Antioxidant activity and phytochemical screening of the methanol extracts of Euphorbia hirta L

Euphorbia hirta L (Euphorbiaceae) also called asthma herb has long been prescribed in traditional medicine because it exhibits diverse pharmacological actions due to the presence of alkaloids, flavonoids, polyphenols, triterpenoids, and saponins. The present study is aimed at the study of phytochemical and antioxidant activity and anti-inflammatory screening of E. hirta. Extraction of dried powder was performed followed by phytochemical screening using color reactions. Total phenolic content (TPC) and total flavonoid content (TFC) of the extracts were estimated by Folin-Ciocalteu and Aluminum chloride method respectively. The antioxidant activity was studied by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging method. Anti-inflammatory activity was studied by using protein denaturation in vitro bioassay. Phytochemical screening showed the presence of flavonoids, alkaloids, and phenolic compounds. TPC, TFC and antioxidant activity (IC50) of the extract were found as 288.10 mg gallic acid equivalent per gram (GAE/g), 29.36 mg quercetin equivalent per gram (QE/g) and 32.23 µg/mL (p<0.05) respectively. Diclofenac sodium and E. hirta extract showed the maximum inhibition of 91.28% and 68.20% respectively at the concentration of 1000 µg/mL compared with control (p>0.05). The phenolic compounds and flavonoids exert antioxidant and anti-inflammatory activities because of their scavenging ability. The demonstrated antioxidant and anti-inflammatory activities may be the rationale behind some of its folkloric uses and also may be responsible for some of its pharmacological effects. Thus, E. hirta can be considered a good source of antioxidants and anti-inflammatory actions, which might be beneficial for combating oxidative stress.

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In vitro antioxidant activity and qualitative phytochemical analysis of two Vismia (Hypericaceae) species collected in Los Andes, Venezuela

Revista de Biología Tropical ◽

10.15517/rbt.v64i4.22968 ◽

2016 ◽

Vol 64 (4) ◽

Author(s):

Alexis Buitrago ◽

Janne Del Carmen Rojas ◽

Yonel Peñalosa

Keyword(s):

Antioxidant Activity ◽

Radical Scavenging ◽

Total Phenolic ◽

Phytochemical Analysis ◽

Phytochemical Screening ◽

Methanol Extracts ◽

Phenol Derivatives ◽

Methanolic Extracts ◽

High Concentrations

Vismia genus is distributed mainly in tropical and subtropical regions of Central, South America and some areas of Africa. According to previous investigations, antioxidant potential of Vismia species might be related to anthrones, anthraquinones, flavonoids and phenol derivatives biosynthesized by these plants. The aim of present study is to evaluate the free radical scavenging capacity, total phenolic and flavonoids content as well as the qualitative phytochemical screening of methanol extracts obtained from Vismia macrophylla (VM) and Vismia baccifera (VB) collected in Táchira and Mérida state, Venezuela. Phytochemical screening of VB and VM methanolic extracts carried out using various chemical assays revealed an abundant presence of anthraquinones in both species analyzed. Glycosides were also present while flavones and dehydroflavones were observed abundantly in VB but moderated in VM. Triterpenes were also detected and steroids showed to be abundant in VM but moderate in VB. On the other hand, antioxidant capacity measured by the DPPH radical scavenging assay showed that VM possesses a stronger antioxidant activity than VB with IC50 5.50 µg/mL. Phenol and flavonoid assays also revealed that methanol extracts of both VM and VB contain high concentrations of these metabolites. A relationship between the antioxidant activity, total phenol and flavonoids content of the extracts analyzed was demonstrated in present investigation since those samples with higher phenolic concentrations showed likewise higher antioxidant activity.

