scholarly journals The hypocholesterolaemic effects ofLactobacillus acidophilusAmerican Type Culture Collection 4356 in rats are mediated by the down-regulation of Niemann-Pick C1-Like 1

2010 ◽  
Vol 104 (6) ◽  
pp. 807-812 ◽  
Author(s):  
Ying Huang ◽  
Jinfeng Wang ◽  
Yi Cheng ◽  
Yongchen Zheng
Keyword(s):  
Lactobacillus Acidophilus ◽  
Cholesterol Metabolism ◽  
Hdl Cholesterol ◽  
Culture Collection ◽  
Liver Cholesterol ◽  
Total Serum ◽  
Total Serum Cholesterol ◽  
Down Regulation ◽  
Niemann Pick

The objective of the present study was to evaluate the effect ofLactobacillus acidophilus4356 on cholesterol metabolismin vivo.Rats were fed a cholesterol-enriched experimental diet with or withoutL. acidophilus4356 supplementation at a dose of 109 colony-forming units per d.L. acidophilus4356 feeding significantly lowered total serum cholesterol, LDL-cholesterol and TAG concentrations, but there was no change in the serum HDL-cholesterol concentrations. In addition, total liver cholesterol and TAG were decreased in theL. acidophilus4356-fed group. The expression of Niemann-Pick C1-Like 1 (NPC1L1) in the duodenum and jejunum was significantly decreased followingL. acidophilus4356 feeding.Lactobacillus acidophilus4356 increased the population of lactobacilli and bifidobacteria in the small intestine and faeces compared with the control. These results indicate that the probiotic potential of theL. acidophilus4356 strain in the control of cholesterol is at least partially mediated by the down-regulation ofNPC1L1. Furthermore, these results also potentially suggest a new mechanism that is responsible for the cholesterol-reducing effects of probiotics.

scholarly journals Identification of a novel cholesterol-lowering dipeptide, phenylalanine-proline (FP), and its down-regulation of intestinal ABCA1 in hypercholesterolemic rats and Caco-2 cells

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Arata Banno ◽  
Jilite Wang ◽  
Kenji Okada ◽  
Ryosuke Mori ◽  
Maihemuti Mijiti ◽  
...  
Keyword(s):  
Cholesterol Metabolism ◽  
Protein Hydrolysate ◽  
Hdl Cholesterol ◽  
Atherogenic Index ◽  
Down Regulation ◽  
Potential Health ◽  
Cholesterol Lowering ◽  
Active Peptide ◽  
Cholesterol Levels

AbstractThere has been no report about in vivo active cholesterol-lowering dipeptide in any protein origin, despite their potential health benefits. Cattle heart protein hydrolysate ultra-filtrate (HPHU, molecular weight < ca. 1,000 Da peptide mixture) exhibits cholesterol-lowering activity in hypercholesterolemic rats, but the active peptide in HPHU that lowers serum cholesterol levels and its molecular mechanism are unknown. In this study, we separated and purified HPHU to identify a novel cholesterol-lowering dipeptide (phenylalanine-proline, FP) and characterized the mechanism underlying its effects in vivo and in vitro. We identified FP as an active peptide from HPHU by MALDI-TOF mass spectrometry. FP significantly decreased serum total and non-HDL cholesterol and hepatic cholesterol levels in rats. FP significantly increased serum HDL cholesterol, accompanied by a significant decrease in the atherogenic index. FP also significantly increased fecal cholesterol and acidic steroid excretion. Moreover, FP significantly decreased ATP-binding cassette transporter A1 (ABCA1) expression in the rat jejunum and reduced cholesterol absorption in Caco-2 cells. We found a novel cholesterol-lowering dipeptide FP that could improve cholesterol metabolism via the down-regulation of intestinal ABCA1. The cholesterol-lowering action induced by FP was disappeared in PepT1KO mice. FP-induced cholesterol-lowering action is mediated via PepT1 in mice.

