Factors affecting plasma progesterone concentration and the retrospective determination of time of ovulation in cyclic mares

2004 ◽  
Vol 61 (2-3) ◽  
pp. 203-214 ◽  
Author(s):  
P. Nagy ◽  
Gy. Huszenicza ◽  
J. Reiczigel ◽  
J. Juhász ◽  
M. Kulcsár ◽  
...  
Keyword(s):  
Progesterone Concentration ◽  
Plasma Progesterone ◽  
Factors Affecting

scholarly journals Saisonale Einflüsse auf die Konzentration von Progesteron im Blutplasma von Müttern der Rasse Schwarzköpfiges Fleischschaf (Kurzmitteilung)

1999 ◽  
Vol 42 (2) ◽  
pp. 183-190
Author(s):  
F. Rehbock ◽  
F. Schneider ◽  
S. Zupp ◽  
S. Grumbach
Keyword(s):  
Blood Plasma ◽  
Corpus Luteum ◽  
Early Pregnancy ◽  
Short Communication ◽  
Progesterone Level ◽  
Ovarian Activity ◽  
Progesterone Concentration ◽  
Plasma Progesterone ◽  
Black Head

Abstract. Title of the paper: Seasonal influences on blood plasma progesterone concentration in ewes of Black head Mutton sheep breed (short communication) In 71 Black head mutton sheeps on pasturing and housing were determined from the year 1995 to 1997 and there in the period from March to October the concentration of blood progesterone for determination of onset of estrus. The level of blood progesterone reported, on literature also, no cycle on this period. The investigation of 6 of this sheeps with ultrasonography had the result of ovarian activity with follicles from 2 mm to 7 mm in diameter, but no cycle was determined. The progesterone level of minimum and maximum on all years differed from March to beginning of September between 0,33 ng/ml and 2,15 ng/ml blood progesterone, after this to October between 0,82 ng/ml and 4,25 ng/ml blood progesterone. The pregnant sheeps detected blood progesterone concentrations from 5,50 ng/ml to 13,55 ng/ml blood progesterone (twins). The corpus luteum activity may be is prevalent on the blood progesterone level up 2 ng/ml and pregnancy up 5 ng/ml blood progesterone. The detectability of blood progesterone and supplemented ultrasonography of ovary possess the possibility recording of onset cycle activity and early pregnancy diagnosis.

Re-used progesterone devices efficiently synchronise oestrus and ovulation after autoclaving process in Toggenburg goats during the breeding season

2015 ◽  
Vol 55 (6) ◽  
pp. 818 ◽  
Author(s):  
J. M. G. Souza-Fabjan ◽  
C. A. A. Torres ◽  
A. L. R. S. Maia ◽  
F. Z. Brandão ◽  
E. Oba ◽  
...  
Keyword(s):  
Breeding Season ◽  
Corpora Lutea ◽  
Device Removal ◽  
Progesterone Concentration ◽  
Plasma Progesterone ◽  
Blood Samples ◽  
Follicle Diameter ◽  
Lactating Females ◽  
Equine Chorionic Gonadotropin

This study compared new and previously used (and autoclaved) progesterone devices for synchronisation of oestrus during the breeding season in Toggenburg goats. Nulliparous (n = 17) or lactating (n = 50) received new intravaginal devices containing 0.3 g progesterone (CONTROL), or similar devices previously used for either 6 (USED6) or 12 (USED12) days and subsequently autoclaved. All goats received 5 mg dinoprost at device insertion and 200 IU equine chorionic gonadotropin 5 days later and all devices were removed after 6 days. After device removal, females were mated by fertile bucks. Ovarian ultrasonography was performed every 12 h after device removal until ovulation detection. Blood samples were collected for determination of plasma progesterone concentration in different moments and intervals (from 7 days before device insertion to 3 days after its removal). There was no difference (P > 0.05) among groups CONTROL, USED6 or USED12 for: oestrus response [75% (18/24), 77% (17/22) or 71% (15/21), respectively]; duration of oestrus (30.7 ± 3.4, 31.8 ± 1.7 or 32.8 ± 3.4 h), percentage of ovulating goats [67% (6/9), 78% (7/9) or 56% (5/9)], ovulation rate (1.3 ± 0.2, 1.4 ± 0.2 or 1.8 ± 0.4 units of corpora lutea), average follicle diameter (6.2 ± 0.1, 6.7 ± 0.1 or 6.8 ± 0.3 mm) and pregnancy rate [54% (13/24), 50% (11/22) or 48% (10/21)]. Plasma progesterone concentrations were not different (P > 0.05) for does among treatments and between nulliparous and lactating females. In conclusion, autoclaved, previously used intravaginal progesterone devices are effective in synchronising oestrus and ovulation in cyclic goats during the breeding season.

PROGESTERONE IN THE HUMAN PERIPHERAL BLOOD IN THE PREOVULATORY PERIOD OF THE MENSTRUAL CYCLE

1967 ◽  
Vol 55 (1) ◽  
pp. 91-96 ◽  
Author(s):  
Benno Runnebaum ◽  
Josef Zander
Keyword(s):  
Menstrual Cycle ◽  
Corpus Luteum ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Progesterone Concentration ◽  
Plasma Progesterone ◽  
Progesterone Secretion ◽  
Graafian Follicle ◽  
The Mean ◽  
Mean Concentration

ABSTRACT Progesterone was determined and identified in human peripheral blood during the preovulatory period of the menstrual cycle, by combined isotope derivative and recrystallization analysis. The mean concentration of progesterone in 1.095 ml of plasma obtained 9 days before ovulation was 0.084 μg/100 ml. However, the mean concentration of progesterone in 1.122 ml of plasma obtained 4 days before ovulation was 0.279 μg/100 ml. These data demonstrate a source of progesterone secretion other than the corpus luteum. The higher plasma-progesterone concentration 4 days before ovulation may indicate progesterone secretion of the ripening Graafian follicle of the ovary.

Modeling of dynamic mosaicism on ring chromosomes in human fibroblasts and IPSCS

2020 ◽  
pp. 12-13
Author(s):  
Т.В. Никитина ◽  
А.А. Кашеварова ◽  
М.М. Гридина ◽  
А.А. Хабарова ◽  
А.Г. Мензоров ◽  
...  
Keyword(s):  
Genetic Structure ◽  
Human Fibroblasts ◽  
Cellular Heterogeneity ◽  
Mitotic Stability ◽  
Factors Affecting ◽  
Ring Chromosomes ◽  
Cell Clones ◽  
Mitotic Instability ◽  
Mitotic Behavior

Митотическая нестабильность кольцевых хромосом может приводить к появлению клеточных клонов с различной генетической структурой. В качестве модели нестабильности кольцевых хромосом в митозе мы использовали фибробласты от пациентов с r(8), r(13), r(18) и r(22) и полученные из них индуцированные плюрипотентные стволовые клетки (ИПСК). Линии ИПСК с r(22) имели относительно стабильный кариотип на протяжении десятков (до 60) пассажей и сохраняли неизменную структуру кольцевой хромосомы. Кариотип линий ИПСК с r(8) и r(18) на ранних пассажах стабильный, планируется его изучение на поздних пассажах. Наибольшее разнообразие кариотипа выявлено в линиях ИПСК с r(13), в которых наблюдали различные перестройки и выраженную клеточную гетерогенность. Определение факторов, влияющих на митотическую стабильность кольцевых хромосом, может иметь значение для консультирования пациентов. Mitotic instability of ring chromosomes can lead to the appearance of cell clones with different genetic structure. IPSCs from fibroblasts of patients with r(8), r(13), r(18), and r(22) were used as a model of ring chromosomes mitotic behavior. Karyotypes of iPSC lines with r(8) and r(18) have so far been evaluated only in the early passages, lines with r(22) have maintained a relatively stable karyotype up to 60 passages. The occurrence of rearrangements and cellular heterogeneity was found characteristic for r(13) iPSCs. The determination of factors affecting the ring chromosomes mitotic stability would be beneficial for the patient’s prognosis.

Examination of Conditions for the Determination of Carbamate Pesticides in Soils

1992 ◽  
Vol 57 (8) ◽  
pp. 1632-1638 ◽  
Author(s):  
Věra Tatarkovičová ◽  
Zdeněk Stránský
Keyword(s):  
Activated Carbon ◽  
Solvent Extraction ◽  
Soil Type ◽  
Extraction Procedure ◽  
Purification Procedure ◽  
Factors Affecting ◽  
Carbamate Pesticides ◽  
Rp Hplc ◽  
Extracting Solvent

The procedure for the determination of carbamate pesticides in soil was optimized. The following factors affecting the final results were investigated: extracting solvent, extraction procedure, extract purification procedure, and soil type. Triple extraction with acetone and purification of the extract on a two-stage purification column containing an activated carbon-silica gel 1+1 mixture were found optimal. The extracts after treatment were analyzed by RP-HPLC with UV detection. The method developed allows carbamate pesticides in soil to be determined at concentrations in excess of 30 μg kg-1.

scholarly journals Expression Profiles of the Progesterone Receptor, Cyclooxygenase-2, Growth Differentiation Factor 9, and Bone Morphogenetic Protein 15 Transcripts in the Canine Oviducts during the Oestrous Cycle

Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 454
Author(s):  
Jaime Palomino ◽  
Javiera Flores ◽  
Georges Ramirez ◽  
Victor H. Parraguez ◽  
Monica De los Reyes
Keyword(s):  
Gene Expression ◽  
Bone Morphogenetic Protein ◽  
Cyclooxygenase 2 ◽  
Oestrous Cycle ◽  
Progesterone Concentration ◽  
Plasma Progesterone ◽  
Morphogenetic Protein ◽  
Growth Differentiation Factor 9 ◽  
Bone Morphogenetic Protein 15 ◽  
Cox 2

The gene expression in the canine oviduct, where oocyte maturation, fertilization, and early embryonic development occur, is still elusive. This study determined the oviductal expression of (PR), cyclooxygenase-2 (COX-2), growth differentiation factor 9 (GDF-9), and bone morphogenetic protein 15 (BMP-15) during the canine oestrous cycle. Samples were collected from bitches at anoestrus (9), proestrus (7), oestrus (8), and dioestrus (11), after routine ovariohysterectomy and the ovarian surface structures and plasma progesterone concentration evaluated the physiological status of each donor. The oviductal cells were isolated and pooled. Total RNA was isolated, and gene expression was assessed by qPCR followed by analysis using the t-test and ANOVA. The PR mRNA increased (P < 0.05) from the anoestrus to dioestrus with the plasma progesterone concentration (r = 0.8). COX-2 mRNA expression was low in the anoestrus and proestrus, and negligible in the oestrus, while it was around 10-fold higher (P < 0.05) in the dioestrus. The GDF-9 mRNA was expressed during all phases of the oestrous cycle and was most abundant (P < 0.05) during oestrus phase. The BMP-15 mRNA decreased (P < 0.05) in the anoestrus and proestrus phases. Thus, the transcripts were differentially expressed in a stage-dependent manner, suggesting the importance of oestrous cycle regulation for successful reproduction in dogs.

Fish Price Determination Around Lake Victoria, Tanzania: Analysis of Factors Affecting Fish Landing Price

2021 ◽  
Vol 22 (2) ◽  
pp. 348-363
Author(s):  
Damian Boniface Sambuo ◽  
Stephen Kirama ◽  
Kitala Malamsha
Keyword(s):  
Lake Victoria ◽  
Regulatory Body ◽  
Price Determination ◽  
Cross Sectional ◽  
Factors Affecting ◽  
Fish Market ◽  
Fishery Policy ◽  
The Government ◽  
And Performance

Determination of fish landing price is important, as the same contributes to the structure, conduct and performance of the fish market in Lake Victoria. Determination of relevant landing price is a gap to console between fishermen, agents (middlemen), processors and the government. The main objective of this study was therefore to examine fish price determination. Specifically, to examine the methods for fish price determination and analyse factors that affect fish landing price in Lake Victoria, a cross-sectional design was employed, and 300 respondents were randomly selected from two district councils, namely, Sengerema and Buchosa. Both qualitative and quantitative data were analysed using descriptive statistics and inferential analysis. Findings show that landing price is determined through formal negotiation with processors, consultation with other traders, informal negotiation with buyers and Beach Management Unit (BMU). The study concluded that these are the common methods used to determine landing prices. Also, distance from fishing to onshore landing centres, market information channels, age and experiences of the fishermen are the factors significantly found affecting landing price. It is recommended that the mechanism for setting up fishery price, fish market structure, fishery information and the formation of fishery regulatory body needs fishery policy and sector reforms that mark the determination of fish landing price.

scholarly journals On factors affecting surface free energy of carbon black for reinforcing rubber

2020 ◽  
Vol 9 (1) ◽  
pp. 170-181 ◽  
Author(s):  
Shangyong Zhang ◽  
Ruipeng Zhong ◽  
Ruoyu Hong ◽  
David Hui
Keyword(s):  
Free Energy ◽  
Carbon Black ◽  
Surface Free Energy ◽  
Surface Activity ◽  
Gas Flow ◽  
Influential Factors ◽  
Chromatographic Column ◽  
Factors Affecting ◽  
Carrier Gas Flow

AbstractThe surface activity of carbon black (CB) is an important factor affecting the reinforcement of rubber. The quantitative determination of the surface activity (surface free energy) of CB is of great significance. A simplified formula is obtained to determine the free energy of CB surface through theoretical analysis and mathematical derivation. The surface free energy for four kinds of industrial CBs were measured by inverse gas chromatography, and the influential factors were studied. The results showed that the aging time of the chromatographic column plays an important role in accurate measurement of the surface free energy of CB, in comparison with the influences from the inlet pressure and carrier gas flow rate of the chromatographic column filled with CB. Several kinds of industrial CB were treated at high temperature, and the surface free energy of CB had a significant increase. With the increase of surface free energy, the maximum torque was decreased significantly, the elongation at break tended to increase, the heat generation of vulcanizates was increased, and the wear resistance was decreased.

Factors affecting in vitro and in vivo antioxidant effects. Experimental conditions and nature of oxidants determine antioxidant efficacy

2021 ◽  
pp. 1-9
Author(s):  
Etsuo Niki
Keyword(s):  
Biological Molecules ◽  
Experimental Conditions ◽  
Factors Affecting ◽  
Beneficial Effects ◽  
Multiple Oxidants ◽  
Antioxidant Effects

Reactive oxygen and nitrogen species have been implicated in the onset and progression of various diseases and the role of antioxidants in the maintenance of health and prevention of diseases has received much attention. The action and effect of antioxidants have been studied extensively under different reaction conditions in multiple media. The antioxidant effects are determined by many factors. This review aims to discuss several important issues that should be considered for determination of experimental conditions and interpretation of experimental results in order to understand the beneficial effects and limit of antioxidants against detrimental oxidation of biological molecules. Emphasis was laid on cell culture experiments and effects of diversity of multiple oxidants on antioxidant efficacy.

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