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Phytochemical Screening, Cytotoxic and Antioxidant Activity of Alternathera sessilis and Moringa oleifera

Amrit Research Journal ◽

10.3126/arj.v1i1.32456 ◽

2020 ◽

Vol 1 (1) ◽

pp. 65-71

Author(s):

Ishwor Pathak ◽

Rabin Budhathoki ◽

Nisha Yadav ◽

Muna Niraula ◽

Surya Kant Kalauni

Keyword(s):

Antioxidant Activity ◽

Brine Shrimp ◽

Moringa Oleifera ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Phytochemical Screening ◽

Methanol Extracts ◽

Aerial Parts ◽

Ic50 Value ◽

Hexane Extracts

Methanol and hexane extracts of leaves of Moringa oleifera and aerial parts of Alternanthera sessilis were screened for the presence of different classes of phytoconstituents. Phytochemical screening revealed the presence of alkaloids, flavonoids, carbohydrates, terpenoids, polyphenols, glycosides, and coumarins in methanol extracts. Volatile oils, quinines, and phytosterols were absent in all extracts and saponins were present in all extracts. The biological activity of all the extracts was tested by performing brine shrimp bioassay. All the extracts except hexane extract of Moringa oleifera were found to be cytotoxic against brine shrimp nauplii. 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay was used to evaluate the antioxidant activity of the extracts. Methanol extracts of Moringa oleifera showed the strongest antioxidant activity with an IC50 value of 65.77 μg/mL. Regarding the ascorbic acid (IC50 value 39.53 μg/mL) as standard, methanol extracts of both plants showed high free radical scavenging activity than that of hexane extracts.

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Evaluation of phytochemical, antioxidant, and In-vitro antidiarrhoeal, activity of Euphorbia hirta

Journal of Drug Delivery and Therapeutics ◽

10.22270/jddt.v9i1-s.2352 ◽

2019 ◽

Vol 9 (1-s) ◽

pp. 290-294

Author(s):

Suhail Ahmad Mir ◽

Mudasir Maqbool ◽

Khursheed Ahmad Sheikh ◽

Ahsan Ullaha Bhat ◽

Ghulam Nabi Bader

Keyword(s):

Antioxidant Activity ◽

Extraction Methods ◽

Salmonella Typhi ◽

Diffusion Method ◽

Phytochemical Screening ◽

Euphorbia Hirta ◽

Phytochemical Constituents ◽

Agar Diffusion Method ◽

Antidiarrheal Activity

Background: The current study was carried out to evaluate the phytochemical, antioxidant, and In-vitro antidiarrheal properties of Euphorbia hirta Methods: Extracts were obtained using cold extraction, hot extraction and autoclave extraction methods using Methanol, Chloroform, Petroleum ether and distilled water as solvents. Crude extracts were screened for different phytochemical constituents like sugars, saponins, flavonoids, tannins, and glycosides etc. Antioxidant activity was evaluated using spectrophotometric method. The in-vitro antidiarrheal activity was elucidated by the antimicrobial activity using agar diffusion method. Results: Methanol proved to be a good solvent for extraction. In-vitro antidiarrheal activity was shown by all extracts on Escherichia coli, Staphylococcus aureus, and Salmonella typhi bacteria which are mainly responsible for diarrhea. Conclusion: From the phytochemical screening it shows presence of phytochemicals like alkaloids, tannins, glycosides, and saponins. The plant also shows antioxidant activity, methnolic extracts shows higher activity and it shows In-vitro antidiarrhoeal activity which clearly indicates that the plant can be used for the treatment of diarrhea. Further studies should be done to isolate the compound responsible for activity in the experimental animals. Keywords: Euphrobia hirta, autoclave extraction, antidiarrheal, phytochemicals.

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Phytochemical screening and antioxidant activities of Methanol extracts from eight Syzygium species

Malaysian Journal of Fundamental and Applied Sciences ◽

10.11113/mjfas.v16n1.1393 ◽

2020 ◽

Vol 16 (1) ◽

pp. 13-17

Author(s):

Siti Awanis Abdullah ◽

Shajarahtunnur Jamil

Keyword(s):

