A zeta potential-based homogeneous assay for amplified detection of telomerase in cancer cells

Background: Creating a new berberine liposome with high encapsulation efficiency and slow release formulation in the treatment of cancer is a new issue. Therefore, the aim of current study was to develop slow release berberine-containing nanoliposome for delivery to bone cancer cells Saos2. Materials and Methods: In this experimental study, after synthesis nanoliposomal formulation, physical parameters, including size, zeta potential, and drug loading, in liposome were assessed using different techniques. Saos2 cell line was incubated in micro-plates containing Dulbecco's Modified Eagle's medium (DMEM) and FBS at 37˚C and 5% CO2. Cytotoxicity of nanoliposome was assessed using MTT assay. The release of drug from nanoliposome was assessed by dialysis method. P< 0.05 was assumed significant. Results: The size of drug-free nanoliposome and drug nanoliposome (berberine-nanoliposome) was 112.1 and 114.9 nm, respectively. The zeta potential of drug-free nanoliposome and drug- nanoliposome was –16.1 and –1.9 mv, respectively. There was no significant difference between control and drug-free nanoliposome groups regarding viability (p>0.05). The viability of cells in different concentration of nanoliposome containing berberines in Saos2 cell line was significantly higher than that in free berberines (p<0.05). The release of berberine at temperature 37 º C and pH 7.4 showed that approximately 47% of the drug was released in the first 12 hours of study and then the slow release of drug was continued. IC50 value of free berberine and berberine containing nanoliposome was 137.3 and 52.2 µg / ml, respectively. Conclusion: According to these findings, IC50 value of free berberine was 2.67 times more than berberine containing nanoliposome, indicating that nanoliposome containing berberine had more inhibition on growth of cancer cells than free berberine. In addition, the drug release was slow exposing the drug to the tumor for longer time at a lower dose and fewer injections, increasing the effect of the drug on cancer cells.

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Development and Characterization of Nanoliposomal Hydroxyurea Against BT-474 Breast Cancer Cells

Advanced Pharmaceutical Bulletin ◽

10.15171/apb.2020.005 ◽

2019 ◽

Vol 10 (1) ◽

pp. 39-45 ◽

Cited By ~ 2

Author(s):

Azam Akbari ◽

Azim Akbarzadeh ◽

Morteza Rafiee Tehrani ◽

Reza Ahangari Cohan ◽

Mohsen Chiani ◽

...

Keyword(s):

Breast Cancer ◽

Drug Release ◽

Zeta Potential ◽

Cancer Cells ◽

Cellular Uptake ◽

Breast Cancer Cells ◽

Drug Carriers ◽

Diffusion Method ◽

Diphenyl Tetrazolium Bromide

Purpose: Hydroxyurea (HU) is a well-known chemotherapy drug with several side effects which limit its clinical application. This study was conducted to improve its therapeutic efficiency against breast cancer using liposomes as FDA-approved drug carriers. Methods: PEGylated nanoliposomes-containing HU (NL-HU) were made via a thin-film hydration method, and assessed in terms of zeta potential, size, morphology, release, stability, cellular uptake, and cytotoxicity. The particle size and zeta potential of NL-HU were specified by zeta-sizer. The drug release from liposomes was assessed by dialysis diffusion method. Cellular uptake was evaluated by flow cytometry. The cytotoxicity was designated by methyl thiazolyl diphenyl-tetrazolium bromide (MTT) test. Results: The size and zeta value of NL-HU were gotten as 85 nm and -27 mV, respectively. NL-HU were spherical.NL-HU vesicles were detected to be stable for two months. The slow drug release and Weibull kinetic model were obtained. Liposomes considerably enhanced the uptake of HU into BT-474 human breast cancer cells. The cytotoxicity of NL-HU on BT-474 cells was found to be significantly more than that of free HU. Conclusion: The results confirmed these PEGylated nanoliposomes containing drug are potentially suitable against in vitro model of breast cancer.

