Synergistic effects of synthesized iron nanoparticles of neem extract with conventional antibiotic against gram positive negative microorganism

Synthesis, Structure Elucidation, Antibacterial Activities, and Synergistic Effects of Novel Juglone and Naphthazarin Derivatives Against Clinical Methicillin-Resistant Staphylococcus aureus Strains

Frontiers in Chemistry ◽

10.3389/fchem.2021.773981 ◽

2021 ◽

Vol 9 ◽

Author(s):

Valentin Duvauchelle ◽

Chaimae Majdi ◽

David Bénimélis ◽

Catherine Dunyach-Remy ◽

Patrick Meffre ◽

...

Keyword(s):

Synergistic Effects ◽

Antibacterial Properties ◽

Clinical Importance ◽

Antibacterial Activities ◽

Multidrug Resistant ◽

Resistant Bacteria ◽

Global Public Health ◽

Gram Positive ◽

Drug Resistant Bacteria ◽

Mrsa Strains

Infections caused by drug-resistant bacteria are a serious threat to human and global public health. Moreover, in recent years, very few antibiotics have been discovered and developed by pharmaceutical companies. Therefore, there is an urgent need to discover and develop new antibacterial agents to combat multidrug-resistant bacteria. In this study, two novel series of juglone/naphthazarin derivatives (43 compounds) were synthesized and evaluated for their antibacterial properties against various clinical and reference Gram-positive MSSA, clinical Gram-positive MRSA, and clinical and reference Gram-negative bacteria E. coli and P. aeruginosa. These strains are of clinical importance because they belong to ESKAPE pathogens. Compounds 3al, 5ag, and 3bg showed promising activity against clinical and reference MSSA (MIC: 1–8 µg/ml) and good efficacy against clinical MRSA (MIC: 2–8 µg/ml) strains. 5am and 3bm demonstrated better activity on both MSSA (MIC: 0.5 µg/ml) and MRSA (MIC: 2 µg/ml) strains. Their MICs were similar to those of cloxacillin against clinical MRSA strains. The synergistic effects of active compounds 3al, 5ag, 5am, 3bg, and 3bm were evaluated with reference antibiotics, and it was found that the antibiotic combination with 3bm efficiently enhanced the antimicrobial activity. Compound 3bm was found to restore the sensitivity of clinical MRSA to cloxacillin and enhanced the antibacterial activity of vancomycin when they were added together. In the presence of 3bm, the MIC values of vancomycin and cloxacillin were lowered up to 1/16th of the original MIC with an FIC index of 0.313. Moreover, compounds 3al, 5ag, 5am, 3bg, and 3bm did not present hemolytic activity on sheep red blood cells. In silico prediction of ADME profile parameter results for 3bm is promising and encouraging for further development.

Synergistic effects of Persicaria odorata (Daun Kesom) leaf extracts with standard antibiotics on pathogenic bacteria

IIUM Medical Journal Malaysia ◽

10.31436/imjm.v16i2.321 ◽

2020 ◽

Vol 16 (2) ◽

Author(s):

P. M. Ridzuan ◽

Hairul Aini Hamzah ◽

Anis Shah ◽

Norazian Mohd Hassan ◽

Baharudin Roesnita

Keyword(s):

Pathogenic Bacteria ◽

Synergistic Effects ◽

Gas Chromatography Mass Spectrometry ◽

Diffusion Method ◽

Synergistic Activity ◽

Leaf Extracts ◽

Gram Positive ◽

Disk Diffusion Method ◽

Bacteria Growth ◽

Gram Positive Bacteria

Antibacterial activity of different types of P. odorata leaf extracts was evaluated in combination with standard antibiotics. Persicaria. odorata leaves were extracted with n-hexane (n-hex), dichloromethane (DCM) and methanol (MeOH). Each extract was applied on vancomycin (30µg), erythromycin (15µg) and gentamicin (10µg) discs, respectively. Disk diffusion method was used to evaluate the synergistic activity of each combination on Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Streptococcus pneumoniae, Pseudomonas aeruginosa, Salmonella typhi, and Escherichia coli. Minimum inhibitory concentration (MIC) and gas chromatography mass spectrometry (GCMS) analysis was performed on the active extract. Synergistic effects seen were mainly from the n-hex+antibiotics combinations, mainly on the Gram-positive bacteria (7 additive, 5 antagonistic), with MIC range from 50 µg/ml to 100 µg/ml, as well as Gram-negative bacteria (2 additive, 2 indifferent, 5 antagonistic). In particular, synergism showed by the combination of n-hex+van were all additive against the susceptible bacteria. DCM extract combination showed synergistic effects on three Gram-positive species (S. aureus, S. epidermidis, S. pyogenes). Meanwhile, MeOH+antibiotics combination showed significant additive synergistic effects (p<0.05) on S. aureus and S. epidermidis. The major compounds of leaves extract were decanal and β-citral. n-Hex extract superiorly inhibited Gram-positive bacteria growth as compared to DCM and MeOH extracts. The additive synergistic property of the n-hex P. odorata extract could be further studied for possible use as an antibacterial agent.

