scholarly journals Dispersal of non-sporeforming anaerobic bacteria from the skin

1982 ◽  
Vol 88 (3) ◽  
pp. 487-500 ◽  
Author(s):  
Eva Benediktsdóttir ◽  
Anna Hambraeus

SummaryDispersal of non-sporeforming anaerobic bacteria was studied. Skin samples were taken from the subjects, and dispersal from different parts of the body was examined.The number of anaerobic bacteria dispersed was not correlated to their density on the surface of skin area exposed. The highest density of anaerobic bacteria on the skin was found in the face and upper trunk, but the highest yield of anaerobic bacteria dispersed came from the lower trunk.The dominant anaerobic bacteria dispersed were Propionibacterium acnes, but Propionibacterium avidum, Propionibacterium granulosum and Gram-positive cocci were also isolated from the dispersal samples. Peptococcus magnus was the most common coccus isolated. For the less frequently isolated bacteria, the best correlation was found between the perineal flora and airborne bacteria.A comparison was also made of bacterial dispersal by naked and dressed subjects. The dispersal of both aerobic and anaerobic bacteria was higher when the subjects were dressed in conventional operating theatre cotton clothing than when they were naked.The increased dispersal of anaerobic bacteria when the subjects were dressed was mainly due to increased dispersal of Propionibacterium sp.

2018 ◽  
Vol 19 (4) ◽  
Author(s):  
Anna Kędzia ◽  
Elżbieta Hołderna-Kędzia

Introduction. Juniper berries have been used in ancient in folk medicine for treatment infections, as a spice for meat, and preparation of gin and raki. Juniper (Juniperus communis L.) belongs to the Cupressaceae family. It is an evergreen tree or shrub and achieve from 2 to 10 m high. Its berries produced essential oil, which contain α-pinene, camphene, limonene, cadinene, terpinen-4-ol, myrcene, β-pinene, sabinene, flavonoids, resins, organic acids, and tannins. The oil is used for treatment various diseases. It demonstrate antimicrobial properties. Aim. The aim of the study was to investigate the susceptibility of anaerobic bacteria of oral cavity to juniper oil. Material and methods. The 56 of anaerobic bacteria isolated from patients, in it Gram-negative rods (31 strains), Gram-positive cocci (13) Gram-positive rods (12) and 8 reference strains were tested. Investigation was carried out using the plate dilution technique in Brucella agar supplemented with 5% defibrynated sheep blood, menadione and hemin. Inoculum containing 106 CFU per spot was seeded with Steers replicator upon the agar with oil or without the oil (strains growth control). Concentration of dated oil were: 2,0, 5,0, 7,5, 10,0, 15,0 and 20,0 mg/ml. The plate was incubated in anaerobic condition in anaerobic jar in 37°C for 48 hrs. The MIC was interpreted as the lowest concentration of juniper oil inhibiting the growth of tested bacteria. Results and discussion. The results of the experiments indicated, that rods from Gram-negative anaerobes, the strains belonging to the genera of Prevotella and Porphyromonas, were the most susceptible to essential oil. The MIC’s for the strains be within from 5,0 to 10,0 mg/ml. The lowest sensitive were the rods from genera Bacteroides, Parabacteroides and Tannerella (MIC > 20,0 mg/ml). From among Gram-positive cocci the most sensitive to oil were the strains from genus Finegoldia magna. The oil was active towards half of the strains in concentration < 2,5 mg/ml. The tested Gram-positive rods were the less sensitive (MIC 5,0-> 20,0 mg/ml). From this group, the strains Actinomyces viscosus demonstrated the sensitivity on 5,0 to 7,5 mg/ml. The most resistance were the rods Actinomyces odontolyticus and Propionibacterium granulosum (MIC > 20,0 mg/ml). Conclusions. The lowest susceptible to juniper oil from Gram-negative bacteria were genus of Bacteroides, Parabacteroides and Tannerella. The oil was the most active against strains of Prevotella and Porphyromonas. The cocci were the more susceptible than Gram-positive rods. The Gram-positive anaerobes showed high susceptibility to juniperus oil than Gram-negative rods.


PEDIATRICS ◽  
1981 ◽  
Vol 67 (6) ◽  
pp. 891-895
Author(s):  
Itzhak Brook ◽  
Sydney M. Finegold

