scholarly journals Cryopreserved cell-laden alginate microgel bioink for 3D bioprinting of living tissues

2019 ◽  
Vol 12 ◽  
pp. 61-70 ◽  
Author(s):  
O. Jeon ◽  
Y.B. Lee ◽  
T.J. Hinton ◽  
A.W. Feinberg ◽  
E. Alsberg
Keyword(s):  
3D Bioprinting ◽  
Cryopreserved Cell ◽  
Living Tissues

scholarly journals 3D Bioprinting Strategies, Challenges, and Opportunities to Model the Lung Tissue Microenvironment and Its Function

2021 ◽  
Vol 9 ◽  
Author(s):  
Mabel Barreiro Carpio ◽  
Mohammadhossein Dabaghi ◽  
Julia Ungureanu ◽  
Martin R. Kolb ◽  
Jeremy A. Hirota ◽  
...  
Keyword(s):  
Lung Tissue ◽  
3D Bioprinting ◽  
Culture Methods ◽  
Cell Dynamics ◽  
Disease Evolution ◽  
Lung Structure ◽  
Challenges And Opportunities ◽  
Living Tissues ◽  
And Function

Human lungs are organs with an intricate hierarchical structure and complex composition; lungs also present heterogeneous mechanical properties that impose dynamic stress on different tissue components during the process of breathing. These physiological characteristics combined create a system that is challenging to model in vitro. Many efforts have been dedicated to develop reliable models that afford a better understanding of the structure of the lung and to study cell dynamics, disease evolution, and drug pharmacodynamics and pharmacokinetics in the lung. This review presents methodologies used to develop lung tissue models, highlighting their advantages and current limitations, focusing on 3D bioprinting as a promising set of technologies that can address current challenges. 3D bioprinting can be used to create 3D structures that are key to bridging the gap between current cell culture methods and living tissues. Thus, 3D bioprinting can produce lung tissue biomimetics that can be used to develop in vitro models and could eventually produce functional tissue for transplantation. Yet, printing functional synthetic tissues that recreate lung structure and function is still beyond the current capabilities of 3D bioprinting technology. Here, the current state of 3D bioprinting is described with a focus on key strategies that can be used to exploit the potential that this technology has to offer. Despite today’s limitations, results show that 3D bioprinting has unexplored potential that may be accessible by optimizing bioink composition and looking at the printing process through a holistic and creative lens.

scholarly journals Organ Bioprinting: The Next Generation Revolution in Medical Science

2020 ◽  
Author(s):  
Mahnoor Patel
Keyword(s):  
Medical Technology ◽  
Drug Testing ◽  
Medical Science ◽  
Cell Types ◽  
Organ Shortage ◽  
3D Bioprinting ◽  
Organ Donations ◽  
Organ Printing ◽  
Human Participants ◽  
Living Tissues

The idea about lab grown organs is possibly the end of drug testing on the experimental animals or the human participants. Solution of organ shortage and the desperate ending state of organ donations worldwide can be solved. 3D Bioprinting is a revolutionary mind blowing medical technology emerged in the last few years. It involves the creation of living tissues, like bones, blood vessels, heart or skin with the help of additive manufacturing which is also known as 3D Bioprinting. Unlike other printing technology for the objects, Bioprinting not only needs living cells, they also need environment for nurturing to stay them alive, like food, water and oxygen. Nowadays, these kinds of conditions are provided by microgel, such as gelatin enriched with proteins, vitamins and many other compounds for life sustaining. Furthermore, for creating the fostering conditions and fastest efficient cell growth, scientist plant cells around 3D scaffolds which made of biodegradable polymers or collagen so that organ can able to grow in fully functional living tissue. Bioprinting is time-consuming and difficult also, but by doing proper research all problems can be solved for making organs available in transplantation process. Mass production of the organs for medical purpose likely to solve in the coming next decade. Also it is too much difficult to print the complex organs. Also if the technology available more easily, tissue engineering will become more feasible than entire organ printing. Bionic ear, synthetic skin, bladder or cornea might be the first tissues to be bio printed or completely grown in the lab on demand. These tissues having small numbers of cell types, it can be the first one for fully grown bio printed organs. After this success, bio printing of more complex organs can be done in future.