ANTIMICROBIAL ACTIVITY OF COPPER KAOLINITE AND SURFACTANT MODIFIED COPPER KAOLINITE AGAINST GRAM POSITIVE AND GRAM NEGATIVE BACTERIA

Jurnal Teknologi ◽

10.11113/jt.v78.7827 ◽

2016 ◽

Vol 78 (3-2) ◽

Author(s):

Nor Syafawani Sarah Md Saad ◽

Nik Ahmad Nizam Nik Malek ◽

Chun Shiong Chong

Keyword(s):

Antimicrobial Activity ◽

Synergistic Effects ◽

Wide Spectrum ◽

Copper Nitrate ◽

Gram Negative Bacteria ◽

Gram Positive ◽

Gram Negative ◽

X Ray ◽

Gram Positive Bacteria ◽

Diffusion Technique

The aim of this research was to determine the antimicrobial activity of kaolinite modified with antimicrobial compounds against Gram positive and Gram negative bacteria. Copper kaolinite (Cu-kaolinite) was prepared by loading raw kaolinite with copper nitrate trihydrate (CuNO3) while surfactant modified Cu-kaolinite (SM-Cu-kaolinite) was prepared by adding cationic surfactants hexadecyltrimethyl ammonium (HDTMA) on Cu-Kaolinite. Samples was characterized by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM) and energy dispersive X-ray (EDX) analyzer. The antimicrobial activity of the samples was tested against Gram negative bacteria (Escherichia coli ATCC 11229 and Pseudomonas aeruginosa ATCC 15442), and Gram positive bacteria (Staphylococcus aureus ATCC 6538 and Enterococcus faecalis ATCC 29212) through disc diffusion technique (DDT) and minimum inhibition concentration (MIC). The results showed that the antimicrobial activity of Cu-kaolinite increased after modified with HDTMA due to the synergistic effects of Cu ions and HDTMA molecules on the kaolinite. The antimicrobial activity for surfactant modified Cu-kaolinite was greater for Gram positive bacteria compared to Gram negative bacteria. In conclusion, the attachment of HDTMA on Cu-kaolinite contributed to the enhanced antimicrobial activity against wide spectrum of bacteria (Gram positive and Gram negative bacteria).

Assessment by Time-Kill Methodology of the Synergistic Effects of Oritavancin in Combination with Other Antimicrobial Agents against Staphylococcus aureus

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.00361-08 ◽

2008 ◽

Vol 52 (10) ◽

pp. 3820-3822 ◽

Cited By ~ 37

Author(s):

Adam Belley ◽

Eve Neesham-Grenon ◽

Francis F. Arhin ◽

Geoffrey A. McKay ◽

Thomas R. Parr ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Antimicrobial Agents ◽

Synergistic Effects ◽

Clinical Development ◽

Synergistic Activity ◽

Gram Positive ◽

Vancomycin Resistant ◽

Time Kill

ABSTRACT Oritavancin is a semisynthetic lipoglycopeptide in clinical development for serious gram-positive infections. This study describes the synergistic activity of oritavancin in combination with gentamicin, linezolid, moxifloxacin, or rifampin in time-kill studies against methicillin-susceptible, vancomycin-intermediate, and vancomycin-resistant Staphylococcus aureus.

Synergistic effects in Fe nanoparticles doped with ppm levels of (Pd + Ni). A new catalyst for sustainable nitro group reductions

Green Chemistry ◽

10.1039/c7gc02991h ◽

2018 ◽

Vol 20 (1) ◽

pp. 130-135 ◽

Cited By ~ 30

Author(s):

Haobo Pang ◽

Fabrice Gallou ◽

Hyuntae Sohn ◽

Jeffrey Camacho-Bunquin ◽

Massimiliano Delferro ◽

...

