Antibacterial performance and in vivo diabetic wound healing of curcumin loaded gum tragacanth/poly(ε-caprolactone) electrospun nanofibers

Nano (or) microfiber structures made from biodegradable polymers offer many benefits for biomedical applications, including the tissue engineering system. In this study, a mixture of poly (lactic acid) (PLA)/gelatin (GEL) conjugated epidermal growth factor (EGF) electrospun nanofiber scaffolds, which may have potential applications for nursing care of diabetic wound healing. The amount of EGF conjugation on the PLA/GEL nanofibers was quantitated by X-ray photoelectron spectroscopy. A morphological examination of the electrospun nanofibers was performed using scanning electron microscopy (SEM) and transmission electron microscopy (TEM), which has bead-free morphology nanofibers. After characterizing, the PLA/GEL and PLA/GEL/EGF scaffolds were selected for further investigation based on their superior mechanical properties, including tensile strength, maximum elongation, and tensile modulus. We evaluated the antibacterial activity, cell proliferation and the efficiency of diabetic wound healing in vivo. The antibacterial effect of EGF present in the PLA/GEL nanofibers was assessed using the zone inhibition test against S. aureus and E. coli. In vitro cell proliferation activity was well maintained after the PLA/GEL conjugation and was compared to that of EGF with L929 fibroblast cells. The nanofiber PLA/GEL conjugated with EGF exerted greater curative activities in vivo compared to the control groups or EGF solutions. This study showed that the nanofiber PLA/GEL in combination with EGF could potentially be used as a novel material for nursing care of wound healing by antibacterial, increasing inflammatory cell, and re-epithelialization.

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Co-Transplantation of Skin-Derived Precursors and Collagen Sponge Facilitates Diabetic Wound Healing by Promoting Local Vascular Regeneration

Cellular Physiology and Biochemistry ◽

10.1159/000438537 ◽

2015 ◽

Vol 37 (5) ◽

pp. 1725-1737 ◽

Cited By ~ 15

Author(s):

Tingyu Ke ◽

Mei Yang ◽

Duo Mao ◽

Meifeng Zhu ◽

Yongzhe Che ◽

...

Keyword(s):

Wound Healing ◽

Wound Repair ◽

Expression Patterns ◽

Collagen Sponge ◽

Health Concern ◽

Diabetic Wound ◽

Paracrine Signalling ◽

Vascular Regeneration ◽

Diabetic Wound Healing

Background/Aims: Impaired diabetes wound healing can often lead to serious complications and remains a major health concern due to the lack of effective therapeutic approaches. Compromised angiogenesis, disrupted growth factor and cytokine activity are all attributable to diabetic wound healing impairment. The skin-derived precursors (SKPs) have been shown to differentiate into vascular and nerve cells, both of which are crucial components for wound repair. Given their easy accessibility and multipotency, the SKPs were proposed as an ideal therapeutic candidate for diabetic wound healing. Since the efficacy of cell therapy is limited by poor cell survival, collagen sponge was employed for better SKPs delivery. Methods: SKPs were isolated and transplanted directly to the wound areas of diabetic mice in the absence and presence of collagen sponge. The effects of SKPs and/or collagen sponge on diabetic wound healing were examined histologically as well as immunostaining of isolectin and α-SMA. Mechanisms via which the SKPs facilitate wound healing were then investigated by transplanting SKPs that have been pre-labelled with a fluorescence dye, Dil. Expression patterns of Dil and an SKP marker, nestin, was also examined. Results and Conclusion: Accelerated wound healing and enhanced local capillary regeneration could be observed 14 days after skin ablation from both SKPs and collagen sponge co-transplanted and collagen sponge only groups. Subsequent analyses further revealed superior pro-angiogenic effects from the SKP and collagen sponge co-delivered group, which are mainly attributable to in vivo transdifferentation and paracrine signalling of the SKPs.

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Synergistic Effects of Photobiomodulation Therapy with Combined Wavelength on Diabetic Wound Healing In Vitro and In Vivo

Photobiomodulation Photomedicine and Laser Surgery ◽

10.1089/photob.2021.0068 ◽

2021 ◽

Author(s):

Weijie Cai ◽

Musha Hamushan ◽

Yubo Zhang ◽

Zhengyu Xu ◽

Zun Ren ◽

...

