Antibacterial activity and phytochemical screening of some medicinal plants commonly used in Saudi Arabia against selected pathogenic microorganisms

The plant is a huge therapeutic source with enormous applications in curative industry. For new sources of antimicrobial agents, chloroform, ethyl acetate, and n–butanol extracts of two medicinal plants (Dodonaea viscose and Capparis spinosa) were prepared by liquid – liquid extraction. The plants were collected from Shuda mountain / Al-Baha region/ Saudi Arabia and then evaluated systematically. Phytochemical screening tests detect the existence of flavonoids, alkaloids, tannin, terponoids, saponnins and carbohydrates in most of the performed extracts. Antimicrobial activity was assessed against five bacterial and one fungal race. The extracts of the two plants leaves showed wide vision antibacterial activity and significant antifungal activity. The antibacterial activity (zone of inhibition) of Dodonaea viscose species varied from 30 to 18 mm, whereas of Capparis spinosa the range from 20 to 16 mm for ethyl acetate extracts, for n- butanol extracts the inhibition zone differ from 15 – 10 and 16 - 10 mm respectively. These results indicated that antimicrobial activities of plant species differ to a wide range not only between species themselves but also within the tests for antimicrobial evaluation. The current result supports the medicinal use of the leaves of these studied plants which works as an antimicrobial agent. These results compare to other studies carried out for the same plants in different countries in different environments exhibited diverse outcome in chemical constituents in the plant extracts and in their effects on tested types of micro organismswhich may have been due to a number of factors, including geographical location, season and environmental factors, and the method of extraction. This study for the two plant species was first time performed in this area of Saudi Arabia.

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A COMPARATIVE STUDY OF IN VITRO ANTIMICROBIAL ACTIVITY AND TLC STUDIES OF PETALS OF SELECTED INDIAN MEDICINAL PLANTS

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2016.v9i5.13476 ◽

2016 ◽

Vol 9 (5) ◽

pp. 259 ◽

Cited By ~ 1

Author(s):

Sumathy Rengarajan ◽

Vijayalakshmi Melanathuru ◽

Deecaraman Munuswamy ◽

Sankaranarayanan Sundaram ◽

Saravanan Thiruverkadu Selvaraj

Keyword(s):

Antimicrobial Activity ◽

Antibacterial Activity ◽

Medicinal Plants ◽

Thin Layer ◽

Thin Layer Chromatography ◽

Methanol Extract ◽

Phytochemical Screening ◽

Layer Chromatography ◽

Indian Medicinal Plants

ABSTRACTObjective: The present study was to evaluate the in vitro antibacterial activity, and thin-layer chromatography (TLC) studies from the petals of fourdifferent Indian medicinal plants (Punica granatum, Hibiscus rosa-sinensis, Cassia auriculata, and Moringa oleifera).Methods: The phytochemical screening of the methanol extract of petals of four different Indian medicinal plants was performed using standardprocedures. The antimicrobial activity was tested against various test organisms using the agar disc diffusion method.Results: The preliminary phytochemical screening for petals of four different medicinal plants revealed the presence of flavonoids, alkaloids, tannins,and saponins. From the above study, the results indicated that the methanol extract of M. oleifera petals showed the highest antimicrobial activityagainst Staphylococcus aureus and Bacillus subtilis with zone of inhibition 17.93 and 23.40, respectively, at the concentration of 20 µl/ml and alsoshowed the maximum inhibitory activity at the highest concentration (20 µl/ml) than the lowest concentration (5 µl/ml) against Gram-negativebacteria such as Escherichia coli, Proteus vulgaris, Pseudomonas aeruginosa, and Gram-positive B. subtilis and S. aureus. TLC studies of methanolextracts of petals of Indian medicinal plants revealed the presence of different phytoconstituents as evidenced by separated compounds with differentRf values.Conclusion: The results obtained in the present study indicate that the petals of four different Indian medicinal plants showed the highest antibacterialactivity and can be used as an antibacterial agent against bacterial diseases.Keywords: Phytochemicals, Antibacterial activity, Thin-layer chromatography.

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Phytochemical Screening, Antibacterial Activity and Heavy Metal Analysis of Ethnomedicinal Recipes and Their Sources Used Against Infectious Diseases

Plants ◽

10.3390/plants8110454 ◽

2019 ◽

Vol 8 (11) ◽

pp. 454 ◽

Cited By ~ 1

Author(s):

Nasir Mahmood ◽

Ruqia Nazir ◽

Muslim Khan ◽

Rashid Iqbal ◽

Muhammad Adnan ◽

...

