Degradability characteristics and Intestinal protein apparent digestibility of Iranian soybean and cottonseed meals as assessed by the mobile nylon bag technique

2002 ◽  
Vol 2002 ◽  
pp. 147-147
Author(s):  
M Danesh Mesgaran

Ruminal degradability of feed protein is an important factor when assessing feed protein value according to the modern ruminant feed evaluation systems. In addition, the worth of any food protein to ruminant animals depends on the how the protein is digested in the small intestine. The work described in this summary assessed the in situ degradability and intestinal apparent digestibility of dry matter and protein, using ruminal and intestinal mobile nylon bags technique, of Iranian soybean (S) and cottonseed (C) meals.

2002 ◽  
Vol 2002 ◽  
pp. 144-144
Author(s):  
A. Taghizadeh ◽  
J. shoja ◽  
G. Moghaddam ◽  
H. Jhonmohammadi ◽  
P. Yasan

Values for crude protein (CP) and ruminally undegradable CP content are now required in feed evaluation systems currently used in North America (NRC,1989) and other organizations (Ørskov,1992).The objectives of the present study were to assess in situ ruminal degradability, fractional rates of digestion and the effective degradability of the dry matter (DM) and crude protein (CP) of alfalfa and red clover hays, selected randomly from dairy farms of Tabriz region in Iran on the male sheep rumen.


2016 ◽  
Vol 38 (2) ◽  
pp. 171 ◽  
Author(s):  
Renê Ferreira Costa ◽  
Daniel Ananias de Assis Pires ◽  
Marielly Maria Almeida Moura ◽  
José Avelino Santos Rodrigues ◽  
Vicente Ribeiro Rocha Júnior ◽  
...  

This study aimed to evaluate in situ degradability and degradation kinetics of DM, NDF and ADF of silage, with or without tannin in the grains. Two isogenic lines of grain sorghum (CMS-XS 114 with tannin and CMS-XS 165 without tannin) and two sorghum hybrids (BR-700 dual purpose with tannin and BR-601 forage without tannin) were ensiled; dried and ground silage samples were placed in nylon bags and introduced through the fistulas. After incubation for 6, 12, 24, 48, 72 and 96 hours, bags were taken for subsequent analysis of fibrous fractions. The experimental design was completely randomized with 4 replicates and 4 treatments and means compared by Tukey’s test at 5% probability. As for the DM degradation rate, silage of CMSXS165without tannin was superior. Silages of genotypes BR700 and CMSXS 114 with tannin showed the highest values of indigestible ADF (59.54 and 43.09%). Regarding the NDF, the potential degradation of silage of CMSXS165 line without tannin was superior. Tannin can reduce ruminal degradability of the dry matter and fibrous fractions. 


1999 ◽  
Vol 79 (3) ◽  
pp. 343-351 ◽  
Author(s):  
G. W. Mathison ◽  
R. Soofi-Siawash ◽  
E. K. Okine ◽  
J. Helm ◽  
P. Juskiw

Chemical composition and in situ ruminal degradability characteristics for 25 and 40 genotypes of barley straw were measured for crops grown in 1994 and 1995, respectively. Straw from semidwarf genotypes contained more crude protein and less neutral detergent fiber (NDF) than that from genotypes with medium straw length and effective ruminal degradability was 9% higher (P < 0.01). Straw from two-row genotypes contained 19% more crude protein (4.4 vs. 3.7%) and 3% less NDF (75.2 vs. 77.6%) than that from six-row genotypes and effective ruminal degradability was 6% higher (P < 0.01) when genotypes with medium-length straw only were considered. Genotypes with smooth awns had higher rates of degradation but contained less of the slowly degraded fraction than straw with rough awns when straws of medium straw length were compared (P < 0.05). Straw from hulless genotypes contained more (P = 0.006) NDF than that from covered varieties. Both ambient and soil temperature were positively related (P < 0.05) to effective ruminal degradability of straw, possibly because of associated greater leafiness when temperatures were higher and there was more light. It was concluded that it is possible to select barley genotypes that have superior straw nutritive value for ruminant animals. Key words: Barley, straw, degradability, cattle, genotype, environment


1985 ◽  
Vol 65 (3) ◽  
pp. 631-645 ◽  
Author(s):  
B. P. GLENN ◽  
D. G. ELY ◽  
S. GLENN ◽  
L. W. DOUGLASS ◽  
L. S. BULL ◽  
...  