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Comparative Study on Inhibition of Pancreatic Cancer Cells by Resveratrol Gold Nanoparticles and a Resveratrol Nanoemulsion Prepared from Grape Skin

Pharmaceutics ◽

10.3390/pharmaceutics13111871 ◽

2021 ◽

Vol 13 (11) ◽

pp. 1871

Author(s):

Baskaran Stephen Inbaraj ◽

Leng-Huei Hua ◽

Bing-Huei Chen

Keyword(s):

Pancreatic Cancer ◽

Particle Size ◽

Gold Nanoparticles ◽

Zeta Potential ◽

Cancer Cells ◽

Surface Plasmon Resonance Peak ◽

Grape Skin ◽

Plasmon Resonance Peak ◽

Mean Particle Size ◽

Pancreatic Cancer Cells

Resveratrol, a phenolic compound possessing vital biological activities such as anti-cancer, is present abundantly in grape skin, a waste produced during the processing of grape juice. The objectives of this study were to prepare resveratrol-gold nanoparticles and a resveratrol nanoemulsion from grape skin and study their inhibition effects on pancreatic cancer cells BxPC-3. The spherical-shaped citrate gold nanoparticles (GNPs) and resveratrol-gold nanoparticles (R-GNPs) were, respectively, prepared with a surface plasmon resonance peak at 528 and 538 nm, mean particle size of 20.8 and 11.9 nm, and zeta-potential at −32.7 and −66.7 mV, by controlling an appropriate concentration of citrate/resveratrol and gold chloride as well as stirring time and temperature. The resveratrol nanoemulsion, composed of soybean oil, Tween 80, and sucrose fatty acid ester in glycerol and water, possessed a high storage stability with a mean particle size of 14.1 nm, zeta-potential of −49.7 mV, and encapsulation efficiency of 95.5%. An antiproliferation study revealed that both R-GNPs and resveratrol nanoemulsion could effectively inhibit the growth of pancreatic cancer cells BxPC-3, with the latter showing a higher inhibition effect. Western blot analysis implied that both can down-regulate expressions of cyclin A, cyclin B, CDK1, and CDK2 and up-regulate expressions of p53 and p21, accompanied by enhancing cytochrome C expression, decreasing BcL-2 expression, increasing Bax expression, and leading to the elevation of caspase-8, caspase-9, and caspase-3 activities for cell apoptosis execution. Future research is needed to study the inhibition of pancreatic tumors in vivo by R-GNPs and resveratrol nanoemulsions.

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ConA-Coated Liposomes as a System to Delivery β-Lapachone to Breast Cancer Cells

Anti-Cancer Agents in Medicinal Chemistry ◽

10.2174/1871520621666210624112452 ◽

2021 ◽

Vol 21 ◽

Author(s):

Larissa Franca ◽

Milena Ferraz ◽

Maria Clara Barros ◽

Victor Gibson ◽

Francisco Humberto Xavier-Júnior ◽

...

Keyword(s):

Breast Cancer ◽

Zeta Potential ◽

Cancer Cells ◽

Breast Cancer Cells ◽

Encapsulation Efficiency ◽

Titration Calorimetry ◽

Specific System ◽

Site Specific ◽

Before And After ◽

Target Treatment

Background: Target treatment using site-specific nanosystems is a hot topic for treating several diseases, especially cancer. Objective: The study was set out to develop site-specific liposomes using ConcanavalinA (ConA) to target β-lapachone(β-lap) to human breast cancer cells. Methods: Liposomes were prepared and characterized according to diameter size, zeta potential, ConA conjugation(%), and β-lap encapsulation efficiency (%). Isothermal Titration Calorimetry evaluated the binding energy between the biomolecules, which compose the liposomes. ConA avidity was assessed before and after conjugation. Cytotoxicity was evaluated, and fluorescence microscopy was performed to investigate the influence of ConA influenced on MCF-7 uptake. Results: Uncoated and ConA-coated liposomes presented size, and zeta potential values from 97.46 ± 2.01 to 152.23 ± 2.73nm, and -6.83 ± 0.28 to -17.23 ±0.64mV, respectively. Both ConA conjugation and β-lap encapsulation efficiency were approximately 100%. The favorable and spontaneous process confirmed the binding between ConA and the lipid. Hemagglutination assay confirmed ConA avidity once Lipo-ConA and Lipo-PEG-ConA were able to hemagglutinate the red blood cells at 128-1 and 256-1, respectively. Lipo-ConA was not cytotoxic, and the site-specific liposomes presented the highest toxicity. ConA-coated liposomes were more internalized by MCF7 than uncoated liposomes. Conclusion: Therefore, the presence of ConA on the surface of liposomes influenced MCF7 uptake, suggesting that it could be used as a promising site-specific system to target β-lap to cancer cells.

