scholarly journals Freeze-drying of Beauveria bassiana suspended in Hydroxyethyl cellulose based hydrogel as possible method for storage: Evaluation of survival, growth and stability of conidial concentration before and after processing

2021 ◽  
pp. 100283
Author(s):  
Marco Friuli ◽  
Paola Nitti ◽  
Chioma I. Aneke ◽  
Christian Demitri ◽  
Claudia Cafarchia ◽  
...  
Keyword(s):  
Beauveria Bassiana ◽  
Freeze Drying ◽  
Hydroxyethyl Cellulose ◽  
Before And After ◽  
Conidial Concentration

scholarly journals Selection, assessment of virulence to Alphitobius diaperinus, and Pr1 enzyme production of Beauveria bassiana (Bals.) Vuill. isolates cultured at stress temperatures

2015 ◽  
Vol 36 (6) ◽  
pp. 3529
Author(s):  
Kelly Christiane Constanski ◽  
Janaina Zorzetti ◽  
Pedro Manuel Oliveira Janeiro Neves
Keyword(s):  
Beauveria Bassiana ◽  
Alphitobius Diaperinus ◽  
Control Programs ◽  
Colony Forming Units ◽  
Significant Difference ◽  
Before And After ◽  
Insect Mortality ◽  
Selection Test ◽  
High And Low Temperatures ◽  
Fungus Beauveria Bassiana

The entomopathogenic fungus Beauveria bassiana is a promising agent for use in insect control. Its pathogenic activity, as well as other factors such as temperature that can interfere with its development, should be assessed, thus, establishing the foundations for B. bassiana use in biological control programs. The objective of this study was to select and induce tolerance of B. bassiana isolates to high and low temperatures and to assess their virulence before and after exposure to those temperatures. A pre-selection test was performed, in which the tolerance of isolates to stress temperatures was tested and compared to the ideal growth temperature of 25 °C for this organism. For the isolates/temperature combinations resulting in growth, conidia germination and colony-forming units (CFUs) were assessed. The isolates Unioeste 4 and Unioeste 40 exhibited >95% germinated conidia at 16 and 31 °C. Thereafter, they underwent four consecutive passages at maximum and minimum tolerated temperatures (10 and 37 °C). A significant difference in germination was observed between the two isolates at all temperatures tested. More CFUs were observed for Unioeste 4 compared to Unioeste 40 at all temperatures, and in the case of the latter, there was no difference in CFU formation at 10 and 25 °C. For both isolates, decreased vegetative growth was observed at 37 °C. Recovery of virulence was observed in both isolates, as determined by insect mortality. No relationship was observed between production of the enzyme Pr1 and the virulence of the isolates.

Preservation of the Moringa oleifera Constituents by Freeze-drying

2019 ◽  
pp. 1-6
Author(s):  
Semirames Do N. Silva ◽  
Francisco A. C. Almeida ◽  
Josivanda P. Gomes ◽  
Newton C. Santos ◽  
Damião J. Gomes ◽  
...  
Keyword(s):  
Moisture Content ◽  
Freeze Drying ◽  
Moringa Oleifera ◽  
Chemical Constituents ◽  
Physical Parameters ◽  
Edible Plant ◽  
Agricultural Engineering ◽  
Physico Chemical ◽  
Before And After ◽  
Processing And Storage

Aims: Moringa oleifera is an edible plant. A wide variety of nutritional and medicinal virtues have been attributed to its roots, barks, leaves, flowers, fruits and seeds. The objective of this research was to evaluate the preservation of the constituents of the powder obtained from the moringa seeds by freeze-drying comparing it with the in natura (natural extract). Place and Duration of Study: The work was conducted at the Laboratory of Processing and Storage of Agricultural Products, Department of Agricultural Engineering, Federal University of Campina Grande, Brazil, in the period from August to November 2018. Methodology: The seeds were peeled and macerated manually. Freeze-drying was done in a Liotop® L101 benchtop freeze drier. After dehydration the samples were disintegrated and the physical and physico-chemical constituents were evaluated before and after freeze-drying in terms of apparent density, real density, porosity, compacted density, compressibility index, Hausner factor, solubility, moisture content and activity, ash, titratable total acidity, pH, protein, lipids and carbohydrates. Results: The in natura powder presented better results for the physical analyzes of the densities: apparent, real and compacted, however, it was observed that for the other physical parameters and physicochemical constituents the freeze-drying promoted the preservation of these in front of the in natura. Freeze-drying caused a significant reduction in moisture content, pH and lipid activity, making the powders more stable and contributing to the maintenance of their physico-chemical qualities. The inverse was observed for the ash, protein and carbohydrate contents, where freeze-drying promoted increases in their contents. Conclusion: Freeze-drying presents as an appropriate method in the preservation of moringa constituents, with emphasis on physicochemical.