Keyword(s):

Heavy Metal ◽

Antibacterial Activity ◽

Medicinal Plants ◽

Respiratory Tract ◽

Respiratory Tract Infections ◽

Phytochemical Screening ◽

Metal Analysis ◽

Heavy Metal Analysis ◽

Maximum Quantity ◽

Tract Infections

Plants are a rich source of secondary metabolites that have been found to have medicinal properties. The present study was conducted to evaluate the phytochemical screening, antibacterial activities and heavy metal analysis of seven medicinal plants i.e., Nigella sativa (seeds), Trigonella foenum-graecum (seeds), Brassica campestris (seeds), Pistacia integerrima (galls), Linum usitatissimum (seeds), Hyssopus officinalis (flowers), Ephedra vulgaris (dry branches) and its two recipes which are used by hakims (Practitioners of local herbal medicines), against different diseases particularly respiratory tract infections. The obtained results revealed that alkaloids (30%) and flavonoids (41%) were in maximum quantity in P. integerrima (galls) while saponins (10.9%) were in maximum quantity in Recipe 1. The antibacterial activity was determined by the agar well disc diffusion method using methanol, ethanol, chloroform and deionized water extracts. Each plant extract was tested against one Gram-positive (Streptococcus pneumonia) and two Gram-negative (Pseudomonas aeruginosa and Klebsiella pneumonia) bacteria. Maximum zones of inhibition in methanol, ethanol, chloroform and aqueous extract were seen in T. foenum-graecum against S. pneumonia (20.06 ± 0.16 mm), B. campestris against S. pneumonia (22.40 ± 0.24 mm), Recipe 2 against K. pneumonia (20.06 ± 0.16 mm) and N. sativa against S. pneumonia (20.23 ± 0.16 mm), respectively. The concentrations of heavy metals were determined by atomic absorption spectrophotometer and showed the existence of high concentration of Iron (Fe), Lead (Pb) and Chromium (Cr). Thus, it has been found that medicinal plants individually as well as their recipes are potentially active against various diseases particularly respiratory tract infections

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In Vitro Antibacterial Activity, Preliminary Phytochemical Screening Profile, and In Vivo Toxicity of Seven Traditional Medicinal Plants in Ethiopia

Traditional and Integrative Medicine ◽

10.18502/tim.v6i4.8273 ◽

2022 ◽

Author(s):

Eyerus Mekuriaw ◽

Enat Mengistu ◽

Ayana Erdedo ◽

Hassen Mamo

Keyword(s):

Antibacterial Activity ◽

Medicinal Plants ◽

Ethyl Acetate ◽

Antibacterial Properties ◽

Ethanol Extract ◽

Diffusion Method ◽

Phytochemical Screening ◽

Phytochemical Content

The threat of antibiotic-resistance calls for novel antibacterial agents. This study was aimed at screening medicinal plants for their antibacterial properties, phytochemical content and safety. Leaves of Allophylus abyssinicus (Hochst.) Radlk., Dicliptera laxata C.B.Clarke, Ligustrum vulgare L., Solanecio gigas (Vatke) c. Jeffrey and Gymnanthemum myrianthum (Hook.f.) H.Rob.; leaf and stem-bark of Olinia rochetiana A. Juss. and the seed of Cucurbita pepo L. were used. Chloroform and ethanol were used to extract G. myrianthum, D. laxata and O. rochetiana; ethyl acetate and methanol for the rest, and water for all. The extracts were tested against clinical/standard strains of Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi and Staphylococcus aureus by the agar-diffusion method. The minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) were determined. Acute toxicity to mice was checked and preliminary phytochemical screening was done. Thirteen extracts, out of 24, were active (inhibition zone >7 mm) at differing levels (9.67±0.33-25.66±0.57 mm) against at least one bacterial strain. The MICs and MBCs were 1.95-15.6 mg/mL and 7.8-125 mg/mL respectively. The aqueous extract of S. gigas, methanol extracts of L. vulgare and A. abyssinicus, and ethanol extract of O. rochetiana leaf were the most active (MIC 1.95mg/ml) against S. aureus. Ethyl acetate extracts of A. abyssinicus, L. vulgare and S. gigas; aqueous of C. pepo, O. rochetiana and G. myrianthum; and all D. laxata had no antibacterial activity. P. aeruginosa was the least susceptible to any extract, although the methanol and aqueous extracts of S. gigas performed better against it. Preliminary phytochemical screening of selected extracts for phenols, flavonoids, tannins, steroids, terpenoids, steroidal glycosides, alkaloids, saponins, resins and glycosides showed positivity at least for four of these phytochemicals with glycoside and terpenoids in nearly all extracts and resin in none. The plants were not toxic to mice at 2000 mg/kg. Further consideration of S. gigas, L. vulgare, A. abyssinicus and O. rochetiana is recommended in light of their promising potential and safety.

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Antibacterial Activity of Some Medicinal Plants in Al Baha Region, Saudi Arabia, Against Carcinogenic Bacteria Related to Gastrointestinal Cancers

Journal of Gastrointestinal Cancer ◽

10.1007/s12029-021-00793-w ◽

2022 ◽

Author(s):

Abdulrahman Ali Alzandi ◽

Essam Ahmed Taher ◽

Mohamed Azizi ◽

Nageeb A. Al-Sagheer ◽

Abdul Wali Al-Khulaidi ◽

...

Keyword(s):

Antibacterial Activity ◽

Saudi Arabia ◽

Medicinal Plants ◽

Gastrointestinal Cancers

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