scholarly journals Human VE-Cadherin Fusion Protein as an Artificial Extracellular Matrix Enhancing the Proliferation and Differentiation Functions of Endothelial Cell

2016 ◽  
Vol 17 (3) ◽  
pp. 756-766 ◽  
Author(s):  
Ke Xu ◽  
Qizhi Shuai ◽  
Xiaoning Li ◽  
Yan Zhang ◽  
Chao Gao ◽  
...  
Biomaterials ◽  
2010 ◽  
Vol 31 (20) ◽  
pp. 5287-5296 ◽  
Author(s):  
Xiao-Shan Yue ◽  
Yuta Murakami ◽  
Toshiyuki Tamai ◽  
Masato Nagaoka ◽  
Chong-Su Cho ◽  
...  

Soft Matter ◽  
2021 ◽  
Author(s):  
Archita Sharma ◽  
Pooja Sharma ◽  
Sangita Roy

The phenomenal advancement in regenerative medicines have led to the development of bioinspired materials to fabricate biomimetic artificial extracellular matrix (ECM) to support cellular survival, proliferation, and differentiation. Researchers have...


1997 ◽  
Vol 78 (05) ◽  
pp. 1392-1398 ◽  
Author(s):  
A Schneider ◽  
M Chandra ◽  
G Lazarovici ◽  
I Vlodavsky ◽  
G Merin ◽  
...  

SummaryPurpose: Successful development of a vascular prosthesis lined with endothelial cells (EC) may depend on the ability of the attached cells to resist shear forces after implantation. The present study was designed to investigate EC detachment from extracellular matrix (ECM) precoated vascular prostheses, caused by shear stress in vitro and to test the performance of these grafts in vivo. Methods: Bovine aortic endothelial cells were seeded inside untreated polytetrafluoro-ethylene (PTFE) vascular graft (10 X 0.6 cm), PTFE graft precoated with fibronectin (FN), or PTFE precoated with FN and a naturally produced ECM (106 cells/graft). Sixteen hours after seeding the medium was replaced and unattached cells counted. The strength of endothelial cell attachment was evaluated by subjecting the grafts to a physiologic shear stress of 15 dynes/cm2 for 1 h. The detached cells were collected and quantitated. PTFE or EC preseeded ECM coated grafts were implanted in the common carotid arteries of dogs. Results: While little or no differences were found in the extent of endothelial cell attachment to the various grafts (79%, 87% and 94% of the cells attached to PTFE, FN precoated PTFE, or FN+ECM precoated PTFE, respectively), the number of cells retained after a shear stress was significanly increased on ECM coated PTFE (20%, 54% and 85% on PTFE, FN coated PTFE, and FN+ECM coated PTFE, respectively, p <0.01). Implantation experiments in dogs revealed a significant increase in EC coverage and a reduced incidence of thrombus formation on ECM coated grafts that were seeded with autologous saphenous vein endothelial cells prior to implantation. Conclusion: ECM coating significantly increased the strength of endothelial cell attachment to vascular prostheses subjected to shear stress. The presence of adhesive macromolecules and potent endothelial cell growth promoting factors may render the ECM a promising substrate for vascular prostheses.


Nanomaterials ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 21
Author(s):  
Mina Keshvardoostchokami ◽  
Sara Seidelin Majidi ◽  
Peipei Huo ◽  
Rajan Ramachandran ◽  
Menglin Chen ◽  
...  

Many types of polymer nanofibers have been introduced as artificial extracellular matrices. Their controllable properties, such as wettability, surface charge, transparency, elasticity, porosity and surface to volume proportion, have attracted much attention. Moreover, functionalizing polymers with other bioactive components could enable the engineering of microenvironments to host cells for regenerative medical applications. In the current brief review, we focus on the most recently cited electrospun nanofibrous polymeric scaffolds and divide them into five main categories: natural polymer-natural polymer composite, natural polymer-synthetic polymer composite, synthetic polymer-synthetic polymer composite, crosslinked polymers and reinforced polymers with inorganic materials. Then, we focus on their physiochemical, biological and mechanical features and discussed the capability and efficiency of the nanofibrous scaffolds to function as the extracellular matrix to support cellular function.


1992 ◽  
Vol 202 (2) ◽  
pp. 316-325 ◽  
Author(s):  
Daniele Toru-Delbauffe ◽  
Denise Baghdassarian ◽  
Dominique Both ◽  
Rozenn Bernard ◽  
Pierre Rouget ◽  
...  

2009 ◽  
Vol 20 (9) ◽  
pp. 1759-1764 ◽  
Author(s):  
Makiko Nakamura ◽  
Kumiko Yamaguchi ◽  
Masayasu Mie ◽  
Makoto Nakamura ◽  
Keiichi Akita ◽  
...  

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