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Anti-cancer & anti-metastasis properties of bioorganic-capped silver nanoparticles fabricated from Juniperus chinensis extract against lung cancer cells

AMB Express ◽

10.1186/s13568-021-01216-6 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Hassan Noorbazargan ◽

Sobhan Amintehrani ◽

Aghigh Dolatabadi ◽

Ainaz Mashayekhi ◽

Nazanin Khayam ◽

...

Keyword(s):

Electron Microscopy ◽

Lung Cancer ◽

Silver Nanoparticles ◽

Zeta Potential ◽

Cancer Cells ◽

Lung Cancer Cells ◽

Human Lung Cancer ◽

X Ray ◽

Juniperus Chinensis ◽

Anti Cancer

AbstractThe current study evaluated the anti-cancer properties of bio-functionalized silver nanoparticles fabricated by Juniperus chinensis leaf extracts. The nanoparticles were characterized by scanning electron microscopy, transmission electron microscopy, UV–visible spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, dynamic light scattering, Zeta potential and X-ray spectroscopy. Further, this study elucidated the cellular and molecular mechanisms of nanoparticles for anti-proliferative and apoptotic effects on human lung cancer cells (A549) and compared them with commercial drug cisplatin. The size of the spherical nanoparticle was 12.96 nm with negative zeta potential. Up-regulation of caspase 3,9 and p53, Annexin V-FITC/PI, DAPI staining, and ROS production indicated the remarkable apoptotic effect of AgNPs compared to cisplatin. Moreover, down-regulation of MMP2/MMP9 scratch and matrigel assays revealed anti-metastatic properties of AgNPs. Cell cycle analysis and downregulation of cyclin D1 indicated cancer cell cessation in the G0/G1 phase. Overall, the results revealed that the green-synthetized AgNPs had anti-metastasis and anti-proliferation effects on lung cancer cells in comparison to cisplatin with lower side effects on the normal cell line.

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PEGylated Nanographene Oxide in Combination with Near-Infrared Laser Irradiation as a Smart Nanocarrier in Colon Cancer Targeted Therapy

Pharmaceutics ◽

10.3390/pharmaceutics13030424 ◽

2021 ◽

Vol 13 (3) ◽

pp. 424

Author(s):

Milena Georgieva ◽

Zlatina Gospodinova ◽

Milena Keremidarska-Markova ◽

Trayana Kamenska ◽

Galina Gencheva ◽

...

Keyword(s):

Colon Cancer ◽

Targeted Therapy ◽

Zeta Potential ◽

Cancer Cells ◽

Near Infrared ◽

Cancer Therapies ◽

Colon Cancer Cells ◽

Biological Compatibility ◽

Fetal Bovine ◽

Cancer Targeted Therapy

Anti-cancer therapies that integrate smart nanomaterials are the focus of cancer research in recent years. Here, we present our results with PEGylated nanographene oxide particles (nGO-PEG) and have studied their combined effect with near-infrared (NIR) irradiation on low and high invasive colorectal carcinoma cells. The aim is to develop nGO-PEG as a smart nanocarrier for colon cancer-targeted therapy. For this purpose, nGO-PEG nanoparticles’ size, zeta potential, surface morphology, dispersion stability, aggregation, and sterility were determined and compared with pristine nGO nanoparticles (NPs). Our results show that PEGylation increased the particle sizes from 256.7 nm (pristine nGO) to 324.6 nm (nGO-PEG), the zeta potential from −32.9 to −21.6 mV, and wrinkled the surface of the nanosheets. Furthermore, nGO-PEG exhibited higher absorbance in the NIR region, as compared to unmodified nGO. PEGylated nGO demonstrated enhanced stability in aqueous solution, improved dispensability in the culture medium, containing 10% fetal bovine serum (FBS) and amended biocompatibility. A strong synergic effect of nGO-PEG activated with NIR irradiation for 5 min (1.5 W/cm−2 laser) was observed on cell growth inhibition of low invasive colon cancer cells (HT29) and their wound closure ability while the effect of NIR on cellular morphology was relatively weak. Our results show that PEGylation of nGO combined with NIR irradiation holds the potential for a biocompatible smart nanocarrier in colon cancer cells with enhanced physicochemical properties and higher biological compatibility. For that reason, further optimization of the irradiation process and detailed screening of nGO-PEG in combination with NIR and chemotherapeutics on the fate of the colon cancer cells is a prerequisite for highly efficient combined nanothermal and photothermal therapy for colon cancer.