Specimens from 209 cutaneous abscesses in children were cultured for aerobic and anaerobic microorganisms. Of these, nine (4%) were sterile and 51 (24%) yielded pure cultures that were predominantly Staphylococcus aureus. The rest of the abscesses yielded growth of two or more aerobic and/or anaerobic organisms. The data were organized according to these anatomic locations: head, neck, trunk, finger, nailbed, hand, leg, buttocks, perirectal, and vulvovaginal areas. Aerobic bacteria only were present in 92 specimens (46%), anaerobes only were isolated in 52 (26%), and mixed aerobic and anaerobic bacteria were present in 56 abscesses (28%). A total of 467 isolates (270 anaerobes and 197 aerobes) were recovered, accounting for 2.3 isolates per specimen (1.3 anaerobes and 1.0 aerobes). The presence of more than one anaerobe per abscess was obtained from the vulvovaginal, buttocks, perirectal, finger, nailbed, and head areas. Aerobes were more prevalent in the neck, hand, leg, and trunk areas. The predominant aerobes recovered were: S aureus (89 isolates), α- and nonhemolytic streptococci (29), group A β-hemolytic streptococci (16), Enterobacter (10), and Escherichia coli (8). The predominant anaerobes recovered were anaerobic Gram-positive cocci (79 isolates), Bacteroides sp (116, including 31 B melaninogenicus group and 29 B fragilis group), and Fusobacterium sp (39). Our findings indicate the polymicrobial nature and predominance of anaerobes in cutaneous abscesses in children in perirectal, head, finger, and nailbed areas.


1980 ◽  
Vol 94 (6) ◽  
pp. 607-615 ◽  
Author(s):  
Itzhak Brook

AbstractTympanocentesis was performed in 28 pediatric patients with chronic recurrent suppurative otitis media. The purulent aspirate was cultured aerobically and anaerobically. Aerobes only were isolated from 4(14·3 per cent) patients; five (17·9 per cent) had only anaerobes and 19 (67·8 per cent) had both aerobes and anaerobes. There were 36 aerobic isolates. P. aeruginosa was recovered in 16 patients. Other aerobes commonly recovered included Proteus sp. and S. aureus. There were 38 anaerobes isolated. Anaerobic Gram-positive cocci were isolated in 23 instances. Eleven Bacteroides sp. were recovered, including 4 B. fragilis group and 5 B. melaninogenicus. All of the patients were treated with clindamycin in a dose of 30 to 40 mg per kilogram per day divided into 3 dosages for a total period of 14 to 21 days (average 17 days). When Gram-negative enteric aerobic rods were also recovered in substantial numbers, an aminoglycoside (gentamicin) was added to the therapy (in 12 patients) and administered parenterally for 7 to 10 days. The patients' response to the therapy was excellent in 7 cases, good in 9, fair in 3 and poor in 9. There were no diarrhea episodes or colitis noted and there were no other sideeffects due to the therapy. The above findings demonstrate the polymicrobial etiology of chronic recurrent suppurative otitis media and suggest that medical treatment directed against the aerobic and anaerobic bacteria isolated from the patients' ear aspirates could eradicate the infection completely in more than half of the cases.


1986 ◽  
Vol 32 (9) ◽  
pp. 719-722 ◽  
Author(s):  
Itzhak Brook ◽  
Richard I. Walker ◽  
Thomas J. MacVittie

The presence of aerobic and anaerobic bacteria in the blood, spleen, and liver was investigated in mice that were exposed to 7, 8, 9, or 10 Gy 60Co radiation. Microorganisms were detected more often in animals exposed to higher doses of radiation. The number of mice that were culture positive and the number of isolates in one site increased with increasing dose. Bacteria were recovered in mice killed at various times after radiation, in 3 of 100 mice exposed to 7 Gy, in 13 of 100 irradiated with 8 Gy, in 23 of 90 exposed to 9 Gy, and in 34 of 87 irradiated with 10 Gy. The predominant organisms recovered were Escherichia coli, anaerobic Gram-positive cocci, Proteus mirabilis, Staphylococcus aureus, and Bacteroides spp. Escherichia coli and anaerobes were more often isolated in animals exposed to 10 Gy, while S. aureus was more often recovered in those irradiated with 9 Gy. These data demonstrate a relationship between the dose of radiation and the rate of infection due to enteric aerobic and anaerobic bacteria.


2011 ◽  
Vol 23 (12) ◽  
pp. 3001-3015 ◽  
Author(s):  
Pasha Parpia

Neuroscience folklore has it that somatotopy in human primary somatosensory cortex (SI) has two significant discontinuities: the hands and face map onto adjacent regions in SI, as do the feet and genitalia. It has been proposed that these conjunctions in SI result from coincident sources of stimulation in the fetal position, where the hands frequently touch the face, and the feet the genitalia. Computer modeling using a Hebbian variant of the self-organizing Kohonen net is consistent with this proposal. However, recent work reveals that the genital representation in SI for cutaneous sensations (as opposed to tumescence) is continuous with that of the lower trunk and thigh. This result, in conjunction with reports of separate face innervation and its earlier onset of sensory function, compared to that of the rest of the body, allows a reappraisal of homuncular organization. It is proposed that the somatosensory homunculus comprises two distinct somatotopic regions: the face representation and that of the rest of the body. Principles of self-organization do not account satisfactorily for the overall homuncular map. These results may serve to alert computational modelers that intrinsic developmental factors can override simple rules of plasticity.


