A tough synthetic hydrogel with excellent post-loading of drugs for promoting the healing of infected wounds in vivo

2021 ◽  
pp. 112577
Author(s):  
Liwen Deng ◽  
Huidan Lu ◽  
Chenxi Tu ◽  
Tong Zhou ◽  
Wangbei Cao ◽  
...  
Keyword(s):  
Synthetic Hydrogel ◽  
Infected Wounds

Preparation of biocompatible wound dressings with dual release of antibiotic and platelet-rich plasma for enhancing infected wound healing

2021 ◽  
pp. 088532822199601
Author(s):  
Linying Shi ◽  
Fang Lin ◽  
Mou Zhou ◽  
Yanhui Li ◽  
Wendan Li ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wound Dressing ◽  
Platelet Rich Plasma ◽  
Healing Process ◽  
Wound Dressings ◽  
Inflammatory Factors ◽  
Gelatin Microspheres ◽  
Infected Wounds ◽  
Dual Release

The ever-growing threats of bacterial infection and chronic wound healing have provoked an urgent need for novel antibacterial wound dressings. In this study, we developed a wound dressing for the treatment of infected wounds, which can reduce the inflammatory period (through the use of gentamycin sulfate (GS)) and enhance the granulation stage (through the addition of platelet-rich plasma (PRP)). Herein, the sustained antimicrobial CMC/GMs@GS/PRP wound dressings were developed by using gelatin microspheres (GMs) loading GS and PRP, covalent bonding to carboxymethyl chitosan (CMC). The prepared dressings exhibited high water uptake capability, appropriate porosity, excellent mechanical properties, sustain release of PRP and GS. Meanwhile, the wound dressing showed good biocompatibility and excellent antibacterial ability against Gram-negative and Gram-positive bacteria. Moreover, in vivo experiments further demonstrated that the prepared dressings could accelerate the healing process of E. coli and S. aureus-infected full-thickness wounds i n vivo, reepithelialization, collagen deposition and angiogenesis. In addition, the treatment of CMC/GMs@GS/PRP wound dressing could reduce bacterial count, inhibit pro-inflammatory factors (TNF-α, IL-1β and IL-6), and enhance anti-inflammatory factors (TGF-β1). The findings of this study suggested that biocompatible wound dressings with dual release of GS and PRP have great potential in the treatment of chronic and infected wounds.

Can infected wounds be decontaminated with the use of the CO 2 laser: An in vivo comparative study

2003 ◽  
Author(s):  
Jose Z. Lima Verde Santos ◽  
Antonio L. Barbosa Pinheiro ◽  
Jerlucia Cavalcanti das Neves ◽  
Kesia X. d. F. Ribeiro de Sena ◽  
Marcos A. Matos de Oliveira
Keyword(s):  
Comparative Study ◽  
Infected Wounds

scholarly journals Polyethylene Glycol-Based Synthetic Hydrogel Sealant for Closing Vitrectomy Wounds: An In Vivo and Histological Study

2016 ◽  
Vol 5 (3) ◽  
pp. 7 ◽  
Author(s):  
Sujin Hoshi ◽  
Fumiki Okamoto ◽  
Mikki Arai ◽  
Tatsuo Hirose ◽  
Shinichi Fukuda ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Histological Study ◽  
Synthetic Hydrogel

scholarly journals Eradication of Mature Bacterial Biofilms with Concurrent Improvement in Chronic Wound Healing Using Silver Nanoparticle Hydrogel Treatment

Biomedicines ◽  
2021 ◽  
Vol 9 (9) ◽  
pp. 1182
Author(s):  
Hanif Haidari ◽  
Richard Bright ◽  
Sanjay Garg ◽  
Krasimir Vasilev ◽  
Allison J. Cowin ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wound Repair ◽  
Wound Closure ◽  
In Vivo Studies ◽  
Release Mechanism ◽  
Impaired Wound Healing ◽  
Biofilm Model ◽  
Infected Wounds ◽  
Delivery Platform

Biofilm-associated infections are a major cause of impaired wound healing. Despite the broad spectrum of anti-bacterial benefits provided by silver nanoparticles (AgNPs), these materials still cause controversy due to cytotoxicity and a lack of efficacy against mature biofilms. Herein, highly potent ultrasmall AgNPs were combined with a biocompatible hydrogel with integrated synergistic functionalities to facilitate elimination of clinically relevant mature biofilms in-vivo combined with improved wound healing capacity. The delivery platform showed a superior release mechanism, reflected by high biocompatibility, hemocompatibility, and extended antibacterial efficacy. In vivo studies using the S. aureus wound biofilm model showed that the AgNP hydrogel (200 µg/g) was highly effective in eliminating biofilm infection and promoting wound repair compared to the controls, including silver sulfadiazine (Ag SD). Treatment of infected wounds with the AgNP hydrogel resulted in faster wound closure (46% closure compared to 20% for Ag SD) and accelerated wound re-epithelization (60% for AgNP), as well as improved early collagen deposition. The AgNP hydrogel did not show any toxicity to tissue and/or organs. These findings suggest that the developed AgNP hydrogel has the potential to be a safe wound treatment capable of eliminating infection and providing a safe yet effective strategy for the treatment of infected wounds.

