Influence of cooling rate on Saccharomyces cerevisiae destruction during freezing: unexpected viability at ultra-rapid cooling rates

Cryobiology ◽  
2003 ◽  
Vol 46 (1) ◽  
pp. 33-42 ◽  
Author(s):  
Frédéric Dumont ◽  
Pierre-André Marechal ◽  
Patrick Gervais
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cooling Rate ◽  
Cooling Rates ◽  
Rapid Cooling

Boar semen can tolerate rapid cooling rates prior to freezing

2011 ◽  
Vol 23 (5) ◽  
pp. 681 ◽  
Author(s):  
Jorge D. Juarez ◽  
Inma Parrilla ◽  
Juan M. Vazquez ◽  
Emilio A. Martinez ◽  
Jordi Roca
Keyword(s):  
Cooling Rate ◽  
Flow Cytometric Analysis ◽  
Membrane Integrity ◽  
Annexin V ◽  
Objective Evaluation ◽  
Membrane Lipid ◽  
Cooling Rates ◽  
Rapid Cooling ◽  
Motile Cells ◽  
Boar Spermatozoa

Two experiments were performed in the present study that demonstrated that boar spermatozoa are capable of surviving rapid cooling rates within a range of 15–5°C before freezing. Boar ejaculates diluted in Beltsville thawing solution (BTS) (1 : 1, v/v) were held at 17–20°C and shipped over a 24-h time period from two AI centres to a cryobiology laboratory, where they were pooled (Experiment 1) or cryopreserved individually (Experiment 2) using a standard 0.5-mL straw freezing protocol. The effects of cooling before freezing were assessed after thawing through the objective evaluation of sperm motility and flow cytometric analysis of membrane integrity, acrosomal status, changes in membrane lipid architecture monitored by merocyanine and annexin V binding and intracellular production of reactive oxygen species. In Experiment 1 (six replicates), two semen pools (five ejaculates per pool) were cooled from 15 to 5°C at rates of 0.08, 0.13, 0.40 and 1.50°C min–1. These cooling rates did not result in any significant differences (P > 0.05) in any of the post-thaw sperm assessments, even in thawed samples incubated under capacitation conditions. In Experiment 2, three individual ejaculates from 16 boars were slowly (0.08°C min–1) or rapidly (1.5°C min–1) cooled before freezing. A consistent interboar variability (P < 0.01) was detected, which was independent of the cooling rate used. Cooling rate only significantly influenced (P < 0.05) sperm assessments in four of 16 boars, which exhibited slightly higher percentages of motile cells and intact plasma and acrosomal membranes in the samples that had been cooled slowly. These findings demonstrate that boar spermatozoa undergoing cryopreservation can withstand rapid cooling rates before freezing.

scholarly journals Vitrifying multiple embryos in different arrangements does not alter the cooling rate

2021 ◽  
Author(s):  
Timothy Ostler ◽  
Thomas E Woolley ◽  
Karl Swann ◽  
Andrew Thomson ◽  
Giles Palmer ◽  
...  
Keyword(s):  
Mathematical Model ◽  
Cooling Rate ◽  
Survival Rates ◽  
Spatial Arrangement ◽  
Common Method ◽  
Slow Freezing ◽  
Cooling Rates ◽  
Rapid Cooling ◽  
Rate Of Cooling ◽  
Over Time

Vitrification is the most common method of cryopreservation of gametes in fertility clinics due to its improved survival rates compared to slow freezing techniques. For the Open Cryotop® vitrification device, the number of oocytes, or embryos, mounted onto a single device can vary. In this work, a mathematical model is developed for the cooling of oocytes, or embryos, that is solved computationally, to investigate whether varying the number of samples mounted onto the Open Cryotop® affects the cooling rates, and therefore survival rates, of vitrified samples. Several realistic spatial arrangements of oocytes/embryos are examined, determining the temperature of the system over time, which highlights the effect of spatial arrangement on the rate of cooling. Results indicate that neither spatial arrangement nor the number of mounted oocytes, or embryos, has a large effect on cooling rates, so long as the volume of the cryoprotectant remains minimal. Under the manufacturer's guidelines, clinical decisions regarding the number and arrangement of oocytes or embryos placed on a device can be varied, whilst maintaining rapid cooling.

