Molecular Comparison of Mycoplasma hominis Strains Isolated from Colonized Women and Women with Various Urogenital Infections

2000 ◽  
Vol 289 (8) ◽  
pp. 879-888 ◽  
Author(s):  
U. Busch ◽  
H. Nitschko ◽  
F. Pfaff ◽  
B. Henrich ◽  
J. Heesemann ◽  
...  
Keyword(s):  
Mycoplasma Hominis ◽  
Urogenital Infections ◽  
Molecular Comparison

scholarly journals Prevalence and Antimicrobial Susceptibility of Mycoplasma hominis and Ureaplasma Species in Nonpregnant Female Patients in South Korea Indicate an Increasing Trend of Pristinamycin-Resistant Isolates

2020 ◽  
Vol 64 (10) ◽  
Author(s):  
Ji Yong Lee ◽  
Jeong Seon Yang
Keyword(s):  
Risk Factors ◽  
Confidence Interval ◽  
South Korea ◽  
Antimicrobial Susceptibility ◽  
Odds Ratio ◽  
Treatment Strategies ◽  
Mycoplasma Hominis ◽  
Content Type ◽  
Female Patients ◽  
Urogenital Infections

ABSTRACT Mycoplasma hominis and Ureaplasma species, commonly found in the lower urogenital tract, have been associated with various urogenital infections. This study aimed to estimate the prevalence and antimicrobial susceptibility trend of M. hominis and Ureaplasma sp. in female patients and to evaluate the risk factors for the acquisition of pristinamycin-resistant mycoplasma. Endocervical swab specimens obtained between March 2016 and December 2018 were analyzed using a Mycoplasma IST2 kit. Because pristinamycin and josamycin are not available in South Korea, we conducted an age- and date-matched case-control study to evaluate the risk factors for the acquisition of pristinamycin-resistant isolates. Among 4,035 specimens, 1,589 (39.4%) cases were positive for genital mycoplasma, which included 49 (3.1%) cases of M. hominis, 1,243 (78.2%) cases of Ureaplasma sp., and 297 (18.7%) cases of both M. hominis and Ureaplasma species. Based on antimicrobial susceptibility tests, the antibiotic susceptible rate of both M. hominis and Ureaplasma species to pristinamycin decreased annually during the study period (100%, 97.1%, and 87.3% for 2016, 2017, and 2018, respectively, P < 0.001). According to a multivariate analysis, josamycin resistance (odds ratio, 7.18; 95% confidence interval, 1.20 to 43.00; P = 0.027) and coinfection (odds ratio, 145.38; 95% confidence interval, 21.80 to 3,017.23; P < 0.001) with Candida species were independent risk factors for the acquisition of pristinamycin-resistant isolates. Antibiotic-resistant genital mycoplasmas have been gradually increasing annually. Nationwide surveillance, proper antibiotic stewardship, and appropriate culture-based treatment strategies are required to control this upcoming threat.

scholarly journals Results from a large cross-sectional study assessing Chlamydia trachomatis, Ureaplasma spp. and Mycoplasma hominis urogenital infections in patients with primary infertility

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Daniela Andrea Paira ◽  
Guillermo Molina ◽  
Andrea Daniela Tissera ◽  
Carolina Olivera ◽  
Rosa Isabel Molina ◽  
...  
Keyword(s):  
Chlamydia Trachomatis ◽  
Clinical Importance ◽  
Mycoplasma Hominis ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Cross Sectional ◽  
Urogenital Infection ◽  
Primary Infertility ◽  
Urogenital Infections ◽  
Male Patients

