O.073 Failures and suboptimal positions of gravitational valves at different implantation sites (retroauricular vs. thoracal)

2008 ◽  
Vol 110 ◽  
pp. S20
Author(s):  
B. Vienenkötter ◽  
A. Unterberg ◽  
A. Aschoff
Keyword(s):  
Implantation Sites

Effect of Exposure to Mixture of Heavy Metals Arsenic, Cadmium and Lead on Reproductive Function and Oxidative Stress in Female Rats

2017 ◽  
Vol 9 (03) ◽  
Author(s):  
Choudhuri D. ◽  
Bhattacharjee T.
Keyword(s):  
Oxidative Stress ◽  
Heavy Metals ◽  
Ascorbic Acid ◽  
Reproductive Function ◽  
Reproductive Organs ◽  
Female Rats ◽  
Corpora Lutea ◽  
Oestrus Cycle ◽  
Implantation Sites ◽  
Live Fetus

Background : Toxicological consequences arising from exposure to mixtures of heavy metals especially at low, chronic and environmentally relevant doses are poorly recognised. In the present study, we evaluated effects of chronic exposure to combinations of three metals arsenic (As), cadmium (Cd) and lead (Pb) present frequently in drinking water on reproductive function and oxidative damage caused to reproductive organs of female rats. Method : Female rats were exposed to mixture of metals (As, Cdand Pb) for 90 consecutive days. The gain in body weight and weight of reproductive organs were recorded following autopsy on 91 stday. The oestrus cycle were monitored during entire treatment period. Numbers of corpora lutea, implantation sites, live fetus and survival of the fetus were evaluated in rats mated successfully with untreated male after completion of their respective treatment. Ovarian cholesterol, protein, ascorbic acid and enzyme Δ 5 -3β HSD levels were estimated. Serum levels of steroid hormones oestrogen and progesterone were estimated. Histopathological picture of both ovary and uterus were assessed. Levels of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidise (GPX) activity, amount of reduced glutathione (GSH) and malondyaldehyde (MDA) in blood, ovary and uterus were measured as biomarkers of oxidative stress. Results : The treated rats showed reduced body weight gain and reduction in the weight of ovary and uterus. Oestrus cycle was disrupted with continuous diestrous in treated animals. Number of corpora lutea, implantation sites and live fetus and the survival of fetus evaluate were reduced significantly in treated groups. The levels of ovarian cholesterol and ascorbic acid increased in treated rats with decrease Δ5 -3β HSD level. There was reduction in serum level of both the ovarian steroid hormones oestrogen and progesterone. The protein levels did not differ between the groups. There was a significant increase in levels of MDA and decrease in levels of all the antioxidant enzymes in treated group. Conclusion : The results revealed there was disruption to reproductive functions with decrease in stereoidogenic activity and associated oxidative stress in female rats treated with combination of mixture of metals (Cd, As and Pb) at low dose for 90 consecutive days.

scholarly journals Cell–Cell Communication at the Embryo Implantation Site of Mouse Uterus Revealed by Single-Cell Analysis

2021 ◽  
Vol 22 (10) ◽  
pp. 5177
Author(s):  
Yi Yang ◽  
Jia-Peng He ◽  
Ji-Long Liu
Keyword(s):  
Single Cell ◽  
Molecular Mechanism ◽  
Single Cell Analysis ◽  
Embryo Implantation ◽  
Cell Communication ◽  
Human Reproduction ◽  
Mouse Uterus ◽  
Implantation Sites ◽  
Low Efficiency ◽  
Signaling Interactions

As a crucial step for human reproduction, embryo implantation is a low-efficiency process. Despite rapid advances in recent years, the molecular mechanism underlying embryo implantation remains poorly understood. Here, we used the mouse as an animal model and generated a single-cell transcriptomic atlas of embryo implantation sites. By analyzing inter-implantation sites of the uterus as control, we were able to identify global gene expression changes associated with embryo implantation in each cell type. Additionally, we predicted signaling interactions between uterine luminal epithelial cells and mural trophectoderm of blastocysts, which represent the key mechanism of embryo implantation. We also predicted signaling interactions between uterine epithelial-stromal crosstalk at implantation sites, which are crucial for post-implantation development. Our data provide a valuable resource for deciphering the molecular mechanism underlying embryo implantation.

