Production of γ-decalactone from ricinoleic acid by immobilized cells of Sporidiobolus salmonicolor

Castor oil (Ricinus communus L.) is an important commercial product. The climatic conditions of Ukraine determine the possibility of growing the castor as an annual crop. At the Institute of Oilseeds NAAS studied castor collection. The aim of the work was the selection of the most promising samples of castor oil, combining a large yield potential in a narrow range of vertical distribution for optimal technological parameters of mechanical harvesting with a high content of oil in seeds and ricinolic acid in oil. In the experience of 2015-2016, the manifestation of morphological features of 17 castor bean samples was studied. The height of plants, individual samples among themselves differed more than twice. Long-brush samples of ЕР118, К374, М203, К159 are distinguished on the basis of the length of the brush. The shortest brush was observed in sample K1008. The length of the productive brush in the studied samples is from 10.7 to 32.9 cm. Most castor bean samples under favorable conditions form brushes of the second and higher orders. According to this parameter, samples of Ep118 and selection No. 38 with four inflorescences of the second order are of the greatest interest. The largest brushes of the second order are similar in size to the brushes of the first order were observed in the samples: К1127, К810, К153. The adaptability of harvesting castor beads requires that the brushes of the first and second order coincide in height with each other, since the harvester can take a maximum of 60 cm. For the sum of the productive brushes of the first and second orders, the greatest potential yield will be provided by samples K159 and K1127. Among the studied collection stands out the small seed sample K159 and the large seed samples - PRL41 and K80. The average oil content in the seeds of the collection was from 52 to 61.4%. Sample38 had the highest oil content. The content of ricinolic acid in the collection was from 70.9 to 82.9%. Samples were isolated: К134, К1008, PRL41, К430 with the content of ricinoleic acid more than 80%. The results of the study of all parameters make it possible to isolate valuable technological samples. Sample K1064 with a high technological potential of productivity, with a seed oil content of 57.2%, has a not very high content of ricinoleic acid of 74.3%. Sample K1127 with an oil content of 58.6%, a mass of 1000 seeds of 265 g, a high potential of productive brushes has a wide variation in the arrangement of brushes. Sample K134 with a oil content of 57.1%, ricinoleic acid content of 80.7% has small second-order brushes and can be used as a single-cysts in a thicker seeding.

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Chemical composition of Azadirachta indica A. Juss and Ricinus communis Linn. seed oils growing in Marigat, Baringo County, Kenya

East African Journal of Science, Technology and Innovation ◽

10.37425/eajsti.1.2.88 ◽

2020 ◽

Vol 1 (2) ◽

Author(s):

Ann Kiplagat Jepkorir ◽

Charles Maina Irungu ◽

Philip Bett Kendagor

Keyword(s):

Chemical Composition ◽

Ricinoleic Acid ◽

Unsaturated Fatty Acids ◽

Ricinus Communis ◽

Methyl Esters ◽

Octadecenoic Acid ◽

Seed Oils ◽

Octadecanoic Acid ◽

Alkyd Resins ◽

Natural Remedies

All parts of A. indica (neem) and R. communis (castor) plants have mostly been used as natural remedies in the control and treatment of several ailments, control of pests and insects, animal feeds and production of industrial products globally. The seed oils of A. indica and R. communis are known to have antidiabetic, anti-helminthic, antifertility, antioxidant, antibacterial, anti-inflammatory, anti-cancer, insecticidal and mosquitocidal activity. This study reports for the first time the chemical composition of A. indica and R. communis seed oils from Marigat, Baringo County, Kenya. Seed oils of A. indica and R. communis were extracted from mature dried seeds through cold pressing and boiling respectively and chemical composition determined using Gas Chromatography (GC)-Mass Spectrometry (MS). The constituents of both seed oils were dominated by saturated and unsaturated fatty acids, cyclic esters and methyl esters. The predominant constituents of R. communis were (Z)-6-Octadecenoic acid (37.33%), Ricinoleic acid (30.22%) and 13-Hexyloxacyclotridec-10-en-2-one (26.67%) while those of A. indica were 2-hexyl-1-decanol (30.97%), Octadecanoic acid (29.69%) and Oxalic acid, 6-ethyloct-3-yl ethyl ester (15.55%). Oils contained Hexadecanoic acid and Octadecanoic acid which are used in the manufacture of several products such as candles, soaps, lotions, perfumes and cosmetics. Octadecenoic acid is important in control of human diseases and Ricinoleic acid in production of alkyd resins for surface coating and biofuel. From the results, A. indica and R. communis seed oils constituents have potential in the agricultural, industrial, comestics and pharmaceutical sectors but require further fractionation to isolate the bioactive compounds.