AbstractFemale and male infertility have been associated to Chlamydia trachomatis, Ureaplasma spp. and Mycoplasma hominis urogenital infections. However, evidence from large studies assessing their prevalence and putative associations in patients with infertility is still scarce. The study design was a cross-sectional study including 5464 patients with a recent diagnosis of couple’s primary infertility and 404 healthy control individuals from Cordoba, Argentina. Overall, the prevalence of C. trachomatis, Ureaplasma spp. and M. hominis urogenital infection was significantly higher in patients than in control individuals (5.3%, 22.8% and 7.4% vs. 2.0%, 17.8% and 1.7%, respectively). C. trachomatis and M. hominis infections were significantly more prevalent in male patients whereas Ureaplasma spp. and M. hominis infections were more prevalent in female patients. Of clinical importance, C. trachomatis and Ureaplasma spp. infections were significantly higher in patients younger than 25 years. Moreover, Ureaplasma spp. and M. hominis infections were associated to each other in either female or male patients being reciprocal risk factors of their co-infection. Our data revealed that C. trachomatis, Ureaplasma spp. and M. hominis are prevalent uropathogens in patients with couple’s primary infertility. These results highlight the importance of including the screening of urogenital infections in the diagnostic workup of infertility.

scholarly journals Occurrence of Ureaplasma urealyticumin in Women in the Northeast of Iran: Characterization of Resistance Trends

2018 ◽  
Vol 9 (4) ◽  
pp. 274-279
Author(s):  
Jalal Mardaneh ◽  
Alireza Mohammadzadeh ◽  
Mahdieh Sadat Alavi ◽  
Mahdieh Zendehdel ◽  
Narjes Bahri ◽  
...  
Keyword(s):  
Susceptibility Testing ◽  
Ureaplasma Urealyticum ◽  
Mycoplasma Hominis ◽  
Vaginal Swab ◽  
Drug Resistant ◽  
Urogenital Infections ◽  
Positive Rate ◽  
Single Positive ◽  
Resistance Rates

Objectives: The present study surveyed the prevalence of antibiotic resistance among Ureaplasma urealyticum in isolates from Gonabad (in the northeast of Iran) including susceptibility testing for U. urealyticum to different antibiotics. Materials and Methods: In this research, a total of 95 vaginal swab specimens were aseptically collected from women who were admitted to the Bohlool Teaching Hospital and Jahad Daneshgahi Center from April 2016 to April 2017. Culture and subsequently antibiotic susceptibility testing were performed according to the Mycoplasma IST 2 kit. Then the cupules were read and interpreted in 24 and 48 hours according to kit guidelines. Results: In the studied patients, 38 (40.4 %), 12 (12.8 %), and 11 (11.7%) cases were single positive for U. urealyticum, single positive for Mycoplasma hominis (M. hominis), and dually positive for U. urealyticum and M. hominis, respectively. The positive rates of genital U. urealyticum in the symptomatic and asymptomatic groups were 86.8% and 13.2%, respectively. The highest positive rate (42.1%) was found in the 26-30-year-old group. In addition, tetracycline (TET) and doxycycline (DOT) were the most effective antibiotics against isolates, and one strain was multi-drug resistant. The U. urealyticum resistance rates were more than 39% to erythromycin and pristinamycin, and more than 55% to ciprofloxacin. All U. urealyticum isolates with <104 CFU/specimen were sensitive to all tested drugs. Conclusions: Although the emerging resistance to TETs among our isolates is alarming, these data show that the standard therapeutic regimen for urogenital infections caused by U. urealyticum is DOT, TET, and clarithromycin, leading to better outcomes in most respective patients.

scholarly journals Unexpected Mycoplasma hominis infection in two renal transplant recipients traced back to the same donor by whole-genome sequencing

2020 ◽  
Author(s):  
V. Hinić ◽  
H. M. B. Seth-Smith ◽  
S. Damm ◽  
P. Amico ◽  
N. Khanna ◽  
...  
Keyword(s):  
Renal Transplant ◽  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Mycoplasma Hominis ◽  
Organ Donor ◽  
Renal Transplant Recipients ◽  
Whole Genome ◽  
Transplant Recipients ◽  
Urogenital Infections ◽  
Invasive Infections

AbstractMycoplasma hominis is a common colonizer of the lower genitourinary tract. Although its clinical relevance for causing urogenital infections in immunocompetent individuals is controversial, this bacterium has been involved in severe invasive infections in allograft recipients. In this report, we describe two cases of M. hominis infection in two young renal transplant recipients within the first month post-transplant. Although at first no epidemiological link between the two cases had been suspected, whole-genome sequencing (WGS) analysis showed that both isolates were identical, highly suggestive of an origin with the common organ donor.

