Technical notes on routine TEM procedures

1987 ◽  
Vol 45 ◽  
pp. 630-631
Author(s):  
K. Chien ◽  
R.L. Van de Velde ◽  
R.C. Heusser
Keyword(s):  
Epoxy Resins ◽  
Pathology Laboratory ◽  
Silicone Fluid ◽  
Image Position

Sectioning quality of epoxy resins can be improved by the addition of a 1% silicone 200 fluid (Dow Corning), however this produces a softer block. To compensate, a harder plastic has been used for embedding various tissues encountered in our pathology laboratory. Exact amounts of the plastic mixture can be directly made up for embedding as shown: The chart reveals a Poly/Bed 812 (WPE 145) to anhydride ratio of 1:0.7 and a NMA to DDSA ratio of 7:3. 1% silicone fluid is added to above mixtures.Due to impurities within the DDSA and NMA, the polymerized epoxy blocks vary in darkness and appear to affect sectioning quality. After discussing this problem with Polysciences Inc., they have agreed to purify their anhydrides in an effort to standardize the consistency of the plastic.

Stain contamination and embedding in electron microscopy

1986 ◽  
Vol 44 ◽  
pp. 50-53
Author(s):  
Hilton H. Mollenhauer
Keyword(s):  
Electron Microscopy ◽  
Information Retrieval ◽  
Epoxy Resins ◽  
Image Contrast ◽  
Sample Preservation ◽  
Factors Associated ◽  
Amount Of Information ◽  
Electron Microscopical ◽  
Contrast Information

Many factors (e.g., resolution of microscope, type of tissue, and preparation of sample) affect electron microscopical images and alter the amount of information that can be retrieved from a specimen. Of interest in this report are those factors associated with the evaluation of epoxy embedded tissues. In this context, informational retrieval is dependant, in part, on the ability to “see” sample detail (e.g., contrast) and, in part, on tue quality of sample preservation. Two aspects of this problem will be discussed: 1) epoxy resins and their effect on image contrast, information retrieval, and sample preservation; and 2) the interaction between some stains commonly used for enhancing contrast and information retrieval.

scholarly journals Biobanking for cancer research: Preservation of tissue integrity – Some technical considerations

2012 ◽  
Vol 01 (02) ◽  
pp. 130-138 ◽  
Author(s):  
Anita Mahadevan ◽  
S.K. Shankar
Keyword(s):  
Biomarker Discovery ◽  
Molecular Diagnostic ◽  
Diagnostic Tools ◽  
Future Research ◽  
Pathology Laboratory ◽  
Tissue Integrity ◽  
Oncology Research ◽  
Genomic Studies ◽  
And Storage

Abstract Biobanking and biomarker discovery have become an integral part of neuro-oncology research. Towards achieving this end, the essential requirement is optimizing methods of tissue preservation of human tissues removed at surgery for diagnostic purposes and banking them for subserving future research. Owing to recent advances in molecular diagnostic tools, this clinical material has become a precious source for proteomic and genomic studies. The advent of biotechnological tools such as microarray, proteomics, and genomics has made it essential to preserve not just morphology but also the quality of nucleic acids and proteins, changing the traditional workflow of a pathology laboratory. It is therefore essential to develop simple technologies for tissue fixation and storage ensure that receptor and molecular integrity is reasonably maintained. Knowledge of the basic chemistry of tissue fixatives, the biochemical changes that take place in biological material by utilizing different techniques of fixation is essential while undertaking molecular, genomic, and proteomic studies on fresh and archival tissues.

Coping strategies and quality of life in schizophrenia outpatients treated by Psychopharmacs - cross-sectional study

2016 ◽  
Vol 33 (S1) ◽  
pp. s252-s253
Author(s):  
M. Holubova ◽  
J. Prasko ◽  
R. Hruby ◽  
D. Kamaradova ◽  
M. Ociskova ◽  
...  
Keyword(s):  
Quality Of Life ◽  
Regression Analysis ◽  
Coping Strategies ◽  
Positive Coping ◽  
Negative Coping ◽  
Image Position ◽  
Self Instruction ◽  
And Coping ◽  
The Relationship