Antioxidant Activity ◽

Plant Extracts ◽

Antioxidant Activities ◽

Ferric Ion ◽

Phytochemical Screening ◽

Abts Assay ◽

Methanol Extracts ◽

Crude Extracts ◽

Dpph Scavenging Activity ◽

Dpph Scavenging

This study was carried out to investigate the phytochemical screening of the methanol crude extracts of the leaves, twigs, and heartwoods of eight Syzygium species (S. filiforme var. filiforme, S. papillosum, S. griffithii, S. pseudoformosum, S. claviflorum var. claviflorum, S. glaucum, S. syzygioides, and Eugenia Sp. 57). The analysis revealed the presence of alkaloids, flavonoids, phenols, steroids, terpenes, and tannins except saponins in all plant extracts. Evaluation of the antioxidant activities were tested using DPPH, FRAP, and ABTS assays. All plant extracts showed good DPPH scavenging activity with the percentage inhibition more than 70 % at 100 ppm. The leaves and twigs of the methanol extracts of S. griffithii exhibited the most potent ferric ion reducer ranging from 1.03 ± 0.03 mM to 7.52 ± 0.49 mM and 1.43 ± 0.12 mM to 9.68 ± 0.38 mM FRAP equivalent, respectively. The heartwoods crude extract of S. syzygioides showed the most potent ferric ion reducer ranging from 1.20 ± 0.01 mM to 10.47 ± 0.12 mM FRAP equivalent. All of the leaves crude extracts showed potential antioxidant activities towards ABTS assay. The methanol extracts of the leaves of S. glaucum showed good SC50 value (116.90 mM) followed by S. papillosum (S2LM, SC50 value = 132.70 mM) and Eugenia Sp. 57 (S4LM, SC50 value = 143.10 mM). The twigs crude extracts of S. syzygioides (S8TM) and S. griffithii (S3TM) showed excellent antioxidant activity towards ABTS assay with the SC50 values of 130.60 mM and 145.40 mM, respectively. The crude extracts of S. syzygioides exhibited good antioxidant activity among the heartwoods extracts with the SC50 value of 96.20 mM.

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UJI AKTIVITAS ANTIOKSIDAN FRAKSI N-HEKSAN DAUN AFRIKA (Vernonia amygdalina Del.) DENGAN METODE DPPH

Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan ◽

10.36387/jiis.v5i2.466 ◽

2020 ◽

Vol 5 (2) ◽

pp. 250-257

Author(s):

Nurul Fatimah ◽

◽

Reksi Sundu

Keyword(s):

Oxidative Stress ◽

Antioxidant Activity ◽

Free Radicals ◽

Ethanolic Extract ◽

Ethanol Extract ◽

Hexane Fraction ◽

Phytochemical Screening ◽

Vernonia Amygdalina ◽

Tropical Africa ◽

Ic50 Value

Free radicals and reactive species are widely believed to contribute to the development of several diseases by causing oxidative stress and eventually oxidative. Vernonia amygdalina (Astereacea) is a small shrub or tree between 1 and 5m high growing throughout tropical Africa. Plants are generally known as bitter leaves is well cultivated and is a general market for merchandise in several countries. The purpose of this study was to determine the antioxidant activity of hexane fraction from ethanol extract od Frican leaves (Vernonia amygdalina Del.). The method used in this study was the DPPH (1,1-Diphenil-2-Picrylhydrazyl) method. The result of phytochemical screening showed that ethanolic extract of African leaves contained a composition of secondary metabolites of alkaloids, flavonoids, tannins, steroids/triterpenoids and saponins. The antioxidant activity of the extract of n-hexane fraction was classified as very weak with an IC50 value of 317.98 ppm.

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Antioxidant Activity in Water and Methanol Extracts from Korean Edible Wild Plants

Journal of the Korean Society of Food Science and Nutrition ◽

10.3746/jkfn.2011.40.1.029 ◽

2011 ◽

Vol 40 (1) ◽

pp. 29-36 ◽

Cited By ~ 45

Author(s):

Young-Min Lee ◽

Ji-Hyun Bae ◽

Ho-Young Jung ◽

Jae-Hyun Kim ◽

Dong-Sik Park

Keyword(s):

Antioxidant Activity ◽

Wild Plants ◽

Methanol Extracts ◽

Edible Wild Plants

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Evaluation of Some Biological Applications of Pleurotus citrinopileatus and Boletus edulis Fruiting Bodies

Current Pharmaceutical Biotechnology ◽

10.2174/1389201020666190904162403 ◽

2019 ◽

Vol 20 (15) ◽

pp. 1309-1320 ◽

Cited By ~ 1

Author(s):

Ahmed M. Younis ◽

Marwa M. Abdel-Aziz ◽

Mohamed Yosri

Keyword(s):

