DO SALICYLIC ACID, NITRIC OXIDE AND FEEDING BY MAHANARVA SPECTABILIS NYMPHS INDUCE A RESISTANCE RESPONSE IN ELEPHANT GRASS?

2013 ◽  
Vol 50 (4) ◽  
pp. 498-504 ◽  
Author(s):  
M. V. LEITE ◽  
A. M. AUAD ◽  
T. T. RESENDE ◽  
M. P. FRIAS ◽  
M. G. FONSECA ◽  
...  

SUMMARYThe objective of this study was to determine whether salicylic acid, nitric oxide (NO) and/or feeding by nymphs of the spittlebug Mahanarva spectabilis affect the concentration of total phenolic compounds and the dry matter content of different genotypes of elephant grass (Pennisetum purpureum). Four genotypes of elephant grass with varying levels of resistance to insect attack were treated with 4 mL of salicylic acid (1%) or 2 mL of NO per plant in the presence and the absence of nymphs. We determined the concentration of total phenolic compounds and the percentage dry matter of shoots and roots in the plants. We found that salicylic acid, NO and attack by M. spectabilis did not change significantly the total phenolic compounds concentration in any of the genotypes of elephant grass tested. However, we observed variations in the concentration of phenolic compounds produced between the resistant and susceptible genotypes, both in the shoot, when subjected to salicylic acid or nymphs, and in the roots in the presence of nymphs. Furthermore, the inducers resulted in variation in the dry matter content of the shoots and roots for most of the genotypes tested. Therefore, we conclude that chemical inducers and feeding by M. spectabilis nymphs cannot be used to elicit a resistance response in elephant grass by stimulating the production of phenolic compounds.

2021 ◽  
Vol 39 (3) ◽  
pp. 299-304
Author(s):  
Lucimeire Pilon ◽  
Jaqueline S Guedes ◽  
Bruna S Bitencourt ◽  
Raphael Augusto de C Melo ◽  
Larissa PC Vendrame ◽  
...  

ABSTRACT Sweetpotato (Ipomoea batatas) is a root crop grown in many countries. This tuberous root is a source of energy, nutrients, and phytochemicals. In this study, bioactive compounds and physical and physicochemical qualities of sweetpotato genotypes were evaluated. Eight new genotypes of sweetpotato produced by Embrapa Hortaliças (orange-fleshed: MD09026-OF and MD09024-OF; cream-fleshed: MD09011-CF, MD09004-CF, MD10039-CF, and MD10004-CF; yellow-fleshed: MD09017-YF and MD12002-YF) and two cultivars used as controls (Beauregard and Brazlândia Roxa) were evaluated for color, soluble solids, dry matter, phenolic compounds, total carotenoids and β-carotene. Hue angles differed even between those sweetpotatoes with the same flesh color. The orange-fleshed genotypes MD09024-OF, MD09026-OF, and Beauregard, had the lowest L*, showing to be darker than the others. These sweetpotatoes also had the brightest flesh colors with higher C*. The orange-fleshed genotypes MD09026-OF and MD09024-OF were sweeter (10.55oBrix and 9.23oBrix) than Beauregard (5.12oBrix). Brazlândia Roxa had the highest dry matter content (38.05%), followed by the genotypes MD10004-CF, MD09017-YF, MD09026-OF MD10039-CF, and MD09011-CF, which showed similarity, ranging from 32.33% to 29.12%. The highest contents of total carotenoids were found for the orange-fleshed genotypes MD09026-OF (80.06 mg g-1) and MD09024-OF (70.56 mg g-1) and Beauregard (73.12 mg g-1). These same genotypes showed the highest total phenolic compounds (0.815 mg g-1 and 0.686 mg g-1, respectively). MD09026-OF showed the highest content of β-carotene (46.47 mg g-1). MD09026-OF was the most prominent genotype among those evaluated, as it showed the highest total carotenoid, β-carotene, phenolic compounds, and soluble solids content, in addition to a high dry matter content.


2017 ◽  
pp. 3-12
Author(s):  
Audrey Pissard ◽  
Vincent Baeten ◽  
Pierre Dardenne ◽  
Pascal Dupont ◽  
Marc Lateur

Description of the subject. The article deals with the use of near-infrared spectroscopy (NIR) on fresh apples to determine the phenolic compounds and dry matter content in peel and flesh powders. Objectives. The aim is the rapid and non-destructive determination of these nutritional parameters. Method. Two hundred twenty-nine fruits from 20 varieties were analyzed with NIR spectroscopy and reference methods. Results. Great variability of total phenolic compounds (TPC) in peel and flesh powders was observed among varieties. The dry matter (DM) content also differed greatly between peel and flesh. Calibration and validation models showed high coefficients of determination for the TPC content, which were slightly higher for the peel than for the flesh (R² val = 0.91 and 0.84 respectively). For the DM content, high coefficients of determination and ratios of prediction to deviation (RPDs) were also observed (R² val = 0.94, RPD = 4.8 and R² val = 0.94, RPD = 4.9 for the peel and flesh respectively). Conclusions. Calibration and validation models allow quantitative predictions to be made for TPC and DM content. They confirm the potential of NIRS for predicting the polyphenol content and highlight its potential for determining the DM content, a parameter often neglected in research into apple quality.


