Prostaglandin E (PGE) and interleukin-1β (IL-1β) levels in gingival crevicular fluid during human orthodontic tooth movement

Abstract Objective: To determine whether interleukin 1β (IL-1β) levels are elevated around microscrew implants that are used as anchorage for tooth movement. Materials and Methods: Ten young adults, aged 16.3 ± 2.5 years and with all four premolars extracted , comprised the study group. Twenty maxillary microscrew implants were placed bilaterally in the alveolar bone between the maxillary second premolars and first molars as anchorage units for distal movement of the maxillary canines. The maxillary canines served as the treatment group, and the microscrew implants were designated as the implant group. The mandibular canines were used as controls. Peri-microscrew implant crevicular fluid (MICF) and gingival crevicular fluid (GCF) were collected at the beginning of tooth movement (2 weeks after implant placement); at 24, 48, and 168 hours later; and on days 14 and 21. An automated enzyme immunoassay was used to measure 1L-1β in the MICF and the GCF. Results: The mean 1L-1β level in the treatment group was significantly elevated at 24 hours (P = .003 < .05) and 48 hours (P = .003 < .05), whereas the levels in the control and implant groups did not change significantly during the experimental period. Also, the mean 1L-1β level of the treatment group was significantly higher that in both the control and implant groups at 24 and 48 hours. Conclusions: The microscrew implants did not demonstrate increased 1L-1β levels during tooth movement. This supports the concept that microscrew implants might be useful as absolute anchorage devices.

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Effect of ovarian activity on orthodontic tooth movement and gingival crevicular fluid levels of interleukin-1β and prostaglandin E2 in cats

The Angle Orthodontist ◽

10.2319/012912-78.1 ◽

2012 ◽

Vol 83 (1) ◽

pp. 70-75 ◽

Cited By ~ 12

Author(s):

Ahmet Arif Celebi ◽

Serhat Demirer ◽

Bulent Catalbas ◽

Sevket Arikan

Keyword(s):

Prostaglandin E2 ◽

Tooth Movement ◽

Gingival Crevicular Fluid ◽

Orthodontic Tooth Movement ◽

Interleukin 1Β ◽

Enzyme Linked Immunosorbent Assay ◽

Ovarian Activity ◽

Coil Spring ◽

Significant Difference ◽

Crevicular Fluid

Abstract Objective: To evaluate whether there is any correlation between ovarian activity and two potent bone-resorbing mediators (prostaglandin E2 [PGE2], interleukin-1β [IL-1β]) secreted from the gingival crevicular fluid (GCF) during orthodontic tooth movement. Materials and Methods: Eighteen female cats were included in this study. Animals were randomly divided into three groups (estrous, anestrous, and ovariectomized groups), each having six queens. Estrous was induced by administration of 150 IU equine chorionic gonadotropin (eCG) to queens of the estrous group. A closed-coil spring, applied with 80 g of tipping force to the canine, was attached between the maxillary canine and mini-implant. GCF was collected on days 0, 6, and 12 from each cat to examine PGE2 and IL-1β during orthodontic tooth movement in cats. The PGE2 and IL-1β levels were determined with enzyme-linked immunosorbent assay. Results: There was no significant difference (P > .05) between anestrous and the ovariectomized groups in terms of tooth movement on days 6 and 12 of distalization. In contrast, tooth movement in the estrous group was lower (P < .05) than in the remaining two groups (anestrous and ovariectomized). The mean PGE2 and IL-1β levels of the canine teeth of the estrous groups were significantly lower than the remaining two groups on days 6 and 12 (P < .05) of coil spring applications. Conclusions: These results indicate that ovarian activity can affect orthodontic tooth movement and GCF levels of IL-1β and PGE2 in cats.

