The response of nitrogen cycling and bacterial communities to E. coli invasion in aquatic environments with submerged vegetation

AbstractAquatic environments are key niches for the emergence, evolution and dissemination of antimicrobial resistance. However, the population diversity and the genetic elements that drive the dynamics of resistant bacteria in different aquatic environments are still largely unknown. The aim of this study was to understand the population genomics and evolutionary events of Escherichia coli resistant to clinically important antibiotics including aminoglycosides, in anthropogenic and natural water ecosystems. Here we show that less different E. coli sequence types (STs) are identified in wastewater than in rivers, albeit more resistant to antibiotics, and with significantly more plasmids/cell (6.36 vs 3.72). However, the genomic diversity within E. coli STs in both aquatic environments is similar. Wastewater environments favor the selection of conserved chromosomal structures associated with diverse flexible plasmids, unraveling promiscuous interplasmidic resistance genes flux. On the contrary, the key driver for river E. coli adaptation is a mutable chromosome along with few plasmid types shared between diverse STs harboring a limited resistance gene content.

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Comparative efficacy of five different rep-PCR methods to discriminate Escherichia coli populations in aquatic environments

Water Science & Technology ◽

10.2166/wst.2008.424 ◽

2008 ◽

Vol 58 (3) ◽

pp. 537-547 ◽

Cited By ~ 38

Author(s):

B. R. Mohapatra ◽

A. Mazumder

Keyword(s):

Cluster Analysis ◽

Disease Transmission ◽

Management Practices ◽

Gastrointestinal Disease ◽

Function Analysis ◽

Average Rate ◽

Aquatic Environments ◽

Correct Classification ◽

Comparative Efficacy ◽

E Coli

Development of efficient techniques to discriminate the sources of E. coli in aquatic environments is essential to improve the surveillance of fecal pollution indicators, to develop strategies to identify the sources of fecal contamination, and to implement appropriate management practices to minimize gastrointestinal disease transmission. In this study the robustness of five different rep-PCR methods, such as REP-PCR, ERIC-PCR, ERIC2-PCR, BOX-PCR and (GTG)5-PCR were evaluated to discriminate 271 E. coli strains isolated from two watersheds (Lakelse Lake and Okanagan Lake) located in British Columbia, Canada. Cluster analysis of (GTG)5-PCR, BOX-PCR, REP-PCR, ERIC-PCR and ERIC2-PCR profiles of 271 E. coli revealed 43 clusters, 35 clusters, 28 clusters, 23 clusters and 14 clusters, respectively. The discriminant analysis of rep-PCR genomic fingerprints of 271 E. coli isolates yielded an average rate of correct classification (watershed-specific) of 86.8%, 82.3%, 78.4%, 72.6% and 55.8% for (GTG)5-PCR, BOX-PCR, REP-PCR, ERIC-PCR and ERIC2-PCR, respectively. Based on the results of cluster analysis and discriminant function analysis, (GTG)5-PCR was found to be the most robust molecular tool for differentiation of E. coli populations in aquatic environments.

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Spread of Antibiotic Resistance in Aquatic Environments: E. coli as a Case Study

Proceedings ◽

10.3390/proceedings2110693 ◽

2018 ◽

Vol 2 (11) ◽

pp. 693 ◽

Cited By ~ 1

Author(s):

Maria Adamantia Efstratiou ◽

Marina Bountouni ◽

Efthimios Kefalas

Keyword(s):

Escherichia Coli ◽

Drinking Water ◽

Antibiotic Resistance ◽

Rural Communities ◽

Ground Water ◽

Aquatic Environments ◽

Multiple Resistance ◽

E Coli ◽

Of Greece

The aim of this study was to gather information on the spread of antibiotic resistance in Escherichia coli isolates from wells, boreholes and untreated drinking water in islands of Greece. We analyzed for antibiotic resistance 235 E. coli strains isolated from untreated drinking water of small rural communities, and ground water from 4 islands. Resistance was tested against Norfloxacin, Ciprofloxacin, Levofloxacin, Amoxicillin and Cefaclor. More than half (54.9%) were resistant to at least one of the antibiotics tested. Of these 26.3% showed multiple resistance (to two or more antibiotics). Strains from drinking water sources were overall more sensitive. Frequent resistance was observed for Amoxicillin (38.3%) and Levofloxacin (28.5%), low for Norfloxacin (5.5%).

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Analysis of Bacterial Communities on North Sea Macroalgae and Characterization of the Isolated Planctomycetes Adhaeretor mobilis gen. nov., sp. nov., Roseimaritima multifibrata sp. nov., Rosistilla ulvae sp. nov. and Rubripirellula lacrimiformis sp. nov.