Keyword(s):

Synergistic Effect ◽

Nitro Group ◽

Iron Nanoparticles ◽

Synergistic Effects ◽

Fe Nanoparticles

A synergistic effect has been uncovered between ppm levels of Pd and Ni embedded within iron nanoparticles that leads to selective catalytic reductions of nitro-containing aromatics in water.

PSIX-20 Antibacterial activity of an essential oil combination against several common enteropathogens in swine production

Journal of Animal Science ◽

10.1093/jas/skaa054.326 ◽

2020 ◽

Vol 98 (Supplement_3) ◽

pp. 184-184

Author(s):

Bernat Canal ◽

Luis Mesas ◽

Cinta Sol ◽

Monica Puyalto ◽

Ana Carvajal ◽

...

Keyword(s):

Antibacterial Activity ◽

Synergistic Effects ◽

Bacterial Species ◽

Active Principle ◽

Swine Production ◽

Gram Positive ◽

Minimum Concentration ◽

Gram Negative ◽

E Coli ◽

Broth Microdilution Method

Abstract Essential oils (EOs) have different mechanisms, most of them targeting the bacterial wall. This fact can explain differences in the effectivity of EOs between Gram-positive and Gram-negative bacteria. Therefore, combining certain EOs can broaden their individual spectrum of efficacy due to potential synergistic effects. This trial aimed to test the in vitro antibacterial activity of an EO combination (oregano and clove oils) against a collection of relevant bacterial pathogens in swine production. The Gram-negative bacterial species chosen were Salmonella enterica ssp. enterica, Escherichia coli and Brachyspira hyodysenteriae and the Gram-positive bacterial species were Clostridium perfringens and Streptococcus suis. In addition, Lactobacillus fermentum was included to compare the susceptibility between this beneficial intestinal bacteria and the pathogens tested. The broth microdilution method at pH 6 and the subculturing from wells without bacterial growth were used to determine the minimum concentration of active principle necessary to inhibit (MIC) or kill (MBC) the 50% and 90% (MIC50/90/MBC50/90) of the population of every bacteria. The results showed that the lowest MIC50/90 were obtained for B. hyodysenteriae (37.5/75 ppm) while for S. enterica ssp. enterica (150/300 ppm), C. perfringens (150/150 ppm), E. coli (300/300 ppm) and S. suis (150/300 ppm) results were similar. Regarding the MBC50/90; B. hyodysenteriae (18.8/75 ppm) was the most susceptible pathogen, again, compared to S. enterica ssp. enterica (300/300 ppm), C. perfringens (150/150 ppm), E. coli (300/300 ppm) and S. suis (150/300 ppm). In contrast, the highest bacteriostatic/bactericidal concentrations were obtained against L. fermentum (MIC50/90 600/1,200 ppm and MBC50/90 600/2,400 ppm). These results suggest that the bacterial category (Gram-positive or Gram-negative) did not have an influence on the MIC and MBC. It can also be concluded that B. hyodysenteriae is the most susceptible enteropathogen to this EO blend. However, the in vivo effect of this combination of EOs must be further studied.

Synergistic Effects of Honeys and Commonly Used Antibiotics on Gram Positive Bacteria

Egyptian Academic Journal of Biological Sciences G Microbiology ◽

10.21608/eajbsg.2015.16491 ◽

2015 ◽

Vol 7 (1) ◽

pp. 101-109

Author(s):

Masoud A. ◽

Alqurashi M. ◽

Alamin A.

Keyword(s):

Synergistic Effects ◽

Gram Positive ◽

Gram Positive Bacteria

Antibacterial effect of Allium cepa (onion), Allium sativum (garlic), Azadirachta indica (neem) extract against different bacteria isolated from clinical sample.

The Professional Medical Journal ◽

10.29309/tpmj/2021.28.08.4733 ◽

2021 ◽

Vol 28 (08) ◽

pp. 1083-1089

Author(s):

Muhammad Shahbaz Hussain ◽

◽

Muhammad Bilal Ghafoor ◽

Umar Khalid Cheema ◽

Muhammad Sajjad ◽

...

