Chitosan and microbial inoculants in whole-plant soybean silage

2021 ◽  
pp. 1-9
Author(s):  
J. P. G. de Morais ◽  
R. Cantoia Júnior ◽  
T. M. Garcia ◽  
E. Capucho ◽  
M. Campana ◽  
...  
Keyword(s):  
Block Design ◽  
Microbial Inoculants ◽  
Fresh Matter ◽  
Randomized Block Design ◽  
Whole Plant ◽  
Ruminant Diets ◽  
Fermentation Profile ◽  
Soybean Plants ◽  
Branched Chain

Abstract Whole-plant soybean silage (WPSS) is a potential high-protein roughage source for ruminant diets. However, WPSS can be difficult to ensile and fermentation is a challenge. This study was conducted to evaluate the effect of chitosan and microbial inoculants on fermentation profile, fermentation losses, chemical composition, and in vitro degradation of WPSS. Forty experimental silos (PVC tubing with 28 cm i.d. and 25 cm height) were produced. Soybean plants from 10 plots were ensiled in a completely randomized block design to evaluate the following treatments: (1) control (CON): WPSS without additives; (2) chitosan (CHI): WPSS additive with 6 g/kg DM of chitosan; (3) LBB: WPSS treated with 5.0 × 107 colony-forming units (CFU) of Lactobacillus buchneri (NCIM 40788) per kg of fresh matter and (4) LPP: WPSS treated with 1.6 × 108 CFU of Lactobacillus plantarum and 1.6 × 108 CFU of Pediococcus acidilactici per kg of fresh matter. Silos were opened 120 days after ensiling. Microbial inoculants reduced silage pH, whereas LPP-treated silos showed the lowest concentration of NH3-N, ethanol, butyric, acetic, branched-chain, and propionic organic acids. LBB-treatment decreased lactic acid bacteria (LAB) count relative to other treatments, and LPP-treatment showed the lowest fermentation losses, improving dry matter (DM) recovery. Relative to other treatments, LPP increased silage DM, organic matter, and decreased acid detergent insoluble crude protein (CP), improving DM and neutral detergent fibre in vitro degradation. Treatments showed no effect on silage aerobic stability. Thus, LPP-treatment improves fermentation profile, reduces fermentation losses, and increases the nutritional value of WPSS.

scholarly journals Nutritional value of sorghum silages

2017 ◽  
Vol 39 (2) ◽  
pp. 137
Author(s):  
Marielly Maria Almeida Moura ◽  
Daniel Ananias de Assis Pires ◽  
Renê Ferreira Costa ◽  
Daniella Cangussú Tolentino ◽  
João Paulo Sampaio Rigueira ◽  
...  
Keyword(s):  
Crude Protein ◽  
Block Design ◽  
Significance Level ◽  
Dry Matter Digestibility ◽  
Randomized Block Design ◽  
Nutritional Characteristics ◽  
High Participation ◽  
Sorghum Genotypes

 This study aimed to evaluate the nutritional characteristics and quality of silages of five sorghum genotypes, namely: Volumax, AG2005E, Qualimax, BRS610 and AG2501. The study was conducted at the Experimental Farm Unimontes, in the municipality of Janaúba, state of Minas Gerais. The treatments were distributed in a randomized block design with four replicates. Data were tested by analysis of variance andmeans were compared by Scott-Knott test at a significance level of 5%. The pH values ranged from 3.93 and 4.10. Genotypes differed in all studied nutritional characteristics (p < 0.05). For the acid detergent fiber, differences were detected between the genotypes, AG2501 (35.66%), Volumax (34.89%), AG2005E (34.53%), Qualimax (32.39%) and BRS610 (33.60%). The high participation of lignin was verifiedin all genotypes except for Volumax (4.14%). All silages were within the recommended range 50-65% for in vitro dry matter digestibility and classified as having good quality, however only the AG2005E genotype met nitrogen requirements for microbial fermentation, with 7.06% crude protein. 

scholarly journals Diallel analysis of maize resistance to Phaeosphaeria maydis

2004 ◽  
Vol 61 (1) ◽  
pp. 36-42 ◽  
Author(s):  
Herberte Pereira da Silva ◽  
José Roberto Moro
Keyword(s):  
Disease Severity ◽  
Block Design ◽  
Genetic Resistance ◽  
Diallel Analysis ◽  
Inbred Lines ◽  
Randomized Block Design ◽  
Whole Plant ◽  
Sources Of Variation ◽  
Specific Hybrid ◽  
Additive Genetic Effects

