Major phenolic compounds, antioxidant and antibacterial activities of Anthemis praecox Link aerial parts

Abstract Background Based on our previous ethnobotanical survey, the non-investigated Saharan plant Anabasis aretioides Coss. & Moq., growing in the region of Errachidia, was selected for pharmacological investigation. In Moroccan traditional medicine, A. aretioides is being used for diabetes treatment. Thus, the current work aims at evaluating the antidiabetic, antioxidant, and antibacterial activities of the plant in relation to the digestive tract. Methods The different parts of the plant (aerial parts, roots, seeds) were extracted with methanol (MeOH) and screened in enzymatic assays for their inhibitory potential against α-amylase and α-glucosidase, as well as antioxidant and antibacterial activities. Furthermore, the phenolic compounds were analyzed using HPLC-DAD-QTOF-MS. Results The MeOH extracts of A. aretioides aerial parts, roots, and seeds, respectively, inhibited α-amylase (IC50 of 3148.07 μg/mL, 2440.20 μg/mL, 3395.71 μg/mL) and α-glucosidase (IC50 of 2940.59 μg/mL, 3521.81 μg/mL, 3393.83 μg/mL). Moreover, compared to aerial parts and seeds, the plant roots exhibited higher antioxidant capacity and a potent reducing power. In resazurin microplate assay, the plant parts displayed a minimal inhibitory concentration (MIC) ranging from 7.81 mg/mL to 31.25 mg/mL. Chemical analysis revealed 25 phenolic compounds, with chlorogenic acid as the main phenolic compound in the aerial parts, hesperidin in roots, and quercitrin in seeds. Conclusion Anabasis aretioides cited for treatment of diabetes shows promising antioxidant and antibacterial properties, as well as an ability to inhibit digestive enzyme, including α-amylase and α-glucosidase. Thus, our results explain in part the traditional use of this Saharan medicine and open doors for further in vivo mechanistic and functional studies.

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UHPLC-PDA-ESI-MS profile of phenolic compounds in the aerial parts of Cuphea ingrata Cham. & Schltdl.

Natural Product Research ◽

10.1080/14786419.2020.1868463 ◽

2021 ◽

pp. 1-5

Author(s):

Danuta Sobolewska ◽

Aleksandra Owczarek ◽

Monika Olszewska ◽

Niraldo Paulino ◽

Irma Podolak ◽

...

Keyword(s):

Phenolic Compounds ◽

Esi Ms ◽

Aerial Parts

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Triterpenoidal and Phenolic Compounds Isolated from the Aerial Parts of Helicteres hirsuta and their Cytotoxicity on Several Cancer Cell Lines

Natural Product Communications ◽

10.1177/1934578x1901400103 ◽

2019 ◽

Vol 14 (1) ◽

pp. 1934578X1901400

Author(s):

Triet Thanh Nguyen ◽

Nadine Kretschmer ◽

Eva-Maria Pferschy-Wenzig ◽

Olaf Kunert ◽

Rudolf Bauer

Keyword(s):

Phenolic Compounds ◽

Cancer Cells ◽

Cell Lines ◽

Cancer Cell ◽

Human Cancer ◽

Cancer Cell Lines ◽

Moderate Activity ◽

Human Cancer Cell Lines ◽

Aerial Parts ◽

Helicteres Isora

Helicteres L. is one of the genera of the Sterculiaceae family with several remarkable activities. Previous studies revealed that terpenoids, flavonoids, and lignans are the dominant constituents of Helicteres species. However, information about this genus is scarce and unsystematic. Most of the phytochemical and pharmacological investigations have been mainly reported on Helicteres angustifolia and Helicteres isora, which are commonly used in China and Indonesia, respectively. In the present study, two terpenoids: 3β- O-acetylbetulinic acid (1) and simiarenol (2) together with three phenolic compounds: 4,4'-sulfinylbis(2-( tert-butyl)-5-methylphenol) (3), 7- O-methylisoscutellarein (4), 7,4'-di- O-methylisoscutellarein (5), and a mixture of stigmasterol and β-sitosterol were isolated and structurally elucidated from the aerial parts of Helicteres hirsuta Lour. Compounds 1-5 were tested for cytotoxicity on four human cancer cell lines: leukemia CCRF-CEM, breast MDA-MB-231, colon HCT116 and glioblastoma U251 cancer cells. Among them, compounds 1 and 3 showed moderate activity on CCRF-CEM and HCT116 cancer cells with IC50 values ranging from 14.6 to 31.5 μM (P < 0.05). This is the first time these compounds have been reported from this plant. To the best of our knowledge, compound 3 is novel in nature although it has been chemically synthesized before, and compounds 1, 2, and 4 are new to this plant family (Sterculiaceae).