Tall fescue (TF) and orchardgrass (OG) were fertilized with 0, 294 or 882 kg NH4NO3/ha and 0 or 833 kg K2SO4/ha to determine the effect on rates of apparent ruminal disappearance of herbage dry matter (DM), nitrogen (N) and sulfur (S) using the in situ nylon bag technique. Losses of water-soluble and filtrable DM, N and S differed significantly due to herbage species, NH4NO3 and K2SO4 fertilization levels. After 24-h ruminal incubation, there was no difference between herbages in residual DM, N or S expressed as a proportion of original nutrient. Increasing level of NH4NO3 fertilization reduced (P < 0.001) 24-h residual DM and N and increased (P < 0.001) residual S. Potassium sulfate fertilization reduced (P < 0.001) residual herbage S after 24-h incubation. Rates of insoluble herbage DM and N disappearance (kd) did not differ between herbages but increasing level of NH4NO3 fertilization increased (P < 0.001) insoluble DM and N disappearance rates. Rate of insoluble S disappearance was lower (P < 0.05) for TF than OG but unaffected by fertilization. Effective ruminal degradability (ERD) of DM (P < 0.01) and release (ERR) of N (P < 0.001) and S (P < 0.05) were lower for OG than TF. Increasing NH4NO3 fertilization increased (P < 0.001) ERD and ERR of DM and N, respectively. Furthermore, NH4NO3 fertilization reduced (P < 0.001) while K2SO4 application increased (P < 0.001) ERR of herbage S. Simple correlation coefficients between kd of DM, N and S and original herbage total N concentration were r = 0.59 (P < 0.001), r = 0.51 (P < 0.001) and r = 0.36 (P < 0.05), respectively. The relationships between ERR of herbage S and total N or nonprotein S concentration in herbage were r = −0.74 (P < 0.001) and r = 0.70 (P < 0.001), respectively. Key words: Tall fescue, orchardgrass, sulfur, nitrogen, ruminal in situ disappearance


2012 ◽  
Vol 10 (3) ◽  
pp. 285 ◽  
Author(s):  
Rafael Henrique De Tonissi e Buschinelli ◽  
Rita De Cássia Menchon Tramontini ◽  
Sérgio Tosi Cardim ◽  
Gabriel Daltoé de Almeida ◽  
Juliane Ribeiro ◽  
...  

O objetivo deste trabalho foi avaliar a degradabilidade ruminal da matéria seca e proteína bruta por meio da técnica in situ dos capins Brachiaria brizantha e Panicum maximum cortados aos 60 dias de idade, das silagens de milho, sorgo e milheto, dos fenos de coast cross, guandu e soja perene e do farelo de soja. Foram utilizados três novilhos com peso médio de 300 kg, fistulados e providos de cânulas ruminais. Os volumosos foram incubados em ordem decrescente de 72, 48, 24, 18, 12, 6, 3 e 0h, e o farelo de soja incubado nos tempos 48, 24, 18, 12, 6, 3 e 0h. Os resíduos dos sacos foram analisados quanto aos teores de matéria seca e proteína bruta. O farelo de soja apresentou alta degradabilidade efetiva para MS em função das frações solúveis (32,85%) e fração potencialmente degradável (65,75%). A silagem de sorgo apresentou elevada fração solúvel e potencialmente degradável se comparada às demais silagens. O feno de soja perene apresentou a maior degradabilidade de MS (80,42%), entre os volumosos avaliados. Para a proteína, destacam-se a baixa degradabilidade apresentada pelo feno de guandu (36,91%) e os demais volumosos apresentaram degradabilidade variando entre 64,50 a 74,56%.


2021 ◽  
Vol 66 (No. 8) ◽  
pp. 302-314
Author(s):  
Alemayehu Worku ◽  
Róbert Tóthi ◽  
Szilvia Orosz ◽  
Hedvig Fébel ◽  
László Kacsala ◽  
...  

