Transient manipulation of the expression level of selected growth rate correlating microRNAs does not increase growth rate in CHO-K1 cells

2019 ◽  
Vol 295 ◽  
pp. 63-70
Author(s):  
Gerald Klanert ◽  
Nina Bydlinski ◽  
Patrice Agu ◽  
Andreas B. Diendorfer ◽  
Matthias Hackl ◽  
...  
Keyword(s):  
Growth Rate ◽  
Expression Level ◽  
Increase Growth Rate

scholarly journals In vitro culture as an aid to conservation of indigenous ferns: Diplazium proliferum

2015 ◽  
Vol 6 (1) ◽  
Author(s):  
Zubeir M. Golamaully ◽  
Vishwakalyan Bhoyroo ◽  
Nadeem Nazurally ◽  
Vineshwar Gopal
Keyword(s):  
Growth Rate ◽  
In Vitro Culture ◽  
Mercuric Chloride ◽  
Rare Species ◽  
Vascular Plants ◽  
Culture Media ◽  
Fungal Contamination ◽  
Increase Growth Rate ◽  
Growing Population

With the ever growing population and economic needs of Mauritius, the flora of Mauritius has never been in more danger and one group of vascular plants is even more in peril; ferns.<em> Diplazium proliferum</em> is indigenous to the Mascarene region and is considered as a rare species in Mauritius. The need to develop a tested <em>in vitro</em> propagation protocol is a must to protect the biodiversity of Mauritius. This experiment was geared towards the establishment of a proper sterilization technique and the effect of 6-benzylaminopurine (BAP) and light on <em>in vitro</em> culture of this fern. Sterilization with 0.05% Mercuric chloride was effective to eliminate fungal contamination and allow germination of spores. Culture media supplemented with BAP did not significantly increase growth rate of both gametophytes and sporophytes of<em> D. proliferum</em>. Present results suggest efficient sterilization methods to be a crucial stage for successful<em> in vitro r</em>egeneration of ferns. The established protocol will be used as an optimized baseline protocol for the propagation of other indigenous ferns.

Feed Additives and Fish Meal for Growing Cattle

1986 ◽  
Vol 1986 ◽  
pp. 114-114
Author(s):  
S. Thomas ◽  
C.K. Mackie ◽  
E.A. Hunter
Keyword(s):  
Growth Rate ◽  
Dietary Supplements ◽  
Fish Meal ◽  
Feed Additives ◽  
Increase Growth Rate ◽  
Grazing Season ◽  
Growing Cattle ◽  
Ad Libitum

White fish meal and feed additives are used to increase growth rate by young growing cattle in their first winter before being turned out to graze. Some evidence indicates that increased gains may not be retained through the grazing season. A trial was made to measure the effect of fish meal and feed additives, alone or in combination, on performance by young growing cattle during winter and subsequently through the grazing season. Sixty British Friesian castrated male calves were offered silage containing 220g/kg DM ad libitum. There were six dietary supplements.

Surface Morphologies and Optical Properties of Homoepitaxial ZnO Grown by Close-Spaced Chemical Vapor Transport

2007 ◽  
Vol 1012 ◽  
Author(s):  
Koji Abe ◽  
Tetsuya Tokuda ◽  
Yuta Banno ◽  
Osamu Eryu
Keyword(s):  
Growth Rate ◽  
Chemical Vapor ◽  
Vapor Transport ◽  
Chemical Vapor Transport ◽  
Powder Surface ◽  
Increase Growth Rate ◽  
Homoepitaxial Growth ◽  
Surface Morphologies ◽  
Substrate Temperatures

AbstractChemical vapor transport (CVT) using carbon as a transporting agent is studied for homoepitaxial growth on O-polar ZnO substrates. To increase growth rate at high temperatures, we keep a substrate close to ZnO source powder. Surface smoothness and crystal quality of epilayers are remarkably improved by increasing a substrate temperature. Smooth surfaces are observed on the epilayer grown at substrate temperatures above 920°C.