scholarly journals Effect of interventions with ingestion of legumes and/or supervised exercise on the lipid profile of young, healthy sedentary women

2010 ◽  
pp. 224-234 ◽  
Author(s):  
Luis F. Fajardo ◽  
Dora G. Castellanos ◽  
Myriam Chinchilla ◽  
Luz N. Vargas ◽  
Martha Guerra ◽  
...  
Keyword(s):  
Lipid Profile ◽  
Life Style ◽  
Ldl Cholesterol ◽  
Hdl Cholesterol ◽  
Serum Levels ◽  
Total Serum ◽  
Total Serum Cholesterol ◽  
Supervised Exercise ◽  
Healthy Life Style ◽  
Sedentary Women

Objective: To contribute to the knowledge of some aspects of the Healthy Life Style by studying the effects of including legumes in the diet and exercise at two intensity levels, along with the lipid profile of young sedentary women living at 2640 meters above sea level. Materials and methods: The study included a non-randomized clinical trial with four intervention groups: exercise at 45% VO2 peak plus legumes in diet, exercise at 65% VO2 peak plus legumes in diet, only exercise at 65% VO2 peak, and only inclusion of legumes in diet. In each group, 20 to 23 sedentary women were included. The intervention was carried out for four weeks, three days a week. Exercise prescription was based on measurement of VO2 peak by ergospirometry; and the current intervention was monitored with heart-rate monitors. The outcome variables were total serum cholesterol, LDL cholesterol, HDL cholesterol, and triacylglycerols measured at baseline and after two and four weeks of intervention. Results: The measurements taken of participants in the group of exercise at 65% VO2 peak plus legumes in diet showed a reduction of 19.8 mg/dl in total cholesterol, of 21.8 mg/dl of LDL cholesterol, of 20.7 mg/dl of tracylglycerol, and an increase of 6.2 mg/dl of HDL cholesterol. The serum levels of HDL also increased in the group of only exercise at 65% VO2 peak. No significant changes in serum levels were documented for participants in the group with only dietary modifications. Conclusions: The results suggest that different interventions that meet some of the criteria for healthy eating and life style show different effects with regards to the level of change in the lipid profile components.

THE EFFECT OF DIETARY OILS AND FATTY ACIDS ON CHOLESTEROL METABOLISM IN THE RAT

1958 ◽  
Vol 36 (1) ◽  
pp. 433-438 ◽  
Author(s):  
J. D. Wood ◽  
B. B. Migicovsky
Keyword(s):  
Fatty Acids ◽  
Total Cholesterol ◽  
Lauric Acid ◽  
Opposite Effect ◽  
Cholesterol Metabolism ◽  
Coconut Oil ◽  
Liver Cholesterol ◽  
Dietary Oils ◽  
Liver Homogenates

Rats were fed diets containing 20% oils and 9% fatty acids and the effect of these compounds on cholesterol metabolism was studied. Unsaturated oils and fatty acids increased total cholesterol in the liver and stimulated the incorporation of C14-acetate into cholesterol both in vivo and in liver homogenates. Saturated material such as coconut oil and lauric acid had the opposite effect with respect to amount of liver cholesterol and to in vivo incorporation. The saturated material had no significant effect on synthesis in homogenates. The effect of oils in the diet was rapid, the stimulating effect of rapeseed oils being observed after the rats had been placed on the diet for as short a period as 3 days.

scholarly journals Effect of feeding diets of varying fatty acid composition on apolipoprotein expression in newborn swine

1998 ◽  
Vol 275 (4) ◽  
pp. G645-G651 ◽  
Author(s):  
Heng Wang ◽  
Felicia Hunter ◽  
Dennis D. Black
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Blot Hybridization ◽  
Hdl Cholesterol ◽  
Mrna Abundance ◽  
Total Serum ◽  
Total Serum Cholesterol ◽  
Apo B ◽  
Hepatic Expression

