Chemical composition of Moringa (Moringa oleifera) root powder solution and effects of Moringa root powder on E. coli growth in contaminated water

Essential oils (EOs) from Citrus are the main by-product of Citrus-processing industries. In addition to food/beverage and cosmetic applications, citrus EOs could also potentially be used as an alternative to antibiotics in food-producing animals. A commercial citrus EO—Brazilian Orange Terpenes (BOT)—was fractionated by vacuum fractional distillation to separate BOT into various fractions: F1, F2, F3, and F4. Next, the chemical composition and biological activities of BOT and its fractions were characterized. Results showed the three first fractions had a high relative amount of limonene (≥10.86), even higher than the whole BOT. Conversely, F4 presented a larger relative amount of BOT’s minor compounds (carvone, cis-carveol, trans-carveol, cis-p-Mentha-2,8-dien-1-ol, and trans-p-Mentha-2,8-dien-1-ol) and a very low relative amount of limonene (0.08–0.13). Antibacterial activity results showed F4 was the only fraction exhibiting this activity, which was selective and higher activity on a pathogenic bacterium (E. coli) than on a beneficial bacterium (Lactobacillus sp.). However, F4 activity was lower than BOT. Similarly, F4 displayed the highest antioxidant activity among fractions (equivalent to BOT). These results indicated that probably those minor compounds that detected in F4 would be more involved in conferring the biological activities for this fraction and consequently for the whole BOT, instead of the major compound, limonene, playing this role exclusively.

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Bacteria Detection and Differentiation Using Impedance Flow Cytometry

Sensors ◽

10.3390/s18103496 ◽

2018 ◽

Vol 18 (10) ◽

pp. 3496 ◽

Cited By ~ 21

Author(s):

Casper Clausen ◽

Maria Dimaki ◽

Christian Bertelsen ◽

Gustav Skands ◽

Romen Rodriguez-Trujillo ◽

...

Keyword(s):

Drinking Water ◽

Real Time ◽

Electrical Response ◽

Solid Particles ◽

Electrical Impedance Spectroscopy ◽

Contaminated Water ◽

E Coli ◽

Two Samples ◽

Microbiological Control ◽

Frequency Phase

Monitoring of bacteria concentrations is of great importance in drinking water management. Continuous real-time monitoring enables better microbiological control of the water and helps prevent contaminated water from reaching the households. We have developed a microfluidic sensor with the potential to accurately assess bacteria levels in drinking water in real-time. Multi frequency electrical impedance spectroscopy is used to monitor a liquid sample, while it is continuously passed through the sensor. We investigate three aspects of this sensor: First we show that the sensor is able to differentiate Escherichia coli (Gram-negative) bacteria from solid particles (polystyrene beads) based on an electrical response in the high frequency phase and individually enumerate the two samples. Next, we demonstrate the sensor’s ability to measure the bacteria concentration by comparing the results to those obtained by the traditional CFU counting method. Last, we show the sensor’s potential to distinguish between different bacteria types by detecting different signatures for S. aureus and E. coli mixed in the same sample. Our investigations show that the sensor has the potential to be extremely effective at detecting sudden bacterial contaminations found in drinking water, and eventually also identify them.

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Geo-Assessment of Chemical Composition and Nutritional Evaluation of Moringa oleifera Seeds in Nutrition of Broilers

Journal of Agricultural Science ◽

10.5539/jas.v6n4p119 ◽

2014 ◽

Vol 6 (4) ◽

pp. 119 ◽

Cited By ~ 3

Author(s):

A. Annongu ◽

O. R. Karim ◽

A. A. Toye ◽

F. E. Sola-Ojo ◽

R. M. O. Kayode ◽

...

Keyword(s):

Body Weight ◽

Weight Gain ◽

Chemical Composition ◽

Feed Intake ◽

Moringa Oleifera ◽

The Body ◽

Feed Consumption ◽

Seed Meal ◽

Mineral Matter ◽

Inclusion Level

Chemical composition of Moringa oleifera seeds obtained from the middle belt of Nigeria, Benue State, was determined and the seed was blended to form a seed meal. The Moringa oleifera Seed Meal, MOSM was included in diets at graded levels of 2.50, 5.00 and 7.50% and the dietary performance of the broiler chicks on the test diets was compared with that of a corn-soy reference diet. Results on the chemical/nutritional composition of MOSM showed that the full-fat seeds contained (%) on proximate basis, reasonable concentration of 90.38 dry matter, 25.37 crude protein, 14.16 crude fat, 4.03 mineral matter, 30.64 crude fiber, 25.80 soluble carbohydrate and 5.79 kcal/g gross energy. Analyses also gave appreciable quantities of the water and fat soluble vitamins, macro - and micro-minerals. Feeding chicks with the seed meal at graded levels in diets resulted in decrease in feed intake and body weight gain as the inclusion level increased in diets relative to the conventional diet (p < 0.05). Reduction in feed consumption could be attributed to the full-fat nature of the seed meal used which might have imparted extra-caloric effect in the test diets and slowed digestion and absorption as the analyzed nutrients content of diets. A higher ether extract value on Moringa based diets relative to the control diet was obtained. Phytochemical composition of Moringa namely phenols including tannins, saponins, phytate, cyanogenic glucoside, glucosinolates and other numerous chemical constituents affected the body weight of the chicks negatively with increasing dietary MOSM. Decrease in weight gain following increase in dietary seed meal could also be due to decrease in feed intake as a result of the bitter taste of alkaloids, saponins, acting in concert with the other Moringa phytotoxins in test diets. Survival rate (100%) was not affected indicating that the level of highest inclusion in this study (7.50%) was not fatal to the experimental animal models. Further research is progressing to ascertain the highest inclusion level possible to elicit fatality and attempts to detoxify or treat the seed meal before feeding to animals.

