Association between plasma selenium level and NRF2 target genes expression in humans

2015 ◽  
Vol 30 ◽  
pp. 102-106 ◽  
Author(s):  
Edyta Reszka ◽  
Edyta Wieczorek ◽  
Ewa Jablonska ◽  
Beata Janasik ◽  
Wojciech Fendler ◽  
...  
Keyword(s):  
Target Genes ◽  
Selenium Level ◽  
Plasma Selenium ◽  
Genes Expression

scholarly journals miR-215 Targeting Novel Genes EREG, NIPAL1 and PTPRU Regulates the Resistance to E.coli F18 in Piglets

Genes ◽  
2020 ◽  
Vol 11 (9) ◽  
pp. 1053
Author(s):  
Chao-Hui Dai ◽  
Fang Wang ◽  
Shi-Qin Wang ◽  
Zheng-Chang Wu ◽  
Sheng-Long Wu ◽  
...  
Keyword(s):  
Target Genes ◽  
Core Promoter ◽  
Negative Control ◽  
Enzyme Linked Immunosorbent Assay ◽  
Nucleotide Polymorphisms ◽  
Weaned Piglets ◽  
Promoter Regions ◽  
E Coli ◽  
Rnai Technology ◽  
Genes Expression

Previous research has revealed that miR-215 might be an important miRNA regulating weaned piglets’ resistance to Escherichia coli (E. coli) F18. In this study, target genes of miR-215 were identified by RNA-seq, bioinformatics analysis and dual luciferase detection. The relationship between target genes and E. coli infection was explored by RNAi technology, combined with E. coli stimulation and enzyme linked immunosorbent assay (ELISA) detection. Molecular regulating mechanisms of target genes expression were analyzed by methylation detection of promoter regions and dual luciferase activity assay of single nucleotide polymorphisms (SNPs) in core promoter regions. The results showed that miR-215 could target EREG, NIPAL1 and PTPRU genes. Expression levels of three genes in porcine intestinal epithelial cells (IPEC-J2) in the RNAi group were significantly lower than those in the negative control pGMLV vector (pGMLV-NC) group after E. coli F18 stimulation, while cytokines levels of TNF-α and IL-1β in the RNAi group were significantly higher than in the pGMLV-NC group. Variant sites in the promoter region of three genes could affect their promoter activities. These results suggested that miR-215 could regulate weaned piglets’ resistance to E. coli F18 by targeting EREG, NIPAL1 and PTPRU genes. This study is the first to annotate new biological functions of EREG, NIPAL1 and PTPRU genes in pigs, and provides a new experimental basis and reference for the research of piglets disease-resistance breeding.

scholarly journals P476SREBF1, SREBF2 and their target genes expression is different in wistar and SHR rats under high cholesterol treatment conditions

2014 ◽  
Vol 103 (suppl 1) ◽  
pp. S87.1-S87
Author(s):  
HV Portnichenko ◽  
SV Goncharov ◽  
LV Tumanovska ◽  
DO Stroy ◽  
OS Moibenko ◽  
...  
Keyword(s):  
Target Genes ◽  
Shr Rats ◽  
High Cholesterol ◽  
Treatment Conditions ◽  
Cholesterol Treatment ◽  
Genes Expression

Small effect on plasma selenium level by occupational lead exposure

1987 ◽  
Vol 66 ◽  
pp. 39-43 ◽  
Author(s):  
A GUSTAFSON ◽  
A SCHUTZ ◽  
P ANDERSSON ◽  
S SKERFVING
Keyword(s):  
Lead Exposure ◽  
Selenium Level ◽  
Plasma Selenium ◽  
Occupational Lead Exposure

scholarly journals Role of the Nrf2-ARE Pathway in Liver Diseases

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Sang Mi Shin ◽  
Ji Hye Yang ◽  
Sung Hwan Ki
Keyword(s):  
Liver Diseases ◽  
Target Genes ◽  
Antioxidant Response ◽  
Related Factor ◽  
Genes Expression ◽  
Wide Range ◽  
Range Of Functions ◽  
Central Organ ◽  
Essential Transcription Factor

The liver is a central organ that performs a wide range of functions such as detoxification and metabolic homeostasis. Since it is a metabolically active organ, liver is particularly susceptible to oxidative stress. It is well documented that liver diseases including hepatitis, fibrosis, cirrhosis, and hepatocellular carcinoma are highly associated with antioxidant capacity. NF-E2-related factor-2 (Nrf2) is an essential transcription factor that regulates an array of detoxifying and antioxidant defense genes expression in the liver. It is activated in response to electrophiles and induces its target genes by binding to the antioxidant response element (ARE). Therefore, the roles of the Nrf2-ARE pathway in liver diseases have been extensively investigated. Studies from several animal models suggest that the Nrf2-ARE pathway collectively exhibits diverse biological functions against viral hepatitis, alcoholic and nonalcoholic liver disease, fibrosis, and cancer via target gene expression. In this review, we will discuss the role of the Nrf2-ARE pathway in liver pathophysiology and the potential application of Nrf2 as a therapeutic target to prevent and treat liver diseases.

scholarly journals Feeding of Enterococcus faecium NCIMB 10415 Leads to Intestinal miRNA-423-5p-Induced Regulation of Immune-Relevant Genes

