A Method for the Accurate Quantification of Gas Streams by Online Mass Spectrometry

2021 ◽  
Author(s):  
Edwing Alexander Velasco-Rozo ◽  
Luz Marina Ballesteros-Rueda ◽  
Víctor Gabriel Baldovino-Medrano
Keyword(s):  
Mass Spectrometry ◽  
Gas Streams ◽  
Accurate Quantification

scholarly journals Characterization and Quantification of Selenoprotein P: Challenges to Mass Spectrometry

2021 ◽  
Vol 22 (12) ◽  
pp. 6283
Author(s):  
Jérémy Lamarche ◽  
Luisa Ronga ◽  
Joanna Szpunar ◽  
Ryszard Lobinski
Keyword(s):  
Mass Spectrometry ◽  
State Of The Art ◽  
Animal Health ◽  
Primary Standard ◽  
Selenoprotein P ◽  
Primary Standards ◽  
Analytical Approaches ◽  
Careful Investigation ◽  
Accurate Quantification

Selenoprotein P (SELENOP) is an emerging marker of the nutritional status of selenium and of various diseases, however, its chemical characteristics still need to be investigated and methods for its accurate quantitation improved. SELENOP is unique among selenoproteins, as it contains multiple genetically encoded SeCys residues, whereas all the other characterized selenoproteins contain just one. SELENOP occurs in the form of multiple isoforms, truncated species and post-translationally modified variants which are relatively poorly characterized. The accurate quantification of SELENOP is contingent on the availability of specific primary standards and reference methods. Before recombinant SELENOP becomes available to be used as a primary standard, careful investigation of the characteristics of the SELENOP measured by electrospray MS and strict control of the recoveries at the various steps of the analytical procedures are strongly recommended. This review critically discusses the state-of-the-art of analytical approaches to the characterization and quantification of SELENOP. While immunoassays remain the standard for the determination of human and animal health status, because of their speed and simplicity, mass spectrometry techniques offer many attractive and complementary features that are highlighted and critically evaluated.

scholarly journals A method for the highly accurate quantification of gas streams by on-line chromatography

2020 ◽  
Vol 1626 ◽  
pp. 461355
Author(s):  
Víctor Stivenson Sandoval-Bohorquez ◽  
Edwing Alexander Velasco Rozo ◽  
Víctor G. Baldovino-Medrano
Keyword(s):  
Gas Streams ◽  
On Line ◽  
Accurate Quantification

scholarly journals HILIC-Enabled 13C Metabolomics Strategies: Comparing Quantitative Precision and Spectral Accuracy of QTOF High- and QQQ Low-Resolution Mass Spectrometry

Metabolites ◽  
2019 ◽  
Vol 9 (4) ◽  
pp. 63 ◽  
Author(s):  
André Feith ◽  
Attila Teleki ◽  
Michaela Graf ◽  
Lorenzo Favilli ◽  
Ralf Takors
Keyword(s):  
Mass Spectrometry ◽  
Isotope Dilution Mass Spectrometry ◽  
Absolute Quantification ◽  
Spectral Accuracy ◽  
Time Resolved ◽  
13C Tracer ◽  
Accurate Quantification ◽  
Tracer Experiments

Dynamic 13C-tracer-based flux analyses of in vivo reaction networks still require a continuous development of advanced quantification methods applying state-of-the-art mass spectrometry platforms. Utilizing alkaline HILIC chromatography, we adapt strategies for a systematic quantification study in non- and 13C-labeled multicomponent endogenous Corynebacterium glutamicum extracts by LC-QTOF high resolution (HRMS) and LC-QQQ tandem mass spectrometry (MS/MS). Without prior derivatization, a representative cross-section of 17 central carbon and anabolic key intermediates were analyzed with high selectivity and sensitivity under optimized ESI-MS settings. In column detection limits for the absolute quantification range were between 6.8–304.7 (QQQ) and 28.7–881.5 fmol (QTOF) with comparable linearities (3–5 orders of magnitude) and enhanced precision using QQQ-MRM detection. Tailor-made preparations of uniformly (U)13C-labeled cultivation extracts for isotope dilution mass spectrometry enabled the accurate quantification in complex sample matrices and extended linearities without effect on method parameters. Furthermore, evaluation of metabolite-specific m+1-to-m+0 ratios (ISR1:0) in non-labeled extracts exhibited sufficient methodical spectral accuracies with mean deviations of 3.89 ± 3.54% (QTOF) and 4.01 ± 3.01% (QQQ). Based on the excellent HILIC performance, conformity analysis of time-resolved isotopic enrichments in 13C-tracer experiments revealed sufficient spectral accuracy for QQQ-SIM detection. However, only QTOF-HRMS ensures determination of the full isotopologue space in complex matrices without mass interferences.

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