Microorganisms ◽

10.3390/microorganisms9071494 ◽

2021 ◽

Vol 9 (7) ◽

pp. 1494

Author(s):

Sandra Wiegand ◽

Patrick Rast ◽

Nicolai Kallscheuer ◽

Mareike Jogler ◽

Anja Heuer ◽

...

Keyword(s):

North Sea ◽

Bacterial Communities ◽

Aquatic Environments ◽

The North ◽

The North Sea ◽

Epiphytic Communities ◽

Different Strains ◽

A Minor ◽

The Phylum Planctomycetes

Planctomycetes are bacteria that were long thought to be unculturable, of low abundance, and therefore neglectable in the environment. This view changed in recent years, after it was shown that members of the phylum Planctomycetes can be abundant in many aquatic environments, e.g., in the epiphytic communities on macroalgae surfaces. Here, we analyzed three different macroalgae from the North Sea and show that Planctomycetes is the most abundant bacterial phylum on the alga Fucus sp., while it represents a minor fraction of the surface-associated bacterial community of Ulva sp. and Laminaria sp. Especially dominant within the phylum Planctomycetes were Blastopirellula sp., followed by Rhodopirellula sp., Rubripirellula sp., as well as other Pirellulaceae and Lacipirellulaceae, but also members of the OM190 lineage. Motivated by the observed abundance, we isolated four novel planctomycetal strains to expand the collection of species available as axenic cultures since access to different strains is a prerequisite to investigate the success of planctomycetes in marine environments. The isolated strains constitute four novel species belonging to one novel and three previously described genera in the order Pirellulales, class Planctomycetia, phylum Planctomycetes.

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Escherichia coli is not a suitable fecal indicator to assess water fecal contamination by otters

Brazilian Journal of Biology ◽

10.1590/1519-6984.167279 ◽

2017 ◽

Vol 78 (1) ◽

pp. 155-159 ◽

Cited By ~ 2

Author(s):

M. Oliveira ◽

D. Freire ◽

N. M. Pedroso

Keyword(s):

Escherichia Coli ◽

Fecal Contamination ◽

Microbiological Quality ◽

Aquatic Environments ◽

Pathogenic Microorganisms ◽

Fecal Indicator ◽

Fecal Samples ◽

E Coli ◽

Paulo State

Abstract The detection of pathogenic microorganisms in aquatic environments is extremely relevant in terms of public health. As these laboratorial methodologies are usually difficult, expensive and time-consuming, they are frequently replaced by the assessment of fecal indicator bacteria, such as Escherichia coli. This study aimed to assess the presence of E. coli in fecal samples from Neotropical otters, to evaluate its potential as fecal indicator to be applied to the determination of water microbiological quality in areas where otters’ populations are high. Twenty-six otter fecal samples, collected in Alto Paranapanema river basin, São Paulo State, Brazil, were analyzed for the presence of E. coli, using conventional bacteriological methods. Only 8 scat samples (30%) were E. coli positive, indicating that this microorganism is not a suitable fecal indicator to assess water fecal contamination by Neotropical otters, and should not be used to infer the presence of otter related pathogens in waters.

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Contrasting microbiota profiles observed in children carrying either Blastocystis spp. or the commensal amoebas Entamoeba coli or Endolimax nana

Scientific Reports ◽

10.1038/s41598-020-72286-y ◽

2020 ◽

Vol 10 (1) ◽

Cited By ~ 1

Author(s):

Juan F. Alzate ◽

Miguel Toro-Londoño ◽

Felipe Cabarcas ◽

Gisela Garcia-Montoya ◽

Ana Galvan-Diaz

Keyword(s):

Bacterial Communities ◽

Intestinal Parasites ◽

Intestinal Lumen ◽

Bacterial Populations ◽

Microbial Competition ◽

E Coli ◽

Daycare Centers ◽

Microbial Profile ◽

Bacterial Microbiota ◽

Bacterial Richness

Abstract Recent studies have shown how intestinal parasites can modulate gut microbiota. This observation is not surprising since the human intestinal lumen, like any other niche, is a battlefield of microbial competition, and Eukaryotes can affect bacterial populations. Intestinal pathogenic protist has been associated with reshaping the microbial community structure; however, the interactions between the colonic bacterial communities and parasites like Blastocystis spp., Entamoeba coli, and Endolimax nana have been poorly studied. In this work, we studied the distal intestinal bacterial microbiota of 49 children attending 7 public daycare centers in Medellin, Colombia, and compared the bacterial microbiota structure in the presence or absence of the protists Blastocystis spp., E. coli, and E. nana. Parasite colonization was associated with an increase in bacterial richness. Moreover, Blastocystis spp. presented a positive relationship with Prevotella, since this bacterium was selectively enriched in children carrying it. Remarkably, the E. coli colonized children showed a microbial profile that was closer to uninfected controls, although some bacterial taxa displayed to be enriched. This is the case for Akkermansia, which showed to be favored in E. coli colonized individuals, while notably reduced in the Blastocystis spp. parasitized group.

