scholarly journals A Veterinary Vaccine Development Process Map to assist in the development of new vaccines

Vaccine ◽  
2020 ◽  
Vol 38 (29) ◽  
pp. 4512-4515
Author(s):  
Michael James Francis
Keyword(s):  
Development Process ◽  
Vaccine Development ◽  
Process Map ◽  
Veterinary Vaccine ◽  
New Vaccines

scholarly journals Veterinary vaccine development: The helical project

2020 ◽  
Vol 4 (1) ◽  
pp. 042-047
Author(s):  
Dereja Iyasu Angani
Keyword(s):  
Production Process ◽  
Infection Prevention ◽  
Development Process ◽  
Vaccine Development ◽  
Vaccine Production ◽  
The Public ◽  
Health Implication ◽  
Veterinary Vaccine ◽  
Improper Use

Vaccine production process have been fuzzy journey to the public and, in some degrees, to those in the setting. By clearly showing the lengthy and challenging journey of vaccine development process, thereby suggesting the economic and health implication of improper use of veterinary vaccines, the paper tries to add the attention given to infection prevention. Starting from the foundations, the types and requirements of veterinary vaccines are described. The paper concludes with current research and regulatory quos in the topic.

scholarly journals A Novel Cre Recombinase-MediatedIn VivoMinicircle DNA (CRIM) Vaccine Provides Partial Protection against Newcastle Disease Virus

2019 ◽  
Vol 85 (14) ◽  
Author(s):  
Yanlong Jiang ◽  
Xing Gao ◽  
Ke Xu ◽  
Jianzhong Wang ◽  
Haibin Huang ◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Vaccine Development ◽  
Cre Recombinase ◽  
Attractive Alternative ◽  
Vaccine Platform ◽  
Veterinary Vaccine ◽  
Minicircle Dna

ABSTRACTMinicircle DNA (mcDNA), which contains only the necessary components for eukaryotic expression and is thus smaller than traditional plasmids, has been designed for application in genetic manipulation. In this study, we constructed a novel plasmid containing both the Cre recombinase under the phosphoglycerate kinase (PGK) promoter and recombinantlox66andlox71sites located outside the cytomegalovirus (CMV) expression cassette. The strictly controlled synthesis of Cre recombinasein vivomaintained the complete form of the plasmidin vitro, whereas thein vivoproduction of Cre transformed the parental plasmid to mcDNA after transfection. The newly designedCrerecombinase-mediatedin vivomcDNA platform, named CRIM, significantly increased the nuclear entry of mcDNA, followed by increased production of mRNA and protein, using enhanced green fluorescent protein (EGFP) as a model. Similar results were also observed in chickens when the vaccine was delivered by the regulated-delayed-lysisSalmonellastrain χ11218, where significantly increased production of EGFP was observed in chicken livers. Then, we used the HN gene of genotype VII Newcastle disease virus as an antigen model to construct the traditional plasmid pYL43 and the novel mcDNA plasmid pYL47. After immunization, our CRIM vaccine provided significantly increased protection against challenge compared with that of the traditional plasmid, providing us with a novel mcDNA vaccine platform.IMPORTANCEMinicircle DNA (mcDNA) has been considered an attractive alternative to DNA vaccines; however, the relatively high cost and complicated process of purifying mcDNA dramatically restricts the application of mcDNA in the veterinary field. We designed a novelin vivomcDNA platform in which the complete plasmid could spontaneously transform into mcDNAin vivo. In combination with the regulated-delayed-lysisSalmonellastrain, the newly designed mcDNA vaccine provides us with an elegant platform for veterinary vaccine development.

scholarly journals Antigen delivery systems for veterinary vaccine development

Vaccine ◽  
2008 ◽  
Vol 26 (51) ◽  
pp. 6508-6528 ◽  
Author(s):  
Alejandro Brun ◽  
Emmanuel Albina ◽  
Tom Barret ◽  
David A.G. Chapman ◽  
Markus Czub ◽  
...  
Keyword(s):  
Vaccine Development ◽  
Delivery Systems ◽  
Antigen Delivery ◽  
Veterinary Vaccine ◽  
Antigen Delivery Systems

scholarly journals The Importance of RNA-Based Vaccines in the Fight against COVID-19: An Overview