The purpose of this study was to determine the effect of chronic (1 wk) feeding of dietary triacylglycerol (TG) of varying fatty acid composition on small intestinal and hepatic apolipoprotein expression, as well as serum lipid and apolipoprotein concentrations, in newborn swine. Two-day-old female swine were fed one of three diets by gavage with the following lipid composition: medium-chain TG (MCT; MCT oil), intermediate-chain saturated TG (ICST; coconut oil), and long-chain polyunsaturated TG (LCPUT; safflower oil) at 753 kJ ⋅ kg−1 ⋅ day−1with 51% of energy from fat. After 1 wk, serum lipids and apolipoprotein concentrations were measured, and jejunal apolipoprotein B (apo B) and apo A-I mass and apo B, apo A-I, apo A-IV, and apo C-III synthesis were measured. Liver was processed for determination of apo B and apo A-I mass and apo B, apo A-I, apo C-III, and β-actin mRNA abundance by slot blot hybridization. Compared with the MCT and LCPUT groups, the ICST group had higher total serum cholesterol, TG, high-density lipoprotein (HDL)-cholesterol, and apo A-I concentrations. There were no differences among the three groups for intestinal apolipoprotein mass or synthesis. In liver, apo A-I mass was highest in the ICST group. Liver apo A-I and apo C-III mRNA abundance was highest in the ICST group. Among all three groups, hepatic apo A-I mass correlated significantly with plasma HDL-cholesterol concentrations, and serum TG concentrations correlated with hepatic apo C-III mRNA abundance. In conclusion, we found that in the newborn piglet, chronic feeding of ICST increases serum total cholesterol, TG, HDL-cholesterol, and apo A-I concentrations and hepatic expression of apo A-I and apo C-III mRNA, compared with feeding of MCT or LCPUT. We speculate that increased hepatic apo A-I expression may contribute to the higher serum HDL and apo A-I concentrations in the ICST animals. Increased hepatic expression of apo C-III with ICST feeding may contribute to the higher serum TG concentrations by apo C-III-mediated inhibition of the catabolism of triacylglycerol-rich lipoproteins.

Trends in blood pressure, lipids, lipoproteins and glucose metabolism in the Northern Sweden MONICA project 1986 - 99

2003 ◽  
Vol 31 (61_suppl) ◽  
pp. 43-50 ◽  
Author(s):  
Jan-HÅkan Jansson ◽  
Kurt Boman ◽  
Torbjörn Messner
Keyword(s):  
Blood Pressure ◽  
Antihypertensive Drugs ◽  
Adult Population ◽  
Hdl Cholesterol ◽  
Total Serum ◽  
Total Serum Cholesterol ◽  
Northern Sweden ◽  
Population Total ◽  
The Past ◽  
Monica Project

Aim: The authors present an analysis of time trends in blood pressure, hypertension, and lipids in the adult population of Northern Sweden over the period 1986 - 99. Method: Four population surveys were undertaken with new and independent cohorts, 25 - 64 years old. Results: Small changes in blood pressure were observed during the study period with increased systolic blood pressure in men and decreased diastolic blood pressure in women. The proportion with high blood pressure was found to be constant over time and only 50% were prescribed antihypertensive drugs and of these only a minority were normotensive. Total serum cholesterol decreased from 6.4 to 5.7 mmol/l in men and from 6.3 to 5.7 mmol/l in women over the study period, and this was accompanied by an increase in HDL cholesterol by about 10% in both sexes. The proportion with high cholesterol decreased from 41% to 26%. Triglycerides increased between 1986 and 1990 and no further changes were found in the following surveys. Conclusion: In the Northern Sweden MONICA population total cholesterol has declined and HDL cholesterol has increased during the past 13 years and small changes in blood pressure have been observed for both men and women.