In breeding programs directed towards genetic resistance against diseases, the estimation of genetic parameters that control resistance allows the introduction of resistance into suscetible germplasm to be clearly focused. The purposes of this study were to estimate heterosis effects, and the general (GCA) and specific (SCA) combining abilities by using two rating methods for resistance to Phaeosphaeria maydis in a diallel analysis of 36 F1 maize hybrids and their nine inbred lines. Trials were conducted in three environments. Disease severity was evaluated in the whole plant (PI) and in the leaf positioned just below the point of insertion of the main ear (AFA). The trials followed a randomized block design with plots represented by a 5 m long rows. Differences among combining ability estimates for different environments and in both evaluation methods showed significant effects (P < 0.01) for environment (E), GCA, and GCA × E. The SCA, and SCA × E effects were not significant for any of the disease severity variables. The GCA effects were more important than SCA for this set of inbred lines, suggesting that additive genetic effects are the most important sources of variation for this trait. Heterosis effects for resistance were estimated, and it was possible to identify specific hybrid combinations between lines which have high potential for genetic control of this pathogen. Results for both disease severity variables were practically identical, even though the PI method was more convenient to use.

scholarly journals Optimation of In vitro Lateral Shoots Multiplication of Papaya (Carica papaya L.) “Callina” with BAP and NAA

2017 ◽  
Vol 4 (3) ◽  
pp. 102-107
Author(s):  
Darda Efendi ◽  
Mirza R Putra
Keyword(s):  
Carica Papaya ◽  
Clonal Propagation ◽  
Block Design ◽  
Shoot Multiplication ◽  
Plant Materials ◽  
Randomized Block Design ◽  
Lateral Shoots ◽  
Shoot Production ◽  
Set Up

Papaya is a popular fruit and is grown commercially in many subtropical and tropical countries. Papayas are generally grown from seeds; therefore the offsprings are not true-to-type and could come in three sexes, female, male, and hermaphrodite. Clonal propagation is required to obtain to grow true-to-type hermaphrodite papayas. In this research, we developed an in vitro protocol for shoot multiplication from lateral shoots from in vitro germinated papaya seedlings. The in vitro propagated plant materials could potentially be used as a source of papaya micro cuttings, or as scion for papaya grafting. The experiment was set up as a factorial experiment with NAA at 0, 0.1 and 0.5 mg.L-1, and BAP at 0, 0.1, 0.5, and 1.0 mg.L-1 in a completely randomized block design. BAP interacted with NAA in affecting the shoot production per explant. The optimum BAP and NAA concentration to produce lateral shoots was 0.54 mg. L-1 and 0.1 mg.L-1, respectively. Media without NAA reduced the number of lateral shoots and number of leaf per explant at any BAP concentration.Keywords: hermaphrodite, seeds, true to type, clonal propagation, micro cuttings.

scholarly journals Control of Conyza spp. with sequential application of glufosinate in soybean pre-sowing

2020 ◽  
Vol 50 (9) ◽  
Author(s):  
Alfredo Junior Paiola Albrecht ◽  
Leandro Paiola Albrecht ◽  
André Felipe Moreira Silva ◽  
Romulo Augusto Ramos ◽  
Natália Buttini Corrêa ◽  
...  
Keyword(s):  
Plant Height ◽  
Seed Production ◽  
Management System ◽  
Block Design ◽  
Agronomic Performance ◽  
General Control ◽  
Crop Injury ◽  
Randomized Block Design ◽  
Soybean Plants

