A note on the protein content of the empty body of young Polish Black-and-White Lowland bulls

1972 ◽  
Vol 14 (1) ◽  
pp. 119-122 ◽  
Author(s):  
Zofia Osińska ◽  
Aleksandra Ziołecka
Keyword(s):  
Protein Content ◽  
Total Protein ◽  
Live Weight ◽  
Total Protein Content ◽  
Weight Range ◽  
Black And White ◽  
Edible Parts

SUMMARYData on the protein content of the empty body were obtained for 44 young Polish Black-and-White Lowland bulls ranging from 10-day- old calves to bulls more than 400 kg live weight. The percentage of protein of the empty body was found to be practically constant (19–19·5%) over the weight range investigated. Less than a half (up to 48 %) of the total protein content was found in the edible parts of the carcass.

Estimation of protein, chemical fat and energy content in pigs

1962 ◽  
Vol 4 (3) ◽  
pp. 391-398 ◽  
Author(s):  
Zofia Osińska
Keyword(s):  
Body Weight ◽  
Total Protein ◽  
Energy Content ◽  
Live Weight ◽  
Total Protein Content ◽  
Carcass Quality ◽  
Chemical Analyses ◽  
Large White ◽  
Body Components ◽  
Edible Parts

Complete chemical analyses of all body components of 20 Large White pigs were carried out. At the average live-weight of 90·3 kg. (empty body weight 86·5 kg.) the total protein content was found to be 12·7 kg. and that of the chemical fat 25·4 kg. The edible parts of the carcass (i.e. lean and separable fat) contained only 58·5% (range 55·2–61·3) of the total protein but as much as 85% of the total chemical fat. Simple and multiple correlations were computed between various carcass characters and protein, chemical fat and energy content. The results indicate that it may be possible to obtain fairly accurate estimates of the chemical composition and energy content of the edible parts of the carcass, of the whole carcass, and of the empty body by methods based on various systems of assessing the carcass quality.

HEMATOLOGICAL PARAMETERS PUREBRED AND CROSSBRED CALVES

2016 ◽  
Vol 1 (3) ◽  
pp. 45-48
Author(s):  
Исхаков ◽  
Rishat Iskhakov
Keyword(s):  
Protein Content ◽  
Total Protein ◽  
Blood Cells ◽  
Hematological Parameters ◽  
Physiological State ◽  
White Blood Cells ◽  
The Body ◽  
Adaptive Plasticity ◽  
Total Protein Content ◽  
Black And White

The purpose of research is scientifically and practically prove the possibility of increasing the level of meat productivity purebred for young calves at the crossing of Black-motley cows with bulls of the breed Obrac. Research objects are the bulls of Black-motley breed and their hybrids I generation with Obrac breed. For the experience 4 groups of animals were formed: I and III – Black and white breed, II and IV – gobies Оbrac crossbreeds ½ x ½ black-and-white. Bulls of groups III and IV in the 2 months of age were castrated by open way. All experimental animals identical conditions of detention and feed have been established. To monitor the physiological state of an organism of young animals in the winter and in the blood of the summer, blood was taken from the jugular vein before feeding and watering, measured hemoglobin, alkaline reserve, the number of white blood cells, red blood cells, serum – total protein, protein fractions, calcium, phosphorus, , the activity of AST and ALT. Deviations from the physiological norm morphological parameters of blood in the experimental groups of calves during the experiment was not set. Total protein content in serum was higher in summer compared to winter. Increasing the total protein content have purebred calves was 4.1 g/l (5.3%), crossbred steers 3.3 g/l (4.2%), respectively castrates at 4.2 g/l (5.6%) and 4.9 g/l (6.5%). There is a trend increase of enzyme activity in the summer, due to a more active course of metabolism in the body during this season of the year. Purebred and crossbred young are characterized by high adaptive plasticity, as evidenced by morphological and biochemical indices of blood. All quantitative and qualitative changes in the blood were largely seasonal in nature and due to the influence of environmental conditions. Thus all interior change indicators do not go beyond the limits of the physiological norm.

