The influence of apple- and red-wine pomace rich diet on mRNA expression of inflammatory and apoptotic markers in different piglet organs

2006 ◽  
Vol 82 (6) ◽  
pp. 877-887 ◽  
Author(s):  
J. Sehm ◽  
H. Lindermayer ◽  
H. H. D. Meyer ◽  
M. W. Pfaffl
Keyword(s):  
Gene Expression ◽  
Cell Cycle ◽  
Mrna Expression ◽  
Caspase 3 ◽  
Red Wine ◽  
Marker Genes ◽  
Apoptotic Marker ◽  
Mrna Gene ◽  
Turn Over ◽  
Mrna Gene Expression

Flavan-3-ols are a class of flavonoids that are widely distributed in fruits and beverages including red wine and apples. Consumption of flavanoid-rich food has been shown to exhibit anti-microbial, anti-oxidative, anti-inflammatory, and immune-modulating effects. To test the nutritional effects of flavanols on mRNA gene-expression of inflammatory and apoptotic marker genes, piglets were given two flavanoids-rich feeding regimens: a low flavanoid standard diet (SD) was compared with diets enriched with 3·5% apple pomace (APD) or 3·5% red-wine pomace (RWPD). The influence on mRNA expression levels was investigated in different immunological active tissues and in the gastro-intestinal tract (GIT). The investigation took place from 1 week prior weaning to 19 days post weaning in 78 piglets. The expression of expressed marker genes was determinate by one-step quantitative real-time (qRT-PCR): TNFα, NFκB as pro-inflammatory; IL10, as anti-inflammatory; caspase 3 as apoptosis; cyclin D1 as cell cycle marker; and nucleosome component histon H3 as reference gene.The feeding regimens result in tissue individual regulation of mRNA gene expression in all investigated organs. It was discovered that there were significant differences between the applied diets and significant changes during feeding time curse. Both pomace treatments caused a significant up-regulation of all investigated genes in liver. The effect on mesenterial lymph nodes and spleen was not prominent. In the GIT, the treatment groups showed a inhibitory effects on gene expression mainly in stomach and jejunum (NFκB, cyclin D1 and caspase 3). In colon the trend of caspase 3 was positive with the greatest change in the RWPD group.In jejunum and stomach the cell cycle turn over was reduced, whereas in liver the cell turn over was highly accelerate. The influence on inflammatory marker gene expression is mainly relevant in stomach. It is presume that both flavanoid rich feeding regimens have the potential to modulate the mRNA expressions of inflammatory, proliferation and apoptotic marker genes in the GIT and piglet organs.

scholarly journals THE EFFECT OF ADMINISTRATION OF ETHANOL EXTRACT FROM MUSA PARADISIACA L. (MPL) FRUIT ON THE CASPASE-3 MRNA GENE EXPRESSION IN RAT AMYLOID BETA INDUCED, AN ALZHEIMER’S DISEASE MODEL

2018 ◽  
Vol 11 (4) ◽  
pp. 298 ◽  
Author(s):  
Emma Kamelia ◽  
Andi Asadul Islam ◽  
Mochammad Hatta ◽  
Cahyono Kaelan ◽  
Ilhamjaya Patellongi
Keyword(s):  
Gene Expression ◽  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Mrna Expression ◽  
Amyloid Beta ◽  
Caspase 3 ◽  
Ethanol Extract ◽  
Musa Paradisiaca ◽  
Mrna Gene ◽  
Mrna Gene Expression

 Objective: The objective of this study is to analyze caspase-3 mRNA gene expression in a Wistar rat model of Alzheimer’s disease (AD) treated with Musa paradisiaca L. (MPL) ethanol extract or banana extract (BE)Methods: MPL was evaluated for its effect on the caspase-3 mRNA gene expression of rat amyloid beta (Aβ) induced, an AD model. Each model included twenty Wistar rats were randomized into five groups: K0, without AD induction and no BE; K1, AD induction and no BE; K2, AD induction+BE 250 mg/kg body weight (BW); K3, AD induction+BE 500 mg/kg; and K4, AD induction+BE 1000 mg/kg. AD induction was performed by Aβ1-42 (0.2 ug) injection at the intracerebroventricular area. mRNA caspase-3 level measurements were performed by real time-polymerase chain reaction.Results: Paired t-test analysis showed no significant differences of caspase-3 mRNA level before Aβ induction among five groups (p>0.05). At 6 weeks post-Aβ induction, there was significantly increased caspase-3 mRNA in all groups except K0 (p<0.05). Notably, after 3 weeks of BE administration, caspase-3 mRNA expression was significantly decreased in all BE-treated groups; in the K1 placebo group, caspase-3 mRNA expression increased. The maximum decreased caspase-3 mRNA expression was in group K4 (-BE 1000 mg/kg BW), and the minimum was in group K2 (-BE 250 mg/kg BW).Conclusion: The results revealed that the ethanolic extract of MPL fruit could decrease caspase-3 mRNA gene expression in AD rat.

