Phenolic profile, enzyme inhibition and antioxidant activities and bioinformatics analysis of leaf and stem bark of Ficus sycomorus L.

This study revealed polyphenolic content, nutritive content, antioxidant activity, and phenolic profile of methanol and aqueous extracts ofPunica granatumpeel extract. For this, extracts were screened for possible antioxidant activities by free radical scavenging activity (DPPH), hydrogen peroxide scavenging activity and ferric-reducing antioxidant power (FRAP) assays. The total phenolics and flavonoid recovered by methanolic (MPE) and the water extract (AQPE) were ranged from 185 ± 12.45 to 298.00 ± 24.86 mg GAE (gallic acid equivalents)/gm and 23.05 ± 1.54 to 49.8 ± 2.14 quercetin (QE) mg/g, respectively. The EC50of herbal extracts ranged from 100 µg/ml (0.38 quercetin equivalents), for AQPE, 168 µg/ml (0.80 quercetin equivalents), for MPE. The phenolic profile in the methanolic extracts was investigated by chromatographic (HPLC) method. About 5 different flavonoids, phenolic acids, and their derivatives including quercetin (1), rutin (2), gallic acid (3), ellagic acid (4), and punicalagin as a major ellagitannin (5) have been identified. Among both extracts, methanolic extract was the most effective. This report may be the first to show nutritive content and correlation analysis to suggest that phenols and flavonoids might contribute the high antioxidant activity of this fruit peel and establish it as a valuable natural antioxidant source applicable in the health food industry.

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Harnessing the medicinal properties of Cussonia barteri Seem. (Araliaceae) in drug development. A review

Herba Polonica ◽

10.2478/hepo-2018-0018 ◽

2018 ◽

Vol 64 (3) ◽

pp. 50-61 ◽

Cited By ~ 1

Author(s):

Ighodaro Igbe ◽

Osaze Edosuyi ◽

Agbonlahor Okhuarobo

Keyword(s):

Drug Development ◽

Antioxidant Activities ◽

Biological Activities ◽

Stem Bark ◽

Deciduous Tree ◽

Drug Candidates ◽

Medicinal Properties ◽

Phytochemical Constituents ◽

Anti Inflammatory ◽

Novel Drug

Summary Cussonia barteri Seem (Araliaceae) is a deciduous tree growing in savannah of Africa. Ethnomedicinally, it is used in Africa as an analgesic, anti-malarial, anti-inflammatory, anti-anaemic, anti-diarhoea, anti-poison, ani-pyschotic and anti-epileptic agent. This review provides a brief summary on the phytochemical screenings, ethnomedicinal and pharmacological applications of various parts of C. barteri. Leaves, stem bark and seed of C. barteri have been shown to be rich in saponins, flavonoids, phenols, sugars and alkaloids. Some of these constituents have been isolated and elucidated from C. barteri. Several compounds isolated from plant include triterpenes, saponins, polyenyne and quinic esters. Phytochemical constituents are also partly responsible for biological activities of C. barteri. Extracts and components isolated from the plant have demonstrated neuropharmacological, anti-larvicidal, anti-microbial, anti-inflammatory and antioxidant activities. Overall, the insights provided by this review reinforce the potential of C. barteri for drug development and create the need for further scientific probe of constituents of the plant with the aim of developing novel drug candidates.

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Phytochemical, cytotoxicity and antioxidant studies on the stem bark of Piper arborescens

Malaysian Journal of Fundamental and Applied Sciences ◽

10.11113/mjfas.v13n4.706 ◽

2017 ◽

Vol 13 (4) ◽

pp. 840-845 ◽

Cited By ~ 1

Author(s):

Nicholas Anak Daniel ◽

Fasihuddin Badruddin Ahmad ◽

Zaini Assim ◽

Chua Hun Pin

Keyword(s):