Keyword(s):

Wound Healing ◽

Synergistic Effects ◽

Photobiomodulation Therapy ◽

Diabetic Wound ◽

Diabetic Wound Healing

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Identification of Structurally Similar Phytochemicals to Quercetin with High SIRT1 Binding Affinity and Improving Diabetic Wound Healing by Using In silico Approaches

Biointerface Research in Applied Chemistry ◽

10.33263/briac126.76217632 ◽

2021 ◽

Vol 12 (6) ◽

pp. 7621-7632

Keyword(s):

Wound Healing ◽

In Silico ◽

Binding Free Energy ◽

Biological Properties ◽

In Vivo Studies ◽

Diabetic Wound ◽

Binding Modes ◽

Diabetic Wound Healing

Diabetes Mellitus is the most prevalent metabolic disorder that is increasing at an alarming rate worldwide. The unregulated glucose level leads to various types of health disorders, and one of the major diabetic complications is delayed wound healing. Due to the more side effects of synthetic drugs, there is a need to explore plants and their phytochemicals for medicinal purposes. It was found that Quercetin, a flavonoid, increases the rate of diabetic wound healing by enhancing the expression of SIRT1. This demands more insight towards Quercetin and its similar compounds, as it is hypothesized that similar compounds may have similar biological properties. Thus similarity searching was done to identify the most similar compounds of Quercetin, and then the molecular docking of the screened compounds was performed using AutoDock Vina. The unique ligands were docked into the active site of SIRT1 protein (PDB ID: 4ZZJ). The binding free energy of the interacting ligand with the protein was estimated. Six compounds were identified which possess the maximum structural similarity with Quercetin, and upon docking, it was found that gossypetin and herbacetin have similar binding modes and binding energy as that of Quercetin (-7.5 kcal/mol). Therefore, the hypothesis has been validated by in silico analysis. Our study identified two phytochemicals, Gossypetin, and Herbacetin which can prove beneficial for improving diabetic wound healing but needs to be validated further by in vitro and in vivo studies.

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Shedding Light on a New Treatment for Diabetic Wound Healing: A Review on Phototherapy

The Scientific World JOURNAL ◽

10.1155/2014/398412 ◽

2014 ◽

Vol 2014 ◽

pp. 1-13 ◽

Cited By ~ 39

Author(s):

Nicolette N. Houreld

Keyword(s):

Wound Healing ◽

Laser Irradiation ◽

Diabetic Wound ◽

Impaired Wound Healing ◽

Diabetic Wound Healing ◽

New Treatment ◽

Underlying Mechanisms ◽

Intensity Laser

Impaired wound healing is a common complication associated with diabetes with complex pathophysiological underlying mechanisms and often necessitates amputation. With the advancement in laser technology, irradiation of these wounds with low-intensity laser irradiation (LILI) or phototherapy, has shown a vast improvement in wound healing. At the correct laser parameters, LILI has shown to increase migration, viability, and proliferation of diabetic cellsin vitro; there is a stimulatory effect on the mitochondria with a resulting increase in adenosine triphosphate (ATP). In addition, LILI also has an anti-inflammatory and protective effect on these cells. In light of the ever present threat of diabetic foot ulcers, infection, and amputation, new improved therapies and the fortification of wound healing research deserves better prioritization. In this review we look at the complications associated with diabetic wound healing and the effect of laser irradiation bothin vitroandin vivoin diabetic wound healing.

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Strain-Programmable Patch for Diabetic Wound Healing

10.1101/2021.06.07.447423 ◽

2021 ◽

Author(s):

Georgios Theocharidis ◽

Hyunwoo Yuk ◽

Heejung Roh ◽

Liu Wang ◽

Ikram Mezghani ◽

...

