejuni, Escherichia coli, and poultry meat spoilage organisms. All treatments were performed in aseptically prepared minced poultry meat. Treatment of 19 strains of C. jejuni at 300 MPa and 30°C revealed a large variation of pressure resistance. The recovery of pressure-induced sublethally injured C. jejuni depended on the availability of iron. The addition of iron content to enumeration media was required for resuscitation of sublethally injured cells. Survival of C. jejuni during storage of refrigerated poultry meat was analyzed in fresh and pressure-treated poultry meat, and in the presence or absence of spoilage microbiota. The presence of spoilage microbiota did not significantly influence the survival of C. jejuni. Pressure treatment at 400 MPa and 40°C reduced cell counts of Brochothrix thermosphacta, Carnobacterium divergens, C. jejuni, and Pseudomonas fluorescens to levels below the detection limit. Cell counts of E. coli AW1.7, however, were reduced by only 3.5 log (CFU/g) and remained stable during subsequent refrigerated storage. The resistance to treatment at 600 MPa and 40°Cof E. coli AW1.7 was compared with Salmonella enterica, Shiga toxin–producing E. coli and nonpathogenic E. coli strains, and Staphylococcus spp. Cell counts of all organisms except E. coli AW 1.7 were reduced by more than 6 log CFU/g. Cell counts of E. coli AW1.7 were reduced by 4.5 log CFU/g only. Moreover, the ability of E. coli AW1.7 to resist pressure was comparable to the pressure-resistant mutant E. coli LMM1030. Our results indicate that preservation of fresh meat requires a combination of high pressure with high temperature (40 to 60°C) or other antimicrobial hurdles.

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