IntroductionThe modern psychiatric view of schizophrenia spectrum disorders and their treatment has led to an increasing focus on coping strategies and quality of life of these patients.ObjectivesUnderstanding the relationship between quality of life and coping strategies can help in finding those coping strategies that enhance the quality of life. It is important to study the inner experience and striving of patients because of connection with well-being and treatment adherence.Aims:In the present study, the authors examined the relationship between demographic data, the severity of symptoms, coping strategies, and quality of life in psychotic outpatients.MethodsPsychiatric outpatients who met ICD-10 criteria for a psychotic disorder (schizophrenia, schizoaffective disorder, or delusional disorder) were recruited in the study. Questionnaires measuring the coping strategies (SVF-78), the quality of life (Q-LES-Q), and symptoms severity (objective and subjective clinical global impression–objCGI; subjCGI) were assessed. Data were analysed using one-way ANOVA, Mann-Whitney U-test, Pearson and Spearman correlation coefficients, and multiple regression analysis.ResultsOne hundred and nine psychotic outpatients were included in the study. The QoL was significantly related to the Positive and Negative coping strategies. The severity of disorder highly negatively correlated with the QoL score. Stepwise regression analysis showed that symptoms severity (subjCGI), Positive coping strategies (especially Positive Self-instruction), Difference between the objCGI and subjCGI and Negative coping strategies explain totally 53.8% of variance of the QoL (Tables 1–3).Table 1Description of the sample, demographic and clinical data. Table 2Description of using coping strategies and quality of life in schizophrenic outpatients. Average use of coping 40-60 T-score, more than 60 overusing, less than 40 reduced use of coping strategy.Table 3Correlations between quality of life and coping strategies. *P < 0.05; **P < 0.01; ***P < 0.001.ConclusionsOur study suggests the importance of utilizing the Positive coping strategies in improving the quality of life in patients with psychotic disorders.Disclosure of interestThe authors have not supplied their declaration of competing interest.

Prevalence of restless legs syndrome in professional cyclists

2016 ◽  
Vol 33 (S1) ◽  
pp. s267-s268
Author(s):  
M. Kwiatkowski ◽  
U. Cwalina ◽  
P. Kosielski ◽  
K. Simonienko ◽  
P. Wierzbinski ◽  
...  
Keyword(s):  
Restless Legs Syndrome ◽  
Sport Performance ◽  
Age Group ◽  
Professional Performance ◽  
Restless Legs ◽  
Professional Cyclist ◽  
Item Questionnaire ◽  
Competing Interest ◽  
Image Position

IntroductionRestless legs syndrome (RLS) is a sleep disorder affecting a significant part of general population. Clinically RLS is characterized by abnormal sensations of irresistible urge to move legs, becoming worse at night, and frequently causing insomnia. Consequences of RLS include daytime dysfunction, depressed mood, anxiety and decreased quality of life.ObjectivesDetermine prevalence of RLS in professional cyclists.AimsThe aim of this study was to examine the prevalence of RLS in professional cyclists. According to our knowledge this is the first research of RLS in professional cyclists.MethodsA total of 43 professional cyclists were asked to complete 4-item questionnaire based on IRLSSG Diagnostic Criteria for RLS. Data from questionnaire was compared to antropometric measurements, epidemiologic data, age of starting trainings and total years of professional performance.ResultsThe results indicated that prevalence of RLS in professional cyclists is 14% and is higher than expected in this age group. We found statistically significant relationship between RLS and age (P < 0,001) and years of experience as a professional cyclist (P < 0,001). Results revealed no significant differences between genders (Table 1).Table 1 ConclusionsRLS seems to be a common problem in professional cyclists. It can affect sport performance. Future efforts to estimate the influence of RLS on professional sport performance should be considered.Disclosure of interestThe authors have not supplied their declaration of competing interest.

scholarly journals Gambaran Tingkat Kepuasan Pasien terhadap Pelayanan FNAB di Laboratorium Patologi Anatomi RSUD Abdul Wahab Sjahranie Samarinda Tahun 2018

2019 ◽  
pp. 198-206
Author(s):  
Simanjuntak Mayro ◽  
Eko Nugroho ◽  
Oswald L Simatupang
Keyword(s):  
Quality Of Service ◽  
Patient Satisfaction ◽  
Health Services ◽  
Sampling Technique ◽  
Primary Data ◽  
Pathology Laboratory ◽  
Purposive Sampling ◽  
Expectation Gap ◽  
Anatomical Pathology