scholarly journals Over, and Underexpression of Endothelin 1 and TGF-Beta Family Ligands and Receptors in Lung Tissue of Broilers with Pulmonary Hypertension

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Norma Dominguez-Avila ◽  
Gabriel Ruiz-Castañeda ◽  
Javier González-Ramírez ◽  
Nora Fernandez-Jaramillo ◽  
Jorge Escoto ◽  
...  
Keyword(s):  
Gene Expression ◽  
Pulmonary Hypertension ◽  
Mrna Expression ◽  
Cardiac Failure ◽  
Transforming Growth Factor Beta ◽  
Transforming Growth Factor ◽  
Control Group ◽  
Endothelin 1 ◽  
Mrna Gene ◽  
Mrna Gene Expression

Transforming growth factor beta (TGFβ) is a family of genes that play a key role in mediating tissue remodeling in various forms of acute and chronic lung disease. In order to assess their role on pulmonary hypertension in broilers, we determined mRNA expression of genes of the TGFβfamily and endothelin 1 in lung samples from 4-week-old chickens raised either under normal or cold temperature conditions. Both in control and cold-treated groups of broilers, endothelin 1 mRNA expression levels in lungs from ascitic chickens were higher than levels from healthy birds (), whereas levels in animals with cardiac failure were intermediate. Conversely, TGFβ2 and TGFβ3 gene expression in lungs were higher in healthy animals than in ascitic animals in both groups (). TGFβ1, TβRI, and TβRII mRNA gene expression among healthy, ascitic, and chickens with cardiac failure showed no differences (). BAMBI mRNA gene expression was lowest in birds with ascites only in the control group as compared with the values from healthy birds ().

Achras Zapota L Reduce Expression Of mRNA High Motility Group Box 1 (HMGB1)

2020 ◽  
Vol 16 ◽  
Author(s):  
Hasta Handayani Idrus ◽  
Mochammad Hatta ◽  
Veny Hadju ◽  
Suryani As'ad ◽  
Vivien Novarina Kasim ◽  
...  
Keyword(s):  
Gene Expression ◽  
Typhoid Fever ◽  
Mrna Expression ◽  
Extraction Method ◽  
Salmonella Typhi ◽  
Fruit Extract ◽  
Palm Fruit ◽  
Mrna Gene ◽  
Almost All ◽  
Mrna Gene Expression

Background: Manila sapodilla fruit (Achras zapota L) has been widely known empirically in the community, namely fruit used for the treatment of typhoid fever by means of raw fruit in washing / cleaning then the fruit is shredded and the result of grated squeezed using fine leaves and the filter is drunk in patients with typhoid fever. High Motility Group Box 1 (HMGB1) is a protein that is expressed constitutively in almost all types of cells. Objective: To determine the effect of Manila Palm fruit extract (Achras Zapota L) on the expression of the High Motility Group Box 1 (HMGB1) gene in mice infected with Salmonella typhi. Methods: The method used for extracting sapodilla fruit is the maceration method and the tool used to see changes in HMGB1 mRNA gene expression is PCR with boom extraction method. Conclusion: Some sapodilla manila fruit extract dose 510 mg / KgBB (p = 0.016, p <0.05) (∆ mean = 1.90) and 750 mg / KgBB (p = 0.000, p <0.05) (∆ mean = 4.19), can decrease HMGB1 mRNA expression was observed at day 4, 10 to day 30 with no effect with levofloxacin 98 mg / KgBB (p = 0.000, p <0.05) (∆ mean = 5.97).

Evaluation of Caspase-3 mRNA Gene Expression Activity in Amyloid Beta-induced Alzheimer’s Disease Rats

2017 ◽  
Vol 17 (3) ◽  
pp. 117-125 ◽  
Author(s):  
Emma Kamelia ◽  
Andi Asadul Isl ◽  
Mochammad Hatta ◽  
Cahyono Kaelan ◽  
Ilhamjaya Patellongi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Amyloid Beta ◽  
Caspase 3 ◽  
Mrna Gene ◽  
Expression Activity ◽  
Mrna Gene Expression

scholarly journals Lactulose: effect on apoptotic- and immunological-markers in the gastro-intestinal tract of pre-ruminant calves

2008 ◽  
Vol 52 (No. 10) ◽  
pp. 437-444 ◽  
Author(s):  
S. Fleige ◽  
W. Preißnger ◽  
H.H.D. Meyer ◽  
M.W. Pfaffl
Keyword(s):  
Gene Expression ◽  
Intestinal Tract ◽  
Caspase 3 ◽  
Fold Increase ◽  
Probiotic Strain ◽  
Treated Group ◽  
Control Group ◽  
High Dose ◽  
Mrna Gene ◽  
Mrna Gene Expression