ABSTRACT: Conyza spp. is among the main weeds reported worldwide. Due to its aggressiveness, such as high seed production and dispersion, and the growing reports of biotypes resistant to glyphosate, paraquat, and other herbicides, different control practices are required. Thus, this study aimed to evaluate the efficacy of herbicides with sequential application of glufosinate in soybean pre-sowing for control of Conyza spp. with indicative of resistant to paraquat. The study was carried out in the field, at Assis Chateaubriand and Palotina, state of Paraná, Brazil, in the 2018/19 season. The experiments were conducted in a randomized block design with four replications. The treatments consisted in application of glyphosate, 2,4-D, saflufenacil, glufosinate, saflufenacil/imazethapyr, diclosulam, paraquat/diuron, paraquat and imazethapyr/flumioxazin, at different combinations, in soybean pre-sowing. Control of Conyza spp., crop injury to soybean plants and variables related to agronomic performance (plant height and yield) were evaluated. All treatments were selective for soybean, which showed stronger crop injury in the presence of diclosulam herbicide, but this did not compromise soybean agronomic performance. In general, control levels were high for the treatments used. Except for paraquat treatments, in the area with the highest frequency of Conyza spp. with indicative of resistant to paraquat, and imazethapyr/flumioxazin treatment in both areas. These control results emphasized the importance of glufosinate in this management system and showed promising results for saflufenacil/imazethapyr.

scholarly journals Uji Efektivitas Rizobakteria Dalam Menghambat Perkembangan Penyakit Hawar Pelepah Daun (Rhizoctonia solani Kuhn.) Pada Padi Secara In Vitro

2020 ◽  
Vol 16 (1) ◽  
pp. 44
Author(s):  
Hanisa Desy Ariani ◽  
Noor Aidawati ◽  
Dewi Arika Adriani
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Rhizoctonia Solani ◽  
Plant Protection ◽  
Block Design ◽  
Sheath Blight ◽  
In Vitro Test ◽  
Sheath Blight Disease ◽  
Randomized Block Design ◽  
Blight Disease

One of the causes of the declining productivity of rice is sheath blight disease caused by the mold Rhizoctonia solani Kuhn. Control of sheath blight disease that is often done by the farmers is by using chemical pesticides (fungicides), which caused environmental problems. One way to reduce the use of pesticides is to biological control by using antagonist bacteria. This study aimed at in vitro test of rhizobacteria in preventing the development of sheath blight disease in rice plants. This research was conducted in the Phytopathology laboratory of Plant Protection Department of Faculty Agriculture, University of Lambung Mangkurat Banjarbaru from March to May 2018. The experiment used a randomized block design with three groups consisting of eight types of rhizobacteria isolates: (r1) Pseudomonas aeruginosa (Barito Kuala), (r2) Bacillus megaterium (Hulu Sungai Tengah), (r3) Azotobacter sp. (Barito Kuala), (r4) Pseudomonas sp. (Hulu Sungai Selatan), (r5) Flavobacterium sp. (Tanah Laut), (r6) Bacillus bodius (Barito Kuala), (r7) Pseudomonas aeruginosa (Hulu Sungai Selatan), (r8) Necercia sp. (Tanah Laut). The results showed that all rhizobacteria have the ability to inhibit the development of R. solani with different percentages of inhibitions. Pseudomonas aeruginosa (Barito Kuala) was the most effective rhizobacteria in inhibiting the development of R. solani.

scholarly journals Effect of Microbial Inoculants on Plant Attributes and Nutrients Uptake by Soybean in Vertisols

2021 ◽  
pp. 102-109
Author(s):  
Sanjeet Kumar ◽  
R. K. Sahu ◽  
R. K. Thakur ◽  
Bablu Yaduwanshi ◽  
N. G. Mitra
Keyword(s):  
Crop Production ◽  
Plant Biomass ◽  
Block Design ◽  
Soil Health ◽  
Microbial Consortia ◽  
Microbial Inoculants ◽  
Randomized Block Design ◽  
Nutrients Uptake ◽  
Agricultural Chemistry ◽  
Plant Attributes