Brine Shrimp ◽

Antioxidant Activities ◽

Stem Bark ◽

Artemia Salina ◽

Methyl Eugenol ◽

Gas Chromatography Mass Spectrometry ◽

Caryophyllene Oxide ◽

Phytochemical Study ◽

Crude Extracts ◽

High Cytotoxicity

Phytochemical study on the stem bark of P. arborescens has isolated six secondary metabolites from the crude extracts by using column chromatography, while identification was performed by using Gas Chromatography - Mass Spectrometry (GC-MS), Nuclear Magnetic Resonance (NMR) and Fourier Transform Infrared (FTIR). The isolated metabolites were identified as caryophyllene oxide (1), α-bisabolol (2), benzamide 2-(methylamino) (3), 2-ethylpiperidine (4), piperine (5) and methyl eugenol (6). Toxicity test on the four crude extracts of P. arborescens shows a high cytotoxicity against Artemia salina brine shrimp with LC50 values ranging from 13.12 to 58.70 µg/mL. Greater cytotoxicity of the crude extracts of P. arborescens indicated the presence of potent cytotoxic components in this Piper spp. Antioxidant assay of P. arborescens against 2-diphenyl-1-picrylhydrazyl (DPPH) indicated moderate antioxidant activities of methanol, dichloromethane, chloroform and hexane crude extracts with EC50 values of 21.68, 23.82, 32.88 and 36.88 µg/mL, respectively.

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In vivo and In vitro Antioxidant Activities of Aqueous and Ethanol Stem Bark Extracts of Vitex doniana on Doxorubicin-induced Oxidative Stress in Rats

Asian Journal of Research in Biochemistry ◽

10.9734/ajrb/2021/v8i230178 ◽

2021 ◽

pp. 44-55

Author(s):

Mohammed Aliyu Sulaiman ◽

Daniel Dahiru ◽

Mohammed Auwal Ibrahim ◽

Ahmed Ibrahim Hayatu

Keyword(s):

Oxidative Stress ◽

Antioxidant Activities ◽

Ischemia Reperfusion ◽

Oral Treatment ◽

Stem Bark ◽

Albino Rats ◽

Associated Diseases ◽

Vitex Doniana

Background: Oxidative stress is involved in the pathogenesis of hypertension, myocardial ischemia-reperfusion injury, atherosclerosis, muscular dystrophy, aging and other associated diseases. Vitex doniana is used in Adamawa, northern Nigeria to treat oxidative stress associated diseases. However, the antioxidative effects of the plant have not been scientifically examined in oxidative stress experimental animal models. The aim of this study is to investigate the in vitro and in vivo antioxidant activities of aqueous and ethanol stem bark extracts of Vitex doniana in oxidative stress model of rats. Methods: The study used 35 adult albino rats weighing 175 ± 25 g, of which 30 were induced with oxidative stress by intraperitoneal injection of doxorubicin (10 mg/kg) for three consecutive days. Animals were treated by oral administration of silymarin (100 mg/kg) and Vitex doniana aqueous or ethanol extract (100 mg/kg and 200 mg/kg) for 14 consecutive days before they were sacrificed on the 15th day and blood was analyzed for biochemical indices of oxidative stress. Results: The results of the phytochemistry showed the presence of alkaloids, tannins, flavonoids, steroids, phenols, saponins, terpenoids, glycosides: and total flavonoids (52.70 ± 1.60 mg/ml and 75.40 ± 0.80 mg/ml), total phenols (21.45 ± 1.54 mg/ml and 26.50 ± 1.22 mg/ml) for aqueous and ethanol stem bark extracts respectively. The extracts scavenged DPPH radical, reduced Fe3+ and inhibited lipid peroxidation. Doxorubicin significantly (p<0.05) lowered the levels of SOD, CAT, GR and TAS and significantly (p<0.05) but, increased the level of LPO. Oral treatment with Vitex doniana extracts significantly (p<0.05) increased the activities of CAT, GR, SOD and TAS while LPO was significantly (p<0.05) lowered. Vitex doniana stem bark extracts significantly (p<0.05) improved the biochemical derangements observed in the induced untreated animals in comparable manner to that of Silymarin. Conclusion: The present study provides the scientific rationale for the use of Vitex doniana stem bark in traditional medicine and has a viable antioxidative capacity both in vitro and in vivo.