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Chemical composition and antibacterial activities of essential oils from fruits of Melicope pteleifolia (Champ. Ex Benth.) T.G. Hartley grown in Lam Dong Province, Vietnam

ACADEMIA JOURNAL OF BIOLOGY ◽

10.15625/2615-9023/v42n3.13916 ◽

2020 ◽

Vol 42 (3) ◽

Author(s):

Hoang Thi Binh ◽

Tran Thi Bao Tram ◽

Do Ngoc Dai ◽

Vuong Thuy Tien ◽

Le Minh Tam ◽

...

Keyword(s):

Mass Spectrometry ◽

Chemical Composition ◽

Essential Oil ◽

Streptococcus Pyogenes ◽

Antibacterial Properties ◽

Antibacterial Activities ◽

Gas Chromatography Mass Spectrometry ◽

Diffusion Method ◽

Aerial Parts ◽

Agar Well Diffusion Method

In the present study, chemical composition and antibacterial properties of essential oil obtained from the aerial parts of the Melicope pteleifolia (Champ. ex Benth.) T.G Hartley in Dalat were evaluated. Essential oil was isolated through hydro-distillation. Twenty-nine constituents comprising 100% of the essential oil were characterized by gas chromatography/mass spectrometry (GC-MS) techniques. The major compounds in the essential oil were (+)-Sabinene (34.73%), Cis-α-bergamotene (13.15%), Z-α-trans-bergamotol (5.28%), β-mycrene (4.98%), and 1,3,6-octatriene, 3,7-dimethyl-(4.71%). Antibacterial activities of Melicope pteleifolia essential oil were investigated against Gram-positive and-negative bacteria. Results showed significant activities against Streptococcus pyogenes and Escherichia coli using an agar well diffusion method. The application of this essential oil in preventing and eliminating bacteria could be useful in fields as medicine and cosmetics.

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Antioxidant, Antibacterial Activities and Phytochemical Composition of the Aerial Parts, Stem and Roots of Gomphrena vaga Mart. from Northeast Brazil

Revista Virtual de Química ◽

10.21577/1984-6835.20210075 ◽

2021 ◽

Author(s):

Symone Costa de Castro ◽

Naiara Maia Oliveira ◽

Janiel Ramos de Oliveira ◽

Luciana Lucas Machado ◽

Katyúscya Veloso Leão ◽

...

Keyword(s):

Antibacterial Activities ◽

Northeast Brazil ◽

Phytochemical Composition ◽

Aerial Parts

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Chemical Composition, in vitro Cytotoxic, and Antibacterial Activities of Moroccan Medicinal Plants Euphorbia resinifera and Marrubium vulgare

Biointerface Research in Applied Chemistry ◽

10.33263/briac106.73437355 ◽

2020 ◽

Vol 10 (6) ◽

pp. 7343-7355

Keyword(s):

Medicinal Plants ◽

Solid Medium ◽

Chemical Compounds ◽

Antibacterial Activities ◽

Antibacterial Effects ◽

Animal Cells ◽

Aerial Parts ◽

Marrubium Vulgare ◽

Major Chemical

The purpose of our study was to bring elements of knowledge on the anticancer and antibacterial effects of two plants, widely used in Moroccan traditional pharmacopeia. These plants are: Marrubium vulgare and Euphorbia resinifera. The aerial parts of each plant were extracted successively with Hexane, Dichloromethane, and finally with methanol. The MTT-based method was applied to evaluate the cytotoxicity of the cancer cells: animal cells BSR and Vero and human cell RD. We evidenced an anticancer activity of the extract of the Marrubium vulgare and the dichloromethane extract of Euphorbia resinifera against the studied cells. The antibacterial activity was evaluated for three species of Rhodococcus: Rhodococcus equi, and strains GK1, GK3, grown in a liquid medium, or this medium solidified with agar. In the last test, the method is based on substance diffusion from well throughout the solid medium. The obtained profiles showed that the growth of bacteria is strongly inhibited by the extracts of Marrubium vulgare. However, the extracts of Euphorbia resinifera had no significant effect on bacterial growth. The chemical analysis of the raw extracts of Marrubium vulgare and Euphorbia resinifera by GC-MS analysis showed the presence of several major chemical compounds, mainly: octadecane, 2,6,10,15-tetramethylheptadecane, 2,6,10-trimethyltetradecane, linoleic acid, and deisopropylatrazine. Our observations an encouraging for deepening the studies of the extracts, in order to target better the active molecules, isolate them and to determine their mechanisms of action. The suggested studies would result in the much better valorization of these two medicinal plants.