Antioxidant Activity ◽

Flow Cytometric Analysis ◽

Radical Scavenging ◽

Biologically Active ◽

Fruiting Bodies ◽

Methanol Extracts ◽

Boletus Edulis ◽

Glucosidase Activity ◽

Hela Cell Lines ◽

Pleurotus Citrinopileatus

Background:: Mushrooms are deemed as a special delicacy in many countries. They are considered an important cuisine due to their bioactive ingredients and possible health benefits. Methods: Herein, we measured selected biological properties of methanol extracts of Pleurotus citrinopileatus and Boletus edulis fruiting bodies including; in vitro antimicrobial activity, anti-α- glucosidase activity, antioxidant activity, anti-lipase activity and cytotoxic activity against different cancer cells and normal cells. Results: B. edulis methanol extracts showed high antimicrobial and anti-α-glucosidase activity. In contrast, P. citrinopileatus methanol extracts showed superior antioxidant activity indicated by (1,1- diphenyl-2-picrylhydrazyl) DPPH radical scavenging with half maximal inhibitory concentration of IC50 37.4 µg/ml, anti-lipase activities with IC50 65.2 µg/ml and high cytotoxicity activity against HepG2 and HeLa cell lines with IC50 22.8 and 36.7 µg/ml, respectively. Flow cytometric analysis of the cell cycle was used to show apoptotic effects of methanol extracts against HepG2 and HeLa cells. Conclusion: P. citrinopileatus and B. edulis methanolic extracts appear to contain biologically active compounds that might be used to treat some common human diseases.

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Antihyperglycemic, Antihyperlipidemic and Antioxidant Effects of Cotula cinerea (Del) in Normal and Streptozotocin-Induced Diabetic Rats

Endocrine Metabolic & Immune Disorders - Drug Targets ◽

10.2174/1871530320666200513081312 ◽

2020 ◽

Vol 20 (9) ◽

pp. 1504-1513 ◽

Cited By ~ 1

Author(s):

Ayoub Amssayef ◽

Mohamed Eddouks

Keyword(s):

Antioxidant Activity ◽

Glucose Tolerance ◽

Oral Administration ◽

Beneficial Effect ◽

Aqueous Extract ◽

Aerial Part ◽

Diabetic Rats ◽

Histopathological Analysis ◽

Phytochemical Screening ◽

Cotula Cinerea

Aims: The current investigation aimed to assess the antioxidant, antidiabetic and antilipidemic effects of the aqueous extract of aerial part of Cotula cinerea (C. cinerea). Background: Cotula cinerea (Del). which belongs to the Asteraceae family is commonly used traditionally for the treatment of diabetes. Objective: The objective of the study was to study the effect of the aqueous C. cinerea extract on glucose and lipid metabolism in normal and streptozotocin-induced diabetic rats using a single and repeated oral administration. Methods: A preliminary phytochemical screening and the quantification of phenolic and flavonoid contents as well as the antioxidant activity using three methods (DPPH, FRAP and ABTS) were carried out. The effect of a single and repeated (15 days of treatment) oral administration of the aqueous extract of aerial part of Cotula cinerea (AEAPCC) at a dose of 20 mg/kg on glucose and lipid profile was examined in normal and streptozotocin-induced diabetic rats. Additionally, histopathological examination of the pancreas and liver was carried out according to the Hematoxylin-Eosin method. Results: AEAPCC (20 mg/kg) showed a significant blood glucose-lowering activity in both normal and diabetic rats after a single and repeated oral administration during 15 days. The aqueous extract was also able to decrease the plasma triglycerides levels in both normal and diabetic rats after 15 days of oral treatment at a dose of 20 mg/Kg while no effect was observed on plasma cholesterol levels. In addition, the results show that AEAPCC exhibits an in vitro antioxidant activity using different tests. Histopathological analysis of the pancreas and liver of AEAPCC-treated diabetic rats has revealed that AEAPCC had a beneficial effect on the architecture of these organs while no improvement of glucose tolerance was noticed using the glucose tolerance test. Furthermore, the results showed that the extract is rich in several phytochemical compounds and exhibited an important antioxidant activity. The phytochemical screening revealed that AEAPCC contains polyphenolic compounds, flavonoids, tannins, alkaloids, saponins, quinones, sterols, terpenoids, anthroquinones and reducing sugars. Whereas, it is free from glycosides. Conclusion: In conclusion, this study demonstrates that Cotula cinerea possesses a beneficial effect on diabetes. Further investigations are required to study the mechanism of action of the antidiabetic effect of this plant.

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