scholarly journals Determination of In Vitro Antimicrobial Susceptibility for Lefamulin (Pleuromutilin) for Ureaplasma Spp. and Mycoplasma hominis

Antibiotics ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 1370
Author(s):  
Oliver Spiller-Boulter ◽  
Susanne Paukner ◽  
Ian Boostrom ◽  
Kirsty Sands ◽  
Edward A. R. Portal ◽  
...  
Keyword(s):  
Ureaplasma Urealyticum ◽  
Acquired Resistance ◽  
Mycoplasma Hominis ◽  
Fold Increase ◽  
23S Rrna ◽  
Ureaplasma Parvum ◽  
Urogenital Infections ◽  
Ureaplasma Spp

Lefamulin is the first of the pleuromutilin class of antimicrobials to be available for therapeutic use in humans. Minimum inhibitory concentrations of lefamulin were determined by microbroth dilution for 90 characterised clinical isolates (25 Ureaplasma parvum, 25 Ureaplasma urealyticum, and 40 Mycoplasma hominis). All Mycoplasma hominis isolates possessed lefamulin MICs of ≤0.25 mg/L after 48 h (MIC50/90 of 0.06/0.12 mg/L), despite an inherent resistance to macrolides; while Ureaplasma isolates had MICs of ≤2 mg/L after 24 h (MIC50/90 of 0.25/1 mg/L), despite inherent resistance to clindamycin. Two U. urealyticum isolates with additional A2058G mutations of 23S rRNA, and one U. parvum isolate with a R66Q67 deletion (all of which had a combined resistance to macrolides and clindamycin) only showed a 2-fold increase in lefamulin MIC (1–2 mg/L) relative to macrolide-susceptible strains. Lefamulin could be an effective alternative antimicrobial for treating Ureaplasma spp. and Mycoplasma hominis infections irrespective of intrinsic or acquired resistance to macrolides, lincosamides, and ketolides. Based on this potent in vitro activity and the known good, rapid, and homogenous tissue penetration of female and male urogenital tissues and glands, further exploration of clinical efficacy of lefamulin for the treatment of Mycoplasma and Ureaplasma urogenital infections is warranted.

scholarly journals Chlamydia trachomatis, Ureaplasma urealyticum and Mycoplasma hominis urogenital infections in patients with infertility. Results from a large cross-sectional study.

2021 ◽  
Author(s):  
Daniela Andrea Paira ◽  
Guillermo Molina ◽  
Andrea Daniela Tissera ◽  
Carolina Olivera ◽  
Rosa Isabel Molina ◽  
...  
Keyword(s):  
Chlamydia Trachomatis ◽  
Ureaplasma Urealyticum ◽  
Young Patients ◽  
Clinical Importance ◽  
Mycoplasma Hominis ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Cross Sectional ◽  
Urogenital Infection ◽  
Urogenital Infections

Abstract Female and male infertility have been associated to Chlamydia trachomatis, Ureaplasma urealyticum and Mycoplasma hominis urogenital infections. However, evidence from large studies assessing their prevalence and putative associations in patients with infertility is still needed. The study design was a cross-sectional study including 1554 female and 3610 male patients with infertility in Cordoba, Argentina. In women, the prevalence of C. trachomatis, U. urealyticum and M. hominis urogenital infection was 4.3%, 31.1% and 12.1% whereas in men it was 5.8%, 19.2% and 5.3%, respectively. C. trachomatis infection was significantly more prevalent in men whereas U. urealyticum and M. hominis infections were more prevalent in women. Besides, C. trachomatis and U. urealyticum infections were significantly higher patients younger than 25 years. Moreover, U. urealyticum and M. hominis infections were associated to each other in either infertile women or men. Our data revealed that C. trachomatis, U. urealyticum and M. hominis are prevalent uropathogens in infertile patients. Of clinical importance, C. trachomatis and U. urealyticum infections were more prevalent in young patients whereas U. urealyticum and M. hominis are reciprocal risk factors of their co-infection. These results highlight the importance of including the screening of urogenital infections in the diagnostic workup of infertility.