The effect of cooling rate on crystallization behavior and tensile properties of CF/PEEK composites

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Guangming Dai ◽  
Lihua Zhan ◽  
Chenglong Guan ◽  
Minghui Huang
Keyword(s):  
Cooling Rate ◽  
Crystallization Temperature ◽  
Crystallization Behavior ◽  
Crystalline Polymer ◽  
Scanning Calorimetry ◽  
Nonisothermal Crystallization ◽  
Cooling Rates ◽  
Control Range ◽  
Temperature Range ◽  
Transverse Tensile

Abstract In this study, the differential scanning calorimetry (DSC) tests were performed to measure the nonisothermal crystallization behavior of carbon fiber reinforced polyether ether ketone (CF/PEEK) composites under different cooling rates. The characteristic parameters of crystallization were obtained, and the nonisothermal crystallization model was established. The crystallization temperature range of the material at different cooling rates was predicted by the model. The unidirectional laminates were fabricated at different cooling rates in the crystallization temperature range. The results showed that the crystallization temperature range shifted to a lower temperature with the increase of cooling rate, the established nonisothermal crystallization model was consistent with the DSC test results. It is feasible to shorten the cooling control range from the whole process to the crystallization range. The crystallinity and transverse tensile strength declined significantly with the increase of the cooling rate in the crystallization temperature range. The research results provided theoretical support for the selection of cooling conditions and temperature control range, which could be applied to the thermoforming process of semi-crystalline polymer matrixed composites to improve the manufacturing efficiency.

Effects of RE on Critical Cooling Rate of Bearing-B Steel

2012 ◽  
Vol 535-537 ◽  
pp. 761-763 ◽  
Author(s):  
Yi Sheng Zhao ◽  
Xin Ming Zhang ◽  
Zhi Guo Gao
Keyword(s):  
Phase Change ◽  
Cooling Rate ◽  
Experimental Results ◽  
Cooling Rates ◽  
Critical Cooling Rate ◽  
The Law

The law of phase change of bearing-B steel during continual cooling was studied by adopting dilatometer. The CCT curves of bearing-B steel were drawn, and the effects of RE on critical cooling rates were studied. The experimental results show that the start temperatures of martensite TM was decreased from 438 to 404°C. The critical cooling rate was simultaneously decreased from 33 to 15°C/s.

Effects of the Cooling Rate on the Acoustic Properties of Pb-10Sn Solder

2012 ◽  
Vol 729 ◽  
pp. 356-360
Author(s):  
Endre Harkai ◽  
Tamás Hurtony ◽  
Péter Gordon
Keyword(s):  
Sound Velocity ◽  
Cooling Rate ◽  
Acoustic Microscopy ◽  
Acoustic Properties ◽  
Scanning Acoustic Microscopy ◽  
Cooling Rates ◽  
Reliability Parameters ◽  
Microscopy Characterization ◽  
The Given

Microhardness and sound velocity were measured in case of differently prepared solder samples. The used Pb-10Sn solder samples were melted then cooled down applying different cooling rates. These procedures caused variant microstructure thus different microhardness and sound velocity values. The sound velocity was measured by means of scanning acoustic microscopy. Characterization of solder materials by acoustic microscopy gives the possibility to non-destructively estimate mechanical and reliability parameters of the given material.