THE EFFECT OF DIETARY OILS AND FATTY ACIDS ON CHOLESTEROL METABOLISM IN THE RAT

1958 ◽  
Vol 36 (4) ◽  
pp. 433-438 ◽  
Author(s):  
J. D. Wood ◽  
B. B. Migicovsky
Keyword(s):  
Fatty Acids ◽  
Total Cholesterol ◽  
Lauric Acid ◽  
Opposite Effect ◽  
Cholesterol Metabolism ◽  
Coconut Oil ◽  
Liver Cholesterol ◽  
Dietary Oils ◽  
Liver Homogenates

Rats were fed diets containing 20% oils and 9% fatty acids and the effect of these compounds on cholesterol metabolism was studied. Unsaturated oils and fatty acids increased total cholesterol in the liver and stimulated the incorporation of C14-acetate into cholesterol both in vivo and in liver homogenates. Saturated material such as coconut oil and lauric acid had the opposite effect with respect to amount of liver cholesterol and to in vivo incorporation. The saturated material had no significant effect on synthesis in homogenates. The effect of oils in the diet was rapid, the stimulating effect of rapeseed oils being observed after the rats had been placed on the diet for as short a period as 3 days.

The recalcification plasma clotting time. A valuable general clotting test in man and rats

1969 ◽  
Vol 47 (8) ◽  
pp. 689-693 ◽  
Author(s):  
Serge Renaud
Keyword(s):  
Platelet Count ◽  
Plastic Material ◽  
Total Serum ◽  
Total Serum Cholesterol ◽  
Improved Method ◽  
Clotting Time ◽  
Vein Thrombosis ◽  
Hepatic Vein Thrombosis ◽  
Periodic Fluctuations

An improved method for determining the recalcification plasma clotting time (PCT) in siliconized and plastic material is described. Provided the plasma is stored at 29–30 °C, this general clotting test appears to give highly accurate and reproducible results. The PCT values were found to be related to the in vivo platelet count in rat, and to the total serum cholesterol and plasma fibrinogen in man. Serial determinations in two women indicated that the PCT was subject to periodic fluctuations connected with the menstrual cycle. Finally, it was observed that the clotting time of both platelet-rich and -poor plasma was inversely related to the severity of large hepatic vein thrombosis initiated by the injection of a S. typhosa endotoxin in hyperlipemic rats.

scholarly journals The role of cytochrome P-450 in cholesterol biogenesis and catabolism

1972 ◽  
Vol 128 (2) ◽  
pp. 237-242 ◽  
Author(s):  
Sandra D. Atkin ◽  
Eileen D. Palmer ◽  
P. D. English ◽  
B. Morgan ◽  
M. A. Cawthorne ◽  
...  
Keyword(s):  
Cholesterol Metabolism ◽  
Carbon Disulphide ◽  
Normal Serum ◽  
Liver Cholesterol ◽  
Drug Metabolizing Enzyme ◽  
Metabolizing Enzyme ◽  
Cytochrome P 450

1. Adjuvant-induced arthritis in rats is accompanied by a loss of activity of the drug-metabolizing enzyme system and a decrease in hepatic cytochrome P-450. 2. Arthritic rats have normal serum and liver cholesterol concentrations. 3. The rate of biogenesis of cholesterol in vivo and in vitro from either [14C]acetate or [14C]mevalonate in arthritic rats was the same as or greater than that found in control rats. 4. Treatment of rats with carbon disulphide (1ml/kg) resulted in a loss of drug-metabolizing-enzyme activity and increased cholesterol biogenesis. 5. The activity of cholesterol 7α-hydroxylase in adjuvant-induced arthritic rats did not differ significantly from that in control rats. 6. Rats fed with cholestyramine had an elevated hepatic cholesterol 7α-hydroxylase activity, but neither the concentration of cytochrome P-450 nor the activity of the drug-hydroxylating enzyme, aminopyrine demethylase, was affected. 7. The relationships between drug hydroxylation and cholesterol metabolism are discussed.