Vaccines ◽  
2021 ◽  
Vol 9 (11) ◽  
pp. 1345
Author(s):  
Bruna Aparecida Souza Machado ◽  
Katharine Valéria Saraiva Hodel ◽  
Larissa Moraes dos Santos Fonseca ◽  
Luís Alberto Brêda Mascarenhas ◽  
Leone Peter Correia da Silva Andrade ◽  
...  
Keyword(s):  
Messenger Rna ◽  
Vaccine Development ◽  
Research Field ◽  
Related Protein ◽  
Relevant Research ◽  
Cell Mediated Immune Response ◽  
The World ◽  
Mrna Technology ◽  
New Vaccines ◽  
Fight Against Cancer

In recent years, vaccine development using ribonucleic acid (RNA) has become the most promising and studied approach to produce safe and effective new vaccines, not only for prophylaxis but also as a treatment. The use of messenger RNA (mRNA) as an immunogenic has several advantages to vaccine development compared to other platforms, such as lower coast, the absence of cell cultures, and the possibility to combine different targets. During the COVID-19 pandemic, the use of mRNA as a vaccine became more relevant; two out of the four most widely applied vaccines against COVID-19 in the world are based on this platform. However, even though it presents advantages for vaccine application, mRNA technology faces several pivotal challenges to improve mRNA stability, delivery, and the potential to generate the related protein needed to induce a humoral- and T-cell-mediated immune response. The application of mRNA to vaccine development emerged as a powerful tool to fight against cancer and non-infectious and infectious diseases, for example, and represents a relevant research field for future decades. Based on these advantages, this review emphasizes mRNA and self-amplifying RNA (saRNA) for vaccine development, mainly to fight against COVID-19, together with the challenges related to this approach.

scholarly journals Staphylococcus aureus—A Known Opponent against Host Defense Mechanisms and Vaccine Development—Do We Still Have a Chance to Win?

2022 ◽  
Vol 23 (2) ◽  
pp. 948
Author(s):  
Urszula Wójcik-Bojek ◽  
Barbara Różalska ◽  
Beata Sadowska
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Therapy ◽  
Virulence Factors ◽  
Host Defense ◽  
Defense Mechanisms ◽  
Vaccine Development ◽  
Global Trends ◽  
Molecular Microbiology ◽  
Modern Technologies ◽  
New Vaccines

The main purpose of this review is to present justification for the urgent need to implement specific prophylaxis of invasive Staphylococcus aureus infections. We emphasize the difficulties in achieving this goal due to numerous S. aureus virulence factors important for the process of infection and the remarkable ability of these bacteria to avoid host defense mechanisms. We precede these considerations with a brief overview of the global necessitiy to intensify the use of vaccines against other pathogens as well, particularly in light of an impasse in antibiotic therapy. Finally, we point out global trends in research into modern technologies used in the field of molecular microbiology to develop new vaccines. We focus on the vaccines designed to fight the infections caused by S. aureus, which are often resistant to the majority of available therapeutic options.

scholarly journals Prediction of Physicochemical Properties of SARS-CoV-2 Protein in Bangladesh

2020 ◽  
Author(s):  
Nilanjan Roy
Keyword(s):  
Physicochemical Properties ◽  
Development Process ◽  
Vaccine Development ◽  
Viral Proteins ◽  
Instability Index ◽  
Subunit Vaccines ◽  
Grand Average ◽  
Rapid Production ◽  
Aliphatic Index ◽  
Inactivated Vaccines

Awareness of the physicochemical properties of the severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) is urgently required for the rapid production of live attenuated and inactivated vaccines. To develop subunit vaccines, it's also important to understand these properties for similar viral proteins. In this study, we predicted the physicochemical properties of SARS-CoV-2 protein that has been sequenced from Bangladeshi patients. Here we reported the number of amino acids, molecular weight, theoretical pI, amino acid composition, extinction coefficients, estimated half-life, instability index, aliphatic index, and grand average of hydropathicity of the SARS-CoV-2 protein in Bangladesh. As Bangladesh is consistently trying to contribute in the vaccine development process of SARS-CoV-2, we believe that this biocomputational study of physicochemical properties will give meaningful insights and will ease the way of vaccine development.