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Using Recombinant E. coli Displaying Surface Heavy Metal Binding Proteins for Removal of Pb2+ from Contaminated Water

Journal of Bioremediation & Biodegradation ◽

10.4172/2155-6199.1000442 ◽

2018 ◽

Vol 09 (03) ◽

Cited By ~ 1

Author(s):

Mohamed Shehata ◽

Ken ichi Yamazaki

Keyword(s):

Heavy Metal ◽

Metal Binding ◽

Binding Proteins ◽

Contaminated Water ◽

E Coli ◽

Heavy Metal Binding

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Removal of Methylene blue, Escherichia coli and Pseudomonus aeruginosa by Adsorption Process of Activated Carbon Produced from Moringa oleifera Bark

Malaysian Journal of Science Health & Technology ◽

10.33102/mjosht.v7i.105 ◽

2021 ◽

Author(s):

Md. Shahin Azad ◽

Syaza Azhari ◽

Mohd Sukri Hassan

Keyword(s):

Escherichia Coli ◽

Methylene Blue ◽

Activated Carbon ◽

Moringa Oleifera ◽

Adsorption Process ◽

Diffusion Mechanism ◽

Bacterial Load ◽

Batch Adsorption ◽

Maximum Adsorption Capacity ◽

E Coli

The utilization of biopolymer derived from Moringa oleifera bark using ZnCl2 and H2SO4 as activating agents for eliminating Methylene blue, Escherichia coli and Pseudomonas aeruginosa from producing wastewater. In this study, Methylene blue and both bacteria were effectively adsorbed by activated carbon with lowest dosage. The activated carbon was prepared from natural-by product of Moringa oleifera bark by pyrolysis in a furnace at 700°C for 1 h. The characteristics of activated carbon have been determined using Scanning Electron Microscopy (SEM), Brunauer-Emmett-Teller (BET), pHzpc (zero point charge), and FTIR spectroscopy. The obtained result were closely fitted with Freundlich isotherm model and adsorption kinetics follow the pseudo-second order model with the highest value of correlation coefficient (R2~1). Adsorption quantity was dose dependent and bacteria were maximum adsorbed using 10 mg of activated carbon as well as 25mg for methylene blue. The maximum adsorption capacity showed within 1 hour. The bacterial load was reduced by 98% for E. coli, 96% for P. aeruginosa as well as methylene blue reduced 94.2% from aqueous solution using batch adsorption methods. Adsorption process controlled by film diffusion mechanism. These result proposed that the activated carbon of Moringa oleifera can be used as a good adsorbent for the removal of Methylene blue, E. coli and P. aeruginosa.

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The Potential Impact of Moringa oleifera for Diminishing the Microbial contamination and Prolonging the Quality and Shelf-Life of Chilled Meat

Journal of Pure and Applied Microbiology ◽

10.22207/jpam.15.2.37 ◽

2021 ◽

Author(s):

Mohamed Hamada ◽

Mabrouk Abd Eldaim ◽

Said I. Fathalla ◽

Ayman El Behiry ◽

Mohamed Alkafafy

Keyword(s):

Shelf Life ◽

Moringa Oleifera ◽

Thiobarbituric Acid ◽

Control Group ◽

Standard Diet ◽

Breast Meat ◽

E Coli ◽

Potential Impact ◽

Cadmium And Lead ◽

Quality And Shelf Life

This study was implemented to assess the mechanism by which Moringa oleifera leaf extract (MOLE) improves the quality and prolongs shelf-life of the broilers’ breast meat. Ninety Cobb chicks were randomly allocated to 3 groups. A control group received the standard diet, whereas the other two groups received diets containing MOLE at the doses of 250 and 500 mg/kg for 21 days. Inclusion of MOLE in broilers diet significantly reduced the detrimental changes in the overall sensory attribute scores, characteristic color and odor, and the loss of breast muscle elasticity during storage. Furthermore, it significantly reduced concentrations of thiobarbituric acid, total volatile nitrogen, non-esterified fatty acids, and peroxide, during storage compared to the control samples. No effect on the concentrations of heavy metals, such as copper, cadmium, and lead, was observed. Decomposition of samples was delayed as indicated by lower pH values and higher sensory scores at 4 and 6 days of storage in the MOLE groups. Reduced contamination with E. coli and Salmonella species indicated an antibacterial effect of MOLE. Finally, the present study highlights that MOLE supplementation may play a role in improving quality and shelf-life of the chilled breast meat in broilers.

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