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Docetaxel-Loaded Chitosan-Cholesterol Conjugate-Based Self-Assembled Nanoparticles for Overcoming Multidrug Resistance in Cancer Cells

Pharmaceutics ◽

10.3390/pharmaceutics12090783 ◽

2020 ◽

Vol 12 (9) ◽

pp. 783

Author(s):

Chao-Feng Mu ◽

Fude Cui ◽

Yong-Mei Yin ◽

Hyun-Jong Cho ◽

Dae-Duk Kim

Keyword(s):

Multidrug Resistance ◽

Zeta Potential ◽

Cancer Cells ◽

In Vitro Release ◽

Spherical Shape ◽

Entrapment Efficiency ◽

Hydrodynamic Diameter ◽

Anticancer Drug Delivery ◽

Self Assembled

Cholesteryl hemisuccinate (CHS)-conjugated chitosan (CS)-based self-assembled nanoparticles (NPs) were developed for enhancing the intracellular uptake of docetaxel in multidrug resistance (MDR)-acquired cancer cells. CHS-CS was successfully synthesized and self-aggregation, particle size, zeta potential, drug entrapment efficiency, and in vitro drug release of docetaxel-loaded CHS-CS NPs were tested. The optimized NPs had a mean hydrodynamic diameter of 303 nm, positive zeta potential of 21.3 mV, and spherical shape. The in vitro release of docetaxel from the optimized CHS-CS NPs in different pH medium (pH 6.0 and 7.4) revealed that the release was improved in a more acidic condition (pH 6.0), representing a tumor cell’s environment. The superior MDR-overcoming effect of docetaxel-loaded CHS-CS NPs, compared with docetaxel solution, was verified in anti-proliferation and cellular accumulation studies in MDR-acquired KBV20C cells. Thus, CHS-CS NPs could be potentially used for overcoming the MDR effect in anticancer drug delivery.

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Ionizing Radiation Increases the Activity of Exosomal Secretory Pathway in MCF-7 Human Breast Cancer Cells: A Possible Way to Communicate Resistance against Radiotherapy

International Journal of Molecular Sciences ◽

10.3390/ijms20153649 ◽

2019 ◽

Vol 20 (15) ◽

pp. 3649 ◽

Cited By ~ 19

Author(s):

Nasrollah Jabbari ◽

Muhammad Nawaz ◽

Jafar Rezaie

Keyword(s):

Breast Cancer ◽

Zeta Potential ◽

Cancer Cells ◽

Tumor Cells ◽

Protein Level ◽

Breast Cancer Cells ◽

Acetylcholinesterase Activity ◽

X Ray ◽

Exosome Biogenesis ◽

Mcf 7

Radiation therapy, which applies high-energy rays, to eradicate tumor cells, is considered an essential therapy for the patients with breast cancer. Most tumor cells secrete exosomes, which are involved in cell-to-cell communication in tumor tissue and contribute therapeutic resistance and promote tumor aggressiveness. Here, we investigated the effect of clinically applicable doses of X-ray irradiation (2, 4, 6, 8, 10 Gy) on the dynamics of the exosomes’ activity in MCF-7 breast cancer cells. Survival and apoptosis rate of cells against X-ray doses was examined using MTT and flow cytometry assays, respectively. Whereas, the levels of reactive oxygen species (ROS) in the X-ray-treated cells were detected by fluorometric method. The mRNA levels of vital genes involved in exosome biogenesis and secretion including Alix, Rab11, Rab27a, Rab27b, TSPA8, and CD63 were measured by real-time PCR. The protein level of CD63 was examined by Western blotting. Additionally, exosomes were characterized by monitoring acetylcholinesterase activity, transmission electron microscopy, size determination, and zeta potential. The result showed that in comparison with control group cell survival and the percentage of apoptotic cells as well as amount of ROS dose-dependently decreased and increased in irradiated cells respectively (p < 0.05). The expression level of genes including Alix, Rab27a, Rab27b, TSPA8, and CD63 as well as the protein level of CD63 upraised according to an increase in X-ray dose (p < 0.05). We found that concurrent with an increasing dose of X-ray, the acetylcholinesterase activity, size, and zeta-potential values of exosomes from irradiated cells increased (p < 0.05). Data suggest X-ray could activate exosome biogenesis and secretion in MCF-7 cells in a dose-dependent way, suggesting the therapeutic response of cells via ROS and exosome activity.