Molecules ◽  
2020 ◽  
Vol 25 (21) ◽  
pp. 4884
Author(s):  
Khadijeh Yasaminshirazi ◽  
Jens Hartung ◽  
Michael Fleck ◽  
Simone Graeff-Hoenninger

The growing interest of consumers in healthy organic products has increased the attention to the organic production of beetroot. In this regard, six field experiments were conducted in 2017 and 2018 in three different locations under the specific conditions of organic agriculture, and fifteen beetroot genotypes, including one F1 hybrid as a commercial control and one breeding line, were compared regarding the content of the total dry matter, total soluble sugar, nitrate, betalain, and total phenolic compounds in order to investigate the genetic potential of new and existing open-pollinated genotypes of beetroot regarding the content of their bioactive compounds. The results of this study indicated a significant impact of genotype (p < 0.05) on all measured compounds. Furthermore, results revealed a significant influence of the interactions of location × year (p < 0.05) on the beetroot composition, and, thus, the role of environmental conditions for the formation of tested compounds. The total dry matter content (TDMC) of beetroots varied between 14.12% and 17.50%. The genotype ‘Nochowski’, which possessed the highest total soluble sugar content with 14.67 °Bx (Brix), was among the genotypes with the lowest nitrate content. On the contrary, the cylindrical-shaped genotype ‘Carillon RZ’ (Rijk Zwaan), indicated the lowest sugar content and the highest nitrate concentration. The amount of total phenolic compounds ranged between 352.46 ± 28.24 mg GAE 100 g−1 DW (milligrams of gallic acid equivalents per 100 g of dry weight) and 489.06 ± 28.24 mg GAE 100 g−1 DW for the red-colored genotypes which is correlated with the high antioxidant capacity of the investigated genotypes. Due to the specifics of the required content of bioactive compounds for various products, the selection of suitable genotypes should be aligned with the intended final utilization.


2019 ◽  
Vol 40 (5) ◽  
pp. 2045 ◽  
Author(s):  
Flávio Pinto Monção ◽  
Marco Aurélio Moraes Soares Costa ◽  
João Paulo Sampaio Rigueria ◽  
Marielly Maria Almeida Moura ◽  
Vicente Ribeiro Rocha Júnior ◽  
...  

The objective was to evaluate the productivity, chemical composition, ruminal degradability of dry matter and digestibility of BRS capiaçu grass (Pennisetum purpureum Schum.) managed at five regrowth ages. A completely randomized design with ten replications was used in the BRS capiaçu elephant grass subjected to five cutting intervals (30, 60, 90, 120 and 150 days) in the summer, making a total of 50 plots with a useful area of 4 x 2 m. There were daily increases in the dry matter production in the order of 382 kg ha-1, with 49,859 kg ha-1 being produced at 150 regrowth days. The dry matter content and organic matter linearly increased (P < 0.01) at different regrowth ages. The crude protein content, in vitro dry matter digestibility and in vitro digestibility of neutral detergent fiber linearly reduced (P < 0.01) 0.037%, 0.196% and 0.256% per day, respectively. Potential degradability of dry matter decreased from 68.9% at 30 days to 44.7% at 150 regrowth days (0.194 percentage units per day). The rate of degradation of fraction B 'c' was not modified (P = 0.94), averaging 1.46% hour-1. In the cultivation of BRS capiaçu elephant grass in the summer season, in the northern region of Minas Gerais, the age for harvesting between 90 and 120 days of regrowth is recommended.


Foods ◽  
2019 ◽  
Vol 8 (8) ◽  
pp. 307 ◽  
Author(s):  
Pratap Madane ◽  
Arun Das ◽  
Mirian Pateiro ◽  
Pramod Nanda ◽  
Samiran Bandyopadhyay ◽  
...  