This study was conducted using three multiparous non-lactating rumen-cannulated Holstein-Friesian dairy cows, with the objective of evaluating the in situ ruminal degradability and fermentation characteristics of novel mixtures of winter cereal and Italian ryegrass (Lolium multiflorum Lam.) plus winter cereal silages (mixture A: triticale, oats, barley and wheat; mixture B: triticale, barley and wheat; mixture C: Italian ryegrass and oats; mixture D: Italian ryegrass, oats, triticale, barley and wheat). The rumen fermentation study was conducted replacing the ensiled mixtures (experimental diets) with vetch-triticale haylage in a total mixed ration (control diet). It was found that the effective protein degradability at 0.08 rumen outflow rates was 80.6% (mixture A), 66.2% (mixture B), 79.7% (mixture C) and 79.3% (mixture D). The effective neutral detergent fibre (NDF) and acid detergent fibre (ADF) effective degradability at 0.08 rumen outflow rates was 18.0% and 17.7% (mixture A), 19.7% and 20.5% (mixture B), 19.1% and 17.0% (mixture C), and 15.2% and 14.6% (mixture D), respectively. Different dietary treatments did not change (P &gt; 0.05) the rumen fermentation characteristics as there was no difference (P &gt; 0.05) between control and experimental diets, and the inclusion of 40–55% Italian ryegrass (mixture C and D) did not cause any difference. These results suggest that the mixture of winter cereals and Italian ryegrass plus winter cereal-based silages had good potentially degradable dry matter, effective dry matter and effective protein degradability at 0.01, 0.05 and 0.08 rumen outflow rates without affecting the rumen environment maintaining neutral pH. The ensiled mixtures had a moderate level of potentially degradable NDF and ADF fractions.


2014 ◽  
Vol 59 (No. 6) ◽  
pp. 288-295 ◽  
Author(s):  
M. Chrenková ◽  
Z. Čerešňáková ◽  
M.R. Weisbjerg ◽  
Z. Formelová ◽  
M. Poláčiková ◽  
...  

Several methods were used for the evaluation of feed protein for rumen degradability and intestinal digestibility of rumen undegraded protein (RUP). The aim of this work was to explore the possibility of using the Cornell method of predicting the fraction RUP. An in situ method was adopted for estimation of degradability parameters (a, b, c) and effective degradable crude protein (EDCP), and five nitrogen fractions (A, B<sub>1</sub>, B<sub>2</sub>, B<sub>3</sub>, and C) were determined according to the Cornell Net Carbohydrate and Protein System (CNCPS). Fifty-one feedstuffs &ndash; 13 cereals grains, 5 legume seeds, 3 oilseeds, 11 oilseed by-products, 4 distillers dried grains with solubles (DDGS), and 15 silages (maize, lucerne, grass, and grass-clover) &ndash; were used in this work. The examined feedstuffs varied widely in nutrient composition. Fraction B<sub>1 </sub>(soluble true protein) in forages was small (2.5&ndash;5.7% of total N), but it varied to a large extent in concentrates (DDGS 0.9&ndash;1.2, legumes 46.5&ndash;63.7, oilseeds meal 17.1&ndash;51.8% of total N). Fraction B<sub>2</sub> represented a large proportion of the total protein in oil seed meals (44.3&ndash;82.6% of total N) and in DDGS (55.8&ndash;77.8% of total N), too. Fraction B<sub>3</sub> was relatively small (less than 10% of total N) in all feedstuffs and declined with increasing acid detergent insoluble nitrogen (ADIN) concentration. The concentration of ADIN fraction in feeds affected ruminal degradability. Lucerne silage, with a high content of ADIN (30.9% of total N), had a low effective crude protein (CP) degradability (57%). Correlation between EDCP and fraction A was r = 0.76. A weaker correlation (r = 0.67) was found between in situ parameter &ldquo;b&rdquo; and fractions B<sub>2 </sub>and B<sub>3 </sub>(r = 0.59), respectively. The results show that much more samples of all feed types should be analyzed to obtain results allowing a more exact prediction of CP degradability and RUP. &nbsp;