The study was conducted to elucidate the effects of orally administered lactulose in combination with <i>Enterococcus faecium</i> on immune response of the intestinal tract in pre-ruminant calves. The mRNA expression of pro- and anti-inflammatory cytokines and proliferation and apoptosis markers were investigated in jejunum, ileum, colon and caecum. Simmental calves were fed diets containing 1% (L1) or 3% (L3) lactulose and the probiotic strain of the genus <i>E. faecium</i>, and compared with a non treated control group. Primarily the high dose feeding with lactulose showed an effect on several mRNA gene expression parameters. In the jejunum a down-regulation of the anti-apoptotic marker Bcl-xl was determined and IL-10 mRNA gene expression was 2.6-fold up-regulated (<i>P</i> < 0.05). In the colon a 1.9-fold (<i>P</i> < 0.05) up-regulation of IL-10 and only in caecum an about 2-fold increase of TGF-β1 (<i>P</i> < 0.05) was found for both lactulose feedings. Caspase 3 was up-regulated in caecum only in the 3% lactulose treated group (<i>P</i> < 0.05). The enhanced apoptotic rate of caspase 3 seems to be associated with a decrease in crypth depth due to lactulose supplementation. The results indicated that mainly the high 3% lactulose dose in probiotic-fed calves has an affect on the intestinal immune function and on diverse apoptotic markers.

scholarly journals Quantitative RT-PCR analyses of five evolutionary conserved genes in Alligator brains during development

2011 ◽  
Vol 2 (4) ◽  
Author(s):  
Sarah Wilson ◽  
Tianli Zhu ◽  
Rajesh Khanna ◽  
Michael Pritz
Keyword(s):  
Gene Expression ◽  
Brain Area ◽  
Brain Regions ◽  
Adult Brain ◽  
Time Interval ◽  
Conserved Genes ◽  
Mrna Gene ◽  
Alligator Mississipiensis ◽  
Mrna Gene Expression ◽  
Present Gene

AbstractGene expression was investigated in the major brain subdivisions (telencephalon, diencephalon, midbrain and hindbrain) in a representative reptile, Alligator mississipiensis, during the later stages of embryonic development. The following genes were examined: voltage-gated sodium channel isoforms: NaV1.1 and NaV1.2; synaptic vesicle 2a (SV2a); synaptophysin; and calbindin 2. With the exception of synaptophysin, which was only expressed in the telencephalon, all genes were expressed in all brain regions sampled at the time periods examined. For NaV1.1, gene expression varied according to brain area sampled. When compared with NaV1.1, the pattern of NaV1.2 gene expression differed appreciably. The gene expression of SV2a was the most robust of any of the genes examined. Of the other genes examined, although differences were noted, no statistically significant changes were found either between brain part or time interval. Although limited, the present analysis is the first quantitative mRNA gene expression study in any reptile during development. Together with future experiments of a similar nature, the present gene expression results should determine which genes are expressed in major brain areas at which times during development in Alligator. When compared with other amniotes, these results will prove useful for determining how gene expression during development influences adult brain structure.

scholarly journals Diet-Induced Obesity Disrupts Trace Element Homeostasis and Gene Expression in the Olfactory Bulb

Nutrients ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 3909
Author(s):  
Melissa S. Totten ◽  
Derek M. Pierce ◽  
Keith M. Erikson
Keyword(s):  
Gene Expression ◽  
Olfactory Bulb ◽  
Trace Element ◽  
Alpha Synuclein ◽  
Divalent Metal Transporter 1 ◽  
Diet Induced Obesity ◽  
Mrna Gene ◽  
The Impact ◽  
Mrna Gene Expression ◽  
Obese Male

The aim of this study was to determine the impact of diet-induced obesity (DIO) on trace element homeostasis and gene expression in the olfactory bulb and to identify potential interaction effects between diet, sex, and strain. Our study is based on evidence that obesity and olfactory bulb impairments are linked to neurodegenerative processes. Briefly, C57BL/6J (B6J) and DBA/2J (D2J) male and female mice were fed either a low-fat diet or a high-fat diet for 16 weeks. Brain tissue was then evaluated for iron, manganese, copper, and zinc concentrations and mRNA gene expression. There was a statistically significant diet-by-sex interaction for iron and a three-way interaction between diet, sex, and strain for zinc in the olfactory bulb. Obese male B6J mice had a striking 75% increase in iron and a 50% increase in manganese compared with the control. There was an increase in zinc due to DIO in B6J males and D2J females, but a decrease in zinc in B6J females and D2J males. Obese male D2J mice had significantly upregulated mRNA gene expression for divalent metal transporter 1, alpha-synuclein, amyloid precursor protein, dopamine receptor D2, and tyrosine hydroxylase. B6J females with DIO had significantly upregulated brain-derived neurotrophic factor expression. Our results demonstrate that DIO has the potential to disrupt trace element homeostasis and mRNA gene expression in the olfactory bulb, with effects that depend on sex and genetics. We found that DIO led to alterations in iron and manganese predominantly in male B6J mice, and gene expression dysregulation mainly in male D2J mice. These results have important implications for health outcomes related to obesity with possible connections to neurodegenerative disease.

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