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Gas chromatographic and antimicrobial analyses of phenolic compounds in Senna alata (L.) Roxb. (Fabales: Fabaceae)

Revista Brasileira de Gestão Ambiental e Sustentabilidade ◽

10.21438/rbgas.030609 ◽

2016 ◽

Vol 3 (6) ◽

pp. 355-365

Author(s):

Funmilayo Adelowo ◽

Oluwole Oladeji

Keyword(s):

Gas Chromatography ◽

Phenolic Compounds ◽

Antibacterial Activities ◽

Bioactive Metabolites ◽

Diffusion Method ◽

Chemical Components ◽

Bioactive Components ◽

Antimicrobial Substances ◽

Agar Disc ◽

Senna Alata

This present work is aimed at determining the chemical components in Senna alata (L.) Roxb. (Fabales: Fabaceae), that contains a large array of phenolic compounds which make it useful as antifungal and antibacterial. Despite a notable success in the recent researches, yet, the quantification of the chemical components has not been actually specified. There is a claim by local people that S. alata can cure fungi and bacteria related diseases. Therefore, there is a need to ascertain this claim by examining the bioactive components present in the plant. The Gas Chromatography (GC) analysis was performed using a Clarus 500 Perkin Elmer gas chromatography equipped with an Elite-5 capillary column with the addition of an absolute alcohol. The antimicrobial analysis was carried out using Agar Disc Diffusion Method. In the GC chromatogram, four prominent peaks were observed in leaf chromatogram, three peaks in flower chromatogram and five peaks in fruit chromatogram, respectively. The flower contained the highest amount of cinnamic acid (52.91%) and the fruit contained the least (17.03%). The presence of some bioactive metabolites could be responsible for the observed antifungal and antibacterial activities on the susceptible organisms studied. It could be concluded that this plant is natural sources of antimicrobial substances of high importance and are useful in chemotherapy.

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Phenolic Profile and Bioactive Potential of Stems and Seed Kernels of Sweet Cherry Fruit

Antioxidants ◽

10.3390/antiox9121295 ◽

2020 ◽

Vol 9 (12) ◽

pp. 1295 ◽

Cited By ~ 1

Author(s):

Sílvia Afonso ◽

Ivo Vaz Oliveira ◽

Anne S. Meyer ◽

Alfredo Aires ◽

Maria José Saavedra ◽

...

Keyword(s):

Phenolic Compounds ◽

High Performance ◽

Sweet Cherry ◽

Antioxidant Activities ◽

Antibacterial Activities ◽

Gram Positive ◽

Phenolic Profile ◽

Bioactive Potential ◽

Sweet Cherry Cultivars

Every year, large quantities of stems and pits are generated during sweet cherry processing, without any substantial use. Although stems are widely recognized by traditional medicine, detailed and feasible information about their bioactive composition or biological value is still scarce, as well as the characterization of kernels. Therefore, we conducted a study in which bioactivity potential of extracts from stems and kernels of four sweet cherry cultivars (Early Bigi (grown under net cover (C) and without net cover (NC)), Burlat, Lapins, and Van) were examined. The assays included antioxidant (by 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic) acid (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH) and β-carotene-linoleic acid bleaching assays), and antibacterial activities against important Gram negative and Gram positive bacterial human isolates. Profile and individual phenolic composition of each extract were determined by High-performance liquid chromatography (HPLC) analysis. Extracts from stems of cv. Lapins and kernels of Early Bigi NC presented high levels of total phenolics, flavonoids, ortho-diphenols and saponins. Excepting for cv. Early Bigi NC, major phenolic compounds identified in stems and kernels were sakuranetin and catechin, respectively. In cv. Early Bigi NC the most abundant compounds were ellagic acid for stems and protocatechuic acid for kernels. In all extracts, antioxidant activities showed a positive correlation with the increments in phenolic compounds. Antimicrobial activity assays showed that only stem’s extracts were capable of inhibiting the growth of Gram positive isolates. This new data is intended to provide new possibilities of valorization of these by-products and their valuable properties.