Keyword(s):

Wound Healing ◽

Chronic Wounds ◽

Ex Vivo ◽

Numerical Models ◽

Diabetic Patients ◽

Diabetic Wound ◽

Memory Mechanism ◽

Culture Models ◽

Diabetic Wound Healing

Chronic wounds with impaired healing capability such as diabetic foot ulcers (DFU) are devastating complications in diabetic patients, inflicting rapidly growing clinical and economic burdens in aging societies. Despite recent advances in therapeutic approaches, limited benefits of the existing solutions highlight the critical need for novel therapeutic solutions for diabetic wound healing. Here we propose a strain-programmable patch capable of rapid robust adhesion on and programmable mechanical contraction of wet wounded tissues over days to offer a new therapeutic platform for diabetic wounds. The strain-programmable patch, consisting of a dried bioadhesive layer and a pre-stretched elastomer backing, implements a hydration-based shape-memory mechanism to achieve both uniaxial and biaxial contractions and stress remodeling of wet wounds in a programmable manner. We develop theoretical and numerical models to rationally guide the strain-programming and mechanical modulation of wounds. In vivo rodent and ex vivo human skin culture models validate the programmability and efficacy of the proposed platform and identify mechanisms of action for accelerated diabetic wound healing.

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Simvastatin nanoparticles loaded polymeric film as a potential strategy for diabetic wound healing: in vitro and in vivo evaluation

Current Drug Delivery ◽

10.2174/1567201818666210720150929 ◽

2021 ◽

Vol 18 ◽

Author(s):

Saima Tufail ◽

Muhammad Irfan Siddique ◽

Muhammad Sarfraz ◽

Muhammad Farhan Sohail ◽

Muhammad Nabeel Shahid ◽

...

Keyword(s):

Wound Healing ◽

Healing Process ◽

Chitosan Nanoparticles ◽

Albino Rats ◽

Wound Healing Process ◽

Diabetic Wound ◽

In Vivo Evaluation ◽

Diabetic Wound Healing

Introduction: The pleiotropic effects of statins are recently explored for wound healing through angiogenesis and lymph-angiogenesis that could be of great importance in diabetic wounds. Aim: Aim of the present study is to fabricate nanofilm embedded with simvastatin loaded chitosan nanoparticles (CS-SIM-NPs) has been reported herein to explore the efficacy of SIM in diabetic wound healing. Methods: The NPs, prepared via ionic gelation, were 173nm ± 2.645 in size with a zeta potential -0.299 ± 0.009 and PDI 0.051 ± 0.088 with excellent encapsulation efficiency (99.97%). The optimized formulation (CS: TPP, 1:1) that exhibited the highest drug release (91.64%) was incorporated into polymeric nanofilm (HPMC, Sodium alginate, PVA), followed by in vitro characterization. The optimized nanofilm was applied to the wound created on the back of diabetes-induced (with alloxan injection 120 mg/kg) albino rats. Results: The results showed significant (p < 0.05) improvement in the wound healing process compared to the diabetes-induced non-treated group. The results highlighted the importance of nanofilms loaded with SIM-NPs in diabetic wound healing through angiogenesis promotion at the wound site. Conclusion: Thus, CS-SIM-NPs loaded polymeric nanofilms could be an emerging diabetic wound healing agent in the industry of nanomedicines.

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Nanofiber/hydrogel Core-shell Scaffolds With Three-dimensional Multilayer Patterned Structure for Accelerating Diabetic Wound Healing

10.21203/rs.3.rs-952440/v1 ◽

2021 ◽

Author(s):

Jiankai Li ◽

Tianshuai Zhang ◽

Mingmang Pan ◽

Feng Xue ◽

Fang Lv ◽

...

Keyword(s):

