Secondary Ion Mass Spectrometry (SIMS) Microscopy as an Imaging Tool for Physiological Studies II. SIMS Microscopy of Plant Tissues

1996 ◽  
Vol 2 (3) ◽  
pp. 129-136 ◽  
Author(s):  
Nicole Grignon ◽  
Sylvain Halpern ◽  
Josette Jeusset ◽  
Philippe Fragu
Keyword(s):  
Cell Wall ◽  
Large Scale ◽  
Secondary Ion Mass Spectrometry ◽  
A Priori ◽  
Plant Cells ◽  
Limiting Factor ◽  
Imaging Tool ◽  
Rigid Cell Wall ◽  
Large Central Vacuole ◽  
Small Solute

Serious difficulties are encountered when SIMS analysis is applied to plant cells because of the cells' basic organization. In most plant cells, the cytoplasm is present as a thin layer that surrounds a large central vacuole, and is surrounded externally by a porous semi-rigid cell wall. Due to the high internal hydrostatic pressure typical of plant cells, large-scale solute redistribution may occur when tissues are excised. Relatively small solute decompartmentation is sufficient to collapse the native solute gradients between the cytoplasm and the adjacent compartments, due to the small volume of the former. For these reasons, most of the SIMS analyses in plant cells have been performed on elements bound to non-diffusible structures such as proteins, cell wall polymers, or in dry seeds. Sample preparation remains a limiting factor when imaging the distribution of soluble compounds. Cryotechniques have generated considerable interest to circumvent these problems. Cryofixation followed by cryosectioning would a priori be the best procedure, but encouraging results indicate that cryofixation followed by cryosubstitution is an interesting alternative.

scholarly journals Widespread genome instability in Solanum tuberosum plants regenerated from protoplasts

2018 ◽  
Author(s):  
Michelle Fossi ◽  
Luca Comai
Keyword(s):  
Tissue Culture ◽  
Cell Wall ◽  
Copy Number ◽  
Large Scale ◽  
Genome Instability ◽  
Crop Improvement ◽  
Plant Cells ◽  
Read Depth ◽  
Control Set ◽  
Copy Number Changes

AbstractNon-transgenic genome editing in regenerable protoplasts, cell-wall free plant cells, could revolutionize crop improvement because it reduces regulatory and technical complexity. But, plant tissue culture is known to engender frequent unwanted variation. To evaluate the contribution of genome instability to this phenomenon, we analyzed large scale copy number changes in potatoes regenerated from protoplasts by comparison of Illumina read depth. While a control set of plants that had been propagated by cuttings displayed no changes, the protoplast regenerants were affected by pervasive aneuploidy. In addition, certain chromosomes displayed segmental deletions and duplications ranging from one to many. Resampling the same plant found different dosage profiles in different leaves, indicating frequent persistence of instability.

Sites of Adsorption of the Bacteriophages T3 and T5 on the Wall of Escherichia Coli B.

1967 ◽  
Vol 25 ◽  
pp. 96-97
Author(s):  
Manfred E. Bayer
Keyword(s):  
Escherichia Coli ◽  
Cell Wall ◽  
Electron Microscope ◽  
Uranyl Acetate ◽  
E Coli ◽  
Receptor Sites ◽  
Rigid Cell Wall ◽  
Host Cell Surface ◽  
Bacterial Viruses ◽  
Escherichia Coli B

Bacterial viruses adsorb specifically to receptors on the host cell surface. Although the chemical composition of some of the cell wall receptors for bacteriophages of the T-series has been described and the number of receptor sites has been estimated to be 150 to 300 per E. coli cell, the localization of the sites on the bacterial wall has been unknown.When logarithmically growing cells of E. coli are transferred into a medium containing 20% sucrose, the cells plasmolize: the protoplast shrinks and becomes separated from the somewhat rigid cell wall. When these cells are fixed in 8% Formaldehyde, post-fixed in OsO4/uranyl acetate, embedded in Vestopal W, then cut in an ultramicrotome and observed with the electron microscope, the separation of protoplast and wall becomes clearly visible, (Fig. 1, 2). At a number of locations however, the protoplasmic membrane adheres to the wall even under the considerable pull of the shrinking protoplast. Thus numerous connecting bridges are maintained between protoplast and cell wall. Estimations of the total number of such wall/membrane associations yield a number of about 300 per cell.