The present work investigated the efficacy of Moringa flower (MF) extract to develop a functional chicken product. Three groups of cooked chicken nuggets—control (C), T1 (with 1% MF) and T2 (2% MF)—were elaborated and their physicochemical, nutritional, storage stability and sensory attributes were assessed during refrigerated storage at 4 °C up to 20 days. In addition, MF extracts were characterised in terms of chemical composition, total phenolic content and its components using high-performance liquid chromatography with a diode-array detector (HPLC-DAD), dietary fibre and antioxidant capacity. MF contained high protein (17.87 ± 0.28 dry matter), dietary fibre (36.14 ± 0.77 dry matter) and total phenolics (18.34 ± 1.16 to 19.49 ± 1.35 mg gallic acid equivalent (GAE)/g dry matter) content. The treated nuggets (T1 and T2) had significantly enhanced cooking yield, emulsion stability, ash, protein, total phenolics and dietary fibre compared to control. Incorporation of MF extract at 2% not only significantly reduced the redness/increased the lightness, but also decreased the hardness, gumminess and chewiness of the product compared to control. Moreover, the addition of MF extract significantly improved the oxidative stability and odour scores by reducing lipid oxidation during storage time. Sensory attributes of nuggets were not affected by the addition of MF extract and the products remained stable and acceptable even on 15th day of storage. These results showed that MF extract could be considered as an effective natural functional ingredient for quality improvement and reducing lipid oxidation in cooked chicken nuggets.


2021 ◽  
Vol 28 (2) ◽  
Author(s):  
Jaunė Blažytė ◽  
Nijolė Vaitkevičienė

The aim of this work was to investigate and compare the amounts of biologically active compounds, dry matter and soluble solids in pulp and peels of four pear cultivars (‘Ksena’, ‘Beloruskaja pozdniaja’, ‘Alna’ and ‘Aleksandr Lucas’). The research was carried out at Vytautas Magnus University Agriculture Academy (Lithuania) in 2020. Four pear cultivars (‘Ksena’, ‘Beloruskaja pozdniaja’, ‘Alna’ and ‘Aleksandr Lucas’) were cultivated at a farm in the Joniškis District of Lithuania. Pears were grown following traditional pear production technology. The amounts of dry matter, soluble solids, vitamin C and fiber were determined using the standard method. The total content of phenolic compounds was determined using a Folin-Ciocalteu reagent with a UV/VIS spectrophotometer. The research results showed that significantly the highest amounts of soluble solids were found in the ‘Alna’ pear fruit pulp (14.25% f. w.). The ‘Alna’ peel sample had significantly the highest amount of total phenolic compounds (1290.99 mg 100 g–1 d. w.). The highest content of dry matter was found in the ‘Aleksandr Lucas’ pear fruit peel (24.67%). The ‘Aleksandr Lucas’ and ‘Beloruskaja pozdniaja’ pear fruit peel contained significantly the highest amounts of vitamin C (2.70 and 2.63 mg 100 g–1 f. w., respectively). The highest content of fiber was found in the ‘Ksena’ fruit peel (36.12% d. w.). The investigated pear peel sample had significantly higher amounts of dry matter, vitamin C, total phenolic compounds and fiber than the pulp. However, the pulp contains the highest contents of soluble solids.


2020 ◽  
Vol 33 (4) ◽  
pp. 264-272
Author(s):  
Cláudia M Serra-Ferreira ◽  
Agatha G Farias-Souza ◽  
Rita C Almeida-Mendonça ◽  
Melany Simões-Souza ◽  
Wagner R L Lopes-Filho ◽  
...  

Background: Tropical grasses, such as elephant grass, have high moisture content during its ideal phenological state for silage. High moisture content hinders proper preservation and reduces the nutritive value of silage due to secondary fermentation and production of effluents. Adding feed materials with high dry matter content, such as murumuru (Astrocaryum murumuru) meal, is a potential alternative to improve silage yield. Objective: To determine the effects of including murumuru meal (0, 7, 14, 21, and 28%) on the fermentative characteristics, microbiological activity, aerobic stability, and chemical composition of elephant grass silages. Methods: A completely randomized design with five treatments and five replicates was used. Elephant grass was collected at 60 d of age, minced, and homogenized with murumuru meal. The mass was placed in experimental 15-L silos. The silos were collected and analyzed 45 d later. Results: Effluent production decreased (p<0.05) as the proportions of murumuru meal in silage increased. A quadratic effect (p<0.05) was observed on dry matter recovery. An increase (p<0.05) was observed in dry matter content, a decrease (p<0.05) in the neutral detergent fiber content, and an increase (p<0.05) in the non-fibrous carbohydrate content with the inclusion of murumuru meal. Conclusions: Addition of murumuru meal improves chemical composition and does not affect the fermentative characteristics of elephant grass silage, while it reduces effluent losses. Nevertheless, the inclusion of murumuru meal in the elephant grass silage decreased the time of aerobic stability.