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Fermentation of Groundnut Shell Enzymatic Hydrolysate for Fuel Ethanol Production by Free and Sorghum Stalks Immobilized Cells of Pichia stipitis NCIM 3498

International Journal of Chemical Reactor Engineering ◽

10.2202/1542-6580.2514 ◽

2011 ◽

Vol 9 (1) ◽

Author(s):

Chandrasekhar Gajula ◽

Anuj Kumar Chandel ◽

Radhika Konakalla ◽

Ravinder Rudravaram ◽

Ravindra Pogaku ◽

...

Keyword(s):

Ethanol Production ◽

Immobilized Cells ◽

Pichia Stipitis ◽

Fuel Ethanol ◽

Enzymatic Hydrolysate ◽

Groundnut Shell

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Design of a continuous flow immobilized-cell reactor for biosynthetical production of L-aspartic acid

Collection of Czechoslovak Chemical Communications ◽

10.1135/cccc19852122 ◽

1985 ◽

Vol 50 (10) ◽

pp. 2122-2133 ◽

Cited By ~ 2

Author(s):

Jindřich Zahradník ◽

Marie Fialová ◽

Jan Škoda ◽

Helena Škodová

Keyword(s):

Aspartic Acid ◽

Continuous Flow ◽

Immobilized Cells ◽

Scale Up ◽

Fixed Bed ◽

Packed Bed ◽

Fixed Bed Reactor ◽

Experimental Program ◽

Design Parameters ◽

Immobilized Cell

An experimental study was carried out aimed at establishing a data base for an optimum design of a continuous flow fixed-bed reactor for biotransformation of ammonium fumarate to L-aspartic acid catalyzed by immobilized cells of the strain Escherichia alcalescens dispar group. The experimental program included studies of the effect of reactor geometry, catalytic particle size, and packed bed arrangement on reactor hydrodynamics and on the rate of substrate conversion. An expression for the effective reaction rate was derived including the effect of mass transfer and conditions of the safe conversion-data scale-up were defined. Suggestions for the design of a pilot plant reactor (100 t/year) were formulated and decisive design parameters of such reactor were estimated for several variants of problem formulation.

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Biological removal of benzalkonium chlorides from wastewater by immobilized cells of Pseudomonas sp. BIOMIG1 in an up-flow packed bed reactor

Journal of Hazardous Materials ◽

10.1016/j.jhazmat.2021.126210 ◽

2021 ◽

pp. 126210

Author(s):

Fahri Koray Sakarya ◽

Berat Zeki Haznedaroglu ◽

Ulas Tezel

Keyword(s):

Immobilized Cells ◽

Packed Bed ◽

Packed Bed Reactor ◽

Pseudomonas Sp ◽

Biological Removal

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Mead Production Using Immobilized Cells of Saccharomyces cerevisiae: Reuse of Sodium Alginate Beads

Processes ◽

10.3390/pr9040724 ◽

2021 ◽

Vol 9 (4) ◽

pp. 724

Author(s):

Miguel L. Sousa-Dias ◽

Vanessa Branco Paula ◽

Luís G. Dias ◽

Letícia M. Estevinho

Keyword(s):

Saccharomyces Cerevisiae ◽

Sodium Alginate ◽

Fermentation Process ◽

Immobilized Cells ◽

Alginate Beads ◽

Production Medium ◽

Alcohol Content ◽

Volatile Acidity ◽

The Matrix ◽

Second Category

This work studied the production of mead using second category honey and the immobilized cells of Saccharomyces cerevisiae in sodium alginate, with concentrations of 2% and 4%, and their reuse in five successive fermentations. The immobilized cells with 4% alginate beads were mechanically more stable and able to allow a greater number of reuses, making the process more economical. The fermentation’s consumption of sugars with free cells (control) and immobilized cells showed a similar profile, being completed close to 72 h, while the first use of immobilized cells finished at 96 h. The immobilized cells did not significantly influence some oenological parameters, such as the yield of the consumed sugars/ethanol, the alcohol content, the pH and the total acidity. There was a slight increase in the volatile acidity and a decrease in the production of SO2. The alginate concentrations did not significantly influence either the parameters used to monitor the fermentation process or the characteristics of the mead. Mead fermentations with immobilized cells showed the release of cells into the wort due to the disintegration of the beads, indicating that the matrix used for the yeast’s immobilization should be optimized, considering the mead production medium.

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