2016 ◽  
Vol 82 (8) ◽  
pp. 2263-2269 ◽  
Author(s):  
Susanne Kreuzer-Redmer ◽  
Jennifer C. Bekurtz ◽  
Danny Arends ◽  
Ralf Bortfeldt ◽  
Barbara Kutz-Lohroff ◽  
...  
Keyword(s):  
Mode Of Action ◽  
Enterococcus Faecium ◽  
Target Genes ◽  
Immune Cell ◽  
Animal Health ◽  
Luciferase Reporter ◽  
Content Type ◽  
Immunoglobulin Kappa ◽  
Genes Expression ◽  
Reporter Assays

ABSTRACTProbiotics are widely used in human and animal health, but little is known about the mode of action of probiotics. One possible mechanism at the molecular level could be an influence on microRNAs (miRNAs) and the related immune-relevant target genes. Here, we analyzed differential expression of miRNA and potential target genes of ileal and jejunal lymphatic tissues fromEnterococcus faeciumNCIMB 10415-fed piglets versus untreated controls by using next-generation sequencing. We identified miR-423-5p as being greatly affected by the treatment group (2.32-fold;P= 0.014). Validation by reverse transcription-quantitative PCR (RT-qPCR) confirmed a significant upregulation of miR-423-5p (2.11-fold;P= 0.03) and, additionally, downregulation of the important immune-relevant immunoglobulin lambda light C region (IGLC) (0.61-fold;P= 0.03) and immunoglobulin kappa constant (IGKC) (0.69-fold;P= 0.04) target genes. Expression analysis of miR-423-5p and IGLC at different age points shows a clear anticorrelated relationship. Luciferase reporter assays with a HeLa cell line verified IGLC as a target of miR-423-5p. The results provided evidence for an effect of feeding ofE. faeciumon the expression of miR-423-5p and on the regulation of the IGLC gene through miR-423-5p. This might be a possible mode of action ofE. faeciumon immune cell regulation in the small intestine.

scholarly journals Cryptotanshinone Reverses Cisplatin Resistance of Human Lung Carcinoma A549 Cells through Down-Regulating Nrf2 Pathway

2015 ◽  
Vol 37 (2) ◽  
pp. 816-824 ◽  
Author(s):  
Chen Xia ◽  
Xupeng Bai ◽  
Xiangyu Hou ◽  
Xiaoli Gou ◽  
Yongtao Wang ◽  
...  
Keyword(s):  
Target Genes ◽  
Salvia Miltiorrhiza ◽  
A549 Cells ◽  
Nrf2 Pathway ◽  
Expression Levels ◽  
Endogenous Expression ◽  
Sulforhodamine B ◽  
Genes Expression ◽  
Human Lung Carcinoma ◽  
Nrf2 Expression

Background/Aims: To explore whether Nrf2 was associated with drug-resistance in cisplatin resistant A549 (A549/DDP) cells, and if cryptotanshinone (CTS), one of the bioactive compounds isolated from the roots of Salvia miltiorrhiza Bunge (Danshen), could enhance the sensitivity in A549/DDP cells towards cisplatin. Methods: A549 and A549/DDP cells were subjected to various treatments, and then Sulforhodamine B (SRB) assay, flow cytometry analysis and western immunoblotting analysis were applied to determine IC50, apoptotic status and expressions of Nrf2 and its downstream genes. Results: The endogenous expression levels of Nrf2 as well as its target genes including GCLC, GCLM, HO-1, NQO1 and MRP1 were much higher in A549/DDP cells than those of A549 cells and the susceptibility of A549/DDP cells to cisplatin was partially restored by silencing Nrf2. The combination of CTS and cisplatin led to cell death and apoptosis through sensitizing A549/DDP cells towards cisplatin compared with cisplatin mono-treatment, however, this reversal role could be abolished by Nrf2 knockdown. Specifically, CTS obviously diminished Nrf2 expression, thus contributing to the decrease of Nrf2-target genes expression levels. Meanwhile, we also discovered that CTS triggered several other signals involving in chemoresistance such as MAPKs, Akt and STAT3 pathway. Conclusion: Our data indicated CTS may be developed as a potential sensitizer cooperating with anticancer drugs to combat chemoresistant carcinoma through the inhibition of the Nrf2 pathway.

scholarly journals HspB1 Overexpression Improves Lifespan and Stress Resistance in an Invertebrate Model

2021 ◽  
Author(s):  
Courtney Carroll Alexander ◽  
Erin Munkáscy ◽  
Haven Tillmon ◽  
Tamara Fraker ◽  
Jessica Scheirer ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Stress Resistance ◽  
Target Genes ◽  
Small Heat Shock Protein ◽  
Life Extension ◽  
Mole Rat ◽  
Genes Expression ◽  
Heat Response ◽  
High Level

Abstract To explore the role of the small heat shock protein beta 1 (HspB1, also known as Hsp25 in rodents and Hsp27 in humans) in longevity, we created a Caenorhabiditis elegans model with a high level of ubiquitous expression of the naked mole-rat HspB1 protein. The worms showed increased lifespan under multiple conditions and also increased resistance to heat stress. RNAi experiments suggest that HspB1-induced life extension is dependent on the transcription factors skn-1 (Nrf2) and hsf-1 (Hsf1). RNAseq from HspB1 worms showed an enrichment in several skn-1 target genes, including collagen proteins and lysosomal genes. Expression of HspB1 also improved functional outcomes regulated by SKN-1, specifically oxidative stress resistance and pharyngeal integrity. This work is the first to link a small heat shock protein with collagen function, suggesting a novel role for HspB1 as a hub between canonical heat response signaling and SKN-1 transcription.

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