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Low-level laser therapy increases interleukin-1β in gingival crevicular fluid and enhances the rate of orthodontic tooth movement

American Journal of Orthodontics and Dentofacial Orthopedics ◽

10.1016/j.ajodo.2018.01.012 ◽

2018 ◽

Vol 154 (4) ◽

pp. 535-544.e5 ◽

Cited By ~ 15

Author(s):

Alissa Maria Varella ◽

Ameet V. Revankar ◽

Anand K. Patil

Keyword(s):

Laser Therapy ◽

Tooth Movement ◽

Gingival Crevicular Fluid ◽

Orthodontic Tooth Movement ◽

Interleukin 1Β ◽

Low Level Laser Therapy ◽

Low Level ◽

Low Level Laser ◽

Level Laser ◽

Crevicular Fluid

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Evaluation of IL1β and IL6 Gingival Crevicular Fluid Levels during the Early Phase of Orthodontic Tooth Movement in Adolescents and Young Adults

Applied Sciences ◽

10.3390/app11020521 ◽

2021 ◽

Vol 11 (2) ◽

pp. 521

Author(s):

Simina Chelărescu ◽

Petra Șurlin ◽

Mioara Decusară ◽

Mădălina Oprică ◽

Eugen Bud ◽

...

Keyword(s):

Young Adults ◽

Orthodontic Treatment ◽

Tooth Movement ◽

Gingival Crevicular Fluid ◽

Early Stage ◽

Statistical Tests ◽

Orthodontic Tooth Movement ◽

Adolescents And Young Adults ◽

Periodontal Tissues ◽

Crevicular Fluid

Background: The crevicular fluid analysis represents a useful diagnosis tool, with the help of which noninvasive cellular metabolic activity can be analyzed. The aim of the study is to investigate comparatively IL1β and IL6 in the gingival crevicular fluid of clinically healthy adolescents and young adults during the acute phase of orthodontic treatment. Methods: Gingival crevicular fluid was collected from 20 patients (aged between 11 and 28) undergoing orthodontic treatment. Measurements were taken before (T0) and after 24 h after distalization forces were activated (T1). IL1β and IL 6 were analyzed using Elisa tests. The statistical tests used were two-sided t tests. Results: Between the two time periods there was a significant raise both in the crevicular fluid rate (0.57 µL at T0 vs. 0.95 µL at T1, p = 0.001) and in IL1β levels (15.67 pg/µL at T0 vs. 27.94 pg/µL at T1, p = 0.009). We were able to identify IL6 only in a third of the sites. There is a significantly increased level of ILβ at T1 in adolescents, more than in young adults (42.96 pg/µL vs. 17.93 pg/µL, p = 0.006). Conclusions: In the early stage of orthodontic treatment, the periodontal tissues of adolescents are more responsive to orthodontic forces than those of young adults.

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Application of Vibrational Spectroscopies in the Qualitative Analysis of Gingival Crevicular Fluid and Periodontal Ligament during Orthodontic Tooth Movement

Journal of Clinical Medicine ◽

10.3390/jcm10071405 ◽

2021 ◽

Vol 10 (7) ◽

pp. 1405

Author(s):

Fabrizia d’Apuzzo ◽

Ludovica Nucci ◽

Ines Delfino ◽

Marianna Portaccio ◽

Giuseppe Minervini ◽

...

Keyword(s):

Periodontal Ligament ◽

Tooth Movement ◽

Gingival Crevicular Fluid ◽

Orthodontic Tooth Movement ◽

High Sensitivity ◽

Data Analysis Procedure ◽

Vibrational Spectroscopies ◽

Composition Structure ◽

The Individual ◽

Crevicular Fluid

Optical vibrational techniques show a high potentiality in many biomedical fields for their characteristics of high sensitivity in revealing detailed information on composition, structure, and molecular interaction with reduced analysis time. In the last years, we have used these techniques for investigating gingival crevicular fluid (GCF) and periodontal ligament (PDL) during orthodontic tooth treatment. The analysis with Raman and infrared signals of GCF and PDL samples highlighted that different days of orthodontic force application causes modifications in the molecular secondary structure at specific wavenumbers related to the Amide I, Amide III, CH deformation, and CH3/CH2. In the present review, we report the most relevant results and a brief description of the experimental techniques and data analysis procedure in order to evidence that the vibrational spectroscopies could be a potential useful tool for an immediate monitoring of the individual patient’s response to the orthodontic tooth movement, aiming to more personalized treatment reducing any side effects.

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