GROWTH RETARDATION EFFECTS OF SOME GLUCOCORTICOIDS AND GLUCAGON IN CHICKENS

1970 ◽  
Vol 50 (3) ◽  
pp. 629-637 ◽  
Author(s):  
J. S. GAVORA ◽  
P. A. KONDRA
Keyword(s):  
Growth Rate ◽  
Body Weight ◽  
Weight Gain ◽  
Oral Administration ◽  
Growth Retardation ◽  
Broiler Chickens ◽  
Body Weight Gain ◽  
Liver Weight ◽  
Hydrocortisone Acetate ◽  
Increase Growth Rate

Three experiments were conducted to test the effects of Lipo-adrenal cortex (LAC), cortisone acetate (CA), hydrocortisone acetate (HCA) and glucagon (G) in broiler chickens. Oral administration of 38.7, 69.6 or 96.8 mg CA per bird significantly retarded body weight gain between days 14 and 21. HCA administered intramuscularly in doses varying from 2 to 24 mg/100 g of body weight significantly decreased body weight gain between days 14 and 28. Shank growth was similarly affected by doses over 4 mg HCA. The same doses significantly increased liver weight. At 3 months of age, birds recovered from growth retardation caused by the injection of 4 mg HCA/100 g of body weight at 14 days of age. Experimental results indicate that 2 or 4 mg HCA/100 g of body weight, administered by one injection at 14 days of age, may be used to increase growth rate variance by retarding growth.

scholarly journals Insulin-like growth factor-I and analogues increase growth in artificially-reared neonatal pigs

2002 ◽  
Vol 87 (6) ◽  
pp. 587-593 ◽  
Author(s):  
Frank R. Dunshea ◽  
Chung S. Chung ◽  
Phil C. Owens ◽  
John F. Ballard ◽  
Paul E. Walton
Keyword(s):  
Growth Rate ◽  
Growth Factors ◽  
Weight Gain ◽  
Milk Intake ◽  
Daily Weight Gain ◽  
Initial Weight ◽  
Increase Growth Rate ◽  
Neonatal Pigs ◽  
Igf I ◽  
Ad Libitum

Exogenous insulin-like growth factor (IGF)-I has been shown to increase growth rate in neonatal pigs while an analogue of IGF-I, long arginine (LR3) IGF-I, has been shown to be more potent than IGF-I in the rat. Therefore, two studies were conducted to determine whether IGF-I and LR3IGF-I increase growth in the artificially-reared neonatal pig. Expt 1 involved forty-two (2 kg initial weight) pigs infused with either control, IGF-I (2, 4 or 8 μg/h) or LR3IGF-I (2, 4 or 8 μg/h) infusions for 8 d. Pigs were weighed and then offered 1·7 MJ (gross energy) milk replacer/kg0·75 per d. Expt 2 involved eighteen pigs (2 kg initial weight) treated with control saline, IGF-I (8 μg/h) or LR3IGF-I (8 μg/h) infusions. After 9 d an additional pump was inserted to increase the infusion rates of each of the growth factors (16 μg/h) for a further 9 d. Cows' milk was provided ad libitum. In Expt 1 there was no overall effect of growth factors on daily weight gain or slaughter weight. However, milk intake was greater in pigs infused with growth factors (909 v. 867 g/d, P=0·027), with an apparently greater milk intake by the pigs infused with IGF-I compared with LR3IGF-I (920 v. 898 g/d, P=0·12). Infusion of LR3IGF-I decreased plasma IGF-I concentrations, but had no effect on plasma IGF-II concentrations. In Expt 2, neither IGF-I nor LR3IGF-I infusion had any effect upon daily weight gain over the first 9 d of the study. However, over the second 9 d of the study, daily weight gain was increased in LR3IGF-I-infused pigs (457 v. 386 g/d, P<0·01), but not in pigs infused with IGF-I (413 v. 386 g/d, P=0·15). Milk intake was not different during the first 9 d of the study but was significantly greater in pigs infused with growth factors over the second half of the study (3407 v. 2905 g/d, P<0·01). Plasma IGF-binding protein-3 concentrations were highly correlated (R=0·85) with average daily gain over the 3 d preceding blood sampling. In conclusion, exogenous IGF-I and particularly LR3IGF-I can increase growth rate and milk intake in artificially-reared pigs fed ad libitum but not in limit-fed piglets.