Author(s):  
Henrique Guimarães De Favare ◽  
Joadil Gonçalves De Abreu ◽  
Livia Vieira De Barros ◽  
Felipe Gomes Da Silva ◽  
Luis Miguel Mendes Ferreira ◽  
...  

Aimed to evaluate the bromatological composition of different genotypes of elephant grass (Pennisetum purpureum Schum.) to energy production through combustion. The experimental design was a randomized block with 3 repetition and the treatments arranged in a subdivided plots scheme, considering as a plots the thirteen genotypes and harvests (dry and rainy) as subplots. The genotypes evaluated were Cubano Pinda, Porto Rico, Vrukwona, Piracicaba 241, Cuba 116, Taiwan A-25, Mecker, Napier, Canará, Guaçu, Cameroon, CNPGL 93-41-1 and CNPGL 91-25-1 clones. The experiment lasted two consecutive years with harvests made every 6 months, with a harvest in the dry season (September) and another one in the rainy season (March), totaling 4 harvests. For dry matter content analysis, three tillers were selected at random and dried in an oven at 55ºC until reaching a constant mass. For biomass quality analysis, the samples were ground in Willey type mills with 1 mm sieves, submitted to bromatological analysis to determine the neutral detergent fiber, acid detergent fiber, hemicellulose, volatile materials, and fixed carbon content. Higher levels of dry matter (greater than 44.4%), acid detergent fiber (greater than 44.8%), volatile matter (greater than 94.3%) and higher calorific value (greater than 3,450 kcal kg-1) occur in the dry period of the year and in genotypes Mercker, Piracicaba 241, Guaçu and BRS Canará genotypes.


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Manuwat Tintarasara Na Ratchaseema ◽  
Lawan Kladsuwan ◽  
Laurent Soulard ◽  
Pratchya Swangmaneecharern ◽  
Prapat Punpee ◽  
...  

AbstractThe objective of this research was to study the effect of Benzothiadiazole (BTH) and Salicylic acid (SA) on the systemic acquired resistance (SAR) of sugarcane the phytoplasma associated with the sugarcane white leaf (SCWL) disease. The experiment was conducted on plants of the sugarcane variety Khon Kaen 3 (KK3) infected with SCWL phytoplasma using insect vectors. Biochemical changes related to the SAR such as SA and total phenolic compounds were followed according to 4 different timepoints: 7, 14, 21 and 28 days after inoculation. Together, phytoplasma were quantified by RT-qPCR using the secA gene of phytoplasma. According to our results, the spraying of BTH and SA tended to increase the amounts of SA, total phenolic compounds and a lower presence of phytoplasma in the plants in comparison with the inoculated control. Spraying BTH at a concentration of 2.4 mM and SA at a concentration of 2.4 mM exhibited the best efficiency to reduce the concentration of phytoplasma. According to RT-qPCR results, the inoculated plants sprayed with BTH displayed a significantly lower concentration of phytoplasma compared to the inoculated controls. Overall, our results indicated that the spray of BTH and SA could induce an efficient SAR response to the phytoplasma associated with the SCWL disease. We expect these results will give support to the development of new products for controlling white leaf disease in sugarcane.


2021 ◽  
Vol 8 (1) ◽  
pp. 19
Author(s):  
Joel B. Johnson ◽  
Janice S. Mani ◽  
Mani Naiker

Habanero chillies (Capsicum chinense cv Habanero) are a popular species of hot chilli in Australia, with their production steadily increasing. However, there is limited research on this crop due to its relatively low levels of production at present. Rapid methods of assessing fruit quality could be greatly beneficial both for quality assurance purposes and for use in breeding programs or experimental growing trials. Consequently, this work investigated the use of infrared spectroscopy for predicting dry matter content, total phenolic content and capsaicin/dihydrocapsaicin content in 20 Australian Habanero chilli samples. Near-infrared spectra (908–1676 nm) taken from the fresh fruit showed strong potential for the estimation of dry matter content, with an R2cv of 0.65 and standard error of cross-validation (SECV) of 0.50%. A moving-window partial least squares regression model was applied to optimise the spectral window used for dry matter content prediction, with the best-performing window being between 1224 and 1422 nm. However, the near-infrared spectra could not be used to estimate the total phenolic content or capsaicin/dihydrocapsaicin content of the samples. Mid-infrared spectra (4000–400 cm−1) collected from the dried, powdered material showed slightly more promise for the prediction of total phenolics and the ratio of capsaicin-to-dihydrocapsaicin, with an R2cv of 0.45 and SECV of 0.32 for the latter. The results suggest that infrared spectroscopy may be able to determine dry matter content in Habanero chilli with acceptable accuracy, but not the capsaicinoid or total phenolic content.


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