Prediction of Human Acute Toxicity by the Hep G2/24-hour/Total Protein Assay, with Protein Measurement by the CBQCA Method

2005 ◽  
Vol 33 (3) ◽  
pp. 207-213 ◽  
Author(s):  
Paul J. Dierickx
Keyword(s):  
Protein Content ◽  
Total Protein ◽  
Total Protein Content ◽  
Human Toxicity ◽  
Hep G2 ◽  
Vitro Assay ◽  
Protein Assay ◽  
Lowry Method ◽  
Total Protein Assay

In our previously described Hep G2/24-hour/total protein assay, protein levels were measured by using the Lowry method. This assay was the best acute in vitro assay for the prediction of human toxicity within the Multicentre Evaluation of In Vitro Cytotoxicity (MEIC) study. In order to increase the MEIC data-base with a wider range of chemicals, we were interested in introducing the more practical 3-(4-carboxybenzoyl)-quinoline-2-carboxaldehyde (CBQCA) method for the quantification of the total protein content. Therefore, we investigated whether the same good results for the prediction of acute human toxicity would be obtained with the CBQCA method. The cells were treated for 24 hours, then cytotoxicity was determined by measuring the total protein content with CBQCA. The results were quantified by using the PI50c: the concentration (in mM) of test compound required to reduce the total protein content measured with the CBQCA-method by 50% as compared to the control cells. The results were compared with the PI50, the corresponding value when the Lowry method was used. A relatively low correlation was observed between PI50 and PI50c, reflecting the large and unexpected, differences when using the two protein assays. However, when comparing the log PI50c with the human toxicity, a correlation coefficient of r2 = 0.761 ( n = 44) was obtained for exactly the same series of MEIC chemicals. This value is clearly higher than that for the Lowry method ( r2 = 0.695). Compared to the Lowry method originally used, the Hep G2/24-hour/CBQCA total protein assay has the additional important advantage that it can be very easily adapted for large-scale analyses with robotic systems, including the on-line calculation of the results.

Total protein content and protein synthesis within pre-elongation stage bovine embryos

1998 ◽  
Vol 50 (2) ◽  
pp. 139-145 ◽  
Author(s):  
J.G. Thompson ◽  
A.N.M. Sherman ◽  
N.W. Allen ◽  
L.T. McGowan ◽  
H.R. Tervit
Keyword(s):  
Protein Synthesis ◽  
Protein Content ◽  
Total Protein ◽  
Total Protein Content ◽  
Bovine Embryos

scholarly journals Surface topography of hydroxyapatite affects ROS17/2.8 cells response

2002 ◽  
Vol 16 (3) ◽  
pp. 209-215 ◽  
Author(s):  
Adalberto Luiz Rosa ◽  
Márcio Mateus Beloti ◽  
Richard van Noort ◽  
Paul Vincent Hatton ◽  
Anne Jane Devlin
Keyword(s):  
Cell Proliferation ◽  
Protein Content ◽  
Surface Topography ◽  
Cell Morphology ◽  
Total Protein ◽  
Cell Attachment ◽  
Maxillofacial Surgery ◽  
Total Protein Content ◽  
Alp Activity ◽  
Powder Pressing

Hydroxyapatite (HA) has been used in orthopedic, dental, and maxillofacial surgery as a bone substitute. The aim of this investigation was to study the effect of surface topography produced by the presence of microporosity on cell response, evaluating: cell attachment, cell morphology, cell proliferation, total protein content, and alkaline phosphatase (ALP) activity. HA discs with different percentages of microporosity (< 5%, 15%, and 30%) were confected by means of the combination of uniaxial powder pressing and different sintering conditions. ROS17/2.8 cells were cultured on HA discs. For the evaluation of attachment, cells were cultured for two hours. Cell morphology was evaluated after seven days. After seven and fourteen days, cell proliferation, total protein content, and ALP activity were measured. Data were compared by means of ANOVA and Duncan’s multiple range test, when appropriate. Cell attachment (p = 0.11) and total protein content (p = 0.31) were not affected by surface topography. Proliferation after 7 and 14 days (p = 0.0007 and p = 0.003, respectively), and ALP activity (p = 0.0007) were both significantly decreased by the most irregular surface (HA30). These results suggest that initial cell events were not affected by surface topography, while surfaces with more regular topography, as those present in HA with 15% or less of microporosity, favored intermediary and final events such as cell proliferation and ALP activity.