The effect of various cooling rates on the membrane ultrastructure of frozen human erythrocytes and its relation to the extent of haemolysis after thawing

1981 ◽  
Vol 49 (1) ◽  
pp. 369-382
Author(s):  
S. Fujikawa
Keyword(s):  
Cooling Rate ◽  
Human Erythrocytes ◽  
Ice Crystals ◽  
Erythrocyte Membranes ◽  
Ultrastructural Changes ◽  
Cooling Rates ◽  
Intracellular Ice ◽  
Frozen State ◽  
Membrane Ultrastructure ◽  
Fast Freezing

Human erythrocytes suspended in buffered isotonic saline were frozen to the temperature of liquid nitrogen at various cooling rates of 3, 140, 700, 1800, 3500, 8000 and 11 500 deg. C/min. The membrane ultrastructure in the frozen state and the extent of haemolysis after thawing were examined at each cooling rate. As the cooling rates increased from 3 to 3500 deg. C/min, the extent of lysis gradually decreased, but further increase in cooling rates in excess of 8000 deg. C/min resulted in an abrupt increase of lysis. Membrane-associated vesicles devoid of intramembrane particles (IMPs) were formed in the erythrocyte membranes frozen at cooling rates slower than 1800 deg. C/min. The frequency and size of these vesicles were highly cooling-rate-dependent and they were no longer formed in the erythrocyte membranes frozen at cooling rates faster than 3500 deg. C/min. Another membrane ultrastructural change associated closely with the formation of intracellular ice crystals appeared at cooling rates faster than 8000 deg. C/min. The membrane regions in direct contact with intracellular ice crystals were physically damaged and had an appearance resembling worm-eaten spots. The erythrocytes frozen at a cooling rate of 3500 deg. C/min exhibited ultrastructural integrity of the membrane by avoiding the membrane changes caused by either slow or fast freezing. It is suggested, from the close relation between membrane ultrastructure and the extent of haemolysis, that the ultrastructural integrity of membrane in the frozen state is important for avoiding haemolysis after thawing, and that the membrane ultrastructural changes caused by both slow and fast freezing were responsible for the lysis after thawing.

Phase Selection in Sub-Rapidly Solidified Au-20Sn Alloys

2015 ◽  
Vol 817 ◽  
pp. 325-330
Author(s):  
Yu Hai Qu ◽  
Kai Jin Yang ◽  
Yan Tian Zhou ◽  
Yong Mao ◽  
Wei Zhang ◽  
...  
Keyword(s):  
Cooling Rate ◽  
Nucleation Theory ◽  
Classical Nucleation Theory ◽  
Copper Mold ◽  
Cooling Rates ◽  
Phase Selection ◽  
Graphite Mold ◽  
Injection Casting ◽  
Rapidly Solidified ◽  
Ausn Phase

The sub-rapidly solidified Au-20Sn eutectic alloys were prepared by four different solidification pathways, such as, graphite mold conventional casting, graphite mold injection casting, copper mold injection casting, and water-cooled copper mold suction casting. The precipitating sequences of competing primary phases of sub-rapidly solidified Au-20Sn alloys with four different cooling rates were investigated. The results show that phase selection process is related to the cooling rates during sub-rapid solidification process. The primary ζ'-Au5Sn phase with developed dendrites precipitate at low cooling rate (2.4×10−4.2×102K/min) and the morphologies of the primary ζ'-Au5Sn change to rosette-like at higher cooling rate (9.0×103K/min). While the cooling rate reaches to 3.5×104K/min, the primary ζ'-Au5Sn phase can be suppressed but δ-AuSn phase will precipitate prior to the ζ'-Au5Sn phase. On the basis of the classical nucleation theory and transient nucleation theory, the process of competitive nucleation between the ζ'-Au5Sn phase and the δ-AuSn phase were analyzed for sub-rapid solidified Au-20Sn alloy. The theoretical calculations are consistent with the experimental investigations.

scholarly journals Analyses of Nocturnal Temperature Cooling-Rate Response to Historical Local-Scale Urban Land-Use/Land Cover Change

2011 ◽  
Vol 50 (9) ◽  
pp. 1872-1883 ◽  
Author(s):  
Winston T. L. Chow ◽  
Bohumil M. Svoma
Keyword(s):  
Land Use ◽  
Land Cover ◽  
Cooling Rate ◽  
Rural Areas ◽  
Urban Areas ◽  
Local Scale ◽  
Urban Land Use ◽  
Cooling Rates ◽  
Near Surface ◽  
Over Time