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Anti-cancer, Anti-invasiveness & Anti-metastasis of Green Engineered Bioorganic-Capped Silver Nanoparticles Fabricated from Juniperus chinensis Extract and Comparison to Cisplatin in Lung Cancer Cells (A549): In Vitro Assessment of Cellular and Molecular Pathways

10.21203/rs.3.rs-88253/v1 ◽

2020 ◽

Author(s):

Hassan Noorbazargan ◽

Ainaz Mashayekhi ◽

Nazanin Khayam ◽

Mohammad Naghizadeh ◽

Amir Mirzaie ◽

...

Keyword(s):

Electron Microscopy ◽

Lung Cancer ◽

Zeta Potential ◽

Cancer Cells ◽

Lung Cancer Cells ◽

Lower Side ◽

X Ray ◽

Juniperus Chinensis ◽

Anti Cancer ◽

Apoptotic Effect

Abstract The current study reveals anti-cancer properties of bio-functionalized silver nanoparticle (AgNPs) fabricated by Juniperus chinensis leaf extracts due to it’s easy, low cost, biological activity, eco-friendly and lower side effects. The characteristics of AgNPs were determined by scanning electron microscopy [1], transmission electron microscopy (TEM), UV-visible spectroscopy, Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction, Dynamic light scattering (DLS), Zeta potential and X-ray spectroscopy (EDX). Further, this study highlights the cellular and molecular mechanisms of AgNPs involves in anti-proliferative and apoptotic effect on human lung cancer cells (A549) and compared to commercial drug cisplatin by various biological methods such as MTT, flow cytometery analysis, gene expression patterns, migration and invasion inhibition, ROS and caspase production and cell staining. The size of AgNPs fabricated in this study was 12.96 with spherical shape and negative zeta potential. Up-regulation of capase 3,9 and p53, Annexin V-FITC/PI, DAPI staining, ROS production indicated remarkable apoptotic effect of AgNPs than cisplatin. Also down-regulation of MMP2/MMP9 scratch and matrigel assays revealed anti-metastatic properties of AgNPs.cell cycle analysis and down regulation of cyclin D1 showed cancer cell cessation in the G0/G1 phase. Overall results in current experiment revealed AgNPs synthetized by biogreen method anti-metastasis and anti-proliferation effect on lung cancer cells comparison to cicplatin drug and also had lower side effect on normal cell line.

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Preparation, Characterization and Anti-cancer Activity of Nanostructured Lipid Carriers Containing Imatinib

10.20944/preprints202105.0158.v1 ◽

2021 ◽

Author(s):

Hafiz Antar Makeen ◽

Syam Mohan ◽

Mohamed Ahmed Alkasim ◽

Muhammad Hadi Sultan ◽

Ahmed A Albarraq ◽

...

Keyword(s):

Breast Cancer ◽

Particle Size ◽

Zeta Potential ◽

Cancer Cells ◽

Breast Cancer Cells ◽

Encapsulation Efficiency ◽

Stability Studies ◽

Cytotoxicity Studies ◽

Release Studies ◽

Mcf 7

Breast cancer is the most widespread malignancy in women from corner to corner of the world. Nanostructured lipid carrier (NLC) proved its efficacy in the treatment of cancer. NLC loaded with Imatinib (IMA) (NANIMA) was prepared and evaluated for in vitro efficacy in MCF-7 breast cancer cells. Hot homogenization method was used for the preparation of NANIMAs. Aqueous solution of surfactants (hot) was mixed with molten mixture of stearic acid and sesame oil (hot) under homogenization. The prepared NANIMAs were characterized and evaluated for size, polydispersity index, zeta potential, encapsulation efficiency, release studies, stability studies and MTT assay (cytotoxicity studies). The optimized NANIMA revealed particle size of 104.63 ± 9.55 d.nm, PdI of 0.227 ± 0.06 and EE of 99.79 ± 0.03. All NANIMAs revealed slow and sustained release behaviour. The surfactants used in the preparation of NANIMAs exhibited their effects on particle size, zeta potential, encapsulation efficiency, stability studies and release studies. The cytotoxicity studies unveiled 8.75 times increase in cytotoxicity for optimized NANIMA (IC50 = 6 µM) when compared with IMA alone (IC50 = 52.5 µM) on MCF-7 breast cancer cells. After this, NLC containing IMA possibly will be employed for the cure of breast cancer. A lesser amount of IMA loaded NLC will be sufficient than IMA alone for the treatment of breast cancer. Moreover, the patient will exhibit not as much of adverse effects as in case of IMA alone. Consequently, NANIMA could prove to be useful for effective therapeutic outcome in breast cancer treatment.

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