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Impact of wildfire intensity and logging on fungal and nitrogen-cycling bacterial communities in British Columbia forest soils

Forest Ecology and Management ◽

10.1016/j.foreco.2010.05.037 ◽

2010 ◽

Vol 260 (5) ◽

pp. 787-794 ◽

Cited By ~ 10

Author(s):

Nabla Kennedy ◽

Keith N. Egger

Keyword(s):

British Columbia ◽

Nitrogen Cycling ◽

Forest Soils ◽

Bacterial Communities

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Actinomycetes in the Elbow River Basin, Alberta, Canada

Water Quality Research Journal ◽

10.2166/wqrj.2003.007 ◽

2003 ◽

Vol 38 (1) ◽

pp. 115-125 ◽

Cited By ~ 13

Author(s):

Beryl Zaitlin ◽

Susan B. Watson ◽

Jamieson Dixon ◽

Deborah Steel

Keyword(s):

Drinking Water ◽

Agricultural Land ◽

Water Source ◽

Aquatic Environments ◽

Drinking Water Source ◽

E Coli ◽

Stream Discharge ◽

Stream Characteristics ◽

The Relationship ◽

The City

Abstract Actinomycetes can produce significant amounts of the earthy-muddy odour compounds geosmin and 2-methylisoborneol (MIB). These filamentous bacteria are found in both terrestrial and aquatic environments, and are particularly abundant in soil. They can enter freshwater systems via terrestrial runoff and subsequently cause taste and odour outbreaks in drinking water. Since it is well known that actinomycete growth and odour production is modified strongly by environmental factors such as moisture and nutrient levels, we hypothesized that watershed and stream characteristics should influence the potential odour impact of soil runoff on surface water. In this study, 1) the relationship between actinomycete abundance and characteristics such as stream discharge, turbidity and Escherichia coli levels was investigated, and 2) actinomycetes from contrasting terrestrial sources were examined for differences in their geosmin and MIB production. Actinomycetes and stream characteristics were sampled from the Elbow River, an important drinking water source for the City of Calgary (Alberta, Canada), and three tributary streams. Actinomycetes from forested regions and agricultural land were tested for taste and odour compound production. Actinomycete levels in streams were found to correlate closely with E. coli levels and to a lesser extent with turbidity, suggesting that actinomycetes are particularly abundant in runoff from terrestrial sources with fecal contamination. Most of the 18 actinomycete isolates tested were able to produce geosmin and/or MIB regardless of their terrestrial sources, suggesting that taste and odour outbreaks due to actinomycetes may be more influenced by differences in abundance than differences in source.

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Changes of Bacterial Communities in Response to Prolonged Hydrodynamic Disturbances in the Eutrophic Water-Sediment Systems

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph16203868 ◽

2019 ◽

Vol 16 (20) ◽

pp. 3868

Author(s):

Haomiao Cheng ◽

Ling Cheng ◽

Liang Wang ◽

Tengyi Zhu ◽

Wei Cai ◽

...

Keyword(s):

Relative Abundance ◽

Bacterial Communities ◽

16S Rrna Gene Sequencing ◽

Aquatic Environments ◽

Rrna Gene ◽

Evolutionary Trend ◽

Ecological Evaluation ◽

Eutrophic Water ◽

Rrna Gene Sequencing ◽

Opposite Tendency

The effects of hydrodynamic disturbances on the bacterial communities in eutrophic aquatic environments remain poorly understood, despite their importance to ecological evaluation and remediation. This study investigated the evolution of bacterial communities in the water–sediment systems under the influence of three typical velocity conditions with the timescale of 5 weeks. The results demonstrated that higher bacterial diversity and notable differences were detected in sediment compared to water using the 16S rRNA gene sequencing. The phyla Firmicutes and γ-Proteobacteria survived better in both water and sediment under stronger water disturbances. Their relative abundance peaked at 36.0%, 33.2% in water and 38.0%, 43.6% in sediment, respectively, while the phylum Actinobacteria in water had the opposite tendency. Its relative abundance grew rapidly in static control (SC) and peaked at 44.8%, and it almost disappeared in disturbance conditions. These phenomena were caused by the proliferation of genus Exiguobacterium (belonging to Firmicutes), Citrobacter, Acinetobacter, Pseudomonas (belonging to γ-Proteobacteria), and hgcI_clade (belonging to Actinobacteria). The nonmetric multidimensional scaling (NMDS) and Venn analysis also revealed significantly different evolutionary trend in the three water-sediment systems. It was most likely caused by the changes of geochemical characteristics (dissolved oxygen (DO) and nutrients). This kind of study can provide helpful information for ecological assessment and remediation strategy in eutrophic aquatic environments.

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