scholarly journals Recovery of Recombinant Avian Paramyxovirus Type-3 Strain Wisconsin by Reverse Genetics and Its Evaluation as a Vaccine Vector for Chickens

Viruses ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 316
Author(s):  
Mohamed A. Elbehairy ◽  
Sunil K. Khattar ◽  
Siba K. Samal
Keyword(s):  
Growth Rate ◽  
Tissue Distribution ◽  
Vaccine Vector ◽  
Expression Level ◽  
Reverse Genetic System ◽  
Foreign Protein ◽  
Foreign Gene ◽  
Avian Paramyxovirus ◽  
Gfp Expression ◽  
Type 3

A reverse genetic system for avian paramyxovirus type-3 (APMV-3) strain Wisconsin was created and the infectious virus was recovered from a plasmid-based viral antigenomic cDNA. Green fluorescent protein (GFP) gene was cloned into the recombinant APMV-3 genome as a foreign gene. Stable expression of GFP by the recovered virus was confirmed for at least 10 consecutive passages. APMV-3 strain Wisconsin was evaluated against APMV-3 strain Netherlands and APMV-1 strain LaSota as a vaccine vector. The three viral vectors expressing GFP as a foreign protein were compared for level of GFP expression level, growth rate in chicken embryo fibroblast (DF-1) cells, and tissue distribution and immunogenicity in specific pathogen-free (SPF) day-old chickens. APMV-3 strain Netherlands showed highest growth rate and GFP expression level among the three APMV vectors in vitro. APMV-3 strain Wisconsin and APMV-1 strain LaSota vectors were mainly confined to the trachea after vaccination of day-old SPF chickens without any observable pathogenicity, whereas APMV-3 strain Netherlands showed wide tissue distribution in different body organs (brain, lungs, trachea, and spleen) with mild observable pathogenicity. In terms of immunogenicity, both APMV-3 strain-vaccinated groups showed HI titers two to three fold higher than that induced by APMV-1 strain LaSota vaccinated group. This study offers a novel paramyxovirus vector (APMV-3 strain Wisconsin) which can be used safely for vaccination of young chickens as an alternative for APMV-1 strain LaSota vector.

scholarly journals Optimized expression of Hfq protein increases Escherichia coli growth

2021 ◽  
Vol 15 (1) ◽  
Author(s):  
Phuong N. L. VO ◽  
Hyang-Mi LEE ◽  
Jun REN ◽  
Dokyun NA
Keyword(s):  
Escherichia Coli ◽  
Growth Rate ◽  
Stress Responses ◽  
Bacterial Species ◽  
Expression Level ◽  
Rna Seq ◽  
E Coli ◽  
Network Analyses ◽  
Metabolic Genes ◽  
Cell Densities

AbstractEscherichia coli is a widely used platform for metabolic engineering due to its fast growth and well-established engineering techniques. However, there has been a demand for faster-growing E. coli for higher production of desired substances. Here, to increase the growth of E. coli cells, we optimized the expression level of Hfq protein, which plays an essential role in stress responses. Six variants of the hfq gene with a different ribosome binding site sequence and thereby a different expression level were constructed. When the Hfq expression level was optimized in DH5α, its growth rate was increased by 12.1% and its cell density was also increased by 4.5%. RNA-seq and network analyses revealed the upregulation of stress response genes and metabolic genes, which increases the tolerance against pH changes. When the same strategy was applied to five other E. coli strains (BL21 (DE3), JM109, TOP10, W3110, and MG1655), all their growth rates were increased by 18–94% but not all their densities were increased (− 12 − + 32%). In conclusion, the Hfq expression optimization can increase cell growth rate and probably their cell densities as well. Since the hfq gene is highly conserved across bacterial species, the same strategy could be applied to other bacterial species to construct faster-growing strains.

