Superoxide production in DLD-1 and HT-29 cell lines and in primary epithelial cells from normal human colon

2000 ◽  
Vol 118 (4) ◽  
pp. A98
Author(s):  
Anders Perner ◽  
Lars Andresen ◽  
Gitte Pedersen ◽  
Torben Saermark ◽  
Jorn Brynskov ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Cell Lines ◽  
Human Colon ◽  
Superoxide Production ◽  
Normal Human ◽  
Ht 29

Normal human colon epithelial cells propagated in long-term cell cultures support JC polyomavirus infection

2001 ◽  
Vol 33 ◽  
pp. A40
Author(s):  
Farhad F. Shadan ◽  
Luigi Ricciardiello ◽  
Ajay Goel ◽  
Wendy Smith ◽  
Dong K. Chang ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Cell Cultures ◽  
Human Colon ◽  
Jc Polyomavirus ◽  
Normal Human

scholarly journals Phenolic Fractions from Vaccinium vitis-idaea L. and Their Antioxidant and Anticancer Activities Assessment

Antioxidants ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 1261
Author(s):  
Gabriele Vilkickyte ◽  
Lina Raudone ◽  
Vilma Petrikaite
Keyword(s):  
Antioxidant Activity ◽  
Biological Activity ◽  
Cell Lines ◽  
Radical Scavenging ◽  
Colon Adenocarcinoma ◽  
Human Colon ◽  
Anticancer Activities ◽  
Cell Renal Cell Carcinoma ◽  
Human Malignant Melanoma ◽  
Ht 29

Lingonberry leaves and fruits are associated with a range of potential bioactivities related to their phenolic content and composition, but the identification of major biological activity markers remains limited. The present study aimed at the isolation of lingonberry phenolic fractions and biological activity evaluation of them. Crude dry extracts of lingonberry leaves and fruits were fractionated by chromatography using Sephadex LH-20 and analyzed by validated HPLC-PDA method. For each fraction, the anticancer activity against human clear cell renal cell carcinoma (CaKi-1), human colon adenocarcinoma (HT-29), and human malignant melanoma (IGR39) cell lines was determined using MTT assay, and the radical scavenging, reducing, and chelating activities were investigated using ABTS, FRAP, and FIC assays, respectively. Further, 28 phenolics were identified and quantified in the crude extract of lingonberry leaves and 37 in the extract of fruits. These compounds, during fractionation steps, were selectively eluted into active fractions, enriched with different groups of phenolics—monophenols, anthocyanins, phenolic acids, catechins, flavonols, or proanthocyanidins. Fractions of lingonberry leaves and fruits, obtained by the last fractionation step, proved to be the most active against tested cancer cell lines and possessed the greatest antioxidant activity. In this perspective, the predominant compounds of these fractions—polymeric and mainly A-type dimeric proanthocyanidins—also quercetin can be considered to be anticancer and antioxidant activity markers of lingonberries.

TNF-α-mediated apoptosis in normal human prostate epithelial cells and tumor cell lines

2004 ◽  
Vol 203 (2) ◽  
pp. 145-154 ◽  
Author(s):  
Dharam P. Chopra ◽  
Raymond E. Menard ◽  
Jakub Januszewski ◽  
Raymond R. Mattingly
Keyword(s):  
Epithelial Cells ◽  
Tumor Cell ◽  
Cell Lines ◽  
Human Prostate ◽  
Tumor Cell Lines ◽  
Prostate Epithelial Cells ◽  
Tnf Α ◽  
Normal Human

scholarly journals Down-regulation of aquaporin3 expression by lipopolysaccharide via p38/c-Jun N-terminal kinase signalling pathway in HT-29 human colon epithelial cells

2015 ◽  
Vol 21 (15) ◽  
pp. 4547-4554 ◽  
Author(s):  
Feng-Xia Li ◽  
Li-Zhen Huang ◽  
Chuan Dong ◽  
Jun-Ping Wang ◽  
Hong-Juan Wu ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Human Colon ◽  
Signalling Pathway ◽  
Down Regulation ◽  
Ht 29

Molecular cloning and promoter analysis of downregulated in adenoma (DRA)

2007 ◽  
Vol 293 (5) ◽  
pp. G923-G934 ◽  
Author(s):  
Waddah A. Alrefai ◽  
Xiaoming Wen ◽  
Wen Jiang ◽  
Jonathan P. Katz ◽  
Kris A. Steinbrecher ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Epithelial Cells ◽  
Cell Lines ◽  
Intestinal Inflammation ◽  
Human Growth ◽  
Human Colon ◽  
Promoter Fragment ◽  
Anion Transporter ◽  
Gata Transcription Factors ◽  
Ifn Γ