Keyword(s):

Allium Cepa ◽

Aqueous Extract ◽

Azadirachta Indica ◽

Allium Sativum ◽

Diffusion Method ◽

Gram Negative Bacteria ◽

Zone Of Inhibition ◽

Gram Positive ◽

Neem Extract ◽

Gram Negative

Objective: The objective of this study was to evaluate and screen the antibacterial activity of medicinal plant which were most cultivated in ancient time. Study Design: Experimental Study. Setting: Microbiology Section, Pathology Department, Sheikh Zayed Hospital/Medical College Rahim Yar Khan. Period: February 2019 to May 2019. Material & Methods: The fresh aqueous extract of garlic cloves (Allium sativum), onion bulb (Allium cepa) and neem (Azadirachta indica) leaves used against the clinically isolated Staphylococcus aureus(S.aureus), Escherichia coli (E.coli), Klebsiella, Acinetobacter and Pseudomonas species by using agar well diffusion method. Aqueous extract of used plant tested at different concentrations of 100%, 75%, 50%, 25%. Results: All these concentrations showed inhibitory effect towards Gram positive bacteria (S.aureus), while Gram negative bacteria more sensitive to higher concentration. In this analysis, on comparison we observed that fresh garlic juice extract was more efficient on all tested bacteria either Gram positive or Gram negative than other and give maximum zone of inhibition on S.aureus (40mm) due to their high chemistry. Gram negative bacteria mainly E. coli somehow resistant to onion and neem extract because they possess the ability to rapidly mutate their genetic makeup towards phytochemical compounds. Conclusion: The result of study also elicits that efficacy of fresh extract depends upon concentrations levels. The plant showed maximum zone of inhibition at 100% than at 25% because on higher level they contain higher bioactive compounds.

Antimicrobial effects of zero-valent iron nanoparticles on gram-positive Bacillus strains and gram-negative Escherichia coli strains

Journal of Nanobiotechnology ◽

10.1186/s12951-017-0314-1 ◽

2017 ◽

Vol 15 (1) ◽

Cited By ~ 19

Author(s):

Yi-Huang Hsueh ◽

Ping-Han Tsai ◽

Kuen-Song Lin ◽

Wan-Ju Ke ◽

Chao-Lung Chiang

Keyword(s):

Escherichia Coli ◽

Iron Nanoparticles ◽

Zero Valent Iron ◽

Gram Positive ◽

Gram Negative ◽

Antimicrobial Effects ◽

Zero Valent Iron Nanoparticles ◽

Bacillus Strains

Antibacterial efficacy of black seed honey in combination with penicillin and amoxiclav against gram-positive bacteria

International Journal of Scientific Reports ◽

10.18203/issn.2454-2156.intjscirep20200196 ◽

2020 ◽

Vol 6 (2) ◽

pp. 61

Author(s):

Rozely Hossain ◽

M. S. Rahman ◽

M. A. Rayhan ◽

Kashfia Nawrin ◽

Mohammad M. Billah ◽

...

Keyword(s):

Synergistic Effects ◽

Micrococcus Luteus ◽

Diffusion Method ◽

Resistant Bacteria ◽

Antibacterial Efficacy ◽

Gram Positive ◽

Gram Positive Bacteria ◽

Black Seed ◽

Microdilution Method ◽

Zones Of Inhibition

Background: The emergence of antimicrobial resistance possesses a great threat for the existence of mankind. Antibiotics like penicillin and amoxiclav are at the brink of losing their efficacy entirely in exposure to resistant bacteria. Thus, the present study was aimed to find out the antibacterial efficacy of black seed honey as an alternative natural source which can act independently and boost the efficacy of standard drugs alongside.Methods: Penicillin, amoxiclav and black seed honey were first individually trailed against four gram-positive bacteria - Bacillus subtilis, Staphylococcus aureus, Staphylococcus epidermidis and Micrococcus luteus. Afterwards, penicillin and amoxiclav were used in combination with honey and compared the synergistic effects with their individual efficacy. Zones of inhibition from well diffusion method, percentage inhibition, minimum inhibitory and bactericidal concentrations by microdilution method were determined in the present study. Results: Black seed honey alone demonstrated great inhibitory potential against S. aureus (9.7 mm), S. epidermidis (9.9 mm) and M. luteus (9.3 mm) in well diffusion method. Moreover, its combination with amoxiclav showed synergistic effect against all bacteria except S. epidermidis. However, its conjugation with penicillin was not able to produce any synergism as exhibited by zones of inhibition. The lowest concentration (1.56%) of honey applied individually or in combination in microdilution method found highly effective which established an inverse dose dependent relationship with efficacy.Conclusions: From the data it can be concluded that the black seed honey is a highly potent natural agent which can be utilized in antimicrobial therapy. However, further investigation is recommended to identify the responsible compound for such activity.