scholarly journals IN VIVO EFFICACY OF TOPICAL BINAHONG (ANREDERA CORDIFOLIA (TEN.) STEENIS) LEAF ETHANOLIC EXTRACT ON TRANSFORMING GROWTH FACTOR-BETA 1 IN INFECTED WOUNDS

2021 ◽  
pp. 99-102
Author(s):  
RIZKI ANDINI NAWAWI ◽  
MUHAMMAD TOTONG KAMALUDDIN ◽  
THEODORUS
Keyword(s):  
Growth Factor ◽  
Transforming Growth Factor Beta ◽  
Transforming Growth Factor ◽  
Ethanolic Extract ◽  
Bacterial Count ◽  
Treatment Groups ◽  
Infected Wounds ◽  
Growth Factor Beta ◽  
Tgf Β1

Objective: This study’s aim was to assess the efficacy of topical Binahong (Anredera cordifolia (Ten.) Steenis) leaf ethanolic extract administration on serum transforming growth factor-beta 1 (TGF-β1) in infected wounds. Methods: An experimental study, in vivo, was conducted in the Biotechnology Laboratory and Animal House, Faculty of Medicine, Universitas Sriwijaya, Palembang, from July to September 2020. There were 30 male Wistar rats aged 10–12 weeks with excisional wounds infected with Staphylococcus aureus ATCC 25923. The rats were divided into five groups and received three concentrations of Binahong leaf extracts (2.5%, 5%, and 10%), salve base, and povidone iodine 10% topically twice daily for 14 days. Serum was obtained before treatment and after 14 days of treatment. Wound area and bacterial count were also recorded and analyzed. Data analysis was performed using computer software. Results: Wound size and bacterial count were significantly decreased in treatment groups receiving topical Binahong leaf ethanolic extract. No significant increase in serum TGF-β1 was observed in all treatment groups. Conclusion: Topical administration of Binahong leaf ethanolic extract on rats with infected wounds for 14 days did not significantly increase serum TGF-β1.

Hydrogel Strategy to Augment Tissue Adenosine to Improve Hindlimb Perfusion

2021 ◽  
Author(s):  
Michael N. Sayegh ◽  
Kimberly A. Cooney ◽  
Woojin M. Han ◽  
Lanfang Wang ◽  
Frederick Strobel ◽  
...  
Keyword(s):  
Ethylene Glycol ◽  
Doppler Imaging ◽  
Rheological Parameters ◽  
Poly Ethylene Glycol ◽  
Antithrombotic Agent ◽  
Synthetic Hydrogel ◽  
Poly Ethylene ◽  
Peripheral Arterial

Objective: Adenosine is an important vasodilatory, anti-inflammatory, and antithrombotic agent; however, its delivery for the treatment of cardiovascular diseases is challenging due to the drug’s short half-life and dose-limiting side effects. Peripheral artery disease, one of the most prevalent atherosclerotic diseases of the cardiovascular system, remains without adequate nonsurgical treatments, resulting in significant morbidity due to ischemia and inflammation. Here, we hypothesize that we can use an enzyme-loaded synthetic hydrogel for local adenosine production. Approach and Results: We engineer a protease-sensitive poly(ethylene glycol)-maleimide-based hydrogel and characterize its rheological parameters when modulating poly(ethylene glycol) density and tethering a peptide to the gel backbone, then test degradation in response to collagenase. We load the gel with an ecto-nucleotidase, CD73, which catalyzes adenosine production from phosphorylated substrates, and use the CD73-loaded gel to generate adenosine in vitro and inhibit neutrophils’ oxidative burst. When delivered in vivo, the CD73 hydrogel augments adenosine levels in hindlimb skeletal muscles 24 hours after induction of peripheral arterial ischemia and increases lower limb perfusion compared with control gel in healthy mice on laser Doppler imaging. Conclusions: This enzyme-delivering hydrogel provides a strategy for local and sustained adenosine generation to improve perfusion, and future work will optimize its use for disease modulation.

scholarly journals Selective antimicrobial activity of maggots against pathogenic bacteria