Law Issues and 3D Printing

2017 ◽  
pp. 69-77
Keyword(s):  
3D Printing ◽  
Human Body ◽  
Special Interest ◽  
Emerging Technology ◽  
Body Parts ◽  
Patent Office ◽  
3D Bioprinting ◽  
Living Tissues ◽  
Near Future ◽  
The U.S

Revolution in 3D bioprinting advancing so quickly. Our special interest is focused on 3D bio printing, the printing of mammalian or human body parts. Very close to this term is cloneprint. The 3D printing living tissues is real and may be widely available in the near future. This emerging technology has generated controversies about its regulation. Another equally important issue is whether bioprinting is patentable. The U.S. Patent and Trademark Office (Patent Office) has already granted some bioprinting patents and many more applications that pending on a patent. This chapter highlighting these issues that can be part of our future.

scholarly journals Advances on Bone Substitutes through 3D Bioprinting

2020 ◽  
Vol 21 (19) ◽  
pp. 7012 ◽  
Author(s):  
Tullio Genova ◽  
Ilaria Roato ◽  
Massimo Carossa ◽  
Chiara Motta ◽  
Davide Cavagnetto ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
New Technologies ◽  
Treatment Options ◽  
Three Dimensional ◽  
Bone Substitutes ◽  
Biological Properties ◽  
3D Bioprinting ◽  
Cell Sources ◽  
Biological Cost ◽  
Living Tissues

Reconstruction of bony defects is challenging when conventional grafting methods are used because of their intrinsic limitations (biological cost and/or biological properties). Bone regeneration techniques are rapidly evolving since the introduction of three-dimensional (3D) bioprinting. Bone tissue engineering is a branch of regenerative medicine that aims to find new solutions to treat bone defects, which can be repaired by 3D printed living tissues. Its aim is to overcome the limitations of conventional treatment options by improving osteoinduction and osteoconduction. Several techniques of bone bioprinting have been developed: inkjet, extrusion, and light-based 3D printers are nowadays available. Bioinks, i.e., the printing materials, also presented an evolution over the years. It seems that these new technologies might be extremely promising for bone regeneration. The purpose of the present review is to give a comprehensive summary of the past, the present, and future developments of bone bioprinting and bioinks, focusing the attention on crucial aspects of bone bioprinting such as selecting cell sources and attaining a viable vascularization within the newly printed bone. The main bioprinters currently available on the market and their characteristics have been taken into consideration, as well.

scholarly journals Progress of 3D Bioprinting in Organ Manufacturing

Polymers ◽  
2021 ◽  
Vol 13 (18) ◽  
pp. 3178
Author(s):  
Dabin Song ◽  
Yukun Xu ◽  
Siyu Liu ◽  
Liang Wen ◽  
Xiaohong Wang
Keyword(s):  
Computer Aided Design ◽  
Three Dimensional ◽  
Research Progress ◽  
Layer By Layer ◽  
3D Bioprinting ◽  
Bioartificial Organs ◽  
Computer Aided ◽  
Bioactive Agents ◽  
Living Tissues ◽  
Aided Design

Three-dimensional (3D) bioprinting is a family of rapid prototyping technologies, which assemble biomaterials, including cells and bioactive agents, under the control of a computer-aided design model in a layer-by-layer fashion. It has great potential in organ manufacturing areas with the combination of biology, polymers, chemistry, engineering, medicine, and mechanics. At present, 3D bioprinting technologies can be used to successfully print living tissues and organs, including blood vessels, skin, bones, cartilage, kidney, heart, and liver. The unique advantages of 3D bioprinting technologies for organ manufacturing have improved the traditional medical level significantly. In this article, we summarize the latest research progress of polymers in bioartificial organ 3D printing areas. The important characteristics of the printable polymers and the typical 3D bioprinting technologies for several complex bioartificial organs, such as the heart, liver, nerve, and skin, are introduced.

scholarly journals GELMA BASED 3D BIOPRINTING IN REGENERATIVE MEDICINE

2021 ◽  
Vol 10 (3) ◽  
Author(s):  
Subhajit Hazra ◽  
Dhakshanya Predheepan ◽  
Jeba Samuel C S ◽  
GovindanT. V ◽  
Ripudaman Singh
Keyword(s):  
Stem Cells ◽  
3D Printing ◽  
Regenerative Medicine ◽  
Organ Transplantation ◽  
Human Cells ◽  
3D Bioprinting ◽  
The Past ◽  
Significant Interest ◽  
Living Tissues

Over the past few years, research and progress in 3D printing have become evident. The process of bioprinting involves the use of a bioink composed of human cells or tissue. For example, 3D printing in organ transplantation aims to develop an organ that can synchronize with other physiologic components. In the past ten years, bioprinting has made a substantial leap. It has been used in the fabrication of living tissues for its application in various areas. Moreover, this technology has also been commercialized, resulting in its significant interest from the research fraternity. Thus, this review provides a brief on the development of the field from its foundation to the current commercialization with respect to the polymer Gelatin Methacrylate. Keywords: 3D Bioprinting, Stem cells, GelMA, Regenerative Medicine