Abstrak Kepuasan pasien adalah suatu tingkat perasaan pasien yang timbul akibat dari kinerja layanan kesehatan yang diperoleh setelah pasien membandingkan dengan apa yang diharapkannya, sehingga kepuasan pasien bergantung pada kualitas pelayanan yang diterimanya. Untuk menilai kualitas pelayanan terdapat 5 dimensi yang dapat diukur, yaitu keandalan (reliability), daya tanggap (responsiveness), jaminan (assurance), empati (empathy), dan keberwujudan (tangible). Makin baik kualitas pelayanan yang diberikan, maka akan semakin tinggi tingkat kepuasan pasien. Tujuannya untuk mengetahui tingkat kepuasan pasien terhadap pelayanan di laboratorium patologi anatomi RSUD Abdul Wahab Sjahranie Samarinda. Penelitian deskriptif dilakukan dengan teknik purposive sampling pada pasien yang datang ke Laboratorium Patologi Anatomi RSUD Abdul Wahab Sjahranie, Samarinda, yang memenuhi kriteria. Data yang peroleh berasal dari data primer dengan menggunakan kuesioner yang dikerjakan secara terbimbing. Terdapat kesenjangan (gap) harapan sebesar -0,6 untuk dimensi keandalan, -0,2 untuk dimensi daya tanggap, -0,2 untuk dimensi jaminan, -0,1 dimensi empati, dan -0,9 untuk dimensi keberwujudan. Tingkat kepuasan pasien adalah 96% responden puas dan 4% responden tidak puas. Kata kunci: kepuasan pasien, pelayanan kesehatan, keandalan, daya tanggap, jaminan Abstract Patient satisfaction is a level of patient's feelings rising from the performance of health services that are obtained after the patient compares it with what he expected, so that patient satisfaction depends on the quality of service received. To assess service quality there are 5 dimensions that can be measured, namely reliability, responsiveness, assurance, empathy, and tangible. The better quality of service provided, the higher the level of patient satisfaction. To determine the level of patient satisfaction with services in the anatomical pathology laboratory of the Abdul Wahab Sjahranie Hospital, Samarinda. Descriptive study was conducted by purposive sampling technique carried out on 50 patients who came to the Anatomical Pathology Laboratory of Abdul Wahab Sjahranie Hospital, in Samarinda that met the criteria. The data obtained comes from primary data using a questionnaire that is done in a guided manner. There was an expectation gap of -0.6 for reliability dimensions, -0.2 for responsiveness dimensions, -0,2 for assurance dimensions, -0.1 empathy dimensions and -0,9 for tangible dimensions. The level of patient satisfaction was 96% of respondents were satisfied and 4% of respondents were dissatisfied. Keywords: patient satisfaction, health services, reliability, responsiveness, assurance

scholarly journals ANALISIS PENGUKURAN KUALITAS PELAYANAN KONSUMEN DENGAN METODE SERVQUAL

2021 ◽  
Vol 16 (2) ◽  
Author(s):  
Dhiana Ekowati ◽  
Arsyad Fadhlur Rahman
Keyword(s):  
Quality Of Service ◽  
Data Analysis ◽  
Service Quality ◽  
Service Providers ◽  
Clinical Pathology ◽  
Pathology Laboratory ◽  
Laboratory Installation ◽  
Health Institution ◽  
Consumer Expectations

The hospital is a health institution that is engaged in services. The quality of a service will encourage consumers to form strong bonds with the company. If the service received exceeds consumer expectations, service quality is perceived as ideal quality. Conversely, if the service received is lower than expected, the quality of the service is perceived as poor. Thus, whether the quality of service is good or not depends on the ability of service providers to consistently meet consumer expectations. This study aims to measure the service quality of the Clinical Pathology Laboratory Installation of RSUP Sarjito Yogyakarta using the Servqual Method.       The subjects of this study were consumers of the Clinical Pathology Laboratory Installation of the Sarjito Hospital Yogyakarta, while the object of this study was the quality of service for the Clinical Pathology Laboratory Installation of the Sarjito Hospital Yogyakarta. The sample of this research is 99 respondents. The data analysis of this research uses SERVQUAL analysis.       The results of this study indicate that the service quality of the Clinical Pathology Laboratory Dr. Sardjito Yogyakarta provides satisfaction to consumers. Keywoard: Quality of Service, SERVQUAL.

scholarly journals Effect of Storage Condition on Seed Quality of Some Dhaincha (Sesbania spp.) Accessions

2016 ◽  
Vol 14 (1) ◽  
pp. 83-91
Author(s):  
M Towhidul Karim ◽  
Fazlul Haque ◽  
A K M Golam Sarwar ◽  
M Ayub Ali
Keyword(s):  
Room Temperature ◽  
Seed Quality ◽  
Storage Condition ◽  
Storage Conditions ◽  
Pathology Laboratory ◽  
Seed Pathology ◽  
Vigour Index ◽  
Long Time ◽  
Fusarium Sp