2008 ◽  
Vol 57 (5) ◽  
pp. 617-625 ◽  
Author(s):  
Domen Jaklič ◽  
Aleš Lapanje ◽  
Klemen Zupančič ◽  
Dragica Smrke ◽  
Nina Gunde-Cimerman
Keyword(s):  
Pathogenic Bacteria ◽  
Chronic Wounds ◽  
Antibacterial Activities ◽  
Bacterial Strains ◽  
Infected Wounds ◽  
Before And After ◽  
Treatment Of Wounds ◽  
Maggot Therapy

Maggot therapy, also known as biosurgery, is an ancient method for the healing of chronic infected wounds. Although clinicians have reported on the beneficial activities of the Lucilia sericata larvae that have been used for healing chronic wounds, the selectivity of this therapy against the different pathogenic micro-organisms that are found in chronic wounds has never been analysed. In the present study, we have investigated the in vitro activities of larval excreta/secreta both against selected bacterial strains that frequently occur in chronically infected wounds, and against bacteria isolated directly from the larvae and their excreta/secreta. Additionally, the antibacterial activities were investigated in in vivo studies, by comparing bacterial diversity in wounds before and after the application of L. sericata larvae. In conclusion, larval therapy is highly recommended, particularly for the treatment of wounds infected with Gram-positive bacteria, like Staphylococcus aureus, but less so for wounds infected with Gram-negative bacteria, especially Proteus spp. and Pseudomonas spp. strains. Bacteria from the genus Vagococcus were resistant to the maggot excreta/secreta.

scholarly journals A PEG-Based Hydrogel for Effective Wound Care Management

2018 ◽  
Vol 27 (2) ◽  
pp. 275-284 ◽  
Author(s):  
Sen-Lu Chen ◽  
Ru-Huei Fu ◽  
Shih-Fei Liao ◽  
Shih-Ping Liu ◽  
Shinn-Zong Lin ◽  
...  
Keyword(s):  
Wound Closure ◽  
Soft Tissues ◽  
Wound Care ◽  
In Vitro Biocompatibility ◽  
Synthetic Hydrogel ◽  
Strong Adhesion ◽  
In Vivo Effects ◽  
Sealing Materials

It is extremely challenging to achieve strong adhesion in soft tissues while minimizing toxicity, tissue damage, and other side effects caused by wound sealing materials. In this study, flexible synthetic hydrogel sealants were prepared based on polyethylene glycol (PEG) materials. PEG is a synthetic material that is nontoxic and inert and, thus, suitable for use in medical products. We evaluated the in vitro biocompatibility tests of the dressings to assess cytotoxicity and irritation, sensitization, pyrogen toxicity, and systemic toxicity following the International Organization for Standardization 10993 standards and the in vivo effects of the hydrogel samples using Coloskin liquid bandages as control samples for potential in wound closure.

scholarly journals Effects of low intensity laser in in vitro bacterial culture and in vivo infected wounds

2014 ◽  
Vol 41 (1) ◽  
pp. 49-55 ◽  
Author(s):  
Pâmela Rosa Pereira ◽  
Josue Bruginski De Paula ◽  
Juliette Cielinski ◽  
Marcelo Pilonetto ◽  
Luiz Carlos Von Bahten
Keyword(s):  
Laser Therapy ◽  
Control Group ◽  
Type I ◽  
Low Intensity ◽  
Infected Wounds ◽  
Male Wistar Rats ◽  
Low Intensity Laser Therapy ◽  
Intensity Laser

OBJECTIVE: to compare the effects of low intensity laser therapy on in vitro bacterial growth and in vivo in infected wounds, and to analyze the effectiveness of the AsGa Laser technology in in vivo wound infections. METHODS: in vitro: Staphylococcus aureus were incubated on blood agar plates, half of them being irradiated with 904 nm wavelength laser and dose of 3J/cm2 daily for seven days. In vivo: 32 male Wistar rats were divided into control group (uninfected) and Experimental Group (Infected). Half of the animals had their wounds irradiated. RESULTS: in vitro: there was no statistically significant variation between the experimental groups as for the source plates and the derived ones (p>0.05). In vivo: there was a significant increase in the deposition of type I and III collagen in the wounds of the infected and irradiated animals when assessed on the fourth day of the experiment (p=0.034). CONCLUSION: low-intensity Laser Therapy applied with a wavelength of 904nm and dose 3J/cm2 did not alter the in vitro growth of S. aureus in experimental groups; in vivo, however, it showed significant increase in the deposition of type I and III collagen in the wound of infected and irradiated animals on the fourth day of the experiment.

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