1988 ◽  
Vol 68 (3) ◽  
pp. 779-786 ◽  
Author(s):  
P. H. ROBINSON ◽  
J. J. KENNELLY

High-moisture barley (HMB) was ensiled in polyethylene silage bags. Treatments applied to 30% moisture HMB as a percentage of dry matter (DM) were: none, 1% ammonia, 2% ammonia, and 1.5% sulphur dioxide. A fifth treatment was the addition of 1.5% sulphur dioxide to 20% moisture HMB. Barley samples were ruminally incubated to estimate degradability of DM and nitrogen (N). Ruminally incubated bags were also introduced to the duodenal cannulae and collected in feces to estimate true whole-tract digestion. Sulphur dioxide treatment, or moisture content, had no influence on ruminal degradability, with the exception of increased degradation rate of potentially degradable N for 20 vs 30% HMB, or whole-tract true digestion. Ammoniation resulted in net reduction in rate of ruminal DM release due to shift of soluble DM to the potentially degradable fraction, and decrease in the potentially degradable fraction degradation rate. Ammoniation increased barley N content up to 44% with no change in rate of ruminal N release. Whole-tract true DM digestion was higher for ammoniated barley but N digestion was not influenced. Sulphur dioxide appears to offer no advantages beyond grain preservation, whereas ammoniation offers potential by reducting rate of ruminal DM release, and providing a source of slow release nonprotein N, without affecting post-ruminal digestibility. Key words: High-moisture barley, ammonia, sulphur dioxide


2017 ◽  
Vol 28 (2) ◽  
pp. 341
Author(s):  
Monica Duque Quintero ◽  
Ricardo Rosero Noguera ◽  
Marta Olivera Ángel

The aim of this study was to determine the digestion of dry matter (MS), crude protein (PC) and amino acids (AA) in dairy cows. Two cannulated cows were used for the determination of passage rate (Kp), in situ ruminal degradability (DR) and intestinal digestibility (DI) by abomasal catheter. The data to calculate Kp was analyzed with NLIN procedure by SAS, and descriptive statistics for DR and DI of MS, PC and AA from Kikuyu grass (Pennisetum clandestinum), a commercial supplement and two sources of rumen-protected AA. The study showed a Kp and a ruminal retention time of 0.036 h-1 and 27.4 h. The highest values from DR of MS and CP were from Kikuyu grass (69.0 and 61.8%) and concentrate (84.7 and 77.2%), followed by MetP (60.2 and 66.7%) and LysP (6.72 and 11.4%). The highest percentages of rumen indegradable amino acids (AADR) were from Kikuyu and concentrate, varying between 58.7 and 68% in forage, and 76.1 and 82.9% in the concentrate. The DR was 11.5 and 65.8% in LysP and MetP, respectively. The DI of AA (%AADR) varied between 42.3 and 77.4% for Kikuyu and 42.2 and 59.3% for concentrate. The values for the protected amino acids were 42.1 for LysP and 58.6 for MetP. 


2020 ◽  
Vol 43 ◽  
pp. e50856
Author(s):  
Doaa Elgohary Hanafy ◽  
Dina Mohamed Hamoda ◽  
Ibrahim Mohamed Khattab ◽  
Ahmed Abdelkader Aboamer

In situ bags made from microfiber fabrics have a greater surface area and filtration efficiency that retains the fine particles and reduce the variation in the results. Also, it is more durable than that made from traditional fabrics. This work aimed to study the effect of filament denier on the performance of in situ bags. Two polyester microfilaments with 0.4 and 0.7 deniers were used in manufacturing of four fabrics. Physical and mechanical properties of manufactured fabrics were measured before and after incubation to show the efficiency of the manufactured samples. In vitro trail was conducted to estimate ruminal degradability after 24 and 48 hours for three feedstuffs using three cannulated rams as replicates. The mechanical properties of manufactured in situ bags were significantly affected with both denier per filament and weft densities. According to the statistical analysis of radar chart, sample 2 remarked the highest value which achieved the acceptable ruminal dry matter disappearance compared with Ankom bags in different incubation times.


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