Self-Contained 3D Bioprinter for Cardiovascular and Cancer Research

2019 ◽  
Author(s):  
Prabhuti Kharel ◽  
Likitha Somasekhar ◽  
Kevin Fernando ◽  
Kunal Mitra
Keyword(s):  
Cancer Research ◽  
Structural Integrity ◽  
Environmental Parameters ◽  
Primary Objective ◽  
3D Bioprinting ◽  
Deposition Method ◽  
Fabrication Technology ◽  
Tissue Constructs ◽  
Living Tissues ◽  
Selection Of

Bioprinting is a 3D fabrication technology used to accurately dispense cell-laden biomaterials for the fabrication of complex 3D functional living tissues. A syringe-based extrusion (SBE) deposition method comprising of multiple nozzles is integrated into the system. This allows for a wider selection of biomaterials that can be used for the formation of the extracellular matrix (ECM). The 3D bioprinting system presented in this paper aims to facilitate the process of 3D bioprinting through its ability to control the environmental parameters within an enclosed printing chamber. The primary objective of this research is to print viable 3D tissue constructs seeded with cells with high structural integrity and high resolution.

scholarly journals 3D bioprinting: current status and trends—a guide to the literature and industrial practice

2021 ◽  
Author(s):  
Silvia Santoni ◽  
Simone G. Gugliandolo ◽  
Mattia Sponchioni ◽  
Davide Moscatelli ◽  
Bianca M. Colosimo
Keyword(s):  
Additive Manufacturing ◽  
Three Dimensional ◽  
Biological Tissues ◽  
Research Field ◽  
Current Status ◽  
Scientometric Analysis ◽  
3D Bioprinting ◽  
Industrial Landscape ◽  
Emerging Trends ◽  
Living Tissues

AbstractThe multidisciplinary research field of bioprinting combines additive manufacturing, biology and material sciences to create bioconstructs with three-dimensional architectures mimicking natural living tissues. The high interest in the possibility of reproducing biological tissues and organs is further boosted by the ever-increasing need for personalized medicine, thus allowing bioprinting to establish itself in the field of biomedical research, and attracting extensive research efforts from companies, universities, and research institutes alike. In this context, this paper proposes a scientometric analysis and critical review of the current literature and the industrial landscape of bioprinting to provide a clear overview of its fast-changing and complex position. The scientific literature and patenting results for 2000–2020 are reviewed and critically analyzed by retrieving 9314 scientific papers and 309 international patents in order to draw a picture of the scientific and industrial landscape in terms of top research countries, institutions, journals, authors and topics, and identifying the technology hubs worldwide. This review paper thus offers a guide to researchers interested in this field or to those who simply want to understand the emerging trends in additive manufacturing and 3D bioprinting. Graphic abstract

Low-voltage field-emission SEM of polymeric membranes for glucose biosensors

1990 ◽  
Vol 48 (3) ◽  
pp. 860-861
Author(s):  
Lorna K. Mayo ◽  
Kenneth C. Moore ◽  
Mark A. Arnold
Keyword(s):  
Glucose Sensor ◽  
Low Voltage ◽  
Glucose Sensors ◽  
Polymeric Membranes ◽  
Artificial Endocrine Pancreas ◽  
Self Monitoring ◽  
Conducting Materials ◽  
Insulin Dependent ◽  
Living Tissues ◽  
Voltage Scanning

An implantable artificial endocrine pancreas consisting of a glucose sensor and a closed-loop insulin delivery system could potentially replace the need for glucose self-monitoring and regulation among insulin dependent diabetics. Achieving such a break through largely depends on the development of an appropriate, biocompatible membrane for the sensor. Biocompatibility is crucial since changes in the glucose sensors membrane resulting from attack by orinter action with living tissues can interfere with sensor reliability and accuracy. If such interactions can be understood, however, compensations can be made for their effects. Current polymer technology offers several possible membranes that meet the unique chemical dynamics required of a glucose sensor. Two of the most promising polymer membranes are polytetrafluoroethylene (PTFE) and silicone (Si). Low-voltage scanning electron microscopy, which is an excellent technique for characterizing a variety of polymeric and non-conducting materials, 27 was applied to the examination of experimental sensor membranes.

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