scholarly journals The Effect of miR-539 Regulating TRIAP1 on the Apoptosis, Proliferation, Migration and Invasion of Osteosarcoma Cells

2021 ◽  
Author(s):  
Huowen Liu ◽  
Min Yang ◽  
Yufeng Zhang ◽  
Zhiqiang Yang ◽  
Zhe Chen ◽  
...  
Keyword(s):  
Growth Rate ◽  
Western Blot ◽  
Expression Level ◽  
Luciferase Reporter ◽  
Migration And Invasion ◽  
Control Group ◽  
Osteosarcoma Cells ◽  
Migration Ability ◽  
Transfection Efficacy

Abstract Objective The purpose of this study is to explore the effect of miRNA-539 on osteosarcoma and the underlying mechanism, so as to find a new method for early diagnosis and treatment of osteosarcoma.Method miRNA-539 mimics was transfected into osteosarcoma cells 143b and MG-63 and upregulated the expression of miR-539. QT-PCR was used to detect transfection efficacy. CCK-8 method was used to detect proliferation of 143b and MG-63 osteosarcoma cells and flow cytometry was used to detect the apoptosis of osteosarcoma cells 143b and MG-63. Wound-healing test and Transwell test were used to detect the migration and invasion ability of osteosarcoma cells. TRIAP1 was found to be the potential target gene of miRNA-539 by online bioinformatics software and the expression level of TRIAP1 in osteosarcoma cells overexpressing miRNA-539 was detected by qT-PCR. Western blot was used to detect the level of expression of TRIAP1 and its downstream genes (p53, p21, apaf1 and caspase9) in osteosarcoma cells 143b and MG63 transfected with miR-539 mimics or miR-539 mimics-NC. A model of osteosarcoma subcutaneously transplanted in nude mice was constructed to observe the effect of miRNA-539 on the growth rate of osteosarcoma in vivo.Results After transfection of miRNA-539 mimics in osteosarcoma cells 143b and MG63, the proliferation level, migration ability, and invasion ability of the osteosarcoma cells were significantly lower than that in the control group, and the apoptosis level was significantly higher than that in the control group (P <0.01). The dual luciferase reporter confirmed that TRIAP1 was the target of miR-539, and the expression level of TRIAP1 in 143b and MG63 transfected with miRNA-539 mimics was proved to be significantly lower than that in the control group (P<0.01).The western blot showed the expression of genes targeted by TRIAP1 was upregulated when the expression of TRIAP1 was downregulated. In vivo, the osteosarcoma growth rate in the miRNA-539 mimics group was significantly slower than that in the control group (P<0.01).Conclusion MiRNA-539 may inhibit the cell proliferation, migration and invasion of osteosarcoma cells and promote the apoptosis of osteosarcoma cells by targeting on TRIAP1.

scholarly journals The Effect of Immersion Duration in Thyroxine Hormone on Growth of Snakehead Fish Larvae (Channa striata)

2019 ◽  
Vol 4 (1) ◽  
Author(s):  
Muslim Muslim ◽  
Ade Dwi Sasanti ◽  
Apriana Apriana
Keyword(s):  
Growth Rate ◽  
Fish Larvae ◽  
Fish Larva ◽  
Increase Growth Rate ◽  
Average Value ◽  
Fish Breeding ◽  
Randomized Design ◽  
Channa Striata ◽  
Completely Randomized Design ◽  
Research Show

The aim of this research was to determine the best immertion duration to increase growth rate of snakehead fish larva. The research was conducted in Fish Breeding unit Batanghari Sembilan Indralaya, Kabupaten Ogan Ilir. This research used a Completely Randomized Design (CRD), with five treatments (0, 12, 24, 36, and 48 hours of immertion), with three replications. The parameters observed were growth, survival rate and water quality. The results of  this research show that the highest growth rate found in the 24 hours of treatment  with the average value of 0.17 g of weight and increase in length of 1.90 cm. The best survival contained in treatment of immersion for 36 hours with the average value of 71.67%.

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