Enrichment of vitronectin- and fibronectin-like proteins in NaCI-adapted plant cells and evidence for their involvement in plasma membrane-cell wall adhesion

1993 ◽  
Vol 3 (5) ◽  
pp. 637-646 ◽  
Author(s):  
Jian-Kang Zhu ◽  
Jun Shi ◽  
Utpal Singh ◽  
Sarah E. Wyatt ◽  
Ray A. Bressan ◽  
...  
Keyword(s):  
Cell Wall ◽  
Plasma Membrane ◽  
Plant Cells ◽  
Membrane Cell

Recent Developments in the Downstream Processing of Phycobiliproteins from Algae: A Review

2021 ◽  
Vol 06 ◽  
Author(s):  
Ayekpam Chandralekha Devi ◽  
G. K. Hamsavi ◽  
Simran Sahota ◽  
Rochak Mittal ◽  
Hrishikesh A. Tavanandi ◽  
...  
Keyword(s):  
Cell Wall ◽  
Large Scale ◽  
Downstream Processing ◽  
Review Article ◽  
Current Status ◽  
Wall Structure ◽  
Scale Production ◽  
Cell Wall Disruption ◽  
Recent Developments ◽  
Macro Algae

Abstract: Algae (both micro and macro) have gained huge attention in the recent past for their high commercial value products. They are the source of various biomolecules of commercial applications ranging from nutraceuticals to fuels. Phycobiliproteins are one such high value low volume compounds which are mainly obtained from micro and macro algae. In order to tap the bioresource, a significant amount of work has been carried out for large scale production of algal biomass. However, work on downstream processing aspects of phycobiliproteins (PBPs) from algae is scarce, especially in case of macroalgae. There are several difficulties in cell wall disruption of both micro and macro algae because of their cell wall structure and compositions. At the same time, there are several challenges in the purification of phycobiliproteins. The current review article focuses on the recent developments in downstream processing of phycobiliproteins (mainly phycocyanins and phycoerythrins) from micro and macroalgae. The current status, the recent advancements and potential technologies (that are under development) are summarised in this review article besides providing future directions for the present research area.

scholarly journals An efficient direct screening system for microorganisms that activate plant immune responses based on plant–microbe interactions using cultured plant cells

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Mari Kurokawa ◽  
Masataka Nakano ◽  
Nobutaka Kitahata ◽  
Kazuyuki Kuchitsu ◽  
Toshiki Furuya
Keyword(s):  
Immune Responses ◽  
Plant Defense ◽  
Pseudomonas Syringae ◽  
Large Scale ◽  
Plant Cells ◽  
Defense Responses ◽  
Root Tip ◽  
General Strategy ◽  
Plant Defense Responses ◽  
Microbe Interactions

AbstractMicroorganisms that activate plant immune responses have attracted considerable attention as potential biocontrol agents in agriculture because they could reduce agrochemical use. However, conventional methods to screen for such microorganisms using whole plants and pathogens are generally laborious and time consuming. Here, we describe a general strategy using cultured plant cells to identify microorganisms that activate plant defense responses based on plant–microbe interactions. Microbial cells were incubated with tobacco BY-2 cells, followed by treatment with cryptogein, a proteinaceous elicitor of tobacco immune responses secreted by an oomycete. Cryptogein-induced production of reactive oxygen species (ROS) in BY-2 cells served as a marker to evaluate the potential of microorganisms to activate plant defense responses. Twenty-nine bacterial strains isolated from the interior of Brassica rapa var. perviridis plants were screened, and 8 strains that enhanced cryptogein-induced ROS production in BY-2 cells were selected. Following application of these strains to the root tip of Arabidopsis seedlings, two strains, Delftia sp. BR1R-2 and Arthrobacter sp. BR2S-6, were found to induce whole-plant resistance to bacterial pathogens (Pseudomonas syringae pv. tomato DC3000 and Pectobacterium carotovora subsp. carotovora NBRC 14082). Pathogen-induced expression of plant defense-related genes (PR-1, PR-5, and PDF1.2) was enhanced by the pretreatment with strain BR1R-2. This cell–cell interaction-based platform is readily applicable to large-scale screening for microorganisms that enhance plant defense responses under various environmental conditions.