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Spondias purpurea L. Stem Bark Extract: Antioxidant and in vitro Photoprotective Activities

Journal of the Brazilian Chemical Society ◽

10.21577/0103-5053.20210082 ◽

2021 ◽

Author(s):

Francisco Alessandro Rodrigues ◽

Priscylla Giffony ◽

Sarah dos Santos ◽

Jhonyson Guedes ◽

Maria Elenir Ribeiro ◽

...

Keyword(s):

Active Ingredient ◽

Antioxidant Activities ◽

Stem Bark ◽

Ultra Performance Liquid Chromatography ◽

Bark Extract ◽

Protection Factor ◽

Phenolic Constituents ◽

Stem Bark Extract ◽

Spondias Purpurea

Plant phenolic extracts are widely recognized as an important source of natural antioxidant substances and potential compounds for cosmetic formulations. This study aimed to evaluate the chemical profile, photoprotective and antioxidant activities of stem bark extract of Spondias purpurea L. (ciriguela) and its application in photoprotective formulations. Thirty phenolic constituents were annotated by ultra-performance liquid chromatography coupled with an electrospray ionization quadrupole time-of-flight mass spectrometry in mode negative (UPLC‑QTOF-MS2). The stem bark extract antioxidant and chelation potential, expressed in half maximal inhibitory concentration (IC50), showed 6.25 and 352.22 μg mL−1, respectively. The phenolic extract was used as an active ingredient in six sunscreen formulations, with concentrations ranging from 0.2 to 10%. The ultraviolet (UV) protection properties of the formulations were evaluated by sun protection factor (SPF) values obtained in 0.2 mg mL−1 (0.495 to 2.27) and 2.0 mg mL−1 (2.29 to 15.87). The SPF value for the extract (14.37 and 26.16) was high, but there was a reduction in the base formulation. However, these results suggested that the bioactive extracted of stem bark of Spondias purpurea L. has interesting potential to reduce the damage caused by UV radiation and may be utilized as an active ingredient in a sunscreen formulation.

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Antimicrobial and Antioxidant Activities and Phenolic Profile of Eucalyptus globulus Labill. and Corymbia ficifolia (F. Muell.) K.D. Hill & L.A.S. Johnson Leaves

Molecules ◽

10.3390/molecules20034720 ◽

2015 ◽

Vol 20 (3) ◽

pp. 4720-4734 ◽

Cited By ~ 31

Author(s):

Ștefan Dezsi ◽

Alexandru Bădărău ◽

Cristina Bischin ◽

Dan Vodnar ◽

Radu Silaghi-Dumitrescu ◽

...

Keyword(s):

Eucalyptus Globulus ◽

Antioxidant Activities ◽

Phenolic Profile

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Antibacterial, Antioxidant and Anti-Inflammatory Potential of the Different Extracts of Holoptelia Integrifolia

International Journal of Engineering and Advanced Technology - Regular Issue ◽

10.35940/ijitee.a9584.119119 ◽

2019 ◽

Vol 9 (2) ◽

pp. 5234-5240

Keyword(s):