Development and regression of exercise-induced cardiac hypertrophy in rats

1979 ◽  
Vol 236 (2) ◽  
pp. H268-H272 ◽  
Author(s):  
R. C. Hickson ◽  
G. T. Hammons ◽  
J. O. Holoszy
Keyword(s):  
Cytochrome C ◽  
Protein Content ◽  
Total Protein ◽  
Female Rats ◽  
Heart Weight ◽  
Total Protein Content ◽  
Mitochondrial Marker ◽  
Time Intervals ◽  
Control Value ◽  
Exercise Induced

Adult female rats were exercised by daily swimming. All the increase in heart weight induced by the exercise occurred within 14 days and averaged 30%. The half times of the increases in heart weight and total protein content were about 4.5 days, whereas that of cytochrome c, which was used as a mitochondrial marker, was 6.5 days. The total amounts of DNA and of hydroxyproline in the heart, which were used to evaluate the degree of connective tissue hyperplasia, increased only slightly (8% and 10%, respectively). Other animals were subjected to the same swimming program for 21 days. Groups of rats were killed at various time intervals after stopping exercise. Heart weight, total protein content, and total cytochrome c content decreased rapidly initially, with 60% of the total regression of hypertrophy occurring during the first week. Thereafter, heart weight fell more gradually toward the sedentary control value. The hydroxyproline content of the heart, which was increased 10%, did not decrease during the regression of the hypertrophy.

scholarly journals Amino Acid Composition of Milk from Cow, Sheep and Goat Raised in Ailano and Valle Agricola, Two Localities of ‘Alto Casertano’ (Campania Region)

Foods ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2431
Author(s):  
Nicola Landi ◽  
Sara Ragucci ◽  
Antimo Di Maro
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Glutamic Acid ◽  
Protein Content ◽  
Total Protein ◽  
Free Amino ◽  
Raw Milk ◽  
Total Amino Acid ◽  
Total Protein Content ◽  
Campania Region

Cow, sheep and goat raw milk raised in Ailano and Valle Agricola territories (‘Alto Casertano’, Italy) were characterized (raw proteins, free and total amino acids content) to assess milk quality. Raw milk with the highest total protein content is sheep milk followed by goat and cow milk from both localities. Total amino acid content in cow, goat and sheep raw milk is 4.58, 4.81 and 6.62 g per 100 g, respectively, in which the most abundant amino acid is glutamic acid (~20.36 g per 100 g of proteins). Vice versa, the free amino acids content characteristic profiles are different for each species. In particular, the most abundant free amino acid in cow, sheep and goat raw milk is glutamic acid (9.07 mg per 100 g), tyrosine (4.72 mg per 100 g) and glycine (4.54 mg per 100 g), respectively. In addition, goat raw milk is a source of taurine (14.92 mg per 100 g), retrieved in low amount in cow (1.38 mg per 100 g) and sheep (2.10 mg per 100 g) raw milk. Overall, raw milk from ‘Alto Casertano’ show a high total protein content and are a good source of essential amino acids.

STUDYING THE INFLUENCE OF SEASONAL FACTORS ON CASEIN AND WHEY PROTEIN CONTENT IN RAW MILK AND CHEESE YIELD

2020 ◽  
pp. 142-151
Author(s):  
L. Bahdanava ◽  
A. Podryabinkina ◽  
I. Bahdanau ◽  
T. Savelyeva
Keyword(s):  
Protein Content ◽  
Whey Protein ◽  
Total Protein ◽  
Seasonal Changes ◽  
Whey Proteins ◽  
Raw Milk ◽  
National Average ◽  
Total Protein Content ◽  
Seasonal Factors

The article presents the results of research to study seasonal changes in the content of total protein, casein and whey proteins in raw milk and to analyze their impact on cheese yield. It was determined that the lowest casein content in raw milk (18% lower than the national average) was observed in October and March. The linear dependence of the cheese yield on both the total protein content and casein content was established.

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