An experiment was conducted at the Seed Pathology Laboratory, Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh, to understand the effect of storage conditions on seed quality of different accessions of dhaincha (Sesbania spp.). Seeds of twenty accessions were collected and stored in a refrigerator (4°C) and laboratory at normal room temperature (25-30°C). Sprouting (%), germination (%), vigour index and the incidence of seed-borne pathogens were recorded following ISTA procedures. Germination and emergence were higher when seeds were stored in the refrigerator than those in room temperature. The vigour index was also high when the seeds were stored in a refrigerator. Fungi belonging to genera Aspergillus, Fusarium, Rhizopus, and Penicillium were associated with seeds of the different accessions of dhaincha. Seed borne infection of Fusarium sp. was highest (14%) in G-13 when seeds were stored at room temperature. It was evident from the results that the quality of the seeds of dhaincha accessions was retained for a long time when they were stored at low (around 4°C) temperature.The Agriculturists 2016; 14(1) 83-91

Experiment Study on Nd: YAG Pulsed Laser Cutting Carbon Fibers Composites

2011 ◽  
Vol 464 ◽  
pp. 510-513
Author(s):  
Tao Ge ◽  
Yong Kang Zhang ◽  
Xiao Meng Chu
Keyword(s):  
Laser Pulse ◽  
Carbon Fibers ◽  
Laser Cutting ◽  
Epoxy Resins ◽  
Laser Pulse Energy ◽  
Cutting Speed ◽  
Pulsed Laser ◽  
Evaluation Index ◽  
Experiment Study

Nd:YAG pulsed laser cutting carbon fibers reinforced epoxy resins composites was studied. HAZ and kerf width was made as evaluation index, as well as laser pulse energy, cutting speed, pulse duration and assistant gas pressure was made as controlled parameters. Ideal quality of cutting can be achieved by adjusting those parameters. The effect of each parameter was analyzed.

scholarly journals RESIN FLOW SIMULATION DURING THE RTM METHOD OF COMPOSITES PRODUCTION

2021 ◽  
Vol 13 (3) ◽  
pp. 125-133
Author(s):  
Pawel Paździor ◽  
◽  
Miroslaw Szczepanik ◽  
Keyword(s):  
Carbon Fiber ◽  
Injection Molding ◽  
Epoxy Resins ◽  
Flow Simulation ◽  
Resin Flow ◽  
Serial Production ◽  
Transfer Molding ◽  
Molding Method ◽  
Plastic Injection

Processes of plastic injection molding are often under analyzes in industry and science. Many of these considerations apply to epoxy resins with additional reinforcement, often with glass or carbon fiber inside the closed mould. The simulations of injection molding processes in the production of composite elements is not as common, as thermoplasts. Hence the idea to carry out the work described in this article. The RTM (Resin Transfer Molding) method is dedicated to serial production with the possibility of producing visual carbon fiber elements for aesthetic reasons. Simulations can help to better refine the products. This allows to take appropriate precautions and solve many issues before implementation. The article presents possible situations that could occur in real conditions. Various shapes models were prepared as basis of the numerical calculations. The analyses highlighting the possible issues were performed. The influence of resin pressure and flow rate on the final product was also considered. The aim was to present the characteristic phenomena and their causes that often occur in reality to technologists working with the RTM. Conclusions related to the work carried out are included. Based on the analyzes and conclusions drawn, it is possible to improve the quality of production processes.

scholarly journals A TEM study of GaN grown by ELO on (0001) 6H-SiC

2000 ◽  
Vol 5 (S1) ◽  
pp. 76-82
Author(s):  
P. Ruterana ◽  
B. Beaumont ◽  
P. Gibart ◽  
Y. Melnik
Keyword(s):  
Lattice Mismatch ◽  
Early Stage ◽  
Vapour Phase ◽  
Epitaxial Layers ◽  
Prismatic Plane ◽  
Vapour Phase Epitaxy ◽  
Hexagonal Shape ◽  
To Come ◽  
Image Position

The misfit between GaN and 6H-SiC is 3.5 % instead of 16 % with sapphire, the epitaxial layers have similar densities of defects on both substrates. Moreover, the lattice mismatch between AlN and 6H-SiC is only 1%. Therefore, epitaxial layer overgrowth (ELO) of GaN on AlN/6H-SiC could be a route to further improve the quality of epitaxial layers. AlN has been grown by Halide Vapour Phase Epitaxy (HVPE) on (0001) 6H-SiC, thereafter a dielectric SiO2 mask was deposited and circular openings were made by standard photolithography and reactive ion etching. We have examined GaN layers at an early stage of coalescence in order to identify which dislocations bend and try to understand why. The analysed islands have always the same hexagonal shape, limited by facets. The a type dislocations are found to fold many times from basal to the prismatic plane, whereas when a+c dislocations bend to the basal plane, they were not seen to come back to a prismatic one.

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