Wound Healing ◽

Three Dimensional ◽

Vapor Permeability ◽

Core Shell ◽

Diabetic Wound ◽

3D Network ◽

Diabetic Wound Healing ◽

Diabetic Wounds

Abstract Impaired angiogenesis is one of the predominant reasons for non-healing diabetic wounds. Herein, a nanofiber/ hydrogel core-shell scaffold with three-dimensional (3D) multilayer patterned structure (3D-PT-P/GM) was introduced for promoting diabetic wound healing with improved angiogenesis. The results showed that the 3D-PT-P/GM scaffolds possessed multilayered structure with interlayer spacing of about 15-80 μm, and the hexagonal micropatterned structures were uniformly distributed on the surface of each layer. The nanofibers in the scaffold exhibited distinct core-shell structures with Gelatin methacryloyl (GelMA) hydrogel as the shell and Poly (D, L-lactic acid) (PDLLA) as the core. The results showed that the porosity, water retention time and water vapor permeability of the 3D-PT-P/GM scaffolds increased to 1.6 times, 21 times, and 1.9 times than that of the two-dimensional (2D) PDLLA nanofibrous scaffolds, respectively. The in vitro studies showed that the 3D-PT-P/GM scaffolds could significantly promote cell adhesion, proliferation, infiltration and migration throughout the scaffolds, and the expression of cellular communication protein-related genes, as well as angiogenesis-related genes in the same group, was remarkably upregulated. The in vivo results further demonstrated that the 3D-PT-P/GM scaffolds could not only effectively absorb exudate and provide a moist environment for the wound sites, but also significantly promote the formation of a 3D network of capillaries. As a result, the healing of diabetic wounds was accelerated with enhanced angiogenesis, granulation tissue formation, and collagen deposition. These results indicate that nanofiber/ hydrogel core-shell scaffolds with 3D multilayer patterned structures could provide a new strategy for facilitating chronic wound healing.

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Nanotechnology-based Therapeutic Applications: In Vitro, In Vivo Clinical Studies for Diabetic Wound Healing

Biomaterials Science ◽

10.1039/d1bm01211h ◽

2021 ◽

Author(s):

Sheikh Tanzina Haque ◽

Subbroto Kumar Saha ◽

Md. Enamul Haque ◽

Nirupam Biswas

Keyword(s):

Wound Healing ◽

Side Effects ◽

Conventional Treatment ◽

Treatment Modalities ◽

Diabetic Wound ◽

Chronic Complications ◽

Diabetic Wound Healing ◽

Diabetic Wounds

Diabetic wounds often presage chronic complications that are difficult to treat. Unfortunately, existing conventional treatment modalities often warrant unpremeditated side effects, given the need to develop alternative therapeutic phenotypes that...

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A Synthetic Curcuminoid Analog, (2E,6E)-2,6-bis(2-(trifluoromethyl)benzylidene)cyclohexanone, Ameliorates Impaired Wound Healing in Streptozotocin-Induced Diabetic Mice by Increasing miR-146a

Molecules ◽

10.3390/molecules25040920 ◽

2020 ◽

Vol 25 (4) ◽

pp. 920

Author(s):

Jingjuan Huang ◽

Jia Fu ◽

Bing Liu ◽

Rui Wang ◽

Tianhui You

Keyword(s):

Wound Healing ◽

Umbilical Vein ◽

Interleukin 1 ◽

Diabetic Wound ◽

Diabetic Mice ◽

Skin Wound Healing ◽

Promising Alternative ◽

Impaired Wound Healing ◽

Diabetic Wound Healing

The impairment in diabetic wound healing represents a significant clinical problem, with no efficient targeted treatments for these wound disorders. Curcumin is well confirmed to improve diabetic wound healing, however, its low bioavailability and poor solubility severely limit its clinical application. This study aims to provide the pharmacological basis for the use of (2E,6E)-2,6-bis(2-(trifluoromethyl)benzylidene)cyclohexanone (C66). The results showed that topically applied C66 improved cutaneous wound healing in vivo. Further studies showed that C66 treatment increased the level of microRNA-146a (miR-146a) in the wounds in streptozotocin (STZ)-induced diabetic mice, downregulated the expression of interleukin-1 receptor-associated kinase 1 (IRAK1) and phosphorylated nuclear factor-κB (NF-κB) p65 subunit (p-p65) (both p < 0.05), and suppressed the mRNA expression of inflammation-related cytokines, tumor necrosis factor-α (TNF-α), interleukin-8 (IL-8), and interleukin-6 (IL-6). The in vitro data obtained in human umbilical vein endothelial cells (HUVECs) showed that C66 could reverse high glucose (HG)-induced NF-κB activation due to upregulation of miR-146a expression, which matched the in vivo findings. In conclusion, the present study indicates that C66 exerts anti-inflammation activity and accelerates skin wound healing of diabetic mice, probably via increasing miR-146a and inhibiting the NF-κB-mediated inflammation pathway. Therefore, C66 may be a promising alternative for the treatment of diabetic wounds.

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