The present study was carried out during kharif season 2019-20 at the Research Farm, Department of Soil Science & Agricultural Chemistry, Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur, Madhya Pradesh (INDIA), to assess the effect of microbial inoculants on plant attributes and nutrients uptake by soybean in Vertisols. The experiment was laid out under randomized block design (RBD) with three replications. The 15 treatments comprised of different beneficial microbial consortia in possible combinations applied as seed treatments. The crop was supplemented with recommended dose of fertilizers 20 N : 80 P2O5 : 20 K2O kg ha-1. Besides these, two control plots were maintained as fertilized un-inoculated control (FUI) and unfertilized un-inoculated control (UFUI). The findings revealed that the significant improvement were noticed by the application of consortia NPK+EM+PGPR in plant growth attributes of nodulation at 25, 45 & 65 DAS (71, 70 & 59% respectively), over control (9.5, 33.4 & 34.7 nodule plant-1) and its biomass, (62, 69 & 74% respectively),over the control  (0.58, 1.16 & 0.99 g plant-1),  plant height at 25, 45 & 65DAS were increased 61, 40, 41% respectively, over the control (16.20, 34.90 and 44.30 cm) and plant biomass, (48, 62 & 53%), over the control 1.67, 4.73 and 6.1 g plant-1. Similarly, nutrient uptake (seed & stover) were also increased at 25, 45 and 65 stages of crop growth, with 36.6, 34.8 & 51.3% in seed and 66.7, 98.2 & 67.2% in straw respectively over the control (98.5, 63.8, 5.2, and 7.4, 24.9 and 44.4 kg ha-1 respectively). Thus, it may be concluded that the consortium of NPK + EM + PGPR was superior for sustainable crop production and soil health.

scholarly journals APLIKASI PUPUK DAN MULSA JERAMI PADA TANAMAN KEDELAI TERHADAP SERANGAN HAMA DAN PRODUKSI

EUGENIA ◽  
2015 ◽  
Vol 21 (1) ◽  
Author(s):  
J. Rimbing ◽  
J. Pelealu ◽  
D. Sualang
Keyword(s):  
Insect Pests ◽  
Block Design ◽  
Insect Pest ◽  
Inorganic Fertilizer ◽  
Flying Insects ◽  
Randomized Block Design ◽  
Soybean Pest ◽  
Crop Damages ◽  
Virus Attack ◽  
Soybean Plants

ABSTRACT   The research was arranged in a randomized block design (RBD), with 10 treatments and 3 replications. To get the active insects pests, net sweepings were done for the flying insects, while other pests were observed directly on the plants. Collecting data of plant damages were made by systematic diagonal lines, except for viruses, census was conducted on each experimental plot.  The results of this experiment documented 12 species of insect pests attacking soybean plants. Soybean pest populations were relatively low, except for Aphis glycine that was quite high. In all treatments, plant damage caused by sucking pest Piezodorus sp and Riptortus linearis were relatively low of 1.53 % to 1.94 %; it indicated an insignificant effect to decline the production. Crop damages by virus showed a significant effect. The lowest virus attacks found on compost of 5 tons per ha with 43 plants infected per treatment, the highest virus attack showed on combination of 5 tons compost per ha + inorganic fertilizer + mulch, in which there were 274 infected plants per treatment. Apparently virus attacks gave impact on soybean production. The highest production were documented on treatment of 5 tons compost per ha with production of 0.55 tons per ha, while the lowest production was found on combination of 5 tons compost per ha + inorganic fertilizer + mulch, in which the production was 0.22 tons per ha. Keywords:  fertilizer, mulch, insect pest, production

scholarly journals The Effect of Processing and Cooling Methods on Coleus tuberosus in vitro Starch Digestibility

2021 ◽  
Vol 41 (3) ◽  
pp. 238
Author(s):  
Jhauharotul Muchlisyiyah ◽  
Tri Dewanti Widyaningsih ◽  
Retno Wulansari ◽  
Hera Sisca Prasmita
Keyword(s):  
Resistant Starch ◽  
Room Temperature ◽  
Potato Starch ◽  
Block Design ◽  
In Vitro Digestibility ◽  
Randomized Block Design ◽  
Two Factors ◽  
Cooling Methods ◽  
Total Starch

Coleus tuberosus, also known as black potato, is one of the Indonesian local tubers consumed as a carbohydrate substituent. Therefore, this study aimed to examine the effect of processing and cooling methods on the in vitro digestibility of black potato starch. Furthermore, two factors Randomized Block Design with a 2x3 experimental design was used, which consisted of processing methods (boiling, roasting, and microwave) and cooling at room temperature and 4 °C for 24 hours with 3 repetitions. Black potato flour was compared with the raw form, by assessing some parameters, namely Resistant Starch (RS), Slowly Digestible Starch (SDS), Rapidly Digestible Starch (RDS), and Glycemic Index (GI). Also, the analysis of total starch, moisture, and color was performed, hence raw black potatoes generally have 10% resistant starch (%wb). Different treatments of cooking and cooling had a significant effect (α = 0.05) on moisture content, total starch, RS, RDS, SDS, GI, brightness (L), and yellowness (b). Black potatoes subjected to the processing method followed by cooling had lower RDS and increased RS content. Furthermore, refrigeration at 4°C for 24 hours reduced the digestibility of black potato starch more than cooling at room temperature. Contrarily, microwaved black potato cooled at room temperature showed a higher digestion rate compared to the raw counterpart. Conclusively, processing followed by cooling reduces the GI and increases the RS content of Coleus tuberosus.