scholarly journals Alterations in mood after changing to a low-fat diet

1998 ◽  
Vol 79 (1) ◽  
pp. 23-30 ◽  
Author(s):  
Anita S. Wells ◽  
Nicholas W. Read ◽  
Jonathan D. E. Laugharne ◽  
N. S. Ahluwalia
Keyword(s):  
Dietary Fat ◽  
Plasma Cholesterol ◽  
Intervention Group ◽  
Hdl Cholesterol ◽  
Diet Group ◽  
Total Serum ◽  
Control Group ◽  
Total Serum Cholesterol ◽  
Low Fat ◽  
Low Fat Diet

The effects on mood of reducing dietary fat while keeping the energy constant were examined in ten male and ten female healthy volunteers aged between 20 and 37 years. Each volunteer consumed a diet containing 41% energy as fat for 1 month. For the second month half of the subjects changed to a low-fat diet (25% energy from fat) and the remainder continued to eat the diet containing 41% energy from fat. Changes in mood and blood lipid concentrations were assessed before, during and at the end of the study. Profile of mood states (POMS) ratings of anger–hostility significantly increased in the intervention group after 1 month on the low-fat diet, while during the same period there was a slight decline in anger–hostility in the control subjects (group F 6.72; df 1,14; P = 0.021). Tension–anxiety ratings declined in the control group consuming the higher fat diet but did not change in the group consuming the low-fat diet (group F 6.34; df 1,14; P = 0.025). There was a decline in fasting concentrations of HDL-cholesterol after the low-fat diet and a small increase in subjects consuming the medium-fat diet (group F 4.96; df 1,12; P = 0.046), but no significant changes in concentrations of total serum cholesterol, LDL-cholesterol or triacylglycerol were observed. The results suggest that a change in dietary fat content from 41 to 25% energy may have adverse effects on mood. The alterations in mood appear to be unrelated to changes in fasting plasma cholesterol concentrations.

scholarly journals A new antidiabetic compound attenuates inflammation and insulin resistance in Zucker diabetic fatty rats

2010 ◽  
Vol 298 (5) ◽  
pp. E1036-E1048 ◽  
Author(s):  
Min Lu ◽  
David Patsouris ◽  
Pingping Li ◽  
Jaime Flores-Riveros ◽  
James M. Frincke ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Adipose Tissue ◽  
Insulin Sensitivity ◽  
Hepatic Glucose Production ◽  
Liver Cholesterol ◽  
Total Serum ◽  
Total Serum Cholesterol ◽  
Zucker Diabetic Fatty Rats ◽  
Anti Inflammatory

Tissue macrophage inflammatory pathways contribute to obesity-associated insulin resistance. Here, we have examined the efficacy and mechanisms of action of a novel anti-inflammatory compound (HE3286) in vitro and in vivo. In primary murine macrophages, HE3286 attenuates LPS- and TNFα-stimulated inflammation. In Zucker diabetic fatty rats, inflammatory cytokine/chemokine expression was downregulated in liver and adipose tissue by HE3286 treatment, as was macrophage infiltration into adipose tissue. In line with reduced inflammation, HE3286 treatment normalized fasting and fed glucose levels, improved glucose tolerance, and enhanced skeletal muscle and liver insulin sensitivity, as assessed by hyperinsulinemic euglycemic clamp studies. In phase 2 clinical trials, HE3286 treatment led to an enhancement in insulin sensitivity in humans. Gluconeogenic capacity was also reduced by HE3286 treatment, as evidenced by a reduced glycemic response during pyruvate tolerance tests and decreased basal hepatic glucose production (HGP) rates. Since serum levels of gluconeogenic substrates were decreased by HE3286, it indicates that the reduction of both intrinsic gluconeogenic capacity and substrate availability contributes to the decrease in HGP. Lipidomic analysis revealed that HE3286 treatment reduced liver cholesterol and triglyceride content, leading to a feedback elevation of LDL receptor and HMG-CoA reductase expression. Accordingly, HE3286 treatment markedly decreased total serum cholesterol. In conclusion, HE3286 is a novel anti-inflammatory compound, which displays both glucose-lowering and cholesterol-lowering effects.

Sign in / Sign up

Export Citation Format

Share Document