Current State of (Dis)Integration: Public Health and Fusion Centers

2020 ◽  
Vol 17 (2) ◽  
Author(s):  
Cody Minks ◽  
Anke Richter
Keyword(s):  
Public Health ◽  
Law Enforcement ◽  
Information Sharing ◽  
Homeland Security ◽  
Large Scale ◽  
Ad Hoc ◽  
Limiting Factor ◽  
Fusion Centers ◽  
Public Health Emergencies ◽  
Public Health Information

AbstractObjectiveResponding to large-scale public health emergencies relies heavily on planning and collaboration between law enforcement and public health officials. This study examines the current level of information sharing and integration between these domains by measuring the inclusion of public health in the law enforcement functions of fusion centers.MethodsSurvey of all fusion centers, with a 29.9% response rate.ResultsOnly one of the 23 responding fusion centers had true public health inclusion, a decrease from research conducted in 2007. Information sharing is primarily limited to information flowing out of the fusion center, with little public health information coming in. Most of the collaboration is done on a personal, informal, ad-hoc basis. There remains a large misunderstanding of roles, capabilities, and regulations by all parties (fusion centers and public health). The majority of the parties appear to be willing to work together, but there but there is no forward momentum to make these desires a reality. Funding and staffing issues seem to be the limiting factor for integration.ConclusionThese problems need to be urgently addressed to increase public health preparedness and enable a decisive and beneficial response to public health emergencies involving a homeland security response.

Towards A Multi-FPGA Infrared Simulator

2007 ◽  
Vol 4 (4) ◽  
pp. 343-355 ◽  
Author(s):  
Vinay Sriram ◽  
David Kearney
Keyword(s):  
Homeland Security ◽  
Reconfigurable Computing ◽  
High Speed ◽  
High Performance ◽  
Large Scale ◽  
Computation Time ◽  
Ccd Camera ◽  
Hardware Acceleration ◽  
Limiting Factor ◽  
Scene Simulation

High speed infrared (IR) scene simulation is used extensively in defense and homeland security to test sensitivity of IR cameras and accuracy of IR threat detection and tracking algorithms used commonly in IR missile approach warning systems (MAWS). A typical MAWS requires an input scene rate of over 100 scenes/second. Infrared scene simulations typically take 32 minutes to simulate a single IR scene that accounts for effects of atmospheric turbulence, refraction, optical blurring and charge-coupled device (CCD) camera electronic noise on a Pentium 4 (2.8GHz) dual core processor [7]. Thus, in IR scene simulation, the processing power of modern computers is a limiting factor. In this paper we report our research to accelerate IR scene simulation using high performance reconfigurable computing. We constructed a multi Field Programmable Gate Array (FPGA) hardware acceleration platform and accelerated a key computationally intensive IR algorithm over the hardware acceleration platform. We were successful in reducing the computation time of IR scene simulation by over 36%. This research acts as a unique case study for accelerating large scale defense simulations using a high performance multi-FPGA reconfigurable computer.

EARRINGS: an efficient and accurate adapter trimmer entails no a priori adapter sequences

2021 ◽  
Author(s):  
Ting-Hsuan Wang ◽  
Cheng-Ching Huang ◽  
Jui-Hung Hung
Keyword(s):  
Open Source Software ◽  
Large Scale ◽  
A Priori ◽  
Supplementary Information ◽  
Supplementary Data ◽  
Comparable Accuracy ◽  
Meta Analyses ◽  
Next Generation Sequencing Ngs ◽  
Adapter Trimming ◽  
Generation Sequencing

Abstract Motivation Cross-sample comparisons or large-scale meta-analyses based on the next generation sequencing (NGS) involve replicable and universal data preprocessing, including removing adapter fragments in contaminated reads (i.e. adapter trimming). While modern adapter trimmers require users to provide candidate adapter sequences for each sample, which are sometimes unavailable or falsely documented in the repositories (such as GEO or SRA), large-scale meta-analyses are therefore jeopardized by suboptimal adapter trimming. Results Here we introduce a set of fast and accurate adapter detection and trimming algorithms that entail no a priori adapter sequences. These algorithms were implemented in modern C++ with SIMD and multithreading to accelerate its speed. Our experiments and benchmarks show that the implementation (i.e. EARRINGS), without being given any hint of adapter sequences, can reach comparable accuracy and higher throughput than that of existing adapter trimmers. EARRINGS is particularly useful in meta-analyses of a large batch of datasets and can be incorporated in any sequence analysis pipelines in all scales. Availability and implementation EARRINGS is open-source software and is available at https://github.com/jhhung/EARRINGS. Supplementary information Supplementary data are available at Bioinformatics online.

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