Resistance to tetracyclines among clinical isolates of Mycoplasma hominis and Ureaplasma species: a systematic review and meta-analysis

2020 ◽  
Author(s):  
Mohammad H Ahmadi
Keyword(s):  
Publication Bias ◽  
Antibiotic Susceptibility ◽  
Ureaplasma Urealyticum ◽  
Meta Analysis ◽  
Mycoplasma Hominis ◽  
P Value ◽  
Ureaplasma Parvum ◽  
Urogenital Infections ◽  
Global Status ◽  
High Level

Abstract Background Resistance to tetracyclines, the first-line treatment for urogenital infections caused by Mycoplasma hominis and Ureaplasma species, is increasing worldwide. The aim of the present study was to determine the global status of resistance to this class of antibiotics. Methods Electronic databases were searched using keywords including ‘Mycoplasma’, ‘Mycoplasma hominis’, ‘M. hominis’, ‘Ureaplasma’, ‘Ureaplasma urealyticum’, ‘Ureaplasma parvum’, ‘U. urealyticum’, ‘U. parvum’, ‘Ureaplasma species’, ‘resistance’, ‘antibiotic resistance’, ‘antibiotic susceptibility’, ‘antimicrobial resistance’, ‘antimicrobial susceptibility’, ‘tetracycline’, ‘doxycycline’ and ‘minocycline’. Finally, after some exclusions, 37 studies from different countries were included in the study and meta-analysis was performed on the data collected. Results The midrange resistance rates for M. hominis and U. urealyticum/parvum to tetracycline, doxycycline and minocycline were 50.0%, 9.0% and 16.7% and 43.3%, 28.6% and 9.0%, respectively. A high level of heterogeneity was observed in all studies (I2 &gt; 50%, P value &lt; 0.05), except those representing doxycycline resistance in M. hominis isolates (I2 = 39.1%, P  = 0.02). No evidence of publication bias was observed in the studies and neither Egger’s test nor Begg’s test showed significant publication bias. Conclusions The results of the present study show that the overall resistance to tetracyclines is relatively high and prevalent among M. hominis and Ureaplasma species throughout the world. This highlights the importance of and necessity for regional and local antibiotic susceptibility testing before treatment choice as well as development of newer generations of tetracyclines to prevent antibiotic misuse, emergence and spread of resistant strains and, finally, the failure of treatment.

scholarly journals Features of the course of syphilis in HIV infected patients

2020 ◽  
Vol 96 (1) ◽  
pp. 52-57
Author(s):  
S. V. Koshkin ◽  
A. L. Evseeva ◽  
V. V. Ryabova ◽  
O. S. Kovrova
Keyword(s):  
Hiv Infection ◽  
Clinical Picture ◽  
Mycoplasma Hominis ◽  
Mycoplasma Genitalium ◽  
Risk Groups ◽  
Hiv Positive ◽  
Urogenital Infections ◽  
Viral Hepatitis B ◽  
The Russian Federation ◽  
Ureaplasma Spp

In the present article, relevant data on the characteristics of the course of infections transmitted through sexual contact (hereinafter STIs) are considered at the present stage. Particular attention is paid to mixed infections, in particular the combination of syphilis and HIV. Since the presence of both infections in a patient simultaneously significantly complicates the clinical picture, and also contributes to their spread in the population, which is an acute social problem. The article presents modern data on the epidemiology, level and dynamics of the incidence of syphilis and HIV infection in the Russian Federation, including among high-risk groups. Research data on the incidence of STIs (urogenital infections caused by Mycoplasma genitalium, Ureaplasma spp., Mycoplasma hominis, Chlamydia trachomatis, syphilis) and viral hepatitis B and C in 60 HIV-positive prisoners in the Kirov region are presented. The features of the development and course of syphilitic infection, the difficulties of diagnosis and therapy that occur with HIV are described. A case of own clinical observation of the malignant course of syphilis associated with HIV infection is presented. Both infections have been identified in the hospital where the patient is admitted with a diagnosis of spreading pyoderma, exhibited on the basis of complaints and clinical picture (ektimatoznye rash).

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