scholarly journals Action of auxin inhibitors on growth and grain yield of soybean

Revista CERES ◽  
2013 ◽  
Vol 60 (5) ◽  
pp. 621-628 ◽  
Author(s):  
Gederson Luiz Buzzello ◽  
Michelangelo Muzell Trezzi ◽  
José Abramo Marchese ◽  
Elouize Xavier ◽  
Edemir Miotto Junior ◽  
...  
Keyword(s):  
Grain Yield ◽  
Plant Height ◽  
Negative Correlation ◽  
Block Design ◽  
Sowing Date ◽  
Growth And Yield ◽  
Fertility Level ◽  
Randomized Block Design ◽  
Soybean Genotypes ◽  
Soybean Plants

Soybean genotypes grown in sub-tropical climate may exhibit lodging. The plant lodging is influenced by soil type and fertility level, sowing date, latitude and altitude of the location, plant population and conditions of crop development. Plant regulators and herbicides are able to avoid or reduce plant lodging. This study aimed to verify the effects of the growth regulators TIBA and daminozide on vegetative growth and yield of soybean cultivar CD 214 RR. The experiment was carried out at a field in randomized block design with four replications in a factorial scheme. The A factor was represented by the combination of regulators TIBA and daminozide and its concentrations, and the Factor B was seven times of evaluation of injury and plant height or eight times of evaluation of lodging. In the range of doses used, the application of daminozide resulted in greater injury to soybean plants than TIBA. The smaller plant height was achieved by the application of 6 g ha-1 of TIBA and 1200 g ha-¹ of daminozide. Treatments with daminozide (100 g ha-¹) and TIBA (10 g ha-1) stood out due to the reduced lodging of soybean plants. Grain weight increased linearly when the levels of TIBA increased. There was a negative correlation between lodging and grain yield and a positive correlation between plant height and lodging. There was also a negative correlation between injury caused by the application of plant regulators and lodging.

Use of agricultural waste material as an alternative substrate in cabbage seedling production and development

2020 ◽  
pp. 131
Author(s):  
Paola Alfonsa Vieira Lo Monaco ◽  
Josean de Castro Vieira ◽  
João Nacir Colombo ◽  
Marcelo Rodrigo Krause ◽  
Gustavo Haddad Souza Vieira ◽  
...  
Keyword(s):  
Agricultural Waste ◽  
Block Design ◽  
Stem Diameter ◽  
Pine Bark ◽  
Seedling Production ◽  
Coconut Fiber ◽  
Fresh Matter ◽  
Randomized Block Design ◽  
Randomized Design ◽  
Commercial Yield

Considering the need to reduce costs in the production of seedlings, the use of agricultural wastes becomes essential. The objective of this study was to evaluate a substrate composed of agricultural residues replacing the commercial substrate. The experiment was carried out in two phases. For the first phase, seedling production, we used a completely randomized design with six treatments and eight replications, using commercial substrate (Bioplant®) and five treatments with increasing proportions of moinha/descending proportions of rice husk (0/40; 10/30; 20/20; 30/10 and 40/0%), and fixed proportions of coconut fiber (15%) and pine bark (45%). We evaluated plant height, stem diameter, number of leaves, root and shoot dry masses, and Dickson quality index at 33 days after sowing. In the second experiment, cabbage development under field conditions, the treatments were maintained with four replications, using the randomized block design. We evaluated cycle length, compactness, head and stem diameter, number of basal external leaves, mass of fresh matter of the head, and commercial yield. We recommend a substrate composed of 40% moinha, 15% coconut fiber, and 45% pine bark in the production of cabbage seedlings, replacing the commercial substrate.

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