Antioxidant Activities ◽

Radical Scavenging ◽

Ethanolic Extract ◽

Stem Bark ◽

Inflammatory Activity ◽

Diffusion Method ◽

Albino Rats ◽

Nitric Oxide Radical ◽

Anti Inflammatory ◽

Wistar Albino Rats

Medicinal plants are play significant impact in the personal medicine for most of the people all over the world as an alternative live saving medicines and most of their medicinal properties are well known for anticancer activity. The different extracts of Holoptelea integrifolia (H.integrifolia) leaves, stem bark and fruits were studied as a potent natural source of antimicrobial, antioxidant and wound healing potential. This work was carried out to evaluate antimicrobial, antioxidant and anti-inflammatory activity of different extracts of H.integrifolia. The antimicrobial activity of the H.integrifolia ethanolic extract was studied against five fungal and bacterial strains by utilizing the agar well diffusion method and MIC. Among several strain, the ethanolic extract of fruit has shown higher antimicrobial inhibition zone as 9.25-16 mm compare to other two extracts of stem and leaves as 10- 13.25 mm and 6-10.2 mm respectively. The antioxidant activities for different extract were also determined by DPPH free radical assay, Hydroxyl Radical Scavenging and Nitric Oxide Radical Scavenging Activity method. The anti-inflammatory activity also estimated basedon formalin induced paw edema method on Wistar albino rats. The different extracts of leaves, stem bark and fruit parts of Holoptelea integrifolia were estimated for in vivo anti-inflammatory activity against the animal model of female Wistar albino rats. The results of anti-inflammatory activity revealed that the Ethanol extracts showed vital and dose-dependent anti-inflammatory effects. Our findings revealed that aerial parts of H.integrifolia contais potential antimicrobial, antioxidant and anti-inflammatory compounds, which expose the medicinal potential of the selected plant could be a significant drug candidates against microbial, oxidative and inflammation-related pathological processes as a future alternative medicine.

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Antifungal Activity and Phytochemical Analysis of Column Chromatographic Fractions of Stem Bark Extracts of Ficus sycomorus L. (Moraceae)

Journal of Plant Sciences ◽

10.3923/jps.2007.209.215 ◽

2007 ◽

Vol 2 (2) ◽

pp. 209-215 ◽

Cited By ~ 4

Author(s):

S.W. Hassan ◽

M. Lawal ◽

B.Y. Muhammad ◽

R.A. Umar ◽

L.S. Bilbis ◽

...

Keyword(s):

Antifungal Activity ◽

Stem Bark ◽

Phytochemical Analysis ◽

Ficus Sycomorus ◽

Column Chromatographic

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Phenolic Profile and Bioactive Potential of Stems and Seed Kernels of Sweet Cherry Fruit

Antioxidants ◽

10.3390/antiox9121295 ◽

2020 ◽

Vol 9 (12) ◽

pp. 1295 ◽

Cited By ~ 1

Author(s):

Sílvia Afonso ◽

Ivo Vaz Oliveira ◽

Anne S. Meyer ◽

Alfredo Aires ◽

Maria José Saavedra ◽

...

Keyword(s):

Phenolic Compounds ◽

High Performance ◽

Sweet Cherry ◽

Antioxidant Activities ◽

Antibacterial Activities ◽

Gram Positive ◽

Phenolic Profile ◽

Bioactive Potential ◽

Sweet Cherry Cultivars

Every year, large quantities of stems and pits are generated during sweet cherry processing, without any substantial use. Although stems are widely recognized by traditional medicine, detailed and feasible information about their bioactive composition or biological value is still scarce, as well as the characterization of kernels. Therefore, we conducted a study in which bioactivity potential of extracts from stems and kernels of four sweet cherry cultivars (Early Bigi (grown under net cover (C) and without net cover (NC)), Burlat, Lapins, and Van) were examined. The assays included antioxidant (by 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic) acid (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH) and β-carotene-linoleic acid bleaching assays), and antibacterial activities against important Gram negative and Gram positive bacterial human isolates. Profile and individual phenolic composition of each extract were determined by High-performance liquid chromatography (HPLC) analysis. Extracts from stems of cv. Lapins and kernels of Early Bigi NC presented high levels of total phenolics, flavonoids, ortho-diphenols and saponins. Excepting for cv. Early Bigi NC, major phenolic compounds identified in stems and kernels were sakuranetin and catechin, respectively. In cv. Early Bigi NC the most abundant compounds were ellagic acid for stems and protocatechuic acid for kernels. In all extracts, antioxidant activities showed a positive correlation with the increments in phenolic compounds. Antimicrobial activity assays showed that only stem’s extracts were capable of inhibiting the growth of Gram positive isolates. This new data is intended to provide new possibilities of valorization of these by-products and their valuable properties.

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