Downregulated in adenoma (DRA), also referred to as SLC26A3, is an intestinal anion transporter essential for intestinal chloride absorption. Mutations in DRA result in congenital chloride diarrhea. DRA expression has been shown to be induced by differentiation and to be modulated by cytokines. However, mechanisms of DRA gene transcription and its tissue-specific targeting have not yet been investigated. In this study, we cloned a 3,765-bp promoter fragment of human DRA gene and characterized its activity in human colonic LS174T and Caco-2 human colon cell lines. Primer extension identified a single transcriptional initiation site that was identical in both colon cancer cell lines and normal colon. Although hepatic nuclear factor HNF-4 is involved in the basal activity of DRA promoter, sodium butyrate induces its activity in LS174T cells via the binding of Yin Yang 1 (YY1) and GATA transcription factors to their respective cis-elements in promoter region. We also demonstrated a reduction in DRA promoter activity in Caco-2 cells by IFN-γ, suggesting that regulation of DRA promoter by IFN-γ may contribute to the pathophysiology of intestinal inflammation. Furthermore, we showed that the DRA promoter fragment is sufficient to drive human growth hormone transgene expression specifically in villus epithelial cells of the small intestine and in differentiated upper crypt and surface epithelial cells of the colon. Our studies provide evidence for the involvement of HNF-4, YY1, and GATA transcription factors in DRA expression in intestinal differentiated epithelial cells.

scholarly journals Celecoxib increases retinoid sensitivity in human colon cancer cell lines

2010 ◽  
Vol 15 (3) ◽  
Author(s):  
Jian-Pei Liu ◽  
Hong-Bo Wei ◽  
Zong-Heng Zheng ◽  
Wei-Ping Guo ◽  
Jia-Feng Fang
Keyword(s):  
Colon Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Human Colon ◽  
Colon Cancer Cell ◽  
Human Colon Cancer Cell ◽  
Human Colon Cancer ◽  
Colon Cancer Cell Lines ◽  
Cox 2 ◽  
Ht 29

AbstractRetinoid resistance has limited the clinical application of retinoids as differentiation-inducing and apoptosis-inducing drugs. This study was designed to investigate whether celecoxib, a selective COX-2 inhibitor, has effects on retinoid sensitivity in human colon cancer cell lines, and to determine the possible mechanism of said effects. Cell viability was measured using the MTT assay. Apoptosis was detected via Annexin-V/PI staining and the flow cytometry assay. PGE2 production was measured with the ELISA assay. The expression of RARβ was assayed via western blotting. The results showed that celecoxib enhanced the inhibitory effect of ATRA in both COX-2 high-expressing HT-29 and COX-2 low-expressing SW480 cell lines. Further study showed the ATRA and celecoxib combination induced greater apoptosis, but that the addition of PGE2 did not affect the enhanced growth-inhibitory and apoptosis-inducing effects of the combination. Moreover, NS398 (another selective COX-2 inhibitor) did not affect the inhibitory effects of ATRA in the two cell lines. Western blotting showed that the expression of RARβ in HT-29 cell lines was increased by celecoxib, but not by NS398, and that the addition of PGE2 did not affect the celecoxib-induced expression of the retinoic acid receptor beta. In conclusion, celecoxib increased the expression of RARβ and the level of cellular ATRA sensitivity through COX-2-independent mechanisms. This finding may provide a potential strategy for combination therapy.

scholarly journals Synthesis and Biological Evaluation of Novel Aminochalcones as Potential Anticancer and Antimicrobial Agents

Molecules ◽  
2019 ◽  
Vol 24 (22) ◽  
pp. 4129 ◽  
Author(s):  
Joanna Kozłowska ◽  
Bartłomiej Potaniec ◽  
Dagmara Baczyńska ◽  
Barbara Żarowska ◽  
Mirosław Anioł
Keyword(s):  
Antimicrobial Activity ◽  
Cell Lines ◽  
Antimicrobial Agents ◽  
Human Colon ◽  
Biological Properties ◽  
Biological Evaluation ◽  
Human Colon Cancer ◽  
E Coli ◽  
Almost All ◽  
Ht 29

A series of 18 aminochalcone derivatives were obtained in yields of 21.5–88.6% by applying the classical Claisen-Schmidt reaction. Compounds 4–9, 14 and 16–18 with 4-ethyl, 4-carboxy-, 4-benzyloxy- and 4-benzyloxy-3-methoxy groups were novel, not previously described in the scientific literature. To determine the biological properties of the synthesized compounds, anticancer and antimicrobial activity assays were performed. Antiproliferative potential was evaluated on four different human colon cancer cell lines—HT-29, LS180, LoVo and LoVo/DX —using the SRB assay and compared with green monkey kidney fibroblasts COS7. Anticancer activity was described as the IC50 value. The best results were observed for 2′-aminochalcone (1), 3′-aminochalcone (2) and 4′-aminochalcone (3) (IC50 = 1.43–1.98 µg·mL−1) against the HT-29 cell line and for amino-nitrochalcones 10–12 (IC50 = 2.77–3.42 µg·mL−1) against the LoVo and LoVo/DX cell lines. Moreover, the antimicrobial activity of all derivatives was evaluated on two strains of bacteria: Escherichia coli ATCC10536 and Staphylococcus aureus DSM799, the yeast strain Candida albicans DSM1386 and three strains of fungi: Alternaria alternata CBS1526, Fusarium linii KB-F1 and Aspergillus niger DSM1957. In the case of E. coli ATCC10536 almost all derivatives hindered the bacterial growth (∆OD = 0). Furthermore, the best results were observed in the presence of 4′-aminochalcone (3), that completely limited the growth of all tested strains at the concentration range of 0.25–0.5 mg·mL−1. The strongest bacteriostatic activity was exhibited by novel 3′-amino-4-benzyloxychalcone (14), that prevented the growth of E. coli ATCC10536 with MIC = 0.0625 mg·mL−1.

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