Effects of the aqueous extract of Dicliptera verticillata on fertility and different stages of gestation in female rats

Zygote ◽  
2021 ◽  
pp. 1-7
Author(s):  
Stéphanie Ouabo Meguem ◽  
Landry Lienou Lienou ◽  
Marie Stéphanie Chekem Goka ◽  
Richard Simo Tagne ◽  
Didiane Mefokou Yemele ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Aqueous Extract ◽  
Implantation Rate ◽  
The Other ◽  
Female Rats ◽  
High Dose ◽  
Immature Female ◽  
Implantation Sites ◽  
Parameters Analysis ◽  
Hepatic Proteins

Summary Dicliptera verticillata is a medicinal plant traditionally used in western Cameroon to cure female infertility. This experiment was designed to assess the effects of the aqueous extract of Dicliptera verticillata (AEDv) on fertility and gestation in female rats. Oral increasing doses of AEDv were administered to immature female rats over 20 d. After this time, some animals were mated with fertile males and some fertility parameters were assayed; the other animals were euthanized for preliminary toxicity parameters analysis. The effects of AEDv on the different stages of gestation were assayed on selected animals previously controlled for estrous cycle regularity and mated. AEDv led to an increase in serum, uterine and ovarian proteins as well as in ovarian and uterine weights (P < 0.05) in immature female rats. Hepatic proteins significantly decreased (P < 0.01) in high dose-treated animals (50 and 100 mg/kg) compared with controls. The number of implantation sites and the fertility rate were significantly lower (P < 0.05), while the antifertility activity increased significantly (P < 0.05) in treated rats compared with controls. When administered from the 1st to the 5th day of pregnancy, AEDv led to a decrease of more than 60% in the implantation rate in high dose-treated rats (50, 100, and 400 mg/kg). From the 6th to the 9th day, the implantation, gestation rates and the number of fetuses decreased significantly in all treated groups. From the 11th to the 20th day, a 50% resorption and decrease in gestation rate were reported in 50 mg/kg dose-treated animals. AEDv possesses weak contraceptive and abortifacient effects during pregnancy.

scholarly journals Implantation sites after embryo transfer into the central area of the uterine cavity

2006 ◽  
Vol 13 (4) ◽  
pp. 541-546 ◽  
Author(s):  
M Cavagna ◽  
P Contart ◽  
CG Petersen ◽  
AL Mauri ◽  
AMC Martins ◽  
...  
Keyword(s):  
Embryo Transfer ◽  
Central Area ◽  
Uterine Cavity ◽  
Implantation Sites

Platelet-activating factor-antagonists reduce implantation in mice at low doses only

1995 ◽  
Vol 7 (1) ◽  
pp. 51 ◽  
Author(s):  
C O'Neill
Keyword(s):  
Early Pregnancy ◽  
Embryo Implantation ◽  
Platelet Activating Factor ◽  
Implantation Rate ◽  
Corpora Lutea ◽  
Detailed Knowledge ◽  
Low Doses ◽  
Paf Antagonists ◽  
Implantation Sites ◽  
Web 2086

The effects of a number of platelet-activating factor (PAF)-antagonists on embryo implantation were investigated. Mice were treated from Day 1 to Day 4 of pregnancy with three defined PAF-antagonists: SRI 63 441, BN 52021, and WEB 2086. Necroscopies were performed on Day 8 and the number of implantation sites, the implantation rate (number of implanted embryos compared with the number of corpora lutea) and the proportion of animals pregnant were determined. Each agent caused a reduction in the number of implantation sites at relatively low doses. The dose that had a maximum contragestational effect was 40 micrograms, 10 micrograms and 10 micrograms (per 30 g bodyweight per day) for SRI 63 441, WEB 2086 and BN 52021 respectively. This contragestational effect was completely lost at twice (SRI 63 441), five times (WEB 2086) and ten times (BN 52021) the most effective dose. Treatment with WEB 2086 on the day of implantation (Day 4) by intraperitoneal injection or instillation into the uterus only did not significantly reduce the implantation rate and neither did treatment after implantation (Days 5-8). The results show that the pharmacology of PAF-antagonists in early pregnancy is not simple. An understanding of the actions of these agents in early pregnancy will require a detailed knowledge of their pharmacokinetics, pharmacodynamics and targets of action in early pregnancy.

scholarly journals The uterine expression of SEC63 gene is up-regulated at implantation sites in association with the decidualization during the early pregnancy in mice

2009 ◽  
Vol 7 (1) ◽  
pp. 12 ◽  
Author(s):  
Ren-wei Su ◽  
Zhao-gui Sun ◽  
Yue-chao Zhao ◽  
Qiu-ju Chen ◽  
Zeng-ming Yang ◽  
...  
Keyword(s):  
Early Pregnancy ◽  
Implantation Sites

scholarly journals Comparative Transcriptomic Analysis of Embryo Implantation in Mice and Rats

2018 ◽  
Vol 50 (2) ◽  
pp. 668-678 ◽  
Author(s):  
Wen-Qian Zhang ◽  
Miao Zhao ◽  
Ming-Yu Huang ◽  
Ji-Long Liu
Keyword(s):  
Differentially Expressed Genes ◽  
Molecular Mechanisms ◽  
Embryo Implantation ◽  
Differentially Expressed ◽  
Rat Uterus ◽  
Mouse Uterus ◽  
Pathway Network ◽  
Implantation Sites ◽  
High Selection ◽  
Transcriptomic Changes