2020 ◽  
Vol 4 (3) ◽  
pp. 557-562
Author(s):  
Rindi Novitri Antika

This dedication activity aims to increase the knowledge of adolescents about Propionibacterium acnes bacteria for skin health, especially acne, and form the awareness of adolescents to maintain and enhance the level of personal health. The specific target is for teenagers to be aware of how to maintain healthy skin, especially the face, remembering that adolescence is a period where their activities are very high, like activities outside the home, often forgetting to clean faces exposed to dust and dirt, as well as hormonal changes that affect the physiological of the body. This activity also provides knowledge about the bacteria Propionibacterium acnes, ranging from understanding, characteristics to the mechanism of action of bacteria. The methods and techniques of socialization consist of stages: planning, action, and reflection. 1) planning is carried out with the preparation and socialization of the program; 2) action, i.e. the implementation phase of the service, begins with exposure to the bacterium Propionibacterium acnes, the mechanism of action of the bacteria and its relation to skin health, then proceed with a question and answer session; 3) reflection, done by analyzing the participants' responses to the activities that have been carried out.


2020 ◽  
Vol 99 (4) ◽  
pp. 379-383
Author(s):  
Vasily N. Afonyushkin ◽  
N. A. Donchenko ◽  
Ju. N. Kozlova ◽  
N. A. Davidova ◽  
V. Yu. Koptev ◽  
...  

Pseudomonas aeruginosa is a widely represented species of bacteria possessing of a pathogenic potential. This infectious agent is causing wound infections, fibrotic cystitis, fibrosing pneumonia, bacterial sepsis, etc. The microorganism is highly resistant to antiseptics, disinfectants, immune system responses of the body. The responses of a quorum sense of this kind of bacteria ensure the inclusion of many pathogenicity factors. The analysis of the scientific literature made it possible to formulate four questions concerning the role of biofilms for the adaptation of P. aeruginosa to adverse environmental factors: Is another person appears to be predominantly of a source an etiological agent or the source of P. aeruginosa infection in the environment? Does the formation of biofilms influence on the antibiotic resistance? How the antagonistic activity of microorganisms is realized in biofilm form? What is the main function of biofilms in the functioning of bacteria? A hypothesis has been put forward the effect of biofilms on the increase of antibiotic resistance of bacteria and, in particular, P. aeruginosa to be secondary in charcter. It is more likely a biofilmboth to fulfill the function of storing nutrients and provide topical competition in the face of food scarcity. In connection with the incompatibility of the molecular radii of most antibiotics and pores in biofilm, biofilm is doubtful to be capable of performing a barrier function for protecting against antibiotics. However, with respect to antibodies and immunocompetent cells, the barrier function is beyond doubt. The biofilm is more likely to fulfill the function of storing nutrients and providing topical competition in conditions of scarcity of food resources.


2020 ◽  
Author(s):  
Jaimie Krems ◽  
Steven L. Neuberg

Heavier bodies—particularly female bodies—are stigmatized. Such fat stigma is pervasive, painful to experience, and may even facilitate weight gain, thereby perpetuating the obesity-stigma cycle. Leveraging research on functionally distinct forms of fat (deposited on different parts of the body), we propose that body shape plays an important but largely underappreciated role in fat stigma, above and beyond fat amount. Across three samples varying in participant ethnicity (White and Black Americans) and nation (U.S., India), patterns of fat stigma reveal that, as hypothesized, participants differently stigmatized equally-overweight or -obese female targets as a function of target shape, sometimes even more strongly stigmatizing targets with less rather than more body mass. Such findings suggest value in updating our understanding of fat stigma to include body shape and in querying a predominating, but often implicit, theoretical assumption that people simply view all fat as bad (and more fat as worse).


Author(s):  
Rajendra Pai N. ◽  
U. Govindaraju

Ayurveda in its principle has given importance to individualistic approach rather than generalize. Application of this examination can be clearly seem like even though two patients suffering from same disease, the treatment modality may change depending upon the results of Dashvidha Pariksha. Prakruti and Pramana both used in Dashvidha Pariksha. Both determine the health of the individual and Bala (strength) of Rogi (Patient). Ayurveda followed Swa-angula Pramana as the unit of measurement for measuring the different parts of the body which is prime step assessing patient before treatment. Sushruta and Charaka had stated different Angula Pramana of each Pratyanga (body parts). Specificity is the characteristic property of Swa-angula Pramana. This can be applicable in present era for example artificial limbs. A scientific research includes collection, compilation, analysis and lastly scrutiny of entire findings to arrive at a conclusion. Study of Pramana and its relation with Prakruti was conducted in 1000 volunteers using Prakruti Parkishan proforma with an objective of evaluation of Anguli Pramana in various Prakriti. It was observed co-relating Pramana in each Prakruti and Granthokta Pramana that there is no vast difference in measurement of head, upper limb and lower limb. The observational study shows closer relation of features with classical texts.


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