scholarly journals Livestock and Poultry Production in Nepal and Current Status of Vaccine Development

Vaccines ◽  
2020 ◽  
Vol 8 (2) ◽  
pp. 322
Author(s):  
Uddab Poudel ◽  
Umesh Dahal ◽  
Nabin Upadhyaya ◽  
Saroj Chaudhari ◽  
Santosh Dhakal
Keyword(s):  
Vaccine Development ◽  
Foot And Mouth Disease ◽  
Current Status ◽  
Poultry Production ◽  
Isolation And Characterization ◽  
Veterinary Vaccine ◽  
Laboratory Facilities ◽  
Commercial Poultry ◽  
The Government ◽  
The 1960S

The livestock and poultry sectors are an integral part of Nepalese economy and lifestyle. Livestock and poultry populations have continuously been increasing in the last decade in Nepal and are likely to follow that trend as the interests in this field is growing. Infectious diseases such as Foot and Mouth Disease (FMD), Peste des Petits Ruminants (PPR), hemorrhagic septicemia (HS), black quarter (BQ), swine fever, avian influenza, and Newcastle disease (ND) constitute one of the major health challenges to the Nepalese livestock and poultry industry. Vaccinations are an efficient means of preventing the occurrence and spread of several diseases in animals and birds. Considering this fact, the government of Nepal began the production of veterinary vaccines in the 1960s. Nepal is self-reliant in producing several vaccines for cattle and buffaloes, sheep and goats, pigs, and poultry. Despite these efforts, the demand for vaccines is not met, especially in the commercial poultry sector, as Nepal spends billions of rupees in vaccine imports each year. There is a need of strengthening laboratory facilities for the isolation and characterization of field strains of pathogens and capacity building for the production of different types of vaccines using the latest technologies to be self-reliant in veterinary vaccine production in the future in Nepal.

scholarly journals Quantifying the Impact of Public Perceptions on Vaccine Acceptance Using Behavioral Economics

2020 ◽  
Vol 8 ◽  
Author(s):  
Steven R. Hursh ◽  
Justin C. Strickland ◽  
Lindsay P. Schwartz ◽  
Derek D. Reed
Keyword(s):  
Behavioral Economics ◽  
Vaccine Efficacy ◽  
Development Process ◽  
Vaccine Development ◽  
Rapid Development ◽  
Public Perceptions ◽  
Mixed Effect ◽  
Behavioral Economic ◽  
Vaccine Acceptance ◽  
The Impact

This study was conducted to evaluate the impact of public perceptions of vaccine safety and efficacy on intent to seek COVID-19 vaccination using hypothetical vaccine acceptance scenarios. The behavioral economic methodology could be used to inform future public health vaccination campaigns designed to influence public perceptions and improve public acceptance of the vaccine. In June 2020, 534 respondents completed online validated behavioral economic procedures adapted to evaluate COVID-19 vaccine demand in relation to a hypothetical development process and efficacy. An exponential demand function was used to describe the proportion of participants accepting the vaccine at each efficacy. Linear mixed effect models evaluated development process and individual characteristic effects on minimum required vaccine efficacy required for vaccine acceptance. The rapid development process scenario increased the rate of decline in acceptance with reductions in efficacy. At 50% efficacy, 68.8% of respondents would seek the standard vaccine, and 58.8% would seek the rapid developed vaccine. Rapid vaccine development increased the minimum required efficacy for vaccine acceptance by over 9 percentage points, γ = 9.36, p < 0.001. Past-3-year flu vaccination, γ = −23.00, p < 0.001, and male respondents, γ = −4.98, p = 0.037, accepted lower efficacy. Respondents reporting greater conspiracy beliefs, γ = 0.39, p < 0.001, and political conservatism, γ = 0.32, p < 0.001, required higher efficacy. Male, γ = −4.43, p = 0.013, and more conservative, γ = −0.09, p = 0.039, respondents showed smaller changes in minimum required efficacy by development process. Information on the vaccine development process, vaccine efficacy, and individual differences impact the proportion of respondents reporting COVID-19 vaccination intentions. Behavioral economics provides an empirical method to estimate vaccine demand to target subpopulations resistant to vaccination.

Sign in / Sign up

Export Citation Format

Share Document