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Two New Prenylated Isoflavonoids from Erinacea anthyllis with Antioxidant and Antibacterial Activities

Natural Product Communications ◽

10.1177/1934578x1701200716 ◽

2017 ◽

Vol 12 (7) ◽

pp. 1934578X1701200 ◽

Cited By ~ 1

Author(s):

Soumia Mouffouk ◽

Laurence Marcourt ◽

Mohammed Benkhaled ◽

Kaouthar Boudiaf ◽

Jean-Luc Wolfender ◽

...

Keyword(s):

Antioxidant Activities ◽

Optical Rotation ◽

2D Nmr ◽

Antibacterial Activities ◽

Total Phenolic ◽

Aerial Parts ◽

Whole Plant ◽

The Family ◽

Chemotaxonomic Markers ◽

Phenolic And Flavonoid

Two new prenylated isoflavonoids, namely Erinasone A and Erinasone B along with 19 known secondary metabolites, including twelve isoflavonoids, three polyphenols, one flavonol, two flavanones and three steroids, were isolated from the whole plant (roots and aerial parts) of Erinacea anthyllis. Structures of all isolated compounds were elucidated by spectroscopic analysis, including 1D and 2D NMR (1H, 13C, COSY, HSQC, TOCSY, HMBC and NOESY), mass spectrometry (ESI-MS), UV-Vis, measurement of optical rotation [α]D and by comparison with the literature data. The total phenolic and flavonoid contents were determined in this study. Furthermore, the antioxidant and antibacterial activities of the EtOAc and n-BuOH extracts of E. anthyllis were evaluated. These extracts exhibited moderate antibacterial and antioxidant activities. Their IC50 values were approximately 0.04 mg/mL ( n-BuOH) and 0.037 mg/mL (EtOAc). Consequently, Erinacea anthyllis is a rich source of polyphenolic compounds particularly isoflavonoids used as chemotaxonomic markers for the subfamily Papilionoideae of the family Fabaceae.

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Comparison of Volatile Constituents, and Antioxidant and Antibacterial Activities of the Essential Oils of Thymus caucasicus, T. kotschyanus and T. vulgaris

Natural Product Communications ◽

10.1177/1934578x1100600133 ◽

2011 ◽

Vol 6 (1) ◽

pp. 1934578X1100600 ◽

Cited By ~ 7

Author(s):

Shiva Asbaghian ◽

Ali Shafaghat ◽

Khalil Zarea ◽

Fakhraddin Kasimov ◽

Farshid Salimi

Keyword(s):

Antibacterial Activity ◽

Essential Oils ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Antibacterial Activities ◽

Dilution Method ◽

Aerial Parts ◽

Main Components ◽

Dpph Radical Scavenging ◽

Broth Dilution

A comparison of the chemical composition, and antioxidant and antibacterial activities of the essential oils obtained from the aerial parts of Thymus caucasicus, T. kotschyanus, and T. vulgaris was carried out. The oils, obtained by hydrodistillation, were analyzed by GC and GC/MS. Twenty, 29 and 22 compounds representing 94.8%, 96.6% and 98.2% of the essential oils of T. caucasicus, T. kotschyanus and T. vulgaris, respectively, have been identified. The oil of T. caucasicus was characterized by 1,8-cineol (21.5%), thymol (12.6%), β-fenchyl alcohol (8.7%), nerolidol (7.8%), terpinolene (7.2%), α-pinene (7.0%) and myrcene (6.8%). In the oil of T. kotschyanus, carvacrol (24.4%), β-caryophyllene (14.5%), γ-terpinene (12.4%), α-phellandrene (10.8%), p-cymene (9.8%) and thymol (6.8%) were the predominant compounds, whereas the main components of T. vulgaris oil were thymol (43.8%), p-cymene (15.2%), germacrene-D (11.7%), terpinolene (3.4%), carvacrol (3.2%), β-caryophyllene (2.8%) and α-thujene (2.2%). In all three plants oil, hydrocarbon monoterpenes predominated over sesquiterpenes. Antioxidant activities were assessed by determining IC50 values in the DPPH radical scavenging assay. Antibacterial activity was determined by measuring minimum inhibitory concentration (MIC) using the broth dilution method. The essential oils of T. caucasicus, T. kotschyanus and T. vulgaris showed free radical scavenging and antibacterial activity.

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