Effect of NaH2PO4, Na2HPO4, MgSO4, and Ascorbic Acid on Survival of Lactobacillus acidophilus during Freeze-Drying

2012 ◽  
Vol 568 ◽  
pp. 279-282
Author(s):  
He Chen ◽  
Zhen Xing Ma ◽  
Guo Wei Shu ◽  
Tao Qin
Keyword(s):  
Ascorbic Acid ◽  
Survival Rate ◽  
Lactobacillus Acidophilus ◽  
Freeze Drying ◽  
Protective Agent ◽  
Survival Ratio ◽  
Protective Agents ◽  
Viable Cells ◽  
Before And After

Effect of NaH2PO4, Na2HPO4, MgSO4, and Ascorbic acid on survival of Lactobacillus acidophilus was investigated before and after freeze-drying. Editing different concentrations of protective agents respectively during freeze-drying. After completion of the pilot, the survival ratio of Lactobacillus acidophilus and the number of viable cells were being measured. Results were as follows: the highest survival rate and the largest number of viable cells was Na2HPO4as the protective agent.

scholarly journals Comparative Study of Dark Beer Before and After Freeze Drying Rehydration Cycle

2018 ◽  
Vol 72 (2) ◽  
pp. 123-127
Author(s):  
Ingmārs Cinkmanis ◽  
Guntis Gailums ◽  
Sanita Vucāne
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Phenolic Compounds ◽  
Organic Acids ◽  
High Performance ◽  
Freeze Drying ◽  
Gas Chromatography Mass Spectrometry ◽  
Before And After

Abstract The aim of the study was to compare dark beer before and after a freeze drying rehydration cycle. The pH, colour, and concentrations of extract, organic acids, and phenolic compounds were determined. Dark beer before and after freeze drying was analysed using standard brewing analysis methods of the Mitteleuropäische Brautechnische Analysenkommision (MEBAK) and two types of chromatography: high-performance liquid chromatography (HPLC) and gas chromatography mass spectrometry (GC/MS). Increase of pH was not observed in dark beer after freeze drying. Dissolved powdered beer in deionised water was clear, dark brown, without sediments, and reproduced the original commercially available dark beer, with original extract substance content 8.88%. Concentrations of the following organic acids were determined: oxalic, tartaric, quinic, malic, ascorbic, lactic, acetic, citric, fumaric, and succinic. Volatile compounds by GC/MS analysis were isolated from dark beer before and after freeze drying.

Effect of Four Materials Including Glycine, Arginine, Proline and Sodium Glutamate on Survival of Bifidobacterium bifidum during Freeze-Drying

2013 ◽  
Vol 676 ◽  
pp. 93-96
Author(s):  
Qi Ma ◽  
Tao Qin ◽  
He Chen ◽  
Guo Wei Shu
Keyword(s):  
Survival Rate ◽  
Freeze Drying ◽  
Bifidobacterium Bifidum ◽  
Sodium Glutamate ◽  
Viable Cells ◽  
Before And After

Effect of four materials including glycine, arginine, proline and sodium glutamate on survival of Bifidobacterium bifidum during freeze-drying was studied by measuring the viable counts of before and after freeze-drying. The addition of glycine, arginine, proline and sodium glutamate (%, w/v) were all 1.0%, 2.0%, 3.0%，4.0% and 5.0%. Results were as follows: addition of glycine, arginine, proline and sodium glutamate into B. bifidum paste could significantly improve the survival rate of B. bifidum and the number of viable cells of lyophilized powder. The optimal concentrations of glycine, arginine, proline and sodium glutamate were 4.0%, 4.0%, 3.0% and 2.0%, the survival of B. bifidum was 87.49%, 84.28%, 67.50% and 69.01%, respectively.

scholarly journals Influence of Hydroxyethyl Cellulose Treatment on the Mechanical Properties of Jute Fibres, Yarns, and Composites

2013 ◽  
Vol 2013 ◽  
pp. 1-6
Author(s):  
Ranajit K. Nag ◽  
Andrew C. Long ◽  
Michael J. Clifford
Keyword(s):  
Mechanical Properties ◽  
Tensile Strength ◽  
Room Temperature ◽  
Failure Load ◽  
Tap Water ◽  
Tensile Modulus ◽  
Hydroxyethyl Cellulose ◽  
Yarn Strength ◽  
Before And After ◽  
Increased Strength

Jute yarns were treated by tap water with and without tension at room temperature for 20 minutes and then dried. Fibre and yarn strength were measured before and after treatment. Unidirectional (UD) composites were made by both treated and untreated yarns with and without applying hydroxyethyl cellulose (HEC) as size material. Water-treated jute yarns without tension and composites made of those yarns showed decreased strength, and water treated jute yarns with tension and composites made of those yarns showed increased strength with respect to raw yarns and composites made of raw yarns. However, no specific trend was noticed for fibre tensile strength and tensile modulus. HEC sized yarns showed up to 12% higher failure load with respect to unsized yarns, and composites made of HEC sized yarns showed up to 17% and 12% increase in tensile strength and tensile modulus, respectively, compared to composites made of similar types of unsized yarns.

scholarly journals Entomopathogens of Anαcridium αegyptium L. in Crete

2017 ◽  
Vol 14 ◽  
pp. 5
Author(s):  
Ν. Ε. Roditakis ◽  
D. Kollaros ◽  
Α. Legakis
Keyword(s):  
Beauveria Bassiana ◽  
Comparative Studies ◽  
Metarhizium Anisopliae ◽  
Entomopathogenic Fungus ◽  
Serial Dilution ◽  
Local Isolate ◽  
Conidial Concentration ◽  
First Time ◽  
Fungus Beauveria Bassiana ◽  
Metarhizium Anisopliae Var Acridum

The entomopathogenic fungus Beauveria bassiana (Bals.) Vuil. was recorded for the first time on Anacridium aegyptium L. in Crete. The insects were fed on pieces of leaf subjected to a serial dilution of spores over three to four orders of magnitute. Comparative studies on the virulence of B. bassiana (I 91612 local isolate) and Metarhizium anisopliae var. acridum (IMI 330189 standard isolate of IIBC) showed that M. anisopliae var. acridum was more virulent than B. bassiana at a conidial concentration lower or equal to 106 per ml while they were similarly virulent on first stage nymphs at 107 conidia per ml.

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