AbstractUrbanization affects near-surface climates by increasing city temperatures relative to rural temperatures [i.e., the urban heat island (UHI) effect]. This effect is usually measured as the relative temperature difference between urban areas and a rural location. Use of this measure is potentially problematic, however, mainly because of unclear “rural” definitions across different cities. An alternative metric is proposed—surface temperature cooling/warming rates—that directly measures how variations in land-use and land cover (LULC) affect temperatures for a specific urban area. In this study, the impact of local-scale (<1 km2), historical LULC change was examined on near-surface nocturnal meteorological station temperatures sited within metropolitan Phoenix, Arizona, for 1) urban versus rural areas, 2) areas that underwent rural-to-urban transition over a 20-yr period, and 3) different seasons. Temperature data were analyzed during ideal synoptic conditions of clear and calm weather that do not inhibit surface cooling and that also qualified with respect to measured near-surface wind impacts. Results indicated that 1) urban areas generally observed lower cooling-rate magnitudes than did rural areas, 2) urbanization significantly reduced cooling rates over time, and 3) mean cooling-rate magnitudes were typically larger in summer than in winter. Significant variations in mean nocturnal urban wind speeds were also observed over time, suggesting a possible UHI-induced circulation system that may have influenced local-scale station cooling rates.

Study of the Volume Deformation and Stress Development of Concrete Influenced by the Different Cooling Rate

2015 ◽  
Vol 744-746 ◽  
pp. 1495-1498
Author(s):  
Yi Fang ◽  
Xue Feng Song ◽  
Long Chen
Keyword(s):  
Thermal Stress ◽  
Cooling Rate ◽  
Temperature Control ◽  
Control Measures ◽  
Stress And Strain ◽  
Stress Tests ◽  
Volume Deformation ◽  
Cooling Rates ◽  
Stress Development ◽  
Concrete Placement

This paper studied the development of stress and strain influences by different cooling rates based on the concrete thermal stress tests. furthermore, discussed the temperature control measures for early concrete placement.

scholarly journals Development of Precipitation Hardening Parameters for High Strength Alloy AA 7068

Materials ◽  
2020 ◽  
Vol 13 (4) ◽  
pp. 918
Author(s):  
Julia Osten ◽  
Benjamin Milkereit ◽  
Michael Reich ◽  
Bin Yang ◽  
Armin Springer ◽  
...  
Keyword(s):  
Heat Treatment ◽  
Cooling Rate ◽  
Maximum Yield ◽  
High Strength ◽  
Tensile Tests ◽  
Age Hardening ◽  
Scanning Calorimetry ◽  
Cooling Rates ◽  
Critical Cooling Rate ◽  
Kinetics Of

The mechanical properties after age hardening heat treatment and the kinetics of related phase transformations of high strength AlZnMgCu alloy AA 7068 were investigated. The experimental work includes differential scanning calorimetry (DSC), differential fast scanning calorimetry (DFSC), sophisticated differential dilatometry (DIL), scanning electron microscopy (SEM), as well as hardness and tensile tests. For the kinetic analysis of quench induced precipitation by dilatometry new metrological methods and evaluation procedures were established. Using DSC, dissolution behaviour during heating to solution annealing temperature was investigated. These experiments allowed for identification of the appropriate temperature and duration for the solution heat treatment. Continuous cooling experiments in DSC, DFSC, and DIL determined the kinetics of quench induced precipitation. DSC and DIL revealed several overlapping precipitation reactions. The critical cooling rate for a complete supersaturation of the solid solution has been identified to be 600 to 800 K/s. At slightly subcritical cooling rates quench induced precipitation results in a direct hardening effect resulting in a technological critical cooling rate of about 100 K/s, i.e., the hardness after ageing reaches a saturation level for cooling rates faster than 100 K/s. Maximum yield strength of above 600 MPa and tensile strength of up to 650 MPa were attained.

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