Background/Aims: Embryo implantation is an essential process for eutherian pregnancy, but this process varies across eutherians. The genomic mechanisms that led to the emergence and diversification of embryo implantation are largely unknown. Methods: In this study, we analyzed transcriptomic changes during embryo implantation in mice and rats by using RNA-seq. Bioinformatics and evolutionary analyses were performed to characterize implantation-associated genes in these two species. Results: We identified a total of 518 differentially expressed genes in mouse uterus during implantation, of which 253 genes were up-regulated and 265 genes were down-regulated at the implantation sites compared with the inter-implantation sites. In rat uterus, there were 374 differentially expressed genes, of which 284 genes were up-regulated and 90 genes were down-regulated. A cross-species comparison revealed that 92 up-regulated genes and 20 down-regulated genes were shared. The differences and similarities between mice and rats were investigated further at the gene ontology, pathway, network, and causal transcription factor levels. Additionally, we found that embryo implantation might have evolved through the recruitment of ancient genes into uterine expression. The evolutionary rates of the differentially expressed genes in mouse and rat uterus were significantly lower than those of the non-changed genes, indicating that implantation-related genes are evolutionary conserved due to high selection pressure. Conclusion: Our study provides insights into the molecular mechanisms involved in the evolution of embryo implantation.

Altered expression patterns of circular RNAs between implantation sites and interimplantation sites in early pregnant mice

2018 ◽  
Vol 234 (6) ◽  
pp. 9862-9872 ◽  
Author(s):  
Shuang Zhang ◽  
Yubin Ding ◽  
Junlin He ◽  
Juanjuan Zhang ◽  
Xueqing Liu ◽  
...  
Keyword(s):  
Expression Patterns ◽  
Circular Rnas ◽  
Altered Expression ◽  
Pregnant Mice ◽  
Implantation Sites

CHANGES IN ENZYMES OF CARBOHYDRATE METABOLISM IN RAT UTERUS DURING EARLY PREGNANCY

1971 ◽  
Vol 68 (4) ◽  
pp. 805-816 ◽  
Author(s):  
M. A. H. Surani ◽  
P. J. Heald
Keyword(s):  
Energy Requirement ◽  
Lipid Synthesis ◽  
Dry Weight ◽  
Glycolytic Enzymes ◽  
Rat Uterus ◽  
Malic Dehydrogenase ◽  
Phosphogluconate Dehydrogenase ◽  
Tissue Changes ◽  
Implantation Sites ◽  
Glucose 6 Phosphate Dehydrogenase

ABSTRACT The enzymes phosphofructokinase (PFK), pyruvate kinase (PK), isocitric dehydrogenase (ICDH), malic dehydrogenase (MDH), glucose-6-phosphate dehydrogenase (G-6PDH) and 6-phosphogluconate dehydrogenase (6PGDH) have been measured in rat uterus during the first 9 days of pregnancy. It was found that after implantation on day 6, the activities of PFK and PK (the key glycolytic enzymes) increased in terms of dry weight — and in terms of protein in the implantation sites, but decreased in non-implanted tissue. The pentose shunt enzymes changed similarly to those of the glycolytic enzymes. ICDH activity increased in the non-implanted tissue and decreased in the implanted tissue. Changes in malic dehydrogenase were extremely variable and did not show a consistent pattern. Administration of Actinomycin D on day 6 of pregnancy abolished the increase in PK and PFK in the implantation sites and indeed led to a major decrease in activity. This implies that the increased PK and PFK in the implantation sites, arise from a DNA dependent RNA directed synthesis of new enzyme protein. The results are discussed in relation to the energy requirement of the decidualising tissue and the need for increased pentose for RNA synthesis. It is suggested that the extra NADPH resulting from the pentose shunt is involved in increased lipid synthesis.

scholarly journals Evaluation of abortifacient efficacy of Rumex steudelli (Tult) root traditionally used medicinal plant in South West Ethiopia

2015 ◽  
Vol 4 (4) ◽  
pp. 221-223
Author(s):  
Alemu Tadesse Feroche ◽  
Keyword(s):  
Body Weight ◽  
Alcoholic Extract ◽  
Phytochemical Screening ◽  
Albino Rat ◽  
Pregnant Rats ◽  
Plant Medicine ◽  
South West ◽  
Implantation Sites ◽  
South West Ethiopia ◽  
Western Ethiopia

The practice of traditional medicine for the control of fertility in most part of South Western Ethiopia is based on the uses of plant medicine for many years. The present study was carried out in female albino rat to explore the abortifacient activity of the Rumex steudelli roots. Pregnant rats weighing 140- 200 g were randomized into 7 groups. Rats were laprotomised on 10th day of pregnancy and the two horns of uteri were examined to determine the implantation sites. The administration of 400 mg/kg body weight of the aqueous extract of Rumex steudelli resulted in 95% abortion. The 200 and 400 mg/kg body weight alcoholic extract showed 100% fetus abortion. The phytochemical screening of the roots of Rumex steudelli revealed the presence